Peptidomics of prolyl endopeptidase in the central nervous system.

abstract：:Our previous studies demonstrated that the first 1000 amino acid residues (the βα1 domain) of human apolipoprotein (apo) B-100, termed apoB:1000, are required for the initiation of lipoprotein assembly and the formation of a monodisperse stable phospholipid (PL)-rich particle. The objectives of this study were (a) to ...

journal_title：Biochemistry

authors： Manchekar M,Kapil R,Sun Z,Segrest JP,Dashti N

更新日期：2017-08-08 00:00:00

abstract：:A debilitating complication of long-term hemodialysis is the deposition of beta-2-microglobulin (beta2m) as amyloid plaques in the joint space. We have recently shown that Cu(2+) can be a contributing, if not causal, factor at concentrations encountered during dialysis therapy. The basis for this effect is destabiliza...

journal_title：Biochemistry

authors： Eakin CM,Knight JD,Morgan CJ,Gelfand MA,Miranker AD

更新日期：2002-08-27 00:00:00

abstract：:Bean-deleterious Pseudomonas A214 produced the extracellular yellow-green, fluorescent siderophore [microbial iron(III) transport agent] pseudobactin A214 under iron-limiting conditions. Pseudobactin A214 has a molecular formula of C46H64N13O22 and a molecular mass of 1151 g/mol. Pseudobactin A214 contained an N-block...

journal_title：Biochemistry

authors： Buyer JS,Wright JM,Leong J

更新日期：1986-09-23 00:00:00

abstract：:trans-3-Chloroacrylic acid dehalogenase (CaaD) converts trans-3-chloroacrylic acid to malonate semialdehyde by the addition of H(2)O to the C-2, C-3 double bond, followed by the loss of HCl from the C-3 position. Sequence similarity between CaaD, an (alphabeta)(3) heterohexamer (molecular weight 47,547), and 4-oxalocr...

journal_title：Biochemistry

authors： Azurmendi HF,Wang SC,Massiah MA,Poelarends GJ,Whitman CP,Mildvan AS

更新日期：2004-04-13 00:00:00

abstract：:35S-labeled derivatives of the insecticides nodulisporic acid and ivermectin were synthesized and demonstrated to bind with high affinity to a population of receptors in Drosophila head membranes that were previously shown to be associated with a glutamate-gated chloride channel. Nodulisporic acid binding was modeled ...

journal_title：Biochemistry

authors： Ludmerer SW,Warren VA,Williams BS,Zheng Y,Hunt DC,Ayer MB,Wallace MA,Chaudhary AG,Egan MA,Meinke PT,Dean DC,Garcia ML,Cully DF,Smith MM

更新日期：2002-05-21 00:00:00

abstract：:The stereochemical course of the phospho group transfer catalyzed by mutant (S102C) alkaline phosphatase from Escherichia coli was investigated by using 31P nuclear magnetic resonance spectroscopy. Transphosphorylation from 4-nitrophenyl (Rp)-[16O, 17O, 18O]phosphate to (S)-propane-1,2-diol occurs with overall retenti...

journal_title：Biochemistry

authors： Butler-Ransohoff JE,Kendall DA,Freeman S,Knowles JR,Kaiser ET

更新日期：1988-06-28 00:00:00

abstract：:Gamma-aminobutyric acid type A receptors (GABA(A) receptors) are ligand-gated chloride channels that play a central role in signal transmission within the mammalian central nervous system. Compounds that modulate specific GABA(A) receptor subtypes containing the delta-subunit are scarce but would be valuable research ...

journal_title：Biochemistry

authors： Lewis RW,Mabry J,Polisar JG,Eagen KP,Ganem B,Hess GP

更新日期：2010-06-15 00:00:00

abstract：:Methylated DNA binding protein 2 (MeCP2) is a methyl CpG binding protein whose key role is the recognition of epigenetic information encoded in DNA methylation patterns. Mutation or misregulation of MeCP2 function leads to Rett syndrome as well as a variety of other autism spectrum disorders. Here, we have analyzed in...

journal_title：Biochemistry

authors： Ghosh RP,Nikitina T,Horowitz-Scherer RA,Gierasch LM,Uversky VN,Hite K,Hansen JC,Woodcock CL

更新日期：2010-05-25 00:00:00

abstract：:A photoaffinity probe for corticotropin (ACTH) receptors was prepared by selective modification of the single tryptophan residue in ACTH. A new photoreactive agent, 2,4-dinitro-5-azidophenylsulfenyl chloride, was synthesized and used for introducing the photoreactive group into ACTH. 2,4-Dinitro-5-azidophenylsulfenyl-...

journal_title：Biochemistry

authors： Canova-Davis E,Ramachandran J

更新日期：1980-07-08 00:00:00

abstract：:Strong inward-rectifier K(+) (Kir) channels play a significant role in shaping the cardiac action potential: they help produce its long plateau and accelerate its rate of repolarization. Consequently, genetic deletion of the gene encoding the strongly rectifying K(+) channel IRK1 (Kir2.1) prolongs the cardiac action p...

journal_title：Biochemistry

authors： Xu Y,Lu Z

更新日期：2004-12-14 00:00:00

abstract：:Calmodulin (CaM), by mediating the stimulation of the activity of two adenylyl cyclases (ACs), plays a key role in integrating the cAMP and Ca(2+) signaling systems. These ACs, AC1 and AC8, by decoding discrete Ca(2+) signals can contribute to fine-tuning intracellular cAMP dynamics, particularly in neurons where they...

journal_title：Biochemistry

authors： Masada N,Schaks S,Jackson SE,Sinz A,Cooper DM

更新日期：2012-10-09 00:00:00

abstract：:Hepatic microsomal NADPH-cytochrome P-450 reductase was solubilized from rabbit liver microsomes in the presence of detergents and purified to homogeneity by column chromatography. The purified reductase had a molecular weight of 78 000 and contained 1 mol each of FAD and FMN per mol of enzyme. On reduction with NADPH...

journal_title：Biochemistry

authors： Iyanagi T,Anan FK,Imai Y,Mason HS

更新日期：1978-05-30 00:00:00

abstract：:Cystathionine β-synthase (CBS) catalyzes the first step in the transsulfuration pathway in mammals, i.e., the condensation of serine and homocysteine to produce cystathionine and water. Recently, we have reported a steady-state kinetic analysis of the three hydrogen sulfide (H(2)S)-generating reactions that are cataly...

journal_title：Biochemistry

authors： Singh S,Ballou DP,Banerjee R

更新日期：2011-01-25 00:00:00

abstract：:Effects of human insulin on glucose metabolism in the yeast Saccharomyces cerevisiae were studied in this report. Under two conditions of growth limitation (glucose-grown cells during transition to stationary phase or spheroplasts during incubation in synthetic glucose medium), human insulin (10 and 1 microM, respecti...

journal_title：Biochemistry

authors： Müller G,Rouveyre N,Crecelius A,Bandlow W

更新日期：1998-06-16 00:00:00

abstract：:Ultraviolet thermal denaturation studies substantiate our earlier hypothesis that substitution of a L-nucleotide residue for a D-nucleotide within a DNA duplex permits a stable structure in which all bases are paired through Watson-Crick hydrogen bonds (Damha, M. J., Giannaris, P. A., Marfey, P., & Reid, L. S. (1991) ...

journal_title：Biochemistry

authors： Damha MJ,Giannaris PA,Marfey P

更新日期：1994-06-28 00:00:00

abstract：:We have used resonance energy transfer to study the spatial relationship between Cys-98 of rabbit skeletal troponin C and Cys-133 of rabbit skeletal troponin I in the reconstituted ternary troponin complex. The donor was introduced by labeling either troponin C or troponin I with N-(iodoacetyl)-N'-(5-sulfo-1-naphthyl)...

journal_title：Biochemistry

authors： Tao T,Gowell E,Strasburg GM,Gergely J,Leavis PC

更新日期：1989-07-11 00:00:00

abstract：:Neuronal specification is regulated by the activity of transcription factors containing the basic helix-loop-helix motif (bHLH); these regulating proteins include, among others, the neurogenin (Ngn) family, related to the atonal family of genes. Neurogenin 1 (NGN1) is a 237-residue protein that contains a bHLH domain ...

journal_title：Biochemistry

authors： Aguado-Llera D,Goormaghtigh E,de Geest N,Quan XJ,Prieto A,Hassan BA,Gómez J,Neira JL

更新日期：2010-03-02 00:00:00

abstract：:The mutations spectra of cis-syn, trans-syn-I, (6-4), and Dewar pyrimidone photoproducts of the TT site of AATTAA and TATTAT in the (-) strand of a heteroduplex M13 vector were obtained in an excision and photoreversal repair deficient Escherichia coli host under SOS conditions. Oligonucleotides containing site-specif...

journal_title：Biochemistry

authors： Smith CA,Wang M,Jiang N,Che L,Zhao X,Taylor JS

更新日期：1996-04-02 00:00:00

abstract：:Factor VII (proconvertin) has been purified approximately 5 x 10(5)-fold from bovine plasma with an overall yield of 30%. The isolation procedure involves barium sulfate adsorption and elution, DEAE-Sephadex batchwise adsorption and elution, benzamidine-agarose column chromatography, heparin-agarose column chromatogra...

journal_title：Biochemistry

authors： Kisiel W,Davie EW

更新日期：1975-11-04 00:00:00

abstract：:The globular domain of chicken histone H1 (GH1) has been studied by 1H homonuclear and 1H-15N heteronuclear 2D NMR spectroscopy. After the full assignment of the proton and 15N resonances, the tertiary structure of GH1 was determined by an iterative procedure using distance geometry and restrained simulated annealing....

journal_title：Biochemistry

authors： Cerf C,Lippens G,Ramakrishnan V,Muyldermans S,Segers A,Wyns L,Wodak SJ,Hallenga K

更新日期：1994-09-20 00:00:00

abstract：:The functionality of the membrane-bound, ubiquinone-dependent pyruvate oxidase from the respiratory chain of Escherichia coli was reconstituted with a supported lipidic structure. The artificial structure was especially designed to allow the electrochemical control of the quinone pool through the lateral mobility of t...

journal_title：Biochemistry

authors： Marchal D,Pantigny J,Laval JM,Moiroux J,Bourdillon C

更新日期：2001-02-06 00:00:00

abstract：:A peptide consisting of the first 21 residues of human myelin basic protein (MBP) was synthesized. The N-terminal alanine of portions was blocked in separate experiments with alkyl carboxylates varying in size from 2 to 10 carbon atoms. The effects of these different alkyl carboxylates at the N-terminus on the seconda...

journal_title：Biochemistry

authors： Costentino M,Pritzker L,Boulias C,Moscarello MA

更新日期：1994-04-12 00:00:00

abstract：:A site-specific analog of ATP, 6,6'-dithiobis (inosinyl imidodiphosphate (S2P-PNP), inactivates the ATPase activities of myosin's proteolytic fragments, heavy meromyosin (HMM) and subfragment one (SF1), by formation of mixed disulfides between the 6 position of the purine ring and certain key cysteines. The stoichiome...

journal_title：Biochemistry

authors： Wagner PD,Yount RG

更新日期：1975-05-06 00:00:00

abstract：:The intense intrinsic fluorescence emissions from several clinically relevant camptothecin drugs have been exploited in order to determine (1) the structural basis of drug binding to lipid bilayers, (2) the lipid bilayer stability of each drug's alpha-hydroxylactone moiety, a pharmacophore which is essential for antit...

journal_title：Biochemistry

authors： Burke TG,Mishra AK,Wani MC,Wall ME

更新日期：1993-05-25 00:00:00

abstract：:The ferritin IRE, a highly conserved (96-99% in vertebrates) mRNA translation regulatory element in animal mRNA, was studied by molecular modeling (using MC-SYM and DOCKING) and by NMR spectroscopy. Cobalt(III) hexammine was used to model hydrated Mg2+. IRE isoforms in other mRNAs regulate mRNA translation or stabilit...

journal_title：Biochemistry

authors： Gdaniec Z,Sierzputowska-Gracz H,Theil EC

更新日期：1998-02-10 00:00:00

abstract：:The proton-translocating NADH-quinone oxidoreductase (NDH-1) of Paracoccus denitrificans is composed of 14 different subunits (Nqo1-Nqo14). Of these, seven subunits (Nqo7, Nqo8, and Nqo10-14) which are equivalent to the mitochondrial DNA-encoded subunits of complex I constitute the membrane segment of the enzyme compl...

journal_title：Biochemistry

authors： Kao MC,Di Bernardo S,Matsuno-Yagi A,Yagi T

更新日期：2003-04-22 00:00:00

abstract：:The EP3 subtype of prostaglandin E2 receptor transduces diverse physiological responses in mammalian tissues through signaling pathways coupled to heterotrimeric G proteins. Distinct cDNA clones encoding five isoforms of the EP3 receptor were isolated from a human uterus cDNA library. The human EP3 receptor isoforms d...

journal_title：Biochemistry

authors： An S,Yang J,So SW,Zeng L,Goetzl EJ

更新日期：1994-12-06 00:00:00

abstract：:The clade B/intracellular serpins protect cells from peptidase-mediated injury by forming covalent complexes with their targets. SERPINB12 is expressed in most tissues, especially at cellular interfaces with the external environment. This wide tissue distribution pattern is similar to that of granzyme A (GZMA). Becaus...

journal_title：Biochemistry

authors： Niehaus JZ,Miedel MT,Good M,Wyatt AN,Pak SC,Silverman GA,Luke CJ

更新日期：2015-11-17 00:00:00

abstract：:Syk, a nonreceptor protein-tyrosine kinase, is activated by both oxidative and osmotic stress and plays different roles in the transduction of stress signals. In this study, the regulation of oxidative and osmotic stress induced Syk activation was investigated utilizing Syk-negative DT40 cells, expressing various Syk ...

journal_title：Biochemistry

authors： Qin S,Kurosaki T,Yamamura H

更新日期：1998-04-21 00:00:00

abstract：:The ferlin family proteins have emerged as multi-C2 domain regulators of calcium-triggered membrane fusion and fission events. While initially determined to share many of the features of members of the synaptotagmin family of calcium sensors, ferlins in more recent studies have been found to interact directly with non...

journal_title：Biochemistry

authors： Johnson CP

更新日期：2017-12-12 00:00:00