Crystal structure of flavocetin-A, a platelet glycoprotein Ib-binding protein, reveals a novel cyclic tetramer of C-type lectin-like heterodimers.

abstract：:For the study of the protein--protein and protein--nucleic acid interactions in the assembly of virus particles, laser Raman spectra have been obtained in H2O and D2O solutions and as a function of temperature for the following Salmonella phage P22 components: mature phage particles, isolated mature phage DNA, mature ...

journal_title：Biochemistry

authors： Thomas GJ Jr,Li Y,Fuller MT,King J

更新日期：1982-08-03 00:00:00

abstract：:Proelastase has been purified to homogeneity from rat pancreatic tissue by a combination of CM-Sephadex and immobilized protease inhibitor affinity resins. Trypsin activation yields an elastolytic enzyme that possesses a specificity toward small hydrophobic residues in synthetic amide substrates, similar to those of p...

journal_title：Biochemistry

authors： Largman C

更新日期：1983-08-02 00:00:00

abstract：:Evidence of nonequilibrium local heating in transient spectra of LHCII, the main light-harvesting complex of plants, was studied by using various excitation intensities over a wide temperature range, from 10 K to room temperature. No obvious manifestation of local heating was found at room temperature, whereas at 10 K...

journal_title：Biochemistry

authors： Gulbinas V,Karpicz R,Garab G,Valkunas L

更新日期：2006-08-08 00:00:00

abstract：:G proteins play an important role in transmitting hormonal signals, and fluorescence techniques would be useful to study their cellular distribution and mechanisms. To prepare active fluorescent G protein Go/Gi or beta gamma subunits were reacted with fluorescein isothiocyanate (FITC) to label the alpha (F-alpha) and ...

journal_title：Biochemistry

authors： Kwon G,Remmers AE,Datta S,Neubig RR

更新日期：1993-03-09 00:00:00

abstract：:A steady-state kinetic analysis was conducted of the overall aminoacylation reaction catalyzed by isoleucyl-tRNA synthetase. The patterns of Lineweaver-Burk plots obtained indicated that tRNA adds to the enzyme only after isoleucyl adenylate formation and pyrophosphate release. These kinetic patterns were consistent w...

journal_title：Biochemistry

authors： Moe JG,Piszkiewicz D

更新日期：1979-06-26 00:00:00

abstract：:Cryptophycin-52 (LY355703) is currently undergoing clinical evaluation for cancer chemotherapy. It is a potent suppressor of microtubule dynamics in vitro, and low picomolar concentrations appear to inhibit cancer cell proliferation at mitosis by stabilizing spindle microtubules. In the present study, using [(3)H]cryp...

journal_title：Biochemistry

authors： Panda D,Ananthnarayan V,Larson G,Shih C,Jordan MA,Wilson L

更新日期：2000-11-21 00:00:00

abstract：:The gene 5 protein (g5p) encoded by filamentous Ff phages is an ssDNA-binding protein, which binds to and sequesters the nascent ssDNA phage genome in the process of phage morphogenesis. The g5p also binds with high affinity to DNA and RNA sequences that form G-quadruplex structures. However, sequences that would form...

journal_title：Biochemistry

authors： Wen JD,Gray DM

更新日期：2004-03-09 00:00:00

abstract：:A double mutant cycle has been used to evaluate interaction energies between the global stabilizer mutation asparagine 52 --> isoleucine (N52I) in iso-1-cytochrome c and mutations producing single surface histidines at positions 26, 33, 39, 54, 73, 89, and 100. These histidine mutation sites are distributed through th...

journal_title：Biochemistry

authors： Wandschneider E,Hammack BN,Bowler BE

更新日期：2003-09-16 00:00:00

abstract：:A variant of the Tetrahymena ribozyme that efficiently cleaves single-stranded DNA under simulated physiological conditions [Tsang, J., & Joyce, G. F. (1994) Biochemistry 33, 5966-5973] was evaluated as a potential therapeutic agent on the basis of its ability to cleave synthetic oligonucleotide substrates correspondi...

journal_title：Biochemistry

authors： Raillard SA,Joyce GF

更新日期：1996-09-10 00:00:00

abstract：:The prokaryotic DNA protection during starvation (Dps) proteins typically protect macromolecules against damaging agents via physical association with DNA and by oxidizing and sequestering iron. However, Deinococcus radiodurans Dps-1, which binds DNA with high affinity, fails to protect DNA against hydroxyl radicals d...

journal_title：Biochemistry

authors： Nguyen KH,Grove A

更新日期：2012-08-21 00:00:00

abstract：:The oxidation of deoxy- and oxyhemerythrin to methemerythrin by Fe(CN)6(3-) has been examined. The first step produces (semi-met)O, a half-oxidized form of hemerythrin which has different spectral and kinetic properties from (semi-met)R which is produced by one-electron reduction of methemerythrin. Further oxidation o...

journal_title：Biochemistry

authors： Bradić Z,Harrington PC,Wilkins RG,Yoneda G

更新日期：1980-09-02 00:00:00

abstract：:We have investigated, both experimentally and theoretically, the efflux of carboxyfluorescein (a self-quenching fluorescent dye) from vesicles of different sizes and lipid species (POPC, DOPC) after having added the bee venom peptide melittin. This comprises quantitative analyses regarding the extent of lipid-associat...

journal_title：Biochemistry

authors： Rex S,Schwarz G

更新日期：1998-02-24 00:00:00

abstract：:RXPA380 (Cbz-PhePsi[PO(2)CH]Pro-Trp-OH) was reported recently as the first highly selective inhibitor of the C-domain of somatic angiotensin-converting enzyme (ACE), able to differentiate the two active sites of somatic ACE by a selectivity factor of more than 3 orders of magnitude. The contribution of each RXPA380 re...

journal_title：Biochemistry

authors： Georgiadis D,Cuniasse P,Cotton J,Yiotakis A,Dive V

更新日期：2004-06-29 00:00:00

abstract：:By exposing photosystem II (PSII) samples to an incrementing number of excitation flashes at room temperature, followed by freezing, we could compare the Mn-derived multiline EPR signal from the S2 oxidation state as prepared by 1, 5, 10, and 25 flashes of light. While the S2 multiline signals exhibited by these sampl...

journal_title：Biochemistry

authors： Peterson S,Ahrling KA,Högblom JE,Styring S

更新日期：2003-03-11 00:00:00

abstract：:We have designed a novel estrogen-responsive unit, overERE, which consists of two overlapping ERE separated by 5 bp (center-to-center). In gel retardation assays, this sequence forms a low-mobility complex that migrates like an estrogen receptor tetramer. The receptor-overERE complex was specific and was supershifted ...

journal_title：Biochemistry

authors： Massaad C,Coumoul X,Sabbah M,Garlatti M,Redeuilh G,Barouki R

更新日期：1998-04-28 00:00:00

abstract：:The crystal structure of human platelet factor 4 (PF4) has been solved to a resolution of 2.4 A by molecular replacement and refined to an R-factor of 24.1%. The structure consists of four polypeptide chains which form a tetrameric unit. N-terminal residues, previously defined as a random coil or extended loop region,...

journal_title：Biochemistry

authors： Zhang X,Chen L,Bancroft DP,Lai CK,Maione TE

更新日期：1994-07-12 00:00:00

abstract：:We introduced a stop codon in place of the ATT codon encoding Ile283 (numbered from the Met initiation codon) in the petA gene from Chlamydomonas reinhardtii. The resulting protein was expected to be truncated on its carboxy-terminus end, lacking the last 35 amino acids. This region of the polypeptide sequence encompa...

journal_title：Biochemistry

authors： Kuras R,Wollman FA,Joliot P

更新日期：1995-06-06 00:00:00

abstract：:The effect on overall alpha-helix content of substituting proline for alanine has been determined at 5 positions (1, 2, 4, 5, and 13) of a 13-residue peptide related in sequence to residues 1-13 of ribonuclease A. The helix content falls off rapidly as proline is moved inward, and the proline residue effectively trunc...

journal_title：Biochemistry

authors： Strehlow KG,Robertson AD,Baldwin RL

更新日期：1991-06-11 00:00:00

abstract：:Pyridoxal 5'-phosphate at concentrations greater than 0.5 mM inhibits polymerization of deoxynucleoside triphosphate catalyzed by a variety of DNA polymerases. The requirement for a phosphate as well as aldehyde moiety of pyridoxal phosphate for inhibition to occur is clearly shown by the fact that neither pyridoxal n...

journal_title：Biochemistry

authors： Modak MJ

更新日期：1976-08-10 00:00:00

abstract：:Polyhistidine affinity tags are routinely employed as a convenient means of purifying recombinantly expressed proteins. A tacit assumption is commonly made that His tags have little influence on protein structure and function. Attachment of a His tag to the N-terminus of the robust globular protein myoglobin leads to ...

journal_title：Biochemistry

authors： Thielges MC,Chung JK,Axup JY,Fayer MD

更新日期：2011-06-28 00:00:00

abstract：:The sucrose (CscB) permease belongs to the oligosaccharide:H(+) symporter family of the Major Facilitator Superfamily and is homologous to the lactose permease from Escherichia coli. Sucrose transport in cells expressing sucrose permease is completely inhibited by N-ethylmaleimide (NEM), suggesting that one or more of...

journal_title：Biochemistry

authors： Sahin-Tóth M,Frillingos S,Lawrence MC,Kaback HR

更新日期：2000-05-23 00:00:00

abstract：:Taq and T7 DNA polymerases have become basic molecular biology "tools" for DNA sequence analysis. However, Taq, unlike T7 DNA polymerase, is strongly biased against the incorporation of 2',3'-dideoxynucleotide triphosphates (ddNTPs) indicating very different substrate selectivities. Equilibrium binding and rate consta...

journal_title：Biochemistry

authors： Brandis JW,Edwards SG,Johnson KA

更新日期：1996-02-20 00:00:00

abstract：:The temperature dependence of steady-state kinetics has been studied with horse liver alcohol dehydrogenase (HLADH) using protonated and deuterated benzyl alcohol as substrates in methanol/water mixtures between +3 and -50 degrees C. Additionally, the competitive isotope effects, k(H)/k(T) and k(D)/k(T), were measured...

journal_title：Biochemistry

authors： Tsai S,Klinman JP

更新日期：2001-02-20 00:00:00

abstract：:Strong inward-rectifier K(+) (Kir) channels play a significant role in shaping the cardiac action potential: they help produce its long plateau and accelerate its rate of repolarization. Consequently, genetic deletion of the gene encoding the strongly rectifying K(+) channel IRK1 (Kir2.1) prolongs the cardiac action p...

journal_title：Biochemistry

authors： Xu Y,Lu Z

更新日期：2004-12-14 00:00:00

abstract：:The incorporation of 7-dehydrocholesterol into synthetic phospholipid bilayers altered the distribution of products after photolysis. In liposomes, the relative amounts of 7-dehydrocholesterol and lumisterol were elevated, and tachysterol was reduced from the levels observed in hexane solution. Z to E isomerization of...

journal_title：Biochemistry

authors： Yamamoto JK,Borch RF

更新日期：1985-06-18 00:00:00

abstract：:Plasma membrane and vesicular transporters for the biogenic amines, dopamine, norepinephrine, and serotonin, represent a group of proteins that play a crucial role in the regulation of neurotransmission. Clinically, mono amine transporters are the primary targets for the actions of many therapeutic agents used to trea...

journal_title：Biochemistry

authors： Sager JJ,Torres GE

更新日期：2011-08-30 00:00:00

abstract：:Human apolipoprotein stimulation of sphingomyelin (SM) hydrolysis by sphingomyelinase from human skin fibroblasts has been studied. Apolipoproteins A-I, A-II, B, C-I, and E do not enhance sphingomyelin hydrolysis above control levels. In contrast, apoC-II stimulates sphingomyelin hydrolysis by approximately 2.5-fold. ...

journal_title：Biochemistry

authors： Ahmad TY,Beaudet AL,Sparrow JT,Morrisett JD

更新日期：1986-07-29 00:00:00

abstract：:Stabilization of the plasma membrane Ca-ATPase (PMCA) in an inactive conformation upon oxidation of multiple methionines in the calcium regulatory protein calmodulin (CaM) is part of an adaptive cellular response to minimize ATP utilization and the generation of reactive oxygen species (ROS) under conditions of oxidat...

journal_title：Biochemistry

authors： Chen B,Mayer MU,Squier TC

更新日期：2005-03-29 00:00:00

abstract：:Crystallographic studies of ligand-protein complexes reveal most preferable ligand binding modes, but do not show less populated modes that may contribute to measurable biochemical and biophysical characteristics of the complexes. In some cases, a ligand may bind a protein in essentially different modes. An example is...

journal_title：Biochemistry

authors： Blanchet J,Lin SX,Zhorov BS

更新日期：2005-05-17 00:00:00

abstract：:The dynamics of individual DNA molecules undergoing orthogonal field alternating gel electrophoresis (OFAGE) have been studied by use of T2 DNA molecules labeled with a dye and visualized with a fluorescence microscope. The mechanism of reorientation used by a molecule to align itself in the direction of the new ortho...

journal_title：Biochemistry

authors： Gurrieri S,Rizzarelli E,Beach D,Bustamante C

更新日期：1990-04-03 00:00:00