Kinetics and processivity of ATP hydrolysis and DNA unwinding by the RecBC enzyme from Escherichia coli.

abstract：:The effect of RNA secondary structure on the kinetics of DNA synthesis catalyzed by HIV-1 RT was determined using a 66 nucleotide RNA template containing a stable 12 base pair hairpin structure. Prior to reaching the hairpin structure, the primer elongation by RT was fast and the kinetics of polymerization was not aff...

journal_title：Biochemistry

authors： Suo Z,Johnson KA

更新日期：1997-10-14 00:00:00

abstract：:Glutamate racemase, MurI, catalyzes the interconversion of glutamate enantiomers in a cofactor-independent fashion and provides bacteria with a source of D-Glu for use in peptidoglycan biosynthesis. The enzyme uses a "two-base" mechanism involving a deprotonation of the substrate at the alpha-position to form an anion...

journal_title：Biochemistry

authors： Glavas S,Tanner ME

更新日期：2001-05-29 00:00:00

abstract：:Periodate-oxidized tRNA(Phe) (tRNA(oxPhe)) behaves as a specific affinity label of tetrameric Escherichia coli phenylalanyl-tRNA synthetase (PheRS). Reaction of the alpha 2 beta 2 enzyme with tRNA(oxPhe) results in the loss of tRNAPhe aminoacylation activity with covalent attachment of 2 mol of tRNA dialdehyde/mol of ...

journal_title：Biochemistry

authors： Hountondji C,Schmitter JM,Beauvallet C,Blanquet S

更新日期：1987-08-25 00:00:00

abstract：:Bacterioferritins are members of a class of spherical shell-like iron storage proteins that catalyze the oxidation and hydrolysis of iron at specific sites inside the protein shell, resulting in formation of a mineral core of hydrated ferric oxide within the protein cavity. Electrode oximetry/pH stat was used to study...

journal_title：Biochemistry

authors： Yang X,Le Brun NE,Thomson AJ,Moore GR,Chasteen ND

更新日期：2000-04-25 00:00:00

abstract：:Intrastrand cross-links represent the majority of modifications in DNA resulting from interaction with the cancer chemotherapeutic drug cis-diamminedichloroplatinum(II) (cis-DDP). These adducts were recently characterized although several discrepancies remained to be resolved. In these studies, [3H]-cis-dichloro(ethyl...

journal_title：Biochemistry

authors： Eastman A

更新日期：1986-07-01 00:00:00

abstract：:Rhodospirillum rubrum ribulose bisphosphate carboxylase contains two high affinity binding sites for pyridoxal phosphate and two catalytic sites per dimer. However, pyridoxal phosphate binding at only one site is sufficient for inactivation of both catalytic sites. In the presence of 20 mM bicarbonate, 10 mM magnesium...

journal_title：Biochemistry

authors： Whitman WB,Tabita FR

更新日期：1978-04-04 00:00:00

abstract：:The temperature dependence of the packing (order) and fluidity (microviscosity) of rabbit small, intestinal brush border vesicle membranes and of liposomes made from their extracted lipids has been investigated by using a variety of lipid spin probes. The lipids in the brush border membrane are present essentially as ...

journal_title：Biochemistry

authors： Hauser H,Gains N,Semenza G,Spiess M

更新日期：1982-10-26 00:00:00

abstract：:The light chain of activated factor IX (FIXa) is involved in a number of functional properties, including FIXa enzymatic activity. This suggests the existence of a functional link between the FIXa light chain and the catalytic domain. The FIXa structure includes a few putative interactions between EGF2 and the proteas...

journal_title：Biochemistry

authors： Fribourg C,Meijer AB,Mertens K

更新日期：2006-09-05 00:00:00

abstract：:The solution conformation is presented for representatives of each of the major classes of asparaginyl oligosaccharides. In this report the conformation of alpha(1-3)-, alpha(1-2)-, beta(1-2)-, and beta(1-4)-linked units is described. The conformational properties of these glycopeptides were determined by high-resolut...

journal_title：Biochemistry

authors： Brisson JR,Carver JP

更新日期：1983-07-19 00:00:00

abstract：:In aspartate aminotransferase, pyridoxal 5'-phosphate (PLP) forms a Schiff base with the epsilon-amino group of Lys258 (internal aldimine). The internal aldimine has a pKa value of 6.8. Binding of a substrate amino acid to the enzyme yields the Michaelis complex, in which PLP still forms the internal aldimine with Lys...

journal_title：Biochemistry

authors： Hayashi H,Mizuguchi H,Kagamiyama H

更新日期：1998-10-27 00:00:00

abstract：:P16(INK4A) (also known as P16 and MTS1), a protein consisting exclusively of four ankyrin repeats, is recognized as a tumor suppressor mainly because of the prevalence of genetic inactivation of the p16(INK4A) (or CDKN2A) gene in virtually all types of human cancers. However, it has also been shown that an elevated le...

journal_title：Biochemistry

authors： Li J,Poi MJ,Tsai MD

更新日期：2011-06-28 00:00:00

abstract：:Thermodynamic studies of bacteriorhodopsin (BR) have been undertaken in order to investigate the factors that stabilize the structure of a membrane protein. The stability of the native, intact protein was compared to that of protein with retinal removed, and/or cleaved in one or two of the loops connecting the transme...

journal_title：Biochemistry

authors： Kahn TW,Sturtevant JM,Engelman DM

更新日期：1992-09-22 00:00:00

abstract：:The temperature dependence of the hydrogen-tritium and deuterium-hydrogen exchange reactions in poly(DL-alanine) has been reexamined. The results indicate a significant contribution to the observed exchange rates from the water-catalyzed reaction at pD values near pDmin. The activation enthalpy for water-catalyzed deu...

journal_title：Biochemistry

authors： Gregory RB,Crabo L,Percy AJ,Rosenberg A

更新日期：1983-02-15 00:00:00

abstract：:The three-dimensional structure of the fatty-acid-binding protein isolated from the flight muscle of the desert locust Schistocerca gregaria has been solved and refined to a crystallographic R-value of 18.5% for all measured X-ray data from 30.0- to 2.2-A resolution. Crystals employed in the investigation were grown f...

journal_title：Biochemistry

authors： Haunerland NH,Jacobson BL,Wesenberg G,Rayment I,Holden HM

更新日期：1994-10-18 00:00:00

abstract：:The crystal structures of 7,8-dimethylisoalloxazine-10-acetic acid-tryptamine (1:1) tetrahydrate and 7,8-dimethylisoalloxazine-10-acetic acid-tyramine (1:1) tetrahydrate complexes were determined by the X-ray diffraction method, as models for flavin-tryptophan and flavin-tyrosine interactions in flavoproteins. The obs...

journal_title：Biochemistry

authors： Inoue M,Shibata M,Kondo Y,Ishida T

更新日期：1981-05-12 00:00:00

abstract：:NADH oxidase activity has been detected in luminal membrane derived from the proximal tubules of rat kidney (brush border membranes). The cytochromes associated with these membranes [García, M.L., Benavides, J., Valdivieso, F., Mayor, F., & Giménez-Gallego, G. (1978) Biochem. Biophys. Res. Commun. 82, 738-744] were re...

journal_title：Biochemistry

authors： Giménez-Gallego G,Benavides J,García ML,Valdivieso F

更新日期：1980-10-14 00:00:00

abstract：:Brd4 belongs to the BET family. It is a multifunctional protein involved in transcription, replication, the signal transduction pathway, and cell cycle progression. All of these functions are linked to its association with acetylated chromatin. With its tandem bromodomains, Brd4 avidly binds to diacetylated H4-AcK5/K1...

journal_title：Biochemistry

authors： Liu Y,Wang X,Zhang J,Huang H,Ding B,Wu J,Shi Y

更新日期：2008-06-17 00:00:00

abstract：:The major plasma membrane protein of human erythrocytes is the anion transport protein, termed protein 3. We previously reported a variant form of protein 3 that is elongated on the amino-terminal end of the molecule, which is exposed on the cytoplasmic side of the membrane, but otherwise its features are identical wi...

journal_title：Biochemistry

authors： Hsu L,Morrison M

更新日期：1985-06-18 00:00:00

abstract：:Pig kidney general acyl-CoA dehydrogenases forms the blue neutral radical on dithionite or photochemical reduction (Thorpe, C., Matthews, R. G., & Williams, C. H. (1979) Biochemistry 18, 331-337] in accord with its classification as a flavoprotein dehydrogenase. However, dithionite reduction of the enzyme in the prese...

journal_title：Biochemistry

authors： Mizzer JP,Thorpe C

更新日期：1981-08-18 00:00:00

abstract：:Comparative studies on the salt titration and the related kinetics for poly(dG-dC) X poly(dG-dC) in pH 7.0 and 3.8 solutions clearly suggest that base protonation facilitates the kinetics of B-Z interconversion although the midpoint for such a transition in acidic solution (2.0-2.1 M NaCl) is only slightly lower than ...

journal_title：Biochemistry

authors： Chen FM

更新日期：1984-12-04 00:00:00

abstract：:The mechanism by which some hydrophobic molecules such as steroids and free fatty acids (FFA) act as noncompetitive inhibitors of the nicotinic acetylcholine receptor (AChR) is still not known. In the present work, we employ Förster resonance energy transfer (FRET) between the intrinsic fluorescence of membrane-bound ...

journal_title：Biochemistry

authors： Nievas GA,Barrantes FJ,Antollini SS

更新日期：2007-03-20 00:00:00

abstract：:The bacterium Caulobacter crescentus is known to attach irreversibly to underwater surfaces by utilizing an adhesive structure called the holdfast, which exhibits the greatest known adhesive strength of any organism. The very small size of the holdfast (∼400 nm wide and ∼40 nm high) has made direct chemical analysis d...

journal_title：Biochemistry

authors： Nyarko A,Singla S,Barton HA,Dhinojwala A

更新日期：2020-09-22 00:00:00

abstract：:Prokaryotic and eukaryotic cytochrome c oxidases and several bacterial ubiquinol oxidases compose a superfamily of heme-copper oxidases. These enzymes are terminal components of aerobic respiratory chains, the principal energy-generating systems of aerobic organisms. Two such heme-copper oxidases are the aa3-type cyto...

journal_title：Biochemistry

authors： Calhoun MW,Thomas JW,Hill JJ,Hosler JP,Shapleigh JP,Tecklenburg MM,Ferguson-Miller S,Babcock GT,Alben JO,Gennis RB

更新日期：1993-10-12 00:00:00

abstract：:Calmodulin is a ubiquitous calcium dependent protein. In the presence of calcium, calmodulin adopts an altered conformation that leads to the generation of downstream cellular calcium signals. Here we describe the introduction of nitroxide EPR probes into calmodulin by means of site-directed spin labeling. These probe...

journal_title：Biochemistry

authors： Shao J,Cieslak J,Gross A

更新日期：2009-01-27 00:00:00

abstract：:Tumor suppressor Nore1, its acronym coming from novel Ras effector, is one of the 10 members of the Rassf (Ras association domain family) protein family that have been identified. It is expressed as two mRNA splice variants, Nore1A and a shorter isoform, Nore1B. It forms homo- and heterocomplexes through its C-termina...

journal_title：Biochemistry

authors： Makbul C,Constantinescu Aruxandei D,Hofmann E,Schwarz D,Wolf E,Herrmann C

更新日期：2013-02-12 00:00:00

abstract：:Protein kinase C-related kinases (PRKs) are serine/threonine kinases that are members of the protein kinase C superfamily and can be activated by binding to members of the Rho family of small G proteins via a Rho binding motif known as an HR1 domain. The PRKs contain three tandem HR1 domains at their N-termini. The st...

journal_title：Biochemistry

authors： Hutchinson CL,Lowe PN,McLaughlin SH,Mott HR,Owen D

更新日期：2011-04-12 00:00:00

abstract：:We have reexamined the binding properties of the antitumor drug daunomycin using double-helical oligonucleotides 16 base pairs long that were designed to contain preferred binding sites for the drug. The preferred sites are contained in a six base pair core which is flanked on the 5' and 3' ends by tracts of adenines....

journal_title：Biochemistry

authors： Roche CJ,Thomson JA,Crothers DM

更新日期：1994-02-01 00:00:00

abstract：:In clostridial-type ferredoxins, each of the two [4Fe-4S]2+/+ clusters receives three of its four ligands from a CysXXCysXXCys motif. Azotobacter vinelandii ferredoxin I (AvFdI) is a seven-iron ferredoxin that contains one [4Fe-4S]2+/+ cluster and one [3Fe-4S]+/0 cluster. During the evolution of the 7Fe azotobacter-ty...

journal_title：Biochemistry

authors： Kemper MA,Gao-Sheridan HS,Shen B,Duff JL,Tilley GJ,Armstrong FA,Burgess BK

更新日期：1998-09-15 00:00:00

abstract：:The Escherichia coli repressor of biotin biosynthesis is both a biotin ligase and the repressor of transcriptional initiation at the biotin biosynthetic operon. The small molecule, biotinyl-5'-adenylate (bio-5'-AMP), is the intermediate in the biotin ligation reaction and the positive allosteric effector for sequence-...

journal_title：Biochemistry

authors： Xu Y,Beckett D

更新日期：1994-06-14 00:00:00

abstract：:Cooperative binding of the bacteriophage lambda cI repressor dimer to specific sites of the phage operators OR and OL controls the developmental state of the phage. Cooperativity has long been thought to be mediated by self-assembly of repressor dimers to form tetramers which can bind simultaneously to adjacent operat...

journal_title：Biochemistry

authors： Rusinova E,Ross JB,Laue TM,Sowers LC,Senear DF

更新日期：1997-10-21 00:00:00