Biosynthesis of glycosphingolipids by human myeloid leukemia cells.

abstract：:C-type natriuretic peptide (CNP) binds the guanylyl cyclase-linked natriuretic peptide receptor B (NPR-B) and stimulates marked elevations of the intracellular signaling molecule, cGMP. Here, the essential role of phosphorylation in the hormonal activation and deactivation of this receptor is described. Exposure of NI...

journal_title：Biochemistry

authors： Potter LR

更新日期：1998-02-24 00:00:00

abstract：:NanGIR1 is a catalytic element inserted in the P6 loop of a group I intron (NanGIR2) in the small subunit rRNA precursor of the protist Naegleria andersoni [Einvik, C., Decatur, W. A., Embley, T. M., Vogt, V. M., and Johansen, S. (1997) RNA 3, 710-720]. It catalyzes site-specific hydrolysis at an internal processing s...

journal_title：Biochemistry

authors： Jabri E,Aigner S,Cech TR

更新日期：1997-12-23 00:00:00

abstract：:The human pathogen Staphylococcus aureus acquires heme iron from hemoglobin (Hb) via the action of a series of iron-regulated surface determinant (Isd) proteins. The cell wall anchored IsdB protein is recognized as the predominant Hb receptor, and is comprised of two NEAr transporter (NEAT) domains that act in concert...

journal_title：Biochemistry

authors： Fonner BA,Tripet BP,Eilers BJ,Stanisich J,Sullivan-Springhetti RK,Moore R,Liu M,Lei B,Copié V

更新日期：2014-06-24 00:00:00

abstract：:Snake venom contains a number of the hemostatically active C-type lectin-like proteins, which affect the interaction between von Willebrand factor (vWF) and the platelet glycoprotein (GP) Ib or platelet receptor to inhibit/induce platelet activation. Flavocetin-A (FL-A) is a high-molecular mass C-type lectin-like prot...

journal_title：Biochemistry

authors： Fukuda K,Mizuno H,Atoda H,Morita T

更新日期：2000-02-29 00:00:00

abstract：:As for many enzymes, the enzymatic pathway of triosephosphate isomerase (TIM) includes the partially rate determining motion of an active site loop (loop 6, residues 166-176), which must remain closed during chemistry but must open just before product release. The motion of this loop was monitored using laser induced ...

journal_title：Biochemistry

authors： Desamero R,Rozovsky S,Zhadin N,McDermott A,Callender R

更新日期：2003-03-18 00:00:00

abstract：:Isoaspartyl dipeptidase (IAD) is a member of the amidohydrolase superfamily and catalyzes the hydrolytic cleavage of beta-aspartyl dipeptides. Structural studies of the wild-type enzyme have demonstrated that the active site consists of a binuclear metal center positioned at the C-terminal end of a (beta/alpha)(8)-bar...

journal_title：Biochemistry

authors： Martí-Arbona R,Fresquet V,Thoden JB,Davis ML,Holden HM,Raushel FM

更新日期：2005-05-17 00:00:00

abstract：:Tat, an essential human immunodeficiency virus type 1 protein interacts with the transactivation response element (TAR) and stimulates transcription from the viral long-terminal repeat (LTR). Blockage of Tat-TAR interaction halts viral transcription and hence replication. We have found that polyamide nucleic acid (PNA...

journal_title：Biochemistry

authors： Mayhood T,Kaushik N,Pandey PK,Kashanchi F,Deng L,Pandey VN

更新日期：2000-09-26 00:00:00

abstract：:The mutations spectra of cis-syn, trans-syn-I, (6-4), and Dewar pyrimidone photoproducts of the TT site of AATTAA and TATTAT in the (-) strand of a heteroduplex M13 vector were obtained in an excision and photoreversal repair deficient Escherichia coli host under SOS conditions. Oligonucleotides containing site-specif...

journal_title：Biochemistry

authors： Smith CA,Wang M,Jiang N,Che L,Zhao X,Taylor JS

更新日期：1996-04-02 00:00:00

abstract：:One-dimensional 1H NMR was employed to study the effects of Ca2+ and Lu3+ binding on the apo and calcium-saturated forms of dog bone Gla protein (BGP, osteocalcin). Titration of apo dog BGP with Ca2+ in 20 mM NaCl showed spectral perturbations consistent with the binding of 5 mol equiv of calcium in the NMR slow-excha...

journal_title：Biochemistry

authors： Isbell DT,Du S,Schroering AG,Colombo G,Shelling JG

更新日期：1993-10-26 00:00:00

abstract：:Lateral mobilities of fluorescent cell surface probes have been measured on normal (3T3) and transformed (SV3T3) cultured mouse fibroblasts. There is little discernible difference in the mobilities of a lipid analogue (diI), a fluorescent ganglioside derivative (GM1), and tetramethylrhodamine-labeled succinylated conc...

journal_title：Biochemistry

authors： Eldridge CA,Elson EL,Webb WW

更新日期：1980-05-13 00:00:00

abstract：:The cystic fibrosis transmembrane conductance regulator forms an anion-selective channel. We previously showed that charge selectivity, the ability to discriminate between anions and cations, occurs near the cytoplasmic end of the channel. The molecular determinants of charge selectivity, however, are unknown. We inve...

journal_title：Biochemistry

authors： Guinamard R,Akabas MH

更新日期：1999-04-27 00:00:00

abstract：:Cibacron Blue F3GA dye has been used to probe subtle conformational changes in protein structure associated with the conversion of Escherichia coli glutamine synthetase (GS) between relaxed, taut, oxidized, and dissociated forms. Binding of the dye to each form of the enzyme elicits a different spectral perturbation o...

journal_title：Biochemistry

authors： Federici MM,Chock PB,Stadtman ER

更新日期：1985-01-29 00:00:00

abstract：:Class II tRNA synthetases have long been known to have quaternary structures of alpha, alpha2, alpha2beta2, and alpha4, depending on the amino acid specificity and the organism from which the synthetase was isolated. Even the quaternary structures of enzymes for the same amino acid show variations in evolution. The ba...

journal_title：Biochemistry

authors： Ribas de Pouplana L,Schimmel P

更新日期：1997-12-09 00:00:00

abstract：:A common motif in protein structures is the assembly of alpha-helices. Natural alpha-helical assemblies, such as helical bundles and coiled coils, consist of multiple right-handed alpha-helices. Here we design a protein complex containing both left-handed and right-handed helices, with peptides of D- and L-amino acids...

journal_title：Biochemistry

authors： Sia SK,Kim PS

更新日期：2001-07-31 00:00:00

abstract：:Nitric oxide (NO) synthases (NOS) are thiolate-ligated heme-, tetrahydrobiopterin (BH(4))-, and flavin-containing monooxygenases which catalyze the NADPH-dependent conversion of L-arginine (L-Arg) to NO AND citrulline. NOS consists of two domains: an N-terminal oxygenase (heme- and BH(4)-bound) domain and a C-terminal...

journal_title：Biochemistry

authors： Sono M,Ledbetter AP,McMillan K,Roman LJ,Shea TM,Masters BS,Dawson JH

更新日期：1999-11-30 00:00:00

abstract：:The R2 protein of ribonucleotide reductase catalyzes the dioxygen-dependent one-electron oxidation of Tyr122 at a diiron-carboxylate site. Methane monooxygenase and related hydroxylases catalyze hydrocarbon hydroxylation at diiron sites structurally related to the one in R2. In protein R2, the likely reaction site for...

journal_title：Biochemistry

authors： Logan DT,deMaré F,Persson BO,Slaby A,Sjöberg BM,Nordlund P

更新日期：1998-07-28 00:00:00

abstract：:Combined quantum mechanical/molecular mechanical (QM/MM) simulations are carried out to analyze factors that dictate the proton transfer in carbonic anhydrase II (CAII), an enzyme that has been used as a prototypical example of long-range proton transfers in biomolecules. In contrast to the long-held conjecture in the...

journal_title：Biochemistry

authors： Riccardi D,König P,Guo H,Cui Q

更新日期：2008-02-26 00:00:00

abstract：:Comparison between the structures and solvent-accessible surfaces of the antigen-binding fragments of two murine anti-p-azophenylarsonate monoclonal antibodies, one bearing a major cross-reactive idiotype of A/J strain mice (36-71) and one lacking the idiotype (R19.9; Lascombe et al., 1989), highlight the structural b...

journal_title：Biochemistry

authors： Strong RK,Petsko GA,Sharon J,Margolies MN

更新日期：1991-04-16 00:00:00

abstract：:Membrane preparations from two independent ouabain-resistant HeLa cell clones, HI-B1 and HI-C1, each appear to contain two species of (Na,K)ATPase. Two-thirds of the total (Na,K)ATPase in each mutant is indistinguishable from the enzyme in preparations of wild type cells with respect to ouabain binding, ouabain inhibi...

journal_title：Biochemistry

authors： Robbins AR,Baker RM

更新日期：1977-11-15 00:00:00

abstract：:Intracellular retinoid-binding proteins are small, tightly folded, compact proteins, which appear to be involved in the delivery of retinoids to microsomal metabolic enzymes, among other potential roles. Recently, it has been demonstrated that two of these binding proteins, cellular retinol-binding protein (CRBP) and ...

journal_title：Biochemistry

authors： Jamison RS,Newcomer ME,Ong DE

更新日期：1994-03-15 00:00:00

abstract：:The thermotropic phase behavior of 10 mixed-chain phosphatidylcholines, in excess water, has been examined and compared with that of identical-chain C(16):C(16)PC by using high-resolution differential scanning calorimetry (DSC). The molecular weights (MW) of these 11 molecular species are the same, but their delta C/C...

journal_title：Biochemistry

authors： Bultmann T,Lin HN,Wang ZQ,Huang CH

更新日期：1991-07-23 00:00:00

abstract：:Previous studies have shown that murine macrophages immunostimulated with interferon gamma and Escherichia coli lipopolysaccharide synthesize NO2-, NO3-, and citrulline from L-arginine by oxidation of one of the two chemically equivalent guanido nitrogens. The enzymatic activity for this very unusual reaction was foun...

journal_title：Biochemistry

authors： Marletta MA,Yoon PS,Iyengar R,Leaf CD,Wishnok JS

更新日期：1988-11-29 00:00:00

abstract：:Organisms and cellular systems which have adapted to stresses such as high temperature, desiccation, and urea-concentrating environments have responded by concentrating particular organic solutes known as osmolytes. These osmolytes are believed to confer protection to enzyme and other macromolecular systems against su...

journal_title：Biochemistry

authors： Santoro MM,Liu Y,Khan SM,Hou LX,Bolen DW

更新日期：1992-06-16 00:00:00

abstract：:Human and mouse metallothionein-3 (MT-3) molecules exhibit the same metal binding stoichiometry with Zn(II), Cd(II), or Cu(I) as MT-1 or MT-2 molecules, suggesting that MT-3 consists of two domains enfolding separate polymetallic clusters. The kinetic reactivities of Zn(II) complexes of MT-3 with the chelator ethylene...

journal_title：Biochemistry

authors： Sewell AK,Jensen LT,Erickson JC,Palmiter RD,Winge DR

更新日期：1995-04-11 00:00:00

abstract：:The direct electrochemistry of the cytochrome c/cytochrome b5 and cytochrome c/plastocyanin complexes has been investigated at edge-plane graphite and modified gold electrode surfaces, which are selective for one of the two components of the complex. Electrochemical response of one protein at an otherwise electrostati...

journal_title：Biochemistry

authors： Bagby S,Barker PD,Guo LH,Hill HA

更新日期：1990-04-03 00:00:00

abstract：:Using our hemoglobin expression system in Escherichia coli, we have constructed three recombinant hemoglobins (rHbs) with amino acid substitutions located in the alpha(1)beta(1) and alpha(1)beta(2) subunit interfaces and in the distal heme pocket of the alpha-chain: rHb(alphaV96W, betaN108K), rHb(alphaL29F, alphaV96W,...

journal_title：Biochemistry

authors： Jeong ST,Ho NT,Hendrich MP,Ho C

更新日期：1999-10-05 00:00:00

abstract：:In bee venom phospholipase A2, histidine-34 probably functions as a Brønsted base to deprotonate the attacking water. Aspartate-64 and tyrosine-87 form a hydrogen bonding network with histidine-34. We have prepared mutants at these positions and studied their kinetic properties. The mutant in which histidine-34 is cha...

journal_title：Biochemistry

authors： Annand RR,Kontoyianni M,Penzotti JE,Dudler T,Lybrand TP,Gelb MH

更新日期：1996-04-09 00:00:00

abstract：:Low-temperature Fourier transform infrared spectroscopy has been used to study mutants of Glu194 and Glu204, two amino acids that are involved in proton release to the extracellular side of bacteriorhodopsin. Difference spectra of films of E194Q, E204Q, E194Q/E204Q, E9Q/E194Q/E204Q, and E9Q/E74Q/E194Q/E204Q at 243, 27...

journal_title：Biochemistry

authors： Lazarova T,Sanz C,Sepulcre F,Querol E,Padrós E

更新日期：2002-06-25 00:00:00

abstract：:Thermodynamic equilibrium transition models in DSC are only applicable to reversible processes, but reversibility of the thermal transitions of proteins is comparatively rare because of intermolecular aggregation of denatured proteins and the degradation that occurs at high temperatures. The cupredoxin azurin from Pse...

journal_title：Biochemistry

authors： Sandberg A,Leckner J,Shi Y,Schwarz FP,Karlsson BG

更新日期：2002-01-22 00:00:00

abstract：:5,6-Dihydrothymidine 5'-triphosphate (DHdTTP) was synthesized by catalytic hydrogenation of thymidine 5'-triphosphate (dTTP). Thymidine glycol 5'-triphosphate (dTTP-GLY) was prepared by bromination of dTTP followed by treatment with Ag2O. The modified nucleotides were extensively purified by anion-exchange high-perfor...

journal_title：Biochemistry

authors： Ide H,Melamede RJ,Wallace SS

更新日期：1987-02-10 00:00:00