Estimating the degree of expansion in the transition state for protein unfolding: analysis of the pH dependence of the rate constant for caricain denaturation.

abstract：:Experiments were performed to elucidate the mechanism of hydrocarbon formation in microsomal preparations from the house fly, Musca domestica. Antibody to both house fly cytochrome P450 reductase and a purified cytochrome P450 (CYP6A1) from the house fly inhibited (Z)-9-tricosene (Z9-23:Hy) formation from [15,16-3H]-(...

journal_title：Biochemistry

authors： Reed JR,Quilici DR,Blomquist GJ,Reitz RC

更新日期：1995-12-12 00:00:00

abstract：:We have sought to determine the role of histone H2A phosphorylation in chromatin by examining the distribution of the phosphorylated and unphosphorylated forms of this core histone within the nuclei of mouse and human cells. At any time, only about 15% of the H2A of whole chromatin is in the phosphorylated form, and i...

journal_title：Biochemistry

authors： Prentice DA,Loechel SC,Kitos PA

更新日期：1982-05-11 00:00:00

abstract：:Factor X (FX) is one of the major players in the blood coagulation cascade. Upon activation to FXa, it converts prothrombin to thrombin, which in turn converts fibrinogen into fibrin (blood clots). FXa deficiency causes hemostasis defects, such as intracranial bleeding, hemathrosis, and gastrointestinal blood loss. He...

journal_title：Biochemistry

authors： Abdel-Azeim S,Oliva R,Chermak E,De Cristofaro R,Cavallo L

更新日期：2014-11-11 00:00:00

abstract：:We have performed comparative studies of the neutral glycosphingolipids synthesized by three human myeloid leukemia cell lines, K562, KG1, and HL-60, which were metabolically labeled with [14C]galactose, to evaluate changes in neutral glycosphingolipid synthesis with myeloid cell differentiation. Individual neutral gl...

journal_title：Biochemistry

authors： Buehler J,Qwan E,DeGregorio MW,Macher BA

更新日期：1985-11-19 00:00:00

abstract：:Formylglycinamide ribonucleotide amidotransferase (FGAR-AT) catalyzes the ATP- and glutamine-dependent formation of formylglycinamidine ribonucleotide, ADP, P(i), and glutamate in the fourth step of de novo purine biosynthesis. Like all amidotransferases (ATs), FGAR-AT is proposed to channel ammonia between a glutamin...

journal_title：Biochemistry

authors： Hoskins AA,Anand R,Ealick SE,Stubbe J

更新日期：2004-08-17 00:00:00

abstract：:Choline oxidase catalyzes the oxidation of choline to glycine betaine through a two-step, four-electron reaction with betaine aldehyde as an intermediate. Oxygen is the final electron acceptor. Alcohol oxidation is initiated by the removal of the substrate hydroxyl proton by an unknown active site residue with a pKa v...

journal_title：Biochemistry

authors： Smitherman C,Rungsrisuriyachai K,Germann MW,Gadda G

更新日期：2015-01-20 00:00:00

abstract：:The coexistence effects of multiple kinds of oxidized deoxyribonucleotides were examined using an SV40 origin-dependent in vitro replication system with a HeLa extract. Oxidized dGTP and dATP, 8-hydroxy-2'-deoxyguanosine 5'-triphosphate (8-OH-dGTP) and 2-hydroxy-2'-deoxyadenosine 5'-triphosphate (2-OH-dATP), were used...

journal_title：Biochemistry

authors： Satou K,Kasai H,Masutani C,Hanaoka F,Harashima H,Kamiya H

更新日期：2007-06-05 00:00:00

abstract：:A crude in vitro system which initiates chloroplast DNA synthesis near the D-loop site mapped by electron microscopy [Wu, M., Lou, J. K., Chang, D. Y., Chang, C. H., & Nie, Z. Q. (1986) Proc. Natl. Acad. Sci. U.S.A. 83, 6761-6765] consists of soluble proteins and proteins extracted from purified thylakoid membrane. In...

journal_title：Biochemistry

authors： Wang ZF,Yang J,Nie ZQ,Wu M

更新日期：1991-01-29 00:00:00

abstract：:Human apolipoprotein(a) kringle IV type 10 [apo(a) KIV(10)] contains a strong lysine-binding site (LBS) that mediates the interaction of Lp(a) with biological substrates such as fibrin. Mutations in the KIV(10) LBS have been reported in both the rhesus monkey and chimpanzee, and have been proposed to explain the lack ...

journal_title：Biochemistry

authors： Belczewski AR,Ho J,Taylor FB Jr,Boffa MB,Jia Z,Koschinsky ML

更新日期：2005-01-18 00:00:00

abstract：:The generality of the concept of a rate-limiting step in enzymic reactions recently has been questioned [Northrop, D. B. (1981) Biochemistry 20, 4056-4061] because, in simulated isotopic experiments, alterations of the step identified as rate limiting by current definitions do not consistently affect Vmax in the expec...

journal_title：Biochemistry

authors： Ray WJ Jr

更新日期：1983-09-27 00:00:00

abstract：:Fusion of the human immunodeficiency virus (HIV) with target cells is mediated by the gp41 subunit of the envelope protein. Mutation and deletion studies within the transmembrane domain (TMD) of intact gp41 influenced its fusion activity. In addition, current models suggest that the TMD is in proximity with the fusion...

journal_title：Biochemistry

authors： Reuven EM,Dadon Y,Viard M,Manukovsky N,Blumenthal R,Shai Y

更新日期：2012-04-03 00:00:00

abstract：:M proteins and other members of the M protein family, expressed on the surface of Streptococcus pyogenes, bind host proteins such as immunoglobulins, albumin, and fibrinogen. Protein H and the M1 protein are expressed by adjacent genes and both belong to the M protein family. In this work, the structure and stability ...

journal_title：Biochemistry

authors： Nilson BH,Frick IM,Akesson P,Forsén S,Björck L,Akerström B,Wikström M

更新日期：1995-10-17 00:00:00

abstract：:Recently, it was demonstrated that bifunctional catalase-peroxidases (KatGs) are found not only in archaea and bacteria but also in lower eukaryotes. Structural studies and preliminary biochemical data of the secreted KatG from the rice pathogen Magnaporthe grisea (MagKatG2) suggested both similar and novel features w...

journal_title：Biochemistry

authors： Gasselhuber B,Carpena X,Graf MM,Pirker KF,Nicolussi A,Sündermann A,Hofbauer S,Zamocky M,Furtmüller PG,Jakopitsch C,Oostenbrink C,Fita I,Obinger C

更新日期：2015-09-08 00:00:00

abstract：:The Met16Phe mutant of the type 1 copper protein pseudoazurin (PACu), in which a phenyl ring is introduced close to the imidazole moiety of the His81 ligand, has been characterized. NMR studies indicate that the introduced phenyl ring is parallel to the imidazole group of His81. The mutation has a subtle effect on the...

journal_title：Biochemistry

authors： Yanagisawa S,Sato K,Kikuchi M,Kohzuma T,Dennison C

更新日期：2003-06-10 00:00:00

abstract：:The mechanism of the oxidative decarboxylation reaction catalyzed by the NAD-malic enzyme from Ascaris suum has been examined with several different divalent metal ion activators and dinucleotide substrates. Primary deuterium and tritium isotope effects have been obtained and, in combination with the partitioning rati...

journal_title：Biochemistry

authors： Karsten WE,Gavva SR,Park SH,Cook PF

更新日期：1995-03-14 00:00:00

abstract：:X-ray crystallographic data have implicated Arg-292 as the residue responsible for the preferred side-chain substrate specificity of aspartate aminotransferase. It forms a salt bridge with the beta or gamma carboxylate group of the substrate [Kirsch, J. F., Eichele, G., Ford, G. C., Vincent, M. G., Jansonius, J. N., G...

journal_title：Biochemistry

authors： Cronin CN,Kirsch JF

更新日期：1988-06-14 00:00:00

abstract：:Site-directed mutants of human carbonic anhydrase III were used to examine the role of Thr-199 and its interaction with Phe-198 in the catalyzed hydration of CO2. Threonine-199 is a hydrogen bond acceptor for the zinc-bound water, and Phe-198 forms part of the hydrophobic side of the active-site cavity of carbonic anh...

journal_title：Biochemistry

authors： Chen X,Tu C,LoGrasso PV,Laipis PJ,Silverman DN

更新日期：1993-08-10 00:00:00

abstract：:The conformation of the glycerol group of a number of diacyl and monoacyl (lyso) phospholipids differing in the chemical nature of the head group was studied by 1H high-resolution NMR and X-ray crystallography. The NMR measurements were carried out with solutions or micellar dispersions of the lipids in deuteriated or...

journal_title：Biochemistry

authors： Hauser H,Pascher I,Sundell S

更新日期：1988-12-27 00:00:00

abstract：:Human hemoglobin produced in the Escherichia coli coexpression system of Hernan et al. [(1992) Biochemistry 31, 8619-8628] has been transformed into a functionally homogeneous protein whose properties closely approximate those of normal hemoglobin A. Both of the alpha and beta chains of this hemoglobin contain a valin...

journal_title：Biochemistry

authors： Hui HL,Kavanaugh JS,Doyle ML,Wierzba A,Rogers PH,Arnone A,Holt JM,Ackers GK,Noble RW

更新日期：1999-01-19 00:00:00

abstract：:Beta-amylase (EC 3.2.1.2) is starch-hydrolyzing exo-type enzyme that can catalyze the successive liberation of beta-maltose from the nonreducing ends of alpha-1,4-linked glucopyranosyl polymers. There is a well-known phenomenon called multiple or repetitive attack where the enzyme releases several maltose molecules in...

journal_title：Biochemistry

authors： Ishikawa K,Nakatani H,Katsuya Y,Fukazawa C

更新日期：2007-01-23 00:00:00

abstract：:G-protein-coupled receptors (GPCRs) have evolved as highly specialized cellular machinery that can dictate biological outcomes in response to diverse stimuli. Specifically, they induce multiple pathway responses upon structural perturbations induced at local protein sites. GPCRs utilize a concurrent strategy involving...

journal_title：Biochemistry

authors： Jatana N,Aswin SK,Rathore S,Thukral L

更新日期：2020-01-28 00:00:00

abstract：:Base excision repair (BER) plays a vital role in maintaining genomic integrity in mammalian cells. DNA polymerase λ (Pol λ) is believed to play a backup role to DNA polymerase β (Pol β) in base excision repair. Two oxidized abasic lesions that are produced by a variety of DNA-damaging agents, including several antitum...

journal_title：Biochemistry

authors： Stevens AJ,Guan L,Bebenek K,Kunkel TA,Greenberg MM

更新日期：2013-02-05 00:00:00

abstract：:A variety of chronic inflammatory conditions are associated with an increased risk for the development of cancer. Because of the numerous links between DNA oxidative damage and carcinogenesis, a potential role for leukocyte-generated oxidants in these processes has been suggested. In the present study, we demonstrate ...

journal_title：Biochemistry

authors： Shen Z,Wu W,Hazen SL

更新日期：2000-05-09 00:00:00

abstract：:A solubilized preparation of steroid 5 alpha-reductase (EC 1.3.1.30) from rat liver has been used in studies focused toward an understanding of the kinetic mechanism associated with enzyme catalysis. From the results of analyses with product and dead-end inhibitors, a preferentially ordered binding of substrates and r...

journal_title：Biochemistry

authors： Levy MA,Brandt M,Greway AT

更新日期：1990-03-20 00:00:00

abstract：:We describe a novel, potent peptide substrate mimetic inhibitor of protein kinase B (PKB/Akt). The compound selectively kills prostate cancer cells, in which PKB is highly activated, but not normal cells, or cancer cells in which PKB is not activated. The inhibitor induces apoptosis and inhibits the phosphorylation of...

journal_title：Biochemistry

authors： Litman P,Ohne O,Ben-Yaakov S,Shemesh-Darvish L,Yechezkel T,Salitra Y,Rubnov S,Cohen I,Senderowitz H,Kidron D,Livnah O,Levitzki A,Livnah N

更新日期：2007-04-24 00:00:00

abstract：:Alpha2u-globulin is a male rat liver protein under multihormonal control which represents approximately 1% of hepatic protein synthesis. We have measured the rate of transcription of the alpha2u-globulin gene using a nuclear cell-free transcriptional system with mercurated CTP as substrate for the endogenous RNA polym...

journal_title：Biochemistry

authors： Chan KM,Kurtz DT,Feigelson P

更新日期：1978-07-25 00:00:00

abstract：:Proteorhodopsins are a recently discovered class of microbial rhodopsins, ubiquitous in marine bacteria. Over 4000 variants have thus far been discovered, distributed throughout the oceans of the world. Most variants fall into one of two major groups, green- or blue-absorbing proteorhodopsin (GPR and BPR, respectively...

journal_title：Biochemistry

authors： Kralj JM,Bergo VB,Amsden JJ,Spudich EN,Spudich JL,Rothschild KJ

更新日期：2008-03-18 00:00:00

abstract：:Significant concentrations (1-10 mM) of nickel(II) were found in solution after incubation of the potent carcinogen nickel subsulfide in 0.05 M Tris-HCl, pH 7.4, solutions containing DNA, rat liver microsomes, and NADPH. The presence of NADPH decreased the rate of solubilization of nickel subsulfide. The solubilized n...

journal_title：Biochemistry

authors： Lee JE,Ciccarelli RB,Jennette KW

更新日期：1982-02-16 00:00:00

abstract：:For the class A β-lactamase SHV-1, the kinetic and mechanistic properties of the clinically used inhibitor sulbactam are compared with the sulbactam analog substituted in its 6β position by a CH2OH group (6β-(hydroxymethyl)penicillanic acid). The 6β substitution improves both in vitro and microbiological inhibitory pr...

journal_title：Biochemistry

authors： Che T,Rodkey EA,Bethel CR,Shanmugam S,Ding Z,Pusztai-Carey M,Nottingham M,Chai W,Buynak JD,Bonomo RA,van den Akker F,Carey PR

更新日期：2015-01-27 00:00:00

abstract：:Cellular toxicity resulting from nucleation-dependent polymerization of amyloid beta-peptide (Abeta) is considered to be a major and possibly the primary component of Alzheimer's disease (AD). Inhibition of Abeta polymerization has thus been identified as a target for the development of therapeutic agents for the trea...

journal_title：Biochemistry

authors： Findeis MA,Musso GM,Arico-Muendel CC,Benjamin HW,Hundal AM,Lee JJ,Chin J,Kelley M,Wakefield J,Hayward NJ,Molineaux SM

更新日期：1999-05-25 00:00:00