Mitochondrial NADH-ubiquinone reductase: complementary DNA sequences of import precursors of the bovine and human 24-kDa subunit.

abstract：:Neuropeptide Y (NPY) and the pancreatic polypeptide (PP) are members of the neuropeptide Y family of hormones. They bind to the Y receptors with very different affinities: Whereas PP is highly selective for the Y(4) receptor, NPY displays highest affinites for Y(1), Y(2), and Y(5) receptor subtypes. Introducing the NP...

journal_title：Biochemistry

authors： Lerch M,Kamimori H,Folkers G,Aguilar MI,Beck-Sickinger AG,Zerbe O

更新日期：2005-06-28 00:00:00

abstract：:Recombinant human brain calbindin D(28K) (rHCaBP), human Cu,Zn-superoxide dismutase (HCuZnSOD), rabbit muscle glyceraldehyde-3-phosphate dehydrogenase (GAPDH), and bovine serum albumin (BSA) were found to be S-glutathiolated in decomposed S-nitrosoglutathione (GSNO) solutions. Tryptic or Glu-C digestion and MALDI-TOF ...

journal_title：Biochemistry

authors： Tao L,English AM

更新日期：2004-04-06 00:00:00

abstract：:Sulfenic acid is formed upon oxidation of thiols and is a central intermediate in the redox modulation of an increasing number of proteins. Methods for quantifying or even detecting sulfenic acid are scarce. Herein, the reagent 7-chloro-4-nitrobenz-2-oxa-1,3-diazole was determined not to be suitable as a chromophoric ...

journal_title：Biochemistry

authors： Turell L,Botti H,Carballal S,Ferrer-Sueta G,Souza JM,Durán R,Freeman BA,Radi R,Alvarez B

更新日期：2008-01-08 00:00:00

abstract：:The electrons extracted from the CaMn(4) cluster during water oxidation in photosystem II are transferred to P(680)(+) via the redox-active tyrosine D1-Tyr161 (Y(Z)). Upon Y(Z) oxidation a proton moves in a hydrogen bond toward D1-His190 (His(Z)). The deprotonation and reprotonation mechanism of Y(Z)-OH/Y(Z)-O is of k...

journal_title：Biochemistry

authors： Sjöholm J,Havelius KG,Mamedov F,Styring S

更新日期：2010-11-16 00:00:00

abstract：:Colchicine induces a weak assembly-independent GTPase activity in calf brain tubulin [David-Pfeuty, T., Erickson, H. P., & Pantaloni, D. (1977) Proc. Natl. Acad. Sci. U.S.A. 74, 5372-5376; Andreu, J. M., & Timasheff, S. N. (1981) Arch. Biochem. Biophys. 211, 151-157]. Kinetic analysis shows a turnover number of 2 x 10...

journal_title：Biochemistry

authors： Perez-Ramirez B,Shearwin KE,Timasheff SN

更新日期：1994-05-24 00:00:00

abstract：:The specific rate of superoxide (O2(•-)) production in the purified active crystallizable cytochrome b6f complex, normalized to the rate of electron transport, has been found to be more than an order of magnitude greater than that measured in isolated yeast respiratory bc1 complex. The biochemical and structural basis...

journal_title：Biochemistry

authors： Baniulis D,Hasan SS,Stofleth JT,Cramer WA

更新日期：2013-12-17 00:00:00

abstract：:13C NMR spectroscopy has been used to study the amino group environments and metal binding properties of 13C reductively methylated bovine alpha-lactalbumin. Bovine alpha-lactalbumin is a Ca2+ metalloprotein containing 12 lysyl amino groups and a free amino terminus. All 13 amino groups can be 13C-dimethylated without...

journal_title：Biochemistry

authors： Gerken TA

更新日期：1984-09-25 00:00:00

abstract：:A combined N- and C-terminal truncation variant of human apolipoprotein A-I (apoA-I) was designed, expressed in Escherichia coli, isolated, and characterized. Hydrodynamic experiments yielded a weight average molecular weight of 34000, indicating apoA-I-(44-186) exists in solution predominantly as a dimer. An axial ra...

journal_title：Biochemistry

authors： Beckstead JA,Block BL,Bielicki JK,Kay CM,Oda MN,Ryan RO

更新日期：2005-03-22 00:00:00

abstract：:Mitomycins make up a group of antitumor natural products that are biosynthesized from aminohydroxybenzoic acid (AHBA) and N-acetylglucosamine (GlcNAc). While the biosynthetic gene cluster was reported two decades ago, the mechanism by which the two building blocks, AHBA and GlcNAc, are coupled during biosynthesis rema...

journal_title：Biochemistry

authors： Nguyen HP,Yokoyama K

更新日期：2019-06-25 00:00:00

abstract：:Factor VIII (FVIII) is the nonproteolytic cofactor for FIXa in the tenase complex of blood coagulation. FVIII is proteolytically activated by thrombin and FXa in vitro to form a heterotrimer with full procoagulant activity. Activated protein C inactivates thrombin-activated FVIII through cleavage adjacent to position ...

journal_title：Biochemistry

authors： O'Brien DP,Johnson D,Byfield P,Tuddenham EG

更新日期：1992-03-17 00:00:00

abstract：:The method of transient electric birefringence has been applied to study the conformation of protein-sodium dodecyl sulfate complexes. A model of a deformable prolate ellipsoid has been proposed for the protein-dodecyl sulfate complex. This model is compared to the models proposed by J. A. Reynolds and C. Tanford (197...

journal_title：Biochemistry

authors： Wright AK,Thompson MR,Miller RL

更新日期：1975-07-15 00:00:00

abstract：:The nuclear tRNA 3' processing activity from wheat has been characterized and partially purified. Several characteristics of the wheat nuclear 3' processing enzyme now allow this activity to be distinguished from its mitochondrial counterpart. The nuclear enzyme is an endonuclease, which we termed nuclear RNase Z. The...

journal_title：Biochemistry

authors： Mayer M,Schiffer S,Marchfelder A

更新日期：2000-02-29 00:00:00

abstract：:We consider the problem of color regulation in visual pigments for both bovine rhodopsin (lambda max = 500 nm) and octopus rhodopsin (lambda max = 475 nm). Both pigments have 11-cis-retinal (lambda max = 379 nm, in ethanol) as their chromophore. These rhodopsins were bleached in their native membranes, and the opsins ...

journal_title：Biochemistry

authors： Koutalos Y,Ebrey TG,Tsuda M,Odashima K,Lien T,Park MH,Shimizu N,Derguini F,Nakanishi K,Gilson HR

更新日期：1989-03-21 00:00:00

abstract：:Over 70 transthyretin (TTR) mutations facilitate amyloidosis in tissues other than the central nervous system (CNS). In contrast, the D18G TTR mutation in individuals of Hungarian descent leads to CNS amyloidosis. D18G forms inclusion bodies in Escherichia coli, unlike the other disease-associated TTR variants overexp...

journal_title：Biochemistry

authors： Hammarström P,Sekijima Y,White JT,Wiseman RL,Lim A,Costello CE,Altland K,Garzuly F,Budka H,Kelly JW

更新日期：2003-06-10 00:00:00

abstract：:A series of peptide fragments of sperm whale myoglobin, corresponding to segments of the region between the G- and H-helices of the protein, have been synthesized and their conformational preferences investigated using circular dichroism and nuclear magnetic resonance spectroscopy in aqueous solution and in solvent mi...

journal_title：Biochemistry

authors： Shin HC,Merutka G,Waltho JP,Wright PE,Dyson HJ

更新日期：1993-06-29 00:00:00

abstract：:Orthophosphate and phosphate derivatives including pyrophosphate, hexametaphosphate, ATP, ADP, and inositol hexaphosphate enhance the extent of DNA degradation by iron(II) bleomycin. These phosphate-containing compounds increase both the release of free nucleic base and that of base propenals which are DNA cleavage pr...

journal_title：Biochemistry

authors： Burger RM,Horwitz SB,Peisach J

更新日期：1985-07-02 00:00:00

abstract：:To study whether protein kinase C (PKC) isoforms can interact with protein-tyrosine-phosphatases (PTPs) which are connected to the insulin signaling pathway, we co-overexpressed PKC isoforms together with insulin receptor, docking proteins, and the PTPs SHP1 and SHP2 in human embryonic kidney (HEK) 293 cells. After ph...

journal_title：Biochemistry

authors： Strack V,Krützfeldt J,Kellerer M,Ullrich A,Lammers R,Häring HU

更新日期：2002-01-15 00:00:00

abstract：:Complementary DNA clones coding for the human secreted carbonic anhydrase isozyme (CA VI) have been isolated and their nucleotide sequences determined. These clones identify a 1.45-kb mRNA that is present in high levels in parotid submandibular salivary glands but absent in other tissues such as the sublingual gland, ...

journal_title：Biochemistry

authors： Aldred P,Fu P,Barrett G,Penschow JD,Wright RD,Coghlan JP,Fernley RT

更新日期：1991-01-15 00:00:00

abstract：:Minor leucine tRNA species, tRNA(Leu)4 and tRNA(Leu)5, from Escherichia coli B have been reported to recognize leucine codons UUA and UUG [Goldman, E., Holmes, W. M., and Hatfield, G. W. (1979) J. Mol. Biol. 129, 567-585]. In the present study, these two tRNA(Leu) species were purified from E. coli A19, and the nucleo...

journal_title：Biochemistry

authors： Horie N,Yamaizumi Z,Kuchino Y,Takai K,Goldman E,Miyazawa T,Nishimura S,Yokoyama S

更新日期：1999-01-05 00:00:00

abstract：:Prolyl endopeptidase (Prep) is a member of the prolyl peptidase family and is of interest because of its unique biochemistry and connections to cognitive function. Using an unbiased mass spectrometry (MS)-based peptidomics platform, we identified Prep-regulated peptides in the central nervous system (CNS) of mice by m...

journal_title：Biochemistry

authors： Nolte WM,Tagore DM,Lane WS,Saghatelian A

更新日期：2009-12-22 00:00:00

abstract：:The proprotein convertase furin is a highly specific serine protease modifying and thereby activating proteins in the secretory pathway by proteolytic cleavage. Its substrates are involved in many diseases, including cancer and infections caused by bacteria and viruses. Understanding furin's substrate specificity is c...

journal_title：Biochemistry

authors： Dahms SO,Hardes K,Steinmetzer T,Than ME

更新日期：2018-02-13 00:00:00

abstract：:The thermal denaturation of caricain (the most alkaline of papain-related proteinases) was studied in acid media. Under all conditions tested, caricain denatured irreversibly following a single first-order reaction that involves simultaneous loss of secondary and tertiary structures. Besides, variation of the rate con...

journal_title：Biochemistry

authors： López-Arenas L,Solís-Mendiola S,Hernández-Arana A

更新日期：1999-11-30 00:00:00

abstract：:In affinity selection, calcineurin selects from a population of randomly modified calmodulins those species with which it prefers to interact. The method shows that acetylation of lysines affects calmodulin so as to interfere with its ability to interact with calcineurin. Monoacetylation of any lysine of calmodulin re...

journal_title：Biochemistry

authors： Manalan AS,Klee CB

更新日期：1987-03-10 00:00:00

abstract：:DADH catalyzes the flavin-dependent oxidative deamination of d-amino acids to the corresponding α-keto acids and ammonia. Here we report the first X-ray crystal structures of DADH at 1.06 Å resolution and its complexes with iminoarginine (DADH(red)/iminoarginine) and iminohistidine (DADH(red)/iminohistidine) at 1.30 Å...

journal_title：Biochemistry

authors： Fu G,Yuan H,Li C,Lu CD,Gadda G,Weber IT

更新日期：2010-10-05 00:00:00

abstract：:Oligodeoxyribonucleotides 8-12 nucleotides in length whose sequences are complementary to the 5' end, the initiation codon regions, or the coding regions of rabbit globin mRNA were tested for their ability to inhibit translation in a rabbit reticulocyte lysate and in a wheat germ extract. The oligomers interact specif...

journal_title：Biochemistry

authors： Blake KR,Murakami A,Miller PS

更新日期：1985-10-22 00:00:00

abstract：:Aminoacyl-tRNA synthetases contain one or three Mg(2+) ions in their catalytic sites. In addition to their role in ATP binding, these ions are presumed to play a role in catalysis by increasing the electropositivity of the alpha-phosphate and stabilizing the pentavalent transition state. In the class II aaRS, two high...

journal_title：Biochemistry

authors： Ador L,Jaeger S,Geslain R,Martin F,Cavarelli J,Eriani G

更新日期：2004-06-08 00:00:00

abstract：:The catalytic (C) subunit of cAMP-dependent protein kinase interacts with two classes of inhibitors. The regulatory (R) subunits, types I and II, associate to form an inactive holoenzyme complex that is activated in response to cAMP. The C-subunit is also inhibited by small heat-stable protein kinase inhibitors (PKI's...

journal_title：Biochemistry

authors： Herberg FW,Taylor SS

更新日期：1993-12-21 00:00:00

abstract：:The photoaffinity reagent 6-azido-L-tryptophan was synthesized by chemical methods. It binds reversibly in the dark to the alpha 2 beta 2 complex of tryptophan synthase of Escherichia coli and forms a quinonoid intermediate with enzyme-bound pyridoxal phosphate (lambda max = 476 nm). The absorbance of this chromophore...

journal_title：Biochemistry

authors： Miles EW,Phillips RS

更新日期：1985-08-13 00:00:00

abstract：:Two transcription factors, CRP and CytR, mediate positive and negative control of nine cistrons involved in nucleoside catabolism and recycling in Escherichia coli. The ability of multiple transcription factors to combine in different ways to confer differential gene regulation is of significant interest in both proka...

journal_title：Biochemistry

authors： Holt AK,Senear DF

更新日期：2010-01-26 00:00:00

abstract：:The collagens synthesized by Chinese hamster ovary cells have been isolated and characterized. Although these cells produce very small amounts of collagen, at least five distinct collagenous chains could be identified from radiolabeled media and cell extracts after limited pepsin digestion. Two chains were characteriz...

journal_title：Biochemistry

authors： Limeback H,Sodek J,Aubin J

更新日期：1982-09-14 00:00:00