Synthesis and characterization of fluorescently labeled bovine brain G protein subunits.

abstract：:A plasminogen activator secreted by cultured human pancreatic carcinoma (Mia PaCa-2) cells has been purified to apparent homogeneity by procedures including Sepharose-L-arginine methyl ester affinity chromatography, Sephadex G-200 gel filtration, isoelectric focusing, and sodium dodecyl sulfate gel electrophoresis. Th...

journal_title：Biochemistry

authors： Wu M,Arimura GK,Yunis AA

更新日期：1977-05-03 00:00:00

abstract：:Gluconacetobacter diazotrophicus stands out among the acetic acid bacteria as it fixes dinitrogen and is a true endophyte. It has a set of constitutive enzymes to oxidize ethanol and acetaldehyde which is upregulated during N(2)-dependent growth. The membrane-bound alcohol dehydrogenase (ADH) is a heterodimer (subunit...

journal_title：Biochemistry

authors： Gómez-Manzo S,Solano-Peralta A,Saucedo-Vázquez JP,Escamilla-Marván JE,Kroneck PM,Sosa-Torres ME

更新日期：2010-03-23 00:00:00

abstract：:Antifreeze proteins lower the freezing point of their solution by binding to ice and inhibiting its growth. One of several structurally different antifreeze proteins in fishes (type II) is homologous to the carbohydrate-recognition domain of Ca2+-dependent lectins and adopts the same three-dimensional fold. Type II an...

journal_title：Biochemistry

authors： Loewen MC,Gronwald W,Sönnichsen FD,Sykes BD,Davies PL

更新日期：1998-12-22 00:00:00

abstract：:Aromatic residues in c-type cytochromes might have an important function in the folding and/or electron transferring properties of the molecule. In the tetraheme cytochrome c3 (Mr 13 000) from Desulfovibrio vulgaris Hildenborough, Phe20, is located between heme 1 and heme 3 with its aromatic ring close and almost para...

journal_title：Biochemistry

authors： Dolla A,Arnoux P,Protasevich I,Lobachov V,Brugna M,Giudici-Orticoni MT,Haser R,Czjzek M,Makarov A,Bruschi M

更新日期：1999-01-05 00:00:00

abstract：:The dehaloperoxidase-hemoglobin (DHP) from the terebellid polychaete Amphitrite ornata is a multifunctional hemoprotein that catalyzes the oxidation of a wide variety of substrates, including halo/nitrophenols, haloindoles, and pyrroles, via peroxidase and/or peroxygenase mechanisms. To probe whether substrate substit...

journal_title：Biochemistry

authors： McGuire AH,Carey LM,de Serrano V,Dali S,Ghiladi RA

更新日期：2018-07-31 00:00:00

abstract：:Photoactivated adenylyl cyclases are powerful tools for optogenetics and for investigating signal transduction mechanisms in biological photoreceptors. Because of its large increase in enzyme activity in the light, the BLUF (blue light sensor using flavin adenine dinucleotide)-activated adenylyl cyclase (bPAC) from Be...

journal_title：Biochemistry

authors： Stierl M,Penzkofer A,Kennis JT,Hegemann P,Mathes T

更新日期：2014-08-12 00:00:00

abstract：:Site-directed mutagenesis studies have confirmed that the distal histidine in myoglobin stabilizes bound O2 by hydrogen bonding and have suggested that it is the polar character of the imidazole side chain rather than its size that limits the rate of ligand entry into the protein. We constructed an isosteric Val68 to ...

journal_title：Biochemistry

authors： Smerdon SJ,Dodson GG,Wilkinson AJ,Gibson QH,Blackmore RS

更新日期：1991-06-25 00:00:00

abstract：:In order to elucidate the molecular mechanisms responsible for the apparent nonlinear behavior of the temperature dependence of the redox potential of Hydrogenobacter thermophilus cytochrome c552 [Takahashi, Y., Sasaki, H., Takayama, S. J., Mikami, S., Kawano, S., Mita, H., Sambongi, Y., and Yamamoto, Y. (2006) Bioche...

journal_title：Biochemistry

authors： Takayama SJ,Takahashi YT,Mikami S,Irie K,Kawano S,Yamamoto Y,Hemmi H,Kitahara R,Yokoyama S,Akasaka K

更新日期：2007-08-14 00:00:00

abstract：:Conformational selection and induced fit are two well-known mechanisms of allosteric protein-ligand interaction. Some proteins, like ubiquitin, have recently been found to exist in multiple conformations at equilibrium, suggesting that the conformational selection may be a general mechanism of interaction, in particul...

journal_title：Biochemistry

authors： Chi CN,Bach A,Engström A,Wang H,Strømgaard K,Gianni S,Jemth P

更新日期：2009-08-04 00:00:00

abstract：:Elongation factor-2 kinase (eEF-2K) is a Ca(2+)/calmodulin-dependent protein kinase that phosphorylates and inactivates eEF-2 and that can regulate the rate of protein synthesis at the elongation stage. Here we report that a slight decrease in pH, within the range observed in vivo, leads to a dramatic activation of eE...

journal_title：Biochemistry

authors： Dorovkov MV,Pavur KS,Petrov AN,Ryazanov AG

更新日期：2002-11-12 00:00:00

abstract：:Mg2+ is important for the RNase activity of the hammerhead ribozyme. To investigate the binding properties of Mg2+ to the hammerhead ribozyme, cleavage rates and CD spectra for substrates containing inosine or guanosine at the cleavage site were measured. The 2-amino group of this guanosine interfered with the rate of...

journal_title：Biochemistry

authors： Koizumi M,Ohtsuka E

更新日期：1991-05-28 00:00:00

abstract：:The annexin fold consists of four 70-residue domains with markedly homologous sequences and nearly identical structures. Each domain contains five helices designated A to E. Domain 2 of annexin I was obtained by chemical synthesis including ten specifically labeled residues and studied by 1H-15N NMR and circular dichr...

journal_title：Biochemistry

authors： Cordier-Ochsenbein F,Guerois R,Baleux F,Huynh-Dinh T,Chaffotte A,Neumann JM,Sanson A

更新日期：1996-08-13 00:00:00

abstract：:A nucleoside triphosphate binding site on calf thymus RNA polymerase II was identified by using photoaffinity analogues of adenosine 5'-triphosphate and guanosine 5'-triphosphate. Both radiolabeled 8-azidoadenosine 5'-triphosphate (8-N3ATP) and radiolabeled 8-azidoguanosine 5'-triphosphate (8-N3GTP) bound to a single ...

journal_title：Biochemistry

authors： Freund E,McGuire PM

更新日期：1986-01-14 00:00:00

abstract：:Lon is a homo-oligomeric ATP-dependent serine protease that functions in the degradation of damaged and certain regulatory proteins. This enzyme has emerged as a novel target in the development of antibiotics because of its importance in conferring bacterial virulence. In this study, we explored the mechanism by which...

journal_title：Biochemistry

authors： Frase H,Lee I

更新日期：2007-06-05 00:00:00

abstract：:De novo purine biosynthesis proceeds by two divergent paths. In bacteria, yeasts, and plants, 5-aminoimidazole ribonucleotide (AIR) is converted to 4-carboxy-AIR (CAIR) by two enzymes: N(5)-carboxy-AIR (N(5)-CAIR) synthetase (PurK) and N(5)-CAIR mutase (class I PurE). In animals, the conversion of AIR to CAIR requires...

journal_title：Biochemistry

authors： Tranchimand S,Starks CM,Mathews II,Hockings SC,Kappock TJ

更新日期：2011-05-31 00:00:00

abstract：:Thiol dioxygenases are mononuclear non-heme FeII-dependent metalloenzymes that initiate the oxidative catabolism of thiol-containing substrates to their respective sulfinates. Cysteine dioxygenase (CDO), the best characterized mammalian thiol dioxygenase, contains a three-histidine (3-His) coordination environment rat...

journal_title：Biochemistry

authors： Fernandez RL,Dillon SL,Stipanuk MH,Fox BG,Brunold TC

更新日期：2020-07-07 00:00:00

abstract：:The transporter associated with antigen processing (TAP) is a prototype of an asymmetric ATP-binding cassette (ABC) transporter, which uses ATP binding and hydrolysis to translocate peptides from the cytosol to the lumen of the endoplasmic reticulum (ER). Here, we review molecular details of peptide binding and ATP bi...

journal_title：Biochemistry

authors： Hinz A,Tampé R

更新日期：2012-06-26 00:00:00

abstract：:Chlorophyll a fluorescence emission is widely used as a noninvasive measure of a number of parameters related to photosynthetic efficiency in oxygenic photosynthetic organisms. The most important component for the estimation of photochemistry is the relative increase in fluorescence yield between dark-adapted samples ...

journal_title：Biochemistry

authors： Vasil'ev S,Bruce D

更新日期：1998-08-04 00:00:00

abstract：:The role of the peripheral reaction center chlorophyll a molecule associated with His117 of the D2 polypeptide in photosystem II was investigated in Synechocystis sp. PCC 6803 using a combination of steady state, pump-probe, and picosecond time-resolved fluorescence spectroscopy. Data were obtained from intact cells a...

journal_title：Biochemistry

authors： Vasil'ev S,Bruce D

更新日期：2000-11-21 00:00:00

abstract：:PI-SceI is a member of an unusual class of rare cutting homing endonucleases produced by an autocatalytic protein splicing from a precursor. To analyze the structural and functional domain organization of the endonuclease PI-SceI and to examine whether the DNA binding activity can be structurally separated from the ca...

journal_title：Biochemistry

authors： Pingoud V,Grindl W,Wende W,Thole H,Pingoud A

更新日期：1998-06-02 00:00:00

abstract：:Competitive inhibition of the monophenolase reaction of tyrosinase by a variety of compounds was investigated with respect to temperature and pH. Derivatives of benzoic acid as well as p-nitrophenol were the best inhibitors. Toluene and several N-heterocycles were all weak inhibitors. Thermodynamic parameters of tolue...

journal_title：Biochemistry

authors： Conrad JS,Dawso SR,Hubbard ER,Meyers TE,Strothkamp KG

更新日期：1994-05-17 00:00:00

abstract：:Our earlier NMR study showed that a two-disulfide variant of hen lysozyme containing intra-alpha-domain disulfide bridges, C6-C127 and C30-C115, is partially folded, with the alpha domain tightly folded to the nativelike conformation and the beta domain flexible or unfolded. With a view that the formation of a third d...

journal_title：Biochemistry

authors： Yokota A,Hirai K,Miyauchi H,Iimura S,Noda Y,Inoue K,Akasaka K,Tachibana H,Segawa S

更新日期：2004-06-01 00:00:00

abstract：:Heparin accelerates inhibition of factor XIa (fXIa) by the serpins antithrombin (AT) and C1-inhibitor (C1-INH) by more than 2 orders of magnitude. The mechanism of the heparin-mediated acceleration of fXIa inhibition by these serpins is incompletely understood, as heparin appears to interact with both the catalytic an...

journal_title：Biochemistry

authors： Yang L,Sun MF,Gailani D,Rezaie AR

更新日期：2009-02-24 00:00:00

abstract：:The p90 ribosomal S6 family of kinases (RSK) are potential drug targets, due to their involvement in cancer and other pathologies. There are currently only two known selective inhibitors of RSK, but the basis for selectivity is not known. One of these inhibitors is a naturally occurring kaempferol-α-L-diacetylrhamnosi...

journal_title：Biochemistry

authors： Utepbergenov D,Derewenda U,Olekhnovich N,Szukalska G,Banerjee B,Hilinski MK,Lannigan DA,Stukenberg PT,Derewenda ZS

更新日期：2012-08-21 00:00:00

abstract：:The noncovalent interaction of 2-aminodipyrido[1,2-a:3',2'-d]imidazole (Glu-P-2) and its derivatives, which are potent mutagens isolated from L-glutamic acid pyrolysate, with calf thymus DNA was studied by steady-state and nanosecond fluorescence spectroscopies. The fluorescence of these compounds exhibits static quen...

journal_title：Biochemistry

authors： Itoh M,Kurokawa H,Usui M,Ohno M,Shimoda E,Hashimoto Y,Shudo K

更新日期：1985-11-05 00:00:00

abstract：:Although plasma membrane domains, such as caveolae, provide an organizing principle for signaling pathways and cholesterol homeostasis in the cell, relatively little is known regarding specific mechanisms, whereby intracellular lipid-binding proteins are targeted to caveolae. Therefore, the interaction between caveoli...

journal_title：Biochemistry

authors： Parr RD,Martin GG,Hostetler HA,Schroeder ME,Mir KD,Kier AB,Ball JM,Schroeder F

更新日期：2007-07-17 00:00:00

abstract：:It has been suggested that the down-regulation of hepatic HMG-CoA reductase by dietary cholesterol requires modification of the cholesterol molecule before it can exert its suppressive action. In a recent study [Lund, E., Breuer, O., & Björkhem, I. (1992) J. Biol. Chem. 267, 25092-25097], we showed that side-chain hyd...

journal_title：Biochemistry

authors： Lund E,Björkhem I

更新日期：1994-01-11 00:00:00

abstract：:The 86-amino acid colicin E9 immunity protein (Im9), which inhibits the DNase activity of colicin E9, has been overexpressed in Escherichia coli and isotopically enriched with 15N and 13C. Using the 3D CBCANH and CBCA(CO)NH experiments, we have almost completely assigned the backbone 13C resonances and extended previo...

journal_title：Biochemistry

authors： Osborne MJ,Breeze AL,Lian LY,Reilly A,James R,Kleanthous C,Moore GR

更新日期：1996-07-23 00:00:00

abstract：:Pulsed electron paramagnetic resonance spectroscopy has been used to detect Mn(II)-ligand superhyperfine couplings in complexes with creatine kinase and in the Mn(II) metalloprotein concanavalin A. Electron spin-echo envelopes from Mn(II), bound in these complexes, are modulated by superhyperfine interactions between ...

journal_title：Biochemistry

authors： LoBrutto R,Smithers GW,Reed GH,Orme-Johnson WH,Tan SL,Leigh JS Jr

更新日期：1986-09-23 00:00:00

abstract：:T4 RNA ligase catalyzes the ATP-dependent addition of a single 2'-deoxyribonucleoside 3',5'-bisphosphate to the 3'-hydroxyl of an oligodeoxyribonucleotide. The bisphosphate is joined to the deoxyoligomer by a 3' leads to 5' phosphodiester bond and the product, which is terminated by a 3'-phosphate, is one nucleotide l...

journal_title：Biochemistry

authors： Hinton DM,Baez JA,Gumport RI

更新日期：1978-11-28 00:00:00