Abstract：
:Phenylalanine acts as an allosteric activator of the tetrahydropterin-dependent enzyme phenylalanine hydroxylase. Hydrogen/deuterium exchange monitored by mass spectrometry has been used to gain insight into local conformational changes accompanying activation of rat phenylalanine hydroxylase by phenylalanine. Peptides in the regulatory and catalytic domains that lie in the interface between these two domains show large increases in the extent of deuterium incorporation from solvent in the presence of phenylalanine. In contrast, the effects of phenylalanine on the exchange kinetics of a mutant enzyme lacking the regulatory domain are limited to peptides surrounding the binding site for the amino acid substrate. These results support a model in which the N-terminus of the protein acts as an inhibitory peptide, with phenylalanine binding causing a conformational change in the regulatory domain that alters the interaction between the catalytic and regulatory domains.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Li J,Dangott LJ,Fitzpatrick PFdoi
10.1021/bi1001294subject
Has Abstractpub_date
2010-04-20 00:00:00pages
3327-35issue
15eissn
0006-2960issn
1520-4995journal_volume
49pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract：:The interaction of serine protease (esterases) with 6-chloro-2-pyrones was investigated. Time-dependent inactivation of chymotrypsin, alpha-lytic protease, pig liver elastase, and cholinesterase was found with 3- and 5-benzyl-6-chloro-2-pyrone, as well as 3- and 5-methyl-6-chloro-2-pyrone. No inactivation was observed...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00282a034
更新日期：1983-06-21 00:00:00
abstract：:Peripheral benzodiazepine receptors (PBR), first described more than 20 years ago, have been attributed with many putative functions including ones in cellular proliferation and cellular respiration. Hence, it is quite conceivable that deregulation of this receptor could lead to pathology. We and others have reported ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi030251v
更新日期：2004-09-28 00:00:00
abstract：:Aspartate transcarbamoylase from Escherichia coli is a dodecameric enzyme consisting of two trimeric catalytic subunits and three dimeric regulatory subunits. The X-ray structure of this enzyme indicates that the side chains of His-41, Asp-100, and Asp-90 from one catalytic chain form interactions with the side chains...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00089a034
更新日期：1993-09-28 00:00:00
abstract：:Eukaryotic proteins with carboxyl-terminal CaaX motifs undergo three post-translational processing reactions-protein prenylation, endoproteolysis, and carboxymethylation. Two genes in yeast encoding CaaX endoproteases, AFC1 and RCE1, have been identified. Rce1p is solely responsible for proteolysis of yeast Ras protei...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi9923611
更新日期：2000-04-11 00:00:00
abstract：:Human apolipoprotein stimulation of sphingomyelin (SM) hydrolysis by sphingomyelinase from human skin fibroblasts has been studied. Apolipoproteins A-I, A-II, B, C-I, and E do not enhance sphingomyelin hydrolysis above control levels. In contrast, apoC-II stimulates sphingomyelin hydrolysis by approximately 2.5-fold. ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00363a036
更新日期：1986-07-29 00:00:00
abstract：:Ribosomal protein E-L30 unfolds reversibly at pH values between 7.0 and 4.5. Unfolding of the protein involves a fast and a slow equilibrium, which depend on the degree of protonation of His19 and His33. Both the fast equilibrium between protonated and deprotonated histidines and the slow equilibrium between folded an...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00391a050
更新日期：1987-08-25 00:00:00
abstract：:The prokaryotic DNA protection during starvation (Dps) proteins typically protect macromolecules against damaging agents via physical association with DNA and by oxidizing and sequestering iron. However, Deinococcus radiodurans Dps-1, which binds DNA with high affinity, fails to protect DNA against hydroxyl radicals d...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi300703x
更新日期：2012-08-21 00:00:00
abstract：:The ratio of the second-order rate constants for reduction of dihydroxyacetone phosphate (DHAP) and of the neutral truncated substrate glycolaldehyde (GLY) by glycerol 3-phosphate dehydrogenase (NAD (+), GPDH) saturated with NADH is (1.0 x 10 (6) M (-1) s (-1))/(8.7 x 10 (-3) M (-1) s (-1)) = 1.1 x 10 (8), which was u...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi8001743
更新日期：2008-04-22 00:00:00
abstract：:6-Methylsalicylic acid synthase, the multifunctional enzyme complex that catalyzes the biosynthesis of the tetraketide 6-methylsalicylic acid, was modified by thiol-specific inhibitors and cross-linking reagents. Treatment with 1,3-dibromopropan-2-one caused rapid enzyme inactivation and formation of cross-linked dime...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi960422e
更新日期：1996-09-24 00:00:00
abstract：:P450cam from Pseudomonas putida is the best characterized member of the vast family of cytochrome P450s, and it has long been believed to have a more rigid and closed active site relative to other P450s. Here we report X-ray structures of P450cam crystallized in the absence of substrate and at high and low [K(+)]. The...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi100183g
更新日期：2010-04-27 00:00:00
abstract：:The interactions of Hoechst 33377 (H1) with 20 different oligomeric duplexes have been investigated via spectrofluorometric titrations and/or thermal denaturation experiments. H1 is shown to form 2:1 complexes with dsDNA binding sites of at least four contiguous A/T base pairs. H1 is also shown to possess the rare abi...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0103415
更新日期：2001-05-29 00:00:00
abstract：:UvrA is the ATPase subunit of the DNA repair enzyme (A)BC excinuclease. The amino acid sequence of this protein has revealed, in addition to two zinc fingers, three pairs of nucleotide binding motifs each consisting of a Walker A and B sequence. We have conducted site-specific mutagenesis, ATPase kinetic analyses, and...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00230a004
更新日期：1991-04-23 00:00:00
abstract：:Membrane vesicles can be prepared from murine lymphoid cells by nitrogen cavitation and fractionated by sedimentation through nonlinear sucrose density gradients. Two subpopulations of membrane vesicles, PMI and PMII, can be distinguished on the basis of sedimentation rate. The subcellular distribution of adenylate an...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00652a026
更新日期：1976-04-06 00:00:00
abstract：:The recently identified Krokinobacter rhodopsin 2 (KR2) functions as a light-driven sodium ion pump. The structure of the retinal-binding pocket of KR2 offers important insights into the mechanisms of KR2, which has motif of Asn112, Asp116, and Gln123 (NDQ) that is common among sodium ion pump rhodopsins but is unique...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.6b00999
更新日期：2017-01-31 00:00:00
abstract：:Conformational alterations occurring in bovine alpha 2-macroglobulin (alpha 2M) resulting from proteolysis and nucleophilic modification have been monitored by UV difference spectra, circular dichroism, and changes in the fluorescence of 6-(p-toluidino)-2-naphthalenesulfonate (TNS) and bis(8-anilino-1-naphthalenesulfo...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00321a061
更新日期：1984-12-18 00:00:00
abstract：:The ability of avian-specific linker histone H5, and the globular domains of H5 (GH5) and H1(0) (GH1(0), to self-associate either free in solution or when bound to DNA was investigated. All three proteins underwent a salt-dependent increase in turbidity that may be indicative of nonspecific interactions. Dithiobis(suc...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi980716v
更新日期：1998-09-08 00:00:00
abstract：:Specific intra- and intermolecular quadruplex conformations of model G-DNA oligonucleotides have been identified from their translational and rotational diffusion coefficients in aqueous solution. The transport properties were determined by polarized and depolarized dynamic light scattering. A comparison with hydrodyn...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi990750p
更新日期：1999-09-21 00:00:00
abstract：:The turnover of the collagen triple-helical structure (collagenolysis) is a tightly regulated process in normal physiology and has been ascribed to a small number of proteases. Several members of the matrix metalloproteinase (MMPs) family possess collagenolytic activity, and the mechanisms by which these enzymes proce...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi062199j
更新日期：2007-03-27 00:00:00
abstract：:[1-Penicillamine,2-leucine]oxytocin is a conformationally restricted analogue of oxytoxin in which the half-cystine-1 and tyrosine-2 residues of the native hormone are replaced by half-penicillamine (beta, beta-dimethyl-half-cystine) and leucine, respectively. This analogue is a surprisingly potent oxytocin antagonist...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00513a018
更新日期：1981-05-12 00:00:00
abstract：:Aminopeptidases catalyze the hydrolysis of amino acid residues from the amino terminus of peptide substrates. They are found in most cells and tissues, and their activity has been implicated in myriad fundamental biochemical and physiological processes. Nevertheless, little is known about the structure of the aminopep...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00131a034
更新日期：1992-04-28 00:00:00
abstract：:A considerable body of data supports the model that the infectious agent (called a prion) which causes the transmissible spongiform encephalopathies is a replicating polypeptide devoid of nucleic acid. Prions are believed to propagate by changing the conformation of the normal cellular prion protein (PrPc) into an inf...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi982328z
更新日期：1999-03-16 00:00:00
abstract：:Pharaonis halorhodopsin (phR), the light-driven chloride ion pump from Natronobacterium pharaonis with C-terminal histidine tag, was expressed in Escherichia coli cells. The protein was solubilized with 0.1% n-dodecyl beta-D-maltopyranoside and purified with a nickel column. Removal of Cl- from the medium yields blue ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi011788g
更新日期：2002-02-19 00:00:00
abstract：:Apolipoprotein AIV is one of the principal apolipoproteins synthesized by the rat small intestine. We have cloned a cDNA encoding a portion of preapolipoprotein AIV mRNA. A kinetically fractionated cDNA probe highly enriched for the abundant intestinal mucosal mRNA sequences was used to screen a library of recombinant...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00265a007
更新日期：1982-10-26 00:00:00
abstract：:The proposal that microtubule dynamic instability results from stabilization of microtubule ends by tubulin-GDP-Pi subunits (where Pi is inorganic phosphate) [Melki et al. (1996) Biochemistry 35, 12038] was based on studies of GTP hydrolysis and microtubule assembly that showed that tubulin-GDP-Pi subunits can transie...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi972602+
更新日期：1998-09-15 00:00:00
abstract：:Amphotericin B (AmB) is a polyene macrolide antibiotic isolated from Streptomyces nodosus. The antifungal activity of AmB can be attributed to the formation of an ion-channel assembly in the presence of ergosterol (Erg), in which there are two different AmB-Erg orientations, parallel and antiparallel, as reported prev...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.6b00193
更新日期：2016-06-21 00:00:00
abstract：:Hydrogenases are metalloenzymes that catalyze the reversible reduction of protons at unusual metal centers. This Current Topic discusses recent advances in elucidating the steps involved in the biosynthesis of the complex metal cluster at the [FeFe]-hydrogenase (HydA) active site, known as the H-cluster. The H-cluster...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi500210x
更新日期：2014-07-01 00:00:00
abstract：:The structural and functional roles of two conserved active site residues, Arg-97 and Arg-132, in chicken muscle adenylate kinase (AK) were evaluated by site-directed mutagenesis in conjunction with one- and two-dimensional proton nuclear magnetic resonance (NMR), kinetics, and guanidine hydrochloride-induced denatura...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00142a022
更新日期：1992-07-14 00:00:00
abstract：:Polycyclic aromatic hydrocarbon (PAH)-DNA adducts pervert the execution or fidelity of enzymatic DNA transactions and cause mutations and cancer. Here, we examine the effects of intercalating PAH-DNA adducts on the religation reaction of vaccinia DNA topoisomerase, a prototypal type IB topoisomerase (TopIB), and the 3...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi060158h
更新日期：2006-06-20 00:00:00
abstract：:We have used diethyl pyrocarbonate (DEP), which carbethoxylates adenine bases, and dimethyl sulfate (DMS), which methylates guanine residues and single-stranded cytosines, to probe bases in open complexes between RNA polymerase and the lac UV5 promoter in vitro. We compared the kinetics of reactivity between bases in ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00436a040
更新日期：1989-05-16 00:00:00
abstract：:The EP3 subtype of prostaglandin E2 receptor transduces diverse physiological responses in mammalian tissues through signaling pathways coupled to heterotrimeric G proteins. Distinct cDNA clones encoding five isoforms of the EP3 receptor were isolated from a human uterus cDNA library. The human EP3 receptor isoforms d...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00252a016
更新日期：1994-12-06 00:00:00
