Three-dimensional model for the membrane domain of Escherichia coli leader peptidase based on disulfide mapping.

abstract：:NMR spectroscopic experiments were performed to study the structure of synthetic peptides identical to two extracellular regions of the human thrombin receptor. The smaller molecule, comprising 14 amino acids, was biologically active and was equivalent to the "tethered ligand" exposed after cleavage of the receptor by...

journal_title：Biochemistry

authors： Smith KJ,Trayer IP,Grand RJ

更新日期：1994-05-24 00:00:00

abstract：:Prolyl hydroxylase is a glycoprotein containing two nonidentical subunits, alpha and beta. The alpha subunit of prolyl hydroxylase isolated from 13-day-old chick embryos contains a single high mannose oligosaccharide having seven mannosyl residues. Two forms of alpha subunit have been shown to exist in enzyme purified...

journal_title：Biochemistry

authors： Kedersha NL,Tkacz JS,Berg RA

更新日期：1985-10-08 00:00:00

abstract：:Comparative studies on the salt titration and the related kinetics for poly(dG-dC) X poly(dG-dC) in pH 7.0 and 3.8 solutions clearly suggest that base protonation facilitates the kinetics of B-Z interconversion although the midpoint for such a transition in acidic solution (2.0-2.1 M NaCl) is only slightly lower than ...

journal_title：Biochemistry

authors： Chen FM

更新日期：1984-12-04 00:00:00

abstract：:Three spin-labeled derivatives of the voltage-gated peptide alamethicin were prepared with nitroxides at the C-terminal phenyalaninol, and at positions 9 and 15 in the amino acid sequence. In addition, three spin-labeled derivatives of an analog of alamethicin where alpha-methylalanine residues are replaced by leucine...

journal_title：Biochemistry

authors： Barranger-Mathys M,Cafiso DS

更新日期：1996-01-16 00:00:00

abstract：:Site-directed mutagenesis of the aspartate receptor of Salmonella typhimurium (Tars) at serine 68, a residue located within the aspartate binding pocket and at the subunit interface, identified this residue as an allosteric switch in this receptor. Substitutions at this position can affect both the type and degree of ...

journal_title：Biochemistry

authors： Kolodziej AF,Tan T,Koshland DE Jr

更新日期：1996-11-26 00:00:00

abstract：:Trytophanase from Bacillus alvei was resolved from its coenzyme, pyridoxal phosphate, by treatment with cysteine followed by column chromatography. Spectrophotometric titration of apoenzyme with pyridoxal-P showed 1 mol of pyridoxal-P bound per 52,000 g of enzyme. Kinetic analysis of coenzyme binding showed hyperbolic...

journal_title：Biochemistry

authors： Isom HC,DeMoss RD

更新日期：1975-09-23 00:00:00

abstract：:The electrostatic binding strength of water-soluble proteins having either an excess positive (cytochrome c) or negative (beta-lactoglobulin) electric charge to oppositely charged supported planar bilayers (SPBs) was studied as a function of the bilayer phase state (fluid or gel phase) by IR-ATR spectroscopy. The bila...

journal_title：Biochemistry

authors： Käsbauer M,Bayerl TM

更新日期：1999-11-16 00:00:00

abstract：:Esterification of retinol (vitamin A alcohol) with long-chain fatty acids by lecithin-retinol acyltransferase (LRAT) is an important step in both the absorption and storage of vitamin A. Retinol in cells is bound by either cellular retinol binding protein (CRBP), present in most tissues including liver, or cellular re...

journal_title：Biochemistry

authors： Herr FM,Ong DE

更新日期：1992-07-28 00:00:00

abstract：:Stability increments of terminal unpaired nucleotides (dangling ends) and terminal base pairs on the core helixes AUGCAU and UGCGCA are reported. Enthalpy, entropy, and free energy changes of helix formation were measured spectrophotometrically for 18 oligoribonucleotides containing the core sequences. The results ind...

journal_title：Biochemistry

authors： Sugimoto N,Kierzek R,Turner DH

更新日期：1987-07-14 00:00:00

abstract：:TP40 is a chimeric protein containing transforming growth factor alpha (TGF-alpha) at the N-terminus and a derivative of a 40,000-Da segment (PE40 delta cys) of Pseudomonas exotoxin (PE). PE40 delta cys contains domains Ib, II, and III of PE in which the cysteines are mutated to alanines. The rationale for inclusion o...

journal_title：Biochemistry

authors： Sanyal G,Marquis-Omer D,Gress JO,Middaugh CR

更新日期：1993-04-06 00:00:00

abstract：:We have isolated from rabbit liver three cDNA clones of 1400-1800 base pairs that hybridize selectively to RNA from animals treated with phenobarbital. The nucleotide sequences of the cDNAs have been determined. In the protein coding region the nucleotide sequences of two of the cDNAs are 88% homologous, and the third...

journal_title：Biochemistry

authors： Leighton JK,DeBrunner-Vossbrinck BA,Kemper B

更新日期：1984-01-17 00:00:00

abstract：:Membrane vesicles isolated from Escherichia coli ML 308--225 have been analyzed by crossed immunoelectrophoresis, and immunoprecipitates corresponding to the following cellular components have been identified: ATPase (EC 3.6.1,3), two or three NADH dehydrogenases (EC 1.6.99.3), D-lactate dehydrogenase (EC 1.1.1.27), g...

journal_title：Biochemistry

authors： Owen P,Kaback HR

更新日期：1979-04-17 00:00:00

abstract：:Further to our studies on the Escherichia coli 30S ribosomal subunit, the detailed surface topography of both 50S subunits and 70S ribosomes has been investigated by using iodination catalyzed by immobilized lactoperoxidase as the surface probe. In the 50S subunit, only proteins L2, L5, L10, and L11 were iodinated to ...

journal_title：Biochemistry

authors： Maly P,Wower J,Zobawa M,Brimacombe R

更新日期：1983-06-21 00:00:00

abstract：:Vc H-NOX (or VCA0720) is an H-NOX (heme-nitric oxide and oxygen binding) protein from facultative aerobic bacterium Vibrio cholerae. It shares significant sequence homology with soluble guanylyl cyclase (sGC), a NO sensor protein commonly found in animals. Similar to sGC, Vc H-NOX binds strongly to NO and CO with affi...

journal_title：Biochemistry

authors： Wu G,Liu W,Berka V,Tsai AL

更新日期：2013-12-31 00:00:00

abstract：:The channel of the purified acetylcholine receptor from Torpedo californica electric organ reconstituted in lipid vesicles was assayed by direct electrical recording using patch-clamp pipets. High-resistance seals were obtained by gentle suction of vesicles into the pipet or after the formation of lipid bilayers from ...

journal_title：Biochemistry

authors： Suarez-Isla BA,Wan K,Lindstrom J,Montal M

更新日期：1983-05-10 00:00:00

abstract：:The human immunodeficiency virus utilizes its gp41 fusion protein to mediate virus-cell membrane fusion. The conserved disulfide loop region in the gp41 hairpin conformation reverses the protein chain, such that the N-terminal heptad repeat and the C-terminal heptad repeat regions interact to form the six-helix bundle...

journal_title：Biochemistry

authors： Ashkenazi A,Merklinger E,Shai Y

更新日期：2012-09-04 00:00:00

abstract：:A conserved, crystallographically defined bile acid binding site was originally identified in the membrane domain of mammalian and bacterial cytochrome c oxidase (CcO). Current studies show other amphipathic molecules including detergents, fatty acids, steroids, and porphyrins bind to this site and affect the already ...

journal_title：Biochemistry

authors： Hiser C,Buhrow L,Liu J,Kuhn L,Ferguson-Miller S

更新日期：2013-02-26 00:00:00

abstract：:β-Lactamase inhibitors (BLIs) restore the efficacy of otherwise obsolete β-lactams. However, commercially available BLIs are not effective against metallo-β-lactamases (MBLs), which continue to be disseminated globally. One group of the most clinically important MBLs is the VIM family. The discovery of VIM-24, a natur...

journal_title：Biochemistry

authors： Mojica MF,Mahler SG,Bethel CR,Taracila MA,Kosmopoulou M,Papp-Wallace KM,Llarrull LI,Wilson BM,Marshall SH,Wallace CJ,Villegas MV,Harris ME,Vila AJ,Spencer J,Bonomo RA

更新日期：2015-05-26 00:00:00

abstract：:The linear noncompetitive inhibition of the pepsin-catalyzed hydrolysis of Ac-Phe-Phe-Gly at pH 2.1 by L-Ac-Phe, L-Ac-Phe-NH2, and L-Ac-Phe-OEt has been claimed to substantiate the ordered release of products specified by the amino-enzyme mechanism for pepsin action. According to this interpretation, the binding of in...

journal_title：Biochemistry

authors： Silver MS,Stoddard M

更新日期：1975-02-11 00:00:00

abstract：:The inducible isoform of nitric oxide synthase (iNOS) and three zinc tetrathiolate mutants (C104A, C109A, and C104A/C109A) were expressed in Escherichia coli and purified. The mutants were found by ICP-AES and the zinc-specific PAR colorimetric assay to be zinc free, whereas the wild-type iNOS zinc content was 0.38 +/...

journal_title：Biochemistry

authors： Mitchell DA,Erwin PA,Michel T,Marletta MA

更新日期：2005-03-29 00:00:00

abstract：:The crystal structure of a nitrogenase Fe protein single site deletion variant reveals a distinctly new conformation of the Fe protein and indicates that, upon binding of MgATP, the Fe protein undergoes a dramatic conformational change that is largely manifested in the rigid-body reorientation of the homodimeric Fe pr...

journal_title：Biochemistry

authors： Sen S,Igarashi R,Smith A,Johnson MK,Seefeldt LC,Peters JW

更新日期：2004-02-24 00:00:00

abstract：:To address the targeting of G protein-coupled receptors to caveolae-related lipid rafts (CLR), we studied the human B2 (B2R) and B1 (B1R) bradykinin receptor subtypes in HEK293 cells. CLR were enriched on the basis of their unique buoyant density and composition of cholesterol, caveolin-1, and flotillin-1 but not clat...

journal_title：Biochemistry

authors： Lamb ME,Zhang C,Shea T,Kyle DJ,Leeb-Lundberg LM

更新日期：2002-12-03 00:00:00

abstract：:Messenger RNA degradation is an important component of overall gene expression. During the final step of eukaryotic mRNA degradation, exoribonuclease 1 (Xrn1) carries out 5' → 3' processive, hydrolytic degradation of RNA molecules using divalent metal ion catalysis. To initiate studies of the 5' → 3' RNA decay machine...

journal_title：Biochemistry

authors： Langeberg CJ,Welch WRW,McGuire JV,Ashby A,Jackson AD,Chapman EG

更新日期：2020-04-21 00:00:00

abstract：:Incorporation of nonproteinogenic amino acids in small polypeptides synthesized by nonribosomal peptide synthetases (NRPS) significantly contributes to their biological activity. In these peptides, conversion of L-amino acids to the corresponding D-isomer is catalyzed by specialized NRPS modules that utilize an epimer...

journal_title：Biochemistry

authors： Linne U,Doekel S,Marahiel MA

更新日期：2001-12-25 00:00:00

abstract：:Transport of protons and solutes across mitochondrial membranes is essential for many physiological processes. However, neither the proton-pumping respiratory chain complexes nor the mitochondrial secondary active solute transport proteins have been characterized electrophysiologically in their native environment. In ...

journal_title：Biochemistry

authors： Watzke N,Diekert K,Obrdlik P

更新日期：2010-12-07 00:00:00

abstract：:Specificity of the binding of sterols and related compounds with purified F-protein (fusion protein) of the HVJ (Sendai virus) was studied by binding competition with [3H]cholesterol. Requirement for cholesterol or the A/B ring trans structure and nonrequirement for the 3-hydroxyl group were found in this binding. Bin...

journal_title：Biochemistry

authors： Asano K,Asano A

更新日期：1988-02-23 00:00:00

abstract：:Two-dimensional homonuclear and heteronuclear NMR and minimized potential energy calculations have been combined to define the structure of the antitumor agent mitomycin C (MC) cross-linked to deoxyguanosines on adjacent base pairs in the d(T1-A2-C3-G4-T5-A6).d(T7-A8-C9-G10-T11-A12) duplex. The majority of the mitomyc...

journal_title：Biochemistry

authors： Norman D,Live D,Sastry M,Lipman R,Hingerty BE,Tomasz M,Broyde S,Patel DJ

更新日期：1990-03-20 00:00:00

abstract：:The protein product of the Methanococcus jannaschii MJ0768 gene has been expressed in Escherichia coli, purified to homogeneity, and shown to catalyze the GTP-dependent addition of two l-glutamates to the l-lactyl phosphodiester of 7,8-didemethyl-8-hydroxy-5-deazariboflavin (F(420)-0) to form F(420)-0-glutamyl-glutama...

journal_title：Biochemistry

authors： Li H,Graupner M,Xu H,White RH

更新日期：2003-08-19 00:00:00

abstract：:The two most basic charge isomers of myelin basic protein (BP), components 1 and 2 (C1 and C2), which presumably differ in the degree of deamidation, were purified from bovine BP by cation-exchange chromatography. Two additional specific types of posttranslational modifications were introduced into the purified isomer...

journal_title：Biochemistry

authors： Cheifetz S,Boggs JM,Moscarello MA

更新日期：1985-09-10 00:00:00

abstract：:Reconstitution of highly purified aquaporin CHIP (channel-forming integral protein) into proteoliposomes was previously shown to confer high osmotic water permeability (Pf) to the membranes [Zeidel et al. (1992) Biochemistry 31, 7436-7440]. Here we report detailed ultrastructural, pharmacologic, and transport studies ...

journal_title：Biochemistry

authors： Zeidel ML,Nielsen S,Smith BL,Ambudkar SV,Maunsbach AB,Agre P

更新日期：1994-02-15 00:00:00