The Structure of the Bimolecular Complex between Amphotericin B and Ergosterol in Membranes Is Stabilized by Face-to-Face van der Waals Interaction with Their Rigid Cyclic Cores.

abstract：:In iron-deficient conditions, Azomonas macrocytogenes ATCC 12334 excretes a fluorescent siderophore called azoverdin, which is composed of a six-amino-acid peptide chain linked to a chromophore. Azoverdin chelates iron(III) very strongly, solubilizing it and transporting it back into the cells using an outer-membrane ...

journal_title：Biochemistry

authors： Wasielewski E,Atkinson RA,Abdallah MA,Kieffer B

更新日期：2002-10-15 00:00:00

abstract：:We have measured the rates and efficiencies of DNA unwinding (the number of ATP molecules hydrolyzed per DNA base pair unwound) catalyzed by the RecBC,RecBCD-K177Q (a site-directed mutant in the putative ATP-binding site in the RecD subunit), and RecBCD enzymes from Escherichia coli. The DNA unwinding rate was measure...

journal_title：Biochemistry

authors： Korangy F,Julin DA

更新日期：1994-08-16 00:00:00

abstract：:Aminoglycoside 3'-phosphotransferases [APH(3')s] are the most common cause of bacterial high-level resistance to aminoglycoside antibiotics in clinical isolates. A one-step affinity chromatography was used to purify APH(3') type Ia. The kinetic parameters for turnover of seven aminoglycosides and the corresponding min...

journal_title：Biochemistry

authors： Siregar JJ,Miroshnikov K,Mobashery S

更新日期：1995-10-03 00:00:00

abstract：:DNA ring closure experiments on short restriction fragments ( approximately 160 bp) bound by the TATA box binding protein (TBP) have demonstrated the formation of negative topoisomers, consistent with crystallographically observed TBP-induced DNA untwisting but in contrast to most previous results on topological effec...

journal_title：Biochemistry

authors： Kahn JD

更新日期：2000-04-04 00:00:00

abstract：:Glycogen synthase kinase 3β (GSK-3β) is a serine-threonine kinase belonging to the CMGC family that plays a key role in many biological processes, such as glucose metabolism, cell cycle regulation, and proliferation. Like most protein kinases, GSK-3β is regulated via multiple pathways and sites. We performed all-atom ...

journal_title：Biochemistry

authors： Buch I,Fishelovitch D,London N,Raveh B,Wolfson HJ,Nussinov R

更新日期：2010-12-28 00:00:00

abstract：:The cAMP-dependent protein kinase (cAPK) plays an essential role during differentiation and fruit morphogenesis in Dictyostelium discoideum. The presence of an open reading frame on the gene, pkaC (previously named either Dd PK2 or Dd PK3 by different groups), predicts a 73-kDa polypeptide with 54% similarity to the c...

journal_title：Biochemistry

authors： Anjard C,Etchebehere L,Pinaud S,Véron M,Reymond CD

更新日期：1993-09-21 00:00:00

abstract：:The seven serotypes (A-G) of botulinum neurotoxins (BoNTs) function through their proteolytic cleavage of one of three proteins (SNAP-25, Syntaxin, and VAMP) that form the SNARE complex required for synaptic vesicle fusion. The different BoNTs have very specific protease recognition requirements, between 15 and 50 ami...

journal_title：Biochemistry

authors： Arndt JW,Chai Q,Christian T,Stevens RC

更新日期：2006-03-14 00:00:00

abstract：:Pepstatin is a slow and tight-binding inhibitor of pepsin. Preincubating enzyme and inhibitor in H2O and in D2O in the absence of substrate generates an inverse solvent isotope effect of Dk = 0.69 +/- 0.06 on the apparent first-order rate constant for the decay in enzymatic activity. Proton inventory analysis of the i...

journal_title：Biochemistry

authors： Cho YK,Rebholz KL,Northrop DB

更新日期：1994-08-16 00:00:00

abstract：:Merozoite surface protein 2 (MSP2), an abundant glycosylphosphatidylinositol-anchored protein on the surface of Plasmodium falciparum merozoites, is a promising malaria vaccine candidate. MSP2 is intrinsically disordered and forms amyloid-like fibrils in solution under physiological conditions. The 25 N-terminal resid...

journal_title：Biochemistry

authors： Zhang X,Adda CG,Low A,Zhang J,Zhang W,Sun H,Tu X,Anders RF,Norton RS

更新日期：2012-02-21 00:00:00

abstract：:On the basis of the available high-resolution structures of mandelate racemase (MR) from Pseudomonas putida [Landro, J. A., Gerlt, J. A., Kozarich, J. W., Koo, C. W., Shah, V. J., Kenyon, G. L., Neidhart, D. J., Fujita, J., & Petsko, G. A. (1994) Biochemistry 33, 635-643], Lys 166 and His 297 are positioned appropriat...

journal_title：Biochemistry

authors： Kallarakal AT,Mitra B,Kozarich JW,Gerlt JA,Clifton JG,Petsko GA,Kenyon GL

更新日期：1995-03-07 00:00:00

abstract：:The 24-kDa subunit of mitochondrial NADH-ubiquinone reductase (complex I) is an iron-sulfur protein that is present in the flavoprotein or NADH dehydrogenase II subcomplex. It is a nuclear gene product and is imported into the organelle. A group of human patients with mitochondrial myopathy have been shown to have red...

journal_title：Biochemistry

authors： Pilkington SJ,Walker JE

更新日期：1989-04-18 00:00:00

abstract：:For many different enveloped viruses the crystal structure of the fusion protein core has been established. A striking conservation in the tertiary and quaternary arrangement of these core structures is repeatedly revealed among members of diverse families. It has been proposed that the primary role of the core involv...

journal_title：Biochemistry

authors： Sackett K,Shai Y

更新日期：2002-04-09 00:00:00

abstract：:According to earlier genetic experiments, a region within the N-terminal 50-100 amino acids may be important for the replication function of T antigen, the initiator protein of simian virus 40 (SV40). We have investigated this possibility using the T antigen related D2 protein in several biochemical assay systems. D2 ...

journal_title：Biochemistry

authors： Klausing K,Scheffner M,Scheidtmann KH,Stahl H,Knippers R

更新日期：1989-03-07 00:00:00

abstract：:Transmembrane (TM) alpha-helical peptides with neutral flanking residues such as tryptophan form highly ordered striated domains when incorporated in gel-state 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) bilayers and inspected by atomic force microscopy (AFM) (1). In this study, we analyze the molecular organiz...

journal_title：Biochemistry

authors： Sparr E,Ganchev DN,Snel MM,Ridder AN,Kroon-Batenburg LM,Chupin V,Rijkers DT,Killian JA,de Kruijff B

更新日期：2005-01-11 00:00:00

abstract：:The RecB and RecC subunits of the RecBCD enzyme from Escherichia coli were purified from cells containing plasmids overproducing these proteins [Boehmer, P.E., & Emmerson, P.T. (1991) Gene 102, 1-6]. RecB hydrolyzes ATP in the presence of either single- or double-stranded DNA. RecC stimulates ATP hydrolysis by RecB, p...

journal_title：Biochemistry

authors： Korangy F,Julin DA

更新日期：1993-05-11 00:00:00

abstract：:Cdc42 and Rac are highly homologous members of the Rho family of small G proteins that interact with several downstream effector proteins thereby causing cytoskeletal rearrangements, cell proliferation, and differentiation. While some effectors, such as the tyrosine kinase, ACK, and the scaffold protein, WASP, are uni...

journal_title：Biochemistry

authors： Elliot-Smith AE,Mott HR,Lowe PN,Laue ED,Owen D

更新日期：2005-09-20 00:00:00

abstract：:Curcumin potentiates the phosphorylation-dependent activity of human cystic fibrosis transmembrane conductance regulator (CFTR) in Fe(3+)-dependent and Fe(3+)-independent manners. Although the Fe(3+)-dependent curcumin potentiation results from removal of endogenous inhibitory Fe(3+) at the interface of the regulatory...

journal_title：Biochemistry

authors： Wang G

更新日期：2015-05-12 00:00:00

abstract：:An X-ray structure analysis of a crystal of mutant Asp229----Ala of cyclodextrin glycosyltransferase from Bacillus circulans (Ec 2.4.1.19) that had been shortly exposed to beta-cyclodextrin showed density corresponding to a maltose bound at the catalytic center. The crystal structure was refined to an R-factor of 18.7...

journal_title：Biochemistry

authors： Klein C,Hollender J,Bender H,Schulz GE

更新日期：1992-09-22 00:00:00

abstract：:The complexity of rat liver poly (A)+ messenger RNA (mRNA) has been measured by analysis of the kinetics of hydridization with both complementary DNA (cDNA) and single copy DNA. The complementary DNA-poly(A)+ mRNA hybridization reaction demonstrates the existence of three abundance classes representing 18, 37, and 45%...

journal_title：Biochemistry

authors： Savage MJ,Sala-Trepat JM,Bonner J

更新日期：1978-02-07 00:00:00

abstract：:The fatty acid synthase (FAS) of animal tissue is a dimer of two identical subunits, each with a Mr of 260,000. The subunit is a single multifunctional protein having seven catalytic activities and a site for binding of the prosthetic group 4'-phosphopantetheine. The mRNA coding for the subunit has an estimated size o...

journal_title：Biochemistry

authors： Kasturi R,Chirala S,Pazirandeh M,Wakil SJ

更新日期：1988-10-04 00:00:00

abstract：:The oxidative stress products malondialdehyde and base propenal react with DNA bases forming the adduction products 3-(2'-deoxy-β-D-erythro-pentofuranosyl)pyrimido[1,2-a]purin-10(3H)-one (M1dG) and N(6)-(oxypropenyl)-2'-deoxyadenosine (OPdA). M1dG is mutagenic in vivo and miscodes in vitro, but little work has been do...

journal_title：Biochemistry

authors： Maddukuri L,Shuck SC,Eoff RL,Zhao L,Rizzo CJ,Guengerich FP,Marnett LJ

更新日期：2013-12-03 00:00:00

abstract：:When rabbits are fed a diet supplemented with cholesterol, their plasma cholesterol levels increase markedly, and they develop atherosclerosis. Most of the plasma cholesterol exists as cholesteryl esters in very low density lipoproteins (VLDL) and intermediate-density lipoproteins (IDL). The triglyceride content of th...

journal_title：Biochemistry

authors： Kroon PA,Seidenberg J

更新日期：1982-12-07 00:00:00

abstract：:The complete amino acid sequence of ragweed pollen allergen Ra3 has been determined. The molecule consists of 101 amino acid residues and to date is the only allergen isolated from Ambrosia elatior (short ragweed) which contains carbohydrate. This particular preparation of allergen has a single, unique amino acid sequ...

journal_title：Biochemistry

authors： Klapper DG,Goodfriend L,Capra JD

更新日期：1980-12-09 00:00:00

abstract：:De novo purine biosynthesis proceeds by two divergent paths. In bacteria, yeasts, and plants, 5-aminoimidazole ribonucleotide (AIR) is converted to 4-carboxy-AIR (CAIR) by two enzymes: N(5)-carboxy-AIR (N(5)-CAIR) synthetase (PurK) and N(5)-CAIR mutase (class I PurE). In animals, the conversion of AIR to CAIR requires...

journal_title：Biochemistry

authors： Tranchimand S,Starks CM,Mathews II,Hockings SC,Kappock TJ

更新日期：2011-05-31 00:00:00

abstract：:Analysis of [gamma-32P]ATP in vitro labeled nuclear RNA shows transfer of gamma-32P from ATP to form the 5'-terminal monophosphate for large RNA molecules. This finding is an actively transcribing nuclear system capable of guanylation and methylation reactions indicates that polynucleotide kinase activity in the eukar...

journal_title：Biochemistry

authors： Winicov I

更新日期：1977-09-20 00:00:00

abstract：:Diphtheria toxin (DT) has been studied as a model for understanding active-site structure and function in the ADP-ribosyltransferases. Earlier evidence suggested that histidine-21 of DT is important for the ADP-ribosylation of eukaryotic elongation factor 2 (EF-2). We have generated substitutions of this residue by ca...

journal_title：Biochemistry

authors： Blanke SR,Huang K,Wilson BA,Papini E,Covacci A,Collier RJ

更新日期：1994-05-03 00:00:00

abstract：:During a recent investigation of the persistence of substrate ambiguity in contemporary enzymes, we identified three distinct ambiguous sugar kinases embedded within the modern Escherichia coli genome [Miller, B. G., and Raines, R. T. (2004) Biochemistry 43, 6387-6392]. These catalysts are the YajF, YcfX, and NanK pol...

journal_title：Biochemistry

authors： Miller BG,Raines RT

更新日期：2005-08-16 00:00:00

abstract：:There are at least nine neurodegenerative diseases associated with proteins that contain an unusually expanded polyglutamine domain, the best known of which is Huntington's disease. In all of these diseases, the mutant protein aggregates into neuronal inclusions; it is generally, although not universally, believed tha...

journal_title：Biochemistry

authors： Lee CC,Walters RH,Murphy RM

更新日期：2007-11-06 00:00:00

abstract：:Protein phosphatase 1 (PP1) is an essential and ubiquitous serine/threonine protein phosphatase that is regulated by more than 100 known inhibitor and targeting proteins. It is currently unclear how protein inhibitors distinctly and specifically regulate PP1 to enable rapid responses to cellular alterations. We demons...

journal_title：Biochemistry

authors： Dancheck B,Nairn AC,Peti W

更新日期：2008-11-25 00:00:00

abstract：:In eukaryotes, a major route for ATP-dependent protein breakdown proceeds through covalent intermediates of target proteins destined for degradation and the highly conserved, 76 amino acid protein ubiquitin. In rabbit reticulocytes, it has been shown that hemin effectively inhibits this pathway by blocking the catabol...

journal_title：Biochemistry

authors： Vierstra RD,Sullivan ML

更新日期：1988-05-03 00:00:00