RNA phosphorylation: a polynucleotide kinase function in mouse L cell nuclei.

abstract：:Muscarinic cholinergic receptors can appear to be more numerous when labeled by [(3)H]quinuclidinylbenzilate (QNB) than by N-[(3)H]methylscopolamine (NMS). The nature of the implied heterogeneity has been studied with M(2) receptors in detergent-solubilized extracts of porcine atria. The relative capacity for [(3)H]NM...

journal_title：Biochemistry

authors： Park PS,Sum CS,Pawagi AB,Wells JW

更新日期：2002-04-30 00:00:00

abstract：:Translocation of DNA during in vitro DNA synthesis on nuclear matrix bound replicational assemblies from regenerating rat liver was determined by measuring the processivity (average number of nucleotides added following one productive binding event of the polymerase to the DNA template) of nuclear matrix bound DNA pol...

journal_title：Biochemistry

authors： Tubo RA,Martelli AM,Berezney R

更新日期：1987-09-08 00:00:00

abstract：:The kinetics of human polymerase beta (pol beta) binding to the single-stranded DNA, in the (pol beta)(16) and (pol beta)(5) binding modes, that differ in the number of occluded nucleotide residues in the protein-DNA complexes, have been examined, using the fluorescence stopped-flow technique. This is the first determ...

journal_title：Biochemistry

authors： Rajendran S,Jezewska MJ,Bujalowski W

更新日期：2001-10-02 00:00:00

abstract：:We have determined the mechanism of DNA-dependent protein kinase (DNA-PK) inhibition by cis-diamminedichloroplatinum(II)-(cisplatin-) damaged DNA. We previously have demonstrated that Ku, the DNA binding subunit of DNA-PK, is capable of binding to DNA duplexes globally damaged with cisplatin but was unable to stimulat...

journal_title：Biochemistry

authors： Turchi JJ,Patrick SM,Henkels KM

更新日期：1997-06-17 00:00:00

abstract：:Reaction center (RC) complexes isolated from a Zn-bacteriochlorophyll (BChl) a-containing purple bacterium, Acidiphilium rubrum, were characterized by absorption, circular dichroism, and magnetic circular dichroism (MCD) spectroscopy. The oxidized-minus-reduced difference spectra indicated that, in this RC, the Zn-BCh...

journal_title：Biochemistry

authors： Mimuro M,Kobayashi M,Shimada K,Uezono K,Nozawa T

更新日期：2000-04-11 00:00:00

abstract：:The deposition of fibrils and amorphous aggregates of transthyretin (TTR) in patient tissues is a hallmark of TTR amyloid disease, but the molecular details of amyloidogenesis are poorly understood. Tetramer dissociation is typically rate-limiting for TTR amyloid fibril formation, so we have used a monomeric variant o...

journal_title：Biochemistry

authors： Hurshman AR,White JT,Powers ET,Kelly JW

更新日期：2004-06-15 00:00:00

abstract：:Difference Fourier transform infrared spectra of lumirhodopsin, metarhodopsin I, and metarhodopsin II versus rhodopsin were recorded with hydrated films of bovine rod outer segments at 200, 240, and 270 K. In the region between 3700 and 3450 cm-1, the O-H stretching vibrational bands of water were identified by H(2)18...

journal_title：Biochemistry

authors： Maeda A,Ohkita YJ,Sasaki J,Shichida Y,Yoshizawa T

更新日期：1993-11-16 00:00:00

abstract：:Phosphoenolthiopyruvate, the analogue of phosphoenolpyruvate in which the bridging oxygen of the phosphate ester is replaced by sulfur, has been synthesized from methyl acrylate and dimethyl (chlorothio)phosphonate. The compound is a substrate for alkaline phosphatase, pyruvate kinase, enolase, and phosphoenolpyruvate...

journal_title：Biochemistry

authors： Sikkema KD,O'Leary MH

更新日期：1988-02-23 00:00:00

abstract：:The effect of Mg2+ on the disposition of myosin cross-bridges was studied on myofibrils and synthetic myosin and rod filaments by employing chymotryptic digestion and chemical cross-linking methods. In the presence of low Mg2+ concentrations (0.1 mM), the proteolytic susceptibility at the heavy meromyosin/light meromy...

journal_title：Biochemistry

authors： Reisler E,Liu J,Cheung P

更新日期：1983-10-11 00:00:00

abstract：:The interaction of novel, tetrafunctional bisplatinum compounds with DNA was investigated. These compounds have bis(vicinal 1,2-diamines) as ligand. The reactions' efficiency, types of cross-links, alterations of the global DNA structure, and sequence selectivity differ significantly from the corresponding features of...

journal_title：Biochemistry

authors： Büning H,Altman J,Beck W,Zorbas H

更新日期：1997-09-23 00:00:00

abstract：:Bacillus subtilis CsoR (Bsu CsoR) is a copper-sensing transcriptional repressor that regulates the expression of the copZA operon encoding a copper chaperone and a Cu efflux P-type ATPase, respectively. Bsu CsoR is a homologue of Mycobacterium tuberculosis CsoR (Mtb CsoR), representative of a large Cu(I)-sensing regul...

journal_title：Biochemistry

authors： Ma Z,Cowart DM,Scott RA,Giedroc DP

更新日期：2009-04-21 00:00:00

abstract：:The essential active site Fe3+ of protocatechuate 3,4-dioxygenase [3, 4-PCD, subunit structure (alphabetaFe3+)12] is bound by axial ligands, Tyr447 (147beta) and His462 (162beta), and equatorial ligands, Tyr408 (108beta), His460 (160beta), and a solvent OH- (Wat827). Recent X-ray crystallographic studies have shown th...

journal_title：Biochemistry

authors： Frazee RW,Orville AM,Dolbeare KB,Yu H,Ohlendorf DH,Lipscomb JD

更新日期：1998-02-24 00:00:00

abstract：:To probe the motional and conformational properties of the choline head group of 1,2-dipalmitoyl-sn-glycero-3-thiophosphocholine (DPPsC), the Rp, Sp, and Rp + Sp isomers of [alpha-D2]DPPsC, [beta-D2]DPPsC, and [delta-D9]DPPsC in the subgel, gel, and liquid crystalline phases were investigated with deuterium NMR, and t...

journal_title：Biochemistry

authors： Loffredo WM,Jiang RT,Tsai MD

更新日期：1990-12-11 00:00:00

abstract：:The proximity of skeletal myosin subfragment-1 (S1) to actin, and its orientation with respect to thin filaments of single muscle fibers, were compared in the presence and in the absence of ADP. The proximity was assessed by the efficiency of carbodiimide-induced cross-linking and the orientation by polarization of fl...

journal_title：Biochemistry

authors： Andreev OA,Ushakov DS,Borejdo J

更新日期：1998-12-22 00:00:00

abstract：:The calcium binding protein alpha-lactalbumin folds via a molten globule intermediate. Calcium does not bind strongly to the unfolded protein or the molten globule, but does bind to the transition state between the molten globule and the native protein. Of interest are the structures formed in the transition state tha...

journal_title：Biochemistry

authors： Kuhlman B,Boice JA,Wu WJ,Fairman R,Raleigh DP

更新日期：1997-04-15 00:00:00

abstract：:Class II MHC glycoproteins bind short (7-25 amino acid) peptides in an extended type II polyproline-like conformation and present them for immune recognition. Because empty MHC is unstable, measurement of the rate of the second-order reaction between peptide and MHC is challenging. In this report, we use dissociation ...

journal_title：Biochemistry

authors： Kasson PM,Rabinowitz JD,Schmitt L,Davis MM,McConnell HM

更新日期：2000-02-08 00:00:00

abstract：:Base excision repair (BER) is the major corrective pathway for most spontaneous, oxidative, and alkylation DNA base and sugar damage. X-ray cross-complementing 1 (XRCC1) has been suggested to function at nearly every step of this repair process, primarily through direct protein-protein interactions. Using whole cell e...

journal_title：Biochemistry

authors： Wong HK,Kim D,Hogue BA,McNeill DR,Wilson DM 3rd

更新日期：2005-11-01 00:00:00

abstract：:Binding of 125I-labeled human alpha-thrombin to endothelial cells derived from bovine corneas was studied in tissue culture. Specific and saturable binding to the cell surface occurred at 37 degrees C but to a much smaller extent at 4 degrees C. Binding of [125I]thrombin to a specific site on these cells with formatio...

journal_title：Biochemistry

authors： Isaacs JD,Savion N,Gospodarowicz D,Fenton JW 2nd,Shuman MA

更新日期：1981-01-20 00:00:00

abstract：:A novel cationic lipid was separated from bovine brain white matter by a series of chromatographies on carboxymethyl-Sephadex and silica gel in chloroform and methanol. Its structure was identified unambiguously as de-N-acetyllactotriaosylceramide (deNAcLc(3)Cer) by mass spectrometry and (1)H NMR. The natural occurren...

journal_title：Biochemistry

authors： Hikita T,Tadano-Aritomi K,Iida-Tanaka N,Ishizuka I,Hakomori S

更新日期：2005-07-12 00:00:00

abstract：:Low-temperature electronic absorption and room temperature circular dichroism (CD) and magnetic circular dichroism (MCD) spectra are reported for ascorbate oxidase. Bands attributable to d-d electronic transitions in the type 1 (blue) coppers (2B2 ground state) have been observed at 5800, 10000, and 12000 cm-1 (1725, ...

journal_title：Biochemistry

authors： Dooley DM,Dawson JH,Stephens PJ,Gray HB

更新日期：1981-03-31 00:00:00

abstract：:Isolated alpha and beta chains from Xenopus laevis hemoglobin have been purified. The isolation procedure yields native alpha chains whose functional behavior has been characterized and compared with that of human alpha chains. Isolated beta chains in the presence of oxygen are characterized by low stability, and henc...

journal_title：Biochemistry

authors： Condò SG,Giardina B,Bellelli A,Brunori M

更新日期：1987-10-20 00:00:00

abstract：:Lamin A protein, encoded by the LMNA gene, belongs to the type V intermediate filament protein family and is a major nuclear protein component of higher metazoan organisms, including humans. Lamin A along with B-type lamins impart structural rigidity to the nucleus by forming a lamina that is closely apposed to the in...

journal_title：Biochemistry

authors： Bhattacharjee P,Banerjee A,Banerjee A,Dasgupta D,Sengupta K

更新日期：2013-06-18 00:00:00

abstract：:The cytological architecture of the synaptonemal complex (SC), a meiosis-specific proteinaceous structure, is evolutionarily conserved among eukaryotes. However, little is known about the biochemical properties of SC components or the mechanisms underlying their roles in meiotic chromosome synapsis and recombination. ...

journal_title：Biochemistry

authors： Khan K,Madhavan TP,Kshirsagar R,Boosi KN,Sadhale P,Muniyappa K

更新日期：2013-08-06 00:00:00

abstract：:Several different monoclonal antibodies (mAbs) have been actively developed in the field of Alzheimer's disease (AD) for basic science and clinical applications; however, the binding kinetics of many of the mAbs with the beta-amyloid peptides (Abeta) are poorly understood. A panel of mAbs with different Abeta recognit...

journal_title：Biochemistry

authors： Ramakrishnan M,Kandimalla KK,Wengenack TM,Howell KG,Poduslo JF

更新日期：2009-11-03 00:00:00

abstract：:Heme oxygenase (HO), from the pathogenic bacterium N. meningitidis(NmHO), which secures host iron, shares many properties with mammalian HOs but also exhibits some key differences. The crystal structure appears more compact, and the crystal-undetected C-terminus interacts with substrate in solution. The unique nature ...

journal_title：Biochemistry

authors： Peng D,Satterlee JD,Ma LH,Dallas JL,Smith KM,Zhang X,Sato M,La Mar GN

更新日期：2011-10-18 00:00:00

abstract：:C-type natriuretic peptide (CNP) binds the guanylyl cyclase-linked natriuretic peptide receptor B (NPR-B) and stimulates marked elevations of the intracellular signaling molecule, cGMP. Here, the essential role of phosphorylation in the hormonal activation and deactivation of this receptor is described. Exposure of NI...

journal_title：Biochemistry

authors： Potter LR

更新日期：1998-02-24 00:00:00

abstract：:The N-terminus of the major polypeptide component of the light-harvesting complex (LHC) from the brown alga Laminaria saccharina is blocked. Two partial sequences, one near the N-terminus and the other near the C-terminus, have been obtained by chemical cleavage with acetic acid and N-chlorosuccinimide. Four peptides ...

journal_title：Biochemistry

authors： Douady D,Rousseau B,Caron L

更新日期：1994-03-22 00:00:00

abstract：:Structures of several voltage-gated sodium (NaV) channels from bacteria have been determined recently, but the same feat might not be achieved for the mammalian counterparts in the near future. Thus, at present, computational studies of the mammalian NaV channels have to be performed using homology models based on the...

journal_title：Biochemistry

authors： Patel D,Mahdavi S,Kuyucak S

更新日期：2016-03-29 00:00:00

abstract：:2,3-Butanedione monoxime (BDM) reversibly inhibits force production in muscle. At least part of its action appears to be directly on the contractile apparatus. To understand better its mechanism of action, we studied the effect of BDM on the steps of myosin subfragment 1 Mg(2+)-ATPase in 0.1 M potassium acetate, pH 7....

journal_title：Biochemistry

authors： Herrmann C,Wray J,Travers F,Barman T

更新日期：1992-12-08 00:00:00

abstract：:The zinc-dependent metallo-beta-lactamases are a group of bacterial enzymes that pose a threat to the future efficacy of present-day antibiotics. Their mechanism is poorly understood, and there are no clinically useful inhibitors. While most members of the group contain two tightly bound zinc ions in their active site...

journal_title：Biochemistry

authors： Davies AM,Rasia RM,Vila AJ,Sutton BJ,Fabiane SM

更新日期：2005-03-29 00:00:00