Targeting of protein phosphatases PP2A and PP2B to the C-terminus of the L-type calcium channel Ca v1.2.

abstract：:A cell-free system for synthesizing protein from wheat germ was used to translate the messenger RNA extracted from 16-day embryonic chick calvaria. A part of the product had properties similar to collagenous peptides and served as a substrate for prolyl hydroxylase, an enzyme specific for collagen. The level of potass...

journal_title：Biochemistry

authors： Benveniste K,Wilczek J,Ruggieri A,Stern R

更新日期：1976-02-24 00:00:00

abstract：:The amide hydrogens that are exposed to solvent in the high-resolution X-ray structures of ubiquitin, FK506-binding protein, chymotrypsin inhibitor 2, and rubredoxin span a billion-fold range in hydroxide-catalyzed exchange rates which are predictable by continuum dielectric methods. To facilitate analysis of transien...

journal_title：Biochemistry

authors： LeMaster DM,Anderson JS,Hernández G

更新日期：2009-10-06 00:00:00

abstract：:Protein methylation is a common post-translational modification with diverse biological functions. Methyllysine reader proteins are increasingly a focus of epigenetics research and play important roles in regulating many cellular processes. These reader proteins are vital players in development, cell cycle regulation,...

journal_title：Biochemistry

authors： Milosevich N,Hof F

更新日期：2016-03-22 00:00:00

abstract：:Aquifex aeolicus 3-deoxy-d-manno-octulosonate 8-phosphate synthase (KDO8PS) is active with a variety of different divalent metal ions bound in the active site. The Cd(2+), Zn(2+), and Cu(2+) substituted enzymes display similar values of k(cat) and similar dependence of K(m)(PEP) and K(m)(A5P) on both substrate and pro...

journal_title：Biochemistry

authors： Kona F,Tao P,Martin P,Xu X,Gatti DL

更新日期：2009-04-28 00:00:00

abstract：:We have mapped the interface between the two transmembrane alpha-helices in the membrane domain of the Escherichia coli enzyme leader peptidase by analyzing disulfides formed between pairs of cysteine residues introduced near their respective periplasmic ends. The interface is formed primarily from aliphatic amino aci...

journal_title：Biochemistry

authors： Whitley P,Nilsson L,von Heijne G

更新日期：1993-08-24 00:00:00

abstract：:Guanidinium chloride- (GdmCl-) induced unfolding of beta-lactamase has been investigated by a combination of size-exclusion chromatography (SEC-FPLC) and usual optical methods. It has been shown that at low temperatures this protein unfolds through two equilibrium intermediates. The first of these intermediates is the...

journal_title：Biochemistry

authors： Uversky VN,Ptitsyn OB

更新日期：1994-03-15 00:00:00

abstract：:Amyloid deposition of human islet amyloid polypeptide (hIAPP) in the islets of Langerhans is closely associated with the pathogenesis of type II diabetes mellitus. Despite substantial evidence linking amyloidogenic hIAPP to loss of β-cell mass and decreased pancreatic function, the molecular mechanism of hIAPP cytotox...

journal_title：Biochemistry

authors： Sasahara K,Morigaki K,Okazaki T,Hamada D

更新日期：2012-09-04 00:00:00

abstract：:The light-driven enzyme NADPH:protochlorophyllide oxidoreductase (POR) catalyzes the reduction of protochlorophyllide (Pchlide) to chlorophyllide (Chlide), a key regulatory step in the chlorophyll biosynthesis pathway. As POR is light activated, it allows intermediates in the reaction pathway to be observed by initiat...

journal_title：Biochemistry

authors： Heyes DJ,Ruban AV,Hunter CN

更新日期：2003-01-21 00:00:00

abstract：:Regulation of adenylate cyclase by alpha 2 adrenergic receptors requires the inhibitory guanine nucleotide binding protein Ni. A role for this protein has also been suggested in the high-affinity binding of agonists to the alpha 2 receptor. We recently reported that alkaline treatment can selectively inactivate alpha ...

journal_title：Biochemistry

authors： Kim MH,Neubig RR

更新日期：1987-06-16 00:00:00

abstract：:A cyclic nucleotide phosphodiesterase was extensively purified from the 100000g supernatant fraction of human platelets. The purification was 2500-3000-fold with 30% recovery of activity. The enzyme was isolated by DEAE-cellulose chromatography followed by adsorption to blue dextran-Sepharose and elution with cAMP. Th...

journal_title：Biochemistry

authors： Grant PG,Colman RW

更新日期：1984-04-10 00:00:00

abstract：:Previously, we have described significant effects of human insulin on glucose metabolism in the yeast Saccharomyces cerevisiae under conditions of growth limitation. These regulations apparently rely on a transmembrane receptor capable of binding human insulin and responding by tyrosine/serine phosphorylation of a spe...

journal_title：Biochemistry

authors： Müller G,Grey S,Jung C,Bandlow W

更新日期：2000-02-15 00:00:00

abstract：:A short chain dehydrogenase like l-threonine 3-dehydrogenase (SDR-TDH) from metagenome data (mtTDH) was identified by database mining. Its enzymatic properties suggested that mtTDH has unique characteristics relative to other SDR-TDHs, including two mesophilic and thermophilic SDR-TDHs identified in this study. The ac...

journal_title：Biochemistry

authors： Motoyama T,Nakano S,Yamamoto Y,Tokiwa H,Asano Y,Ito S

更新日期：2017-10-31 00:00:00

abstract：:The interaction of oxidized nicotine adenine dinucleotide phosphate dependent isocitrate dehydrogenase (from pig heart) with (RS)-3-bromo-2-ketoglutarate was investigated in an effort to evaluate the reagent's potential as a selective reagent for alpha-ketoglutarate binding sites. The enzyme is rapidly inactivated by ...

journal_title：Biochemistry

authors： Hartman FC

更新日期：1981-02-17 00:00:00

abstract：:Phosphorylation by ATP of E.*Ca2 (sarcoplasmic reticulum vesicles (SRV) with bound 45Ca2+) during 5-10 ms leads to the occlusion of 2 *Ca2+/EPtot [quench by ethylene glycol bis(beta-aminoethyl ether)-N,N,N',N'-tetraacetic acid (EGTA) alone] in both "empty" (10 microM free Ca2+in) or "loaded" SRV (20-40 mM free Ca2+in)...

journal_title：Biochemistry

authors： Khananshvili D,Jencks WP

更新日期：1988-04-19 00:00:00

abstract：:The role of Tyr-14 of 3-oxo-delta 5-steroid isomerase (KSI) was probed by analysis of the spectra of 3-amino-1,3,5(10)-estratrien-17 beta-ol (4) and equilenin (5) bound to the active site of KSI. The ultraviolet spectrum of 4 bound to KSI is identical to that for 4 in neutral solution. This observation indicates that ...

journal_title：Biochemistry

authors： Zeng BF,Bounds PL,Steiner RF,Pollack RM

更新日期：1992-02-11 00:00:00

abstract：:RNAs are involved in myriad cellular events. In general, RNA function is affected by cellular conditions. For instance, molecular crowding promotes RNA folding through compaction of the RNA. Metabolites generally destabilize RNA secondary structure, which improves RNA folding cooperativity and increases the fraction o...

journal_title：Biochemistry

authors： Yamagami R,Huang R,Bevilacqua PC

更新日期：2019-09-24 00:00:00

abstract：:The product of transesterification of phospholipid acyl chains and unesterified cholesterol (UC) by the enzyme lecithin:cholesterol acyltransferase (LCAT) is cholesteryl ester (CE). Activation of LCAT by apolipoprotein (apo) A-I on nascent (discoidal) high-density lipoproteins (HDL) is essential for formation of matur...

journal_title：Biochemistry

authors： Jones MK,Catte A,Li L,Segrest JP

更新日期：2009-12-01 00:00:00

abstract：:Annexin A2 is a member of the annexin family of Ca(2+)-dependent lipid binding proteins and believed to be engaged in membrane transport processes in a number of cell types. In small intestinal enterocytes, we localized annexin A2 to the brush border region, where it was found mainly on the lumenal side of the microvi...

journal_title：Biochemistry

authors： Danielsen EM,van Deurs B,Hansen GH

更新日期：2003-12-16 00:00:00

abstract：:G-Actin is a globular protein (Mr 42 300) known to have three cysteine residues that are at least partially exposed and chemically reactive (Cys-10, -284, and -374). When G-actin was reacted with 3-bromo-1,1,1-trifluoropropanone, three resolvable 19F resonances were observed in the 19F NMR spectrum. This fluorinated G...

journal_title：Biochemistry

authors： Brauer M,Sykes BD

更新日期：1986-04-22 00:00:00

abstract：:Acyl carrier protein (ACP) is the central player in fatty acid (FA) biosynthesis. It covalently binds all FA intermediates and presents them to the enzymes needed for elongation. Bacterial ACP must interact with a large number of proteins, which raises the question of how different acyl-ACPs are recognized and disting...

journal_title：Biochemistry

authors： Chan DI,Tieleman DP,Vogel HJ

更新日期：2010-04-06 00:00:00

abstract：:Hydrogen exchange of the individual amide protons of alanine-90 (F5), glutamine-91 (F6), serine-92 (F7), and histidine-93 (F8) residues in cyanometmyoglobin of sperm whale has been studied by 1H nuclear magnetic resonance spectroscopy at 360 MHz. The amide proton resonance of F5, F6, and F7 have been assigned by use o...

journal_title：Biochemistry

authors： Kumar NV,Kallenbach NR

更新日期：1985-12-17 00:00:00

abstract：:Further to our studies on the Escherichia coli 30S ribosomal subunit, the detailed surface topography of both 50S subunits and 70S ribosomes has been investigated by using iodination catalyzed by immobilized lactoperoxidase as the surface probe. In the 50S subunit, only proteins L2, L5, L10, and L11 were iodinated to ...

journal_title：Biochemistry

authors： Maly P,Wower J,Zobawa M,Brimacombe R

更新日期：1983-06-21 00:00:00

abstract：:The septins are a conserved family of guanosine-5'-triphosphate (GTP)-binding proteins. In mammals they are involved in a variety of cellular processes, such as cytokinesis, exocytosis, and vesicle trafficking. Specifically, SEPT4 has also been shown to be expressed in both human colorectal cancer and malignant melano...

journal_title：Biochemistry

authors： Garcia W,de Araújo AP,Neto Mde O,Ballestero MR,Polikarpov I,Tanaka M,Tanaka T,Garratt RC

更新日期：2006-11-21 00:00:00

abstract：:Alpha-synuclein modulates dopamine homeostasis in dopamine-producing neurons of substantia nigra, partly through regulation of human dopamine transporter (hDAT) activity. To identify the underlying mechanisms, we disrupted the modulation of hDAT activity by wild-type (wt) alpha-synuclein, and its familial Parkinson's ...

journal_title：Biochemistry

authors： Wersinger C,Vernier P,Sidhu A

更新日期：2004-02-10 00:00:00

abstract：:The relaxin-like factor (RLF) is a circulating hormone that is synthesized in the gonads of mammals and released into the bloodstream. The distribution of its receptor and a trace of cross-reactivity to relaxin receptors implied that this relatively new factor is more relaxin- than insulin-like. The chemical synthesis...

journal_title：Biochemistry

authors： Büllesbach EE,Schwabe C

更新日期：1999-03-09 00:00:00

abstract：:Folding experiments have suggested that some proteins have kinetic intermediates with a non-native structure. A simple G ̅o model does not explain such non-native intermediates. Therefore, the folding energy landscape of proteins with non-native intermediates should have characteristic properties. To identify such pro...

journal_title：Biochemistry

authors： Sakurai K,Fujioka S,Konuma T,Yagi M,Goto Y

更新日期：2011-07-26 00:00:00

abstract：:Isotope exchange studies show that beef liver fructokinase has a random kinetic mechanism in which release of fructose from the enzyme is slower than that catalytic reaction. The stickiness of fructose in the presence of MgATP is confirmed by isotope partition studies, which show it to be released 0.53 times as fast a...

journal_title：Biochemistry

authors： Raushel FM,Cleland WW

更新日期：1977-05-17 00:00:00

abstract：:Prolyl endopeptidase (Prep) is a member of the prolyl peptidase family and is of interest because of its unique biochemistry and connections to cognitive function. Using an unbiased mass spectrometry (MS)-based peptidomics platform, we identified Prep-regulated peptides in the central nervous system (CNS) of mice by m...

journal_title：Biochemistry

authors： Nolte WM,Tagore DM,Lane WS,Saghatelian A

更新日期：2009-12-22 00:00:00

abstract：:A nucleoside triphosphate binding site on calf thymus RNA polymerase II was identified by using photoaffinity analogues of adenosine 5'-triphosphate and guanosine 5'-triphosphate. Both radiolabeled 8-azidoadenosine 5'-triphosphate (8-N3ATP) and radiolabeled 8-azidoguanosine 5'-triphosphate (8-N3GTP) bound to a single ...

journal_title：Biochemistry

authors： Freund E,McGuire PM

更新日期：1986-01-14 00:00:00

abstract：:Avoidance of over-reduction of the chloroplast ferredoxin pool is of paramount importance for plants in avoiding oxidative stress. The redox state of this pool can be controlled through regulation of the thylakoid electron transport chain. A model is presented for regulation of this chain via a thiol reduction mechani...

journal_title：Biochemistry

authors： Johnson GN

更新日期：2003-03-18 00:00:00