Stimulation of iron(II) bleomycin activity by phosphate-containing compounds.

Abstract:

:Orthophosphate and phosphate derivatives including pyrophosphate, hexametaphosphate, ATP, ADP, and inositol hexaphosphate enhance the extent of DNA degradation by iron(II) bleomycin. These phosphate-containing compounds increase both the release of free nucleic base and that of base propenals which are DNA cleavage products, probably by enhancing the efficiency with which Fe(II) is recruited into the drug. Phosphate action occurs during drug activation prior to the attack on DNA. In addition, phosphates affect the stability of the activated drug complex, overcome the inhibition observed with high concentrations of DNA, and reduce the size of the DNA fragment necessary for reacting with the drug. Phosphate derivatives bind to iron(II) bleomycin and alter its optical spectrum. An analysis of titration data for pyrophosphate and inositol hexaphosphate indicates that each phosphate compound binds to more than one iron(II) bleomycin molecule. With ATP, ADP, and 2,3-diphosphoglycerate, only a single phosphate-containing compound binds to the ferrous drug complex. The affinity for ATP is sufficiently high as to suggest that the ternary complex formed in vitro may exist physiologically.

journal_name

Biochemistry

journal_title

Biochemistry

authors

Burger RM,Horwitz SB,Peisach J

doi

10.1021/bi00335a034

subject

Has Abstract

pub_date

1985-07-02 00:00:00

pages

3623-9

issue

14

eissn

0006-2960

issn

1520-4995

journal_volume

24

pub_type

杂志文章
  • Proton transfer in benzyl alcohol dehydrogenase during catalysis: alternate proton-relay routes.

    abstract::His51 in horse liver alcohol dehydrogenase (ADHE) has been proposed to act as a proton donor/acceptor in the NAD+/NADH-dependent oxidation/reduction of alcohol/aldehyde. The residue corresponding to His51 of ADHE is Val51 (Val45 in the protein sequence) in benzyl alcohol dehydrogenase (BADH) encoded by TOL plasmid pWW...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi970726g

    authors: Inoue J,Tomioka N,Itai A,Harayama S

    更新日期:1998-03-10 00:00:00

  • A major proportion of N-glycoproteins are transiently glucosylated in the endoplasmic reticulum.

    abstract::N-linked, high-mannose-type oligosaccharides lacking glucose residues may be transiently glucosylated directly from UDP-Glc in the endoplasmic reticulum of mammalian, plant, fungal, and protozoan cells. The products formed have been identified as N-linked Glc1Man5-9GlcNAc2 and glucosidase II is apparently the enzyme r...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00226a017

    authors: Gañán S,Cazzulo JJ,Parodi AJ

    更新日期:1991-03-26 00:00:00

  • E6*, the 50 amino acid product of the most abundant spliced transcript of the e6 oncoprotein in high-risk human papillomavirus, is a promiscuous folder and binder.

    abstract::High-risk human papillomavirus E6 participates in tumorigenic progression, mainly by its ability to promote p53 degradation. HPV transcripts show a complex splicing pattern, where E6* is the most abundant transcript in high-risk HPV types, comprising the first 50 amino acids of E6. No structural or biochemical informa...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi101941c

    authors: Heer A,Alonso LG,de Prat-Gay G

    更新日期:2011-03-01 00:00:00

  • Characterization of glycosylation sites of the epidermal growth factor receptor.

    abstract::The epidermal growth factor receptor is a transmembrane glycoprotein that mediates the cellular responses to epidermal growth factor (EGF) and transforming growth factor-alpha (TGF-alpha). In this study of the human EGF receptor naturally expressed in A431 cells, the glycosylation sites of the full-length, membrane-bo...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi027101p

    authors: Zhen Y,Caprioli RM,Staros JV

    更新日期:2003-05-13 00:00:00

  • Communications between the high-affinity cyclic nucleotide binding sites in E. coli cyclic AMP receptor protein: effect of single site mutations.

    abstract::The binding of adenosine 3',5'-cyclic monophosphate (cAMP) and its nonfunctional analogue, guanosine 3',5'-cyclic monophosphate (cGMP), to the adenosine 3',5'-cyclic monophosphate receptor protein (CRP) from Escherichia coli was investigated by means of fluorescence and isothermal titration calorimetry (ITC) at pH 7.8...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi026099z

    authors: Lin SH,Lee JC

    更新日期:2002-10-01 00:00:00

  • Dynamics of light-induced activation in the PAS domain proteins LOV2 and PYP probed by time-resolved tryptophan fluorescence.

    abstract::Light-induced activation of the LOV2-Jα domain of the photoreceptor phototropin from oat is believed to involve the detachment of the Jα helix from the central β-sheet and its subsequent unfolding. The dynamics of these conformational changes were monitored by time-resolved emission spectroscopy with 100 ns time resol...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi101413v

    authors: Hoersch D,Bolourchian F,Otto H,Heyn MP,Bogomolni RA

    更新日期:2010-12-28 00:00:00

  • Origin of the anomalous Fe-CO stretching mode in the CO complex of Ascaris hemoglobin.

    abstract::We report an unusually high frequency (543 cm(-)(1)) for an Fe-CO stretching mode in the CO complex of Ascaris suum hemoglobin as compared to vertebrate hemoglobins in which the frequency of the Fe-CO mode is much lower. A second Fe-CO stretching mode in Ascaris hemoglobin is observed at 515 cm(-1). We propose that th...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi9922087

    authors: Das TK,Friedman JM,Kloek AP,Goldberg DE,Rousseau DL

    更新日期:2000-02-01 00:00:00

  • Activation of the leu-500 promoter: a topological domain generated by divergent transcription in a plasmid.

    abstract::The Salmonella typhimurium leu-500 promoter is active only in topA strains. In an earlier study (Chen, D., Bowater, R., Dorman, C., & Lilley, D. M. J. (1992) Proc. Natl. Acad. Sci. U.S.A. 89, 8784-8788), we showed that the activity of this promoter on a circular plasmid is a function of the transcription and translati...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00211a027

    authors: Chen D,Bowater RP,Lilley DM

    更新日期:1993-12-07 00:00:00

  • Alpha-chain domain of fibrinogen controls generation of fibrinoligase (coagulation factor XIIIa). Calcium ion regulatory aspects.

    abstract::Fibrinogen (approximately 10(-5) M) labilizes heterologous interactions within the thrombin-modified factor XIII zymogen (i.e., XIII' = a2'b2) so that, in the time frame (ca. 10 min) of normal clotting in plasma (37 degrees C, mu = 0.15, pH 7.5), 1.5 mM Ca2+ is sufficient to cause the release of the noncatalytic b sub...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00516a016

    authors: Credo RB,Curtis CG,Lorand L

    更新日期:1981-06-23 00:00:00

  • Human type-alpha transforming growth factor undergoes slow conformational exchange between multiple backbone conformations as characterized by nitrogen-15 relaxation measurements.

    abstract::Human type-alpha transforming growth factor (hTGF alpha) is a small mitogenic protein containing 50 amino acids and three disulfide bonds. It has both sequence and structural homology with epidermal growth factor (EGF). While the three-dimensional structures of hTGF alpha and other EGF-like proteins have been studied ...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00008a003

    authors: Li YC,Montelione GT

    更新日期:1995-02-28 00:00:00

  • Analysis of 31P NMR spectra of enzyme-bound reactants and products of adenylate kinase using density matrix theory of chemical exchange.

    abstract::31P NMR spectra of equilibrium mixtures of enzyme-bound reactants and products of the adenylate kinase reaction (formula; see text) were analyzed by using computer simulations based on density matrix theory of chemical exchange. Since adenylate kinase has the unique feature that the reactants in the reverse direction ...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00300a025

    authors: Vasavada KV,Kaplan JI,Nageswara Rao BD

    更新日期:1984-02-28 00:00:00

  • Independent heterologous fibrillation of insulin and its B-chain peptide.

    abstract::Insulin is very prone to form amyloid fibrils under slightly destabilizing conditions, and the B-chain region plays a critical role in the fibrillation. We show here that the isolated B-chain peptide of bovine insulin also forms fibrils at both acidic and neutral pH. When a mixture of insulin and the B-chain peptide w...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi051658y

    authors: Hong DP,Fink AL

    更新日期:2005-12-20 00:00:00

  • Transient-state kinetics of the ADP-insensitive phosphoenzyme in sarcoplasmic reticulum: implications for transient-state calcium translocation.

    abstract::The kinetics of formation of the ADP-sensitive (EP) and ADP-insensitive (E*P) phosphoenzyme intermediates of the CaATPase in sarcoplasmic reticulum (SR) were investigated by means of the quenched-flow technique. At 21 degrees C, addition of saturating ADP to SR vesicles phosphorylated for 116 ms with 10 microM ATP gav...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00322a018

    authors: Froehlich JP,Heller PF

    更新日期:1985-01-01 00:00:00

  • A Simple Colorimetric System for Detecting Target Antigens by a Three-Stage Signal Transformation-Amplification Strategy.

    abstract::Inexpensive, straightforward, and rapid medical diagnostics are becoming increasingly important for disease identification in time- and resource-limited settings. Previous attempts to link oligonucleotide-based aptamers and hammerhead ribozymes to form ligand-induced ribozymes have been successful in identifying a var...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/acs.biochem.8b00523

    authors: Liao AM,Pan W,Benson JC,Wong AD,Rose BJ,Caltagirone GT

    更新日期:2018-08-28 00:00:00

  • Characterization of the gamma subunits of the 7S nerve growth factor complex.

    abstract::The gamma subunits of the 7S nerve growth factor complex (7S NGF) display arginine esteropeptidase activity. By varying the conditions of electrophoresis in acrylamide gel, it has been demonstrated that the gamma-subunit fraction of 7S NGF contains five different proteins, in contrast to the three (gamma1, gamma2, and...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00652a016

    authors: Stach RW,Server AC,Pignatti PF,Piltch A,Shooter EM

    更新日期:1976-04-06 00:00:00

  • Characterization and topology of the membrane domain Nqo10 subunit of the proton-translocating NADH-quinone oxidoreductase of Paracoccus denitrificans.

    abstract::The proton-translocating NADH-quinone oxidoreductase (NDH-1) of Paracoccus denitrificans is composed of 14 different subunits (Nqo1-Nqo14). Of these, seven subunits (Nqo7, Nqo8, and Nqo10-14) which are equivalent to the mitochondrial DNA-encoded subunits of complex I constitute the membrane segment of the enzyme compl...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi034166z

    authors: Kao MC,Di Bernardo S,Matsuno-Yagi A,Yagi T

    更新日期:2003-04-22 00:00:00

  • Roles of individual domains of annexin I in its vesicle binding and vesicle aggregation: a comprehensive mutagenesis study.

    abstract::To understand the mechanism by which annexin I induces membrane aggregation, a comprehensive mutagenesis of all six Ca2+-binding sites was performed. When the cap residues of type II Ca2+-binding sites were systematically mutated to Ala, a type II site in domain II was shown to be essential for Ca2+-dependent vesicle ...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi980479w

    authors: Bitto E,Cho W

    更新日期:1998-07-14 00:00:00

  • Chemical basis for the selectivity of the von Hippel Lindau tumor suppressor pVHL for prolyl-hydroxylated HIF-1alpha.

    abstract::In animals, the post-translational hydroxylation of hypoxia inducible factor (HIF) is a central mechanism for regulating gene expression in an oxygen-dependent manner. The oxygenase-catalyzed trans-4-prolyl hydroxylation of HIF-alpha increases its affinity for the von Hippel Lindau protein elongin B/C (VCB) ubiquitin ...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi100358t

    authors: Illingworth CJ,Loenarz C,Schofield CJ,Domene C

    更新日期:2010-08-17 00:00:00

  • Intramolecular triple-helix formation at (PunPyn).(PunPyn) tracts: recognition of alternate strands via Pu.PuPy and Py.PuPy base triplets.

    abstract::Triple-helical DNA shows increasing potential for applications in the control of gene expression (including therapeutics) and the development of sequence-specific DNA-cleaving agents. The major limitation in this technology has been the requirement of homopurine sequences for triplex formation. We describe a simple ap...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00117a002

    authors: Jayasena SD,Johnston BH

    更新日期:1992-01-21 00:00:00

  • Evolutionary conservation of enzymatic catalysis: quantitative comparison of the effects of mutation of aligned residues in Saccharomyces cerevisiae and Escherichia coli inorganic pyrophosphatases on enzymatic activity.

    abstract::Soluble inorganic pyrophosphatase (PPase) is one of the better understood phosphoryl-transfer enzymes and is distinctive in having four divalent metal ions at the active site. Here we determine pH profiles for wild-type Saccharomyces cerevisiae PPase (Y-PPase) and for 14 of its active site variants and consider the ef...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi971771r

    authors: Pohjanjoki P,Lahti R,Goldman A,Cooperman BS

    更新日期:1998-02-17 00:00:00

  • Cardiolipin-cholesterol interactions in the liquid-crystalline phase: a steady-state and time-resolved fluorescence anisotropy study with cis- and trans-parinaric acids as probes.

    abstract::The potency of cholesterol to affect the acyl chain order and dynamics of cardiolipin membranes in the liquid-crystalline state was monitored by steady-state and time-resolved fluorescence anisotropy as well as excited-state lifetime measurements with cis- and trans-parinaric acids as probes. Up to a cholesterol mole ...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00357a054

    authors: Gallay J,Vincent M

    更新日期:1986-05-06 00:00:00

  • Perturbation of microtubule polymerization by quercetin through tubulin binding: a novel mechanism of its antiproliferative activity.

    abstract::The dietary flavonoid quercetin has a broad range of biological activities, including potent antitumor activity against several types of tumors. Recently, it has been shown that quercetin inhibits cancer cells proliferation by depleting cellular microtubules and perturbing cellular microtubule functions. However, the ...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi025952r

    authors: Gupta K,Panda D

    更新日期:2002-10-29 00:00:00

  • Orientation and motion of spin-labels in rabbit small intestinal brush border vesicle membranes.

    abstract::The temperature dependence of the packing (order) and fluidity (microviscosity) of rabbit small, intestinal brush border vesicle membranes and of liposomes made from their extracted lipids has been investigated by using a variety of lipid spin probes. The lipids in the brush border membrane are present essentially as ...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00265a036

    authors: Hauser H,Gains N,Semenza G,Spiess M

    更新日期:1982-10-26 00:00:00

  • Communication between the active sites in dimeric mercuric ion reductase: an alternating sites hypothesis for catalysis.

    abstract::Mercuric reductase, a flavoprotein disulfide oxidoreductase, catalyzes the two-electron reduction of Hg(II) to Hg(0) by NADPH. As with all the members of this class of proteins, the enzyme is a dimer of identical subunits with two active sites per dimer, each composed of one FAD and catalytically essential residues fr...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00224a006

    authors: Miller SM,Massey V,Williams CH Jr,Ballou DP,Walsh CT

    更新日期:1991-03-12 00:00:00

  • Control of the redox potential of cytochrome c and microscopic dielectric effects in proteins.

    abstract::X-ray structural information provides the opportunity to explore quantitatively the relation between the microenvironments of heme proteins and their redox potentials. This can be done by considering the protein as a "solvent" for its redox center and calculating the difference between the electrostatic energy of the ...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00355a035

    authors: Churg AK,Warshel A

    更新日期:1986-04-08 00:00:00

  • (Na, K)ATPase activity in membrane preparations of ouabain-resistant HeLa cells.

    abstract::Membrane preparations from two independent ouabain-resistant HeLa cell clones, HI-B1 and HI-C1, each appear to contain two species of (Na,K)ATPase. Two-thirds of the total (Na,K)ATPase in each mutant is indistinguishable from the enzyme in preparations of wild type cells with respect to ouabain binding, ouabain inhibi...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00642a600

    authors: Robbins AR,Baker RM

    更新日期:1977-11-15 00:00:00

  • Akt-dependent antiapoptotic action of insulin is sensitive to farnesyltransferase inhibitor.

    abstract::CHO cells expressing the human insulin receptors (IR) were used to evaluate the effect of the potent farnesyltransferase inhibitor, manumycin, on insulin antiapoptotic function. Cell treatment with manumycin blocked insulin's ability to suppress pro-apoptotic caspase-3 activity which led to time-dependent proteolytic ...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi000995y

    authors: Park D,Pandey SK,Maksimova E,Kole S,Bernier M

    更新日期:2000-10-17 00:00:00

  • Phosphorylation of beta III-tubulin.

    abstract::There is considerable evidence that mammalian beta-tubulin is phosphorylated. Specifically, of the seven beta isotypes, the phosphorylated one is beta III, the isotype found almost entirely in neurons. The phosphate is added at a serine and perhaps a tyrosine near the C-terminus. All the evidence to date has been gath...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi951247p

    authors: Khan IA,Ludueña RF

    更新日期:1996-03-26 00:00:00

  • Either the carboxyl- or the amino-terminal region of the human ecto-ATPase (E-NTPDase 2) confers detergent and temperature sensitivity to the chicken ecto-ATP-diphosphohydrolase (E-NTPDase 8).

    abstract::Human ecto-ATPase (E-NTPDase 2) and chicken ecto-ATP-diphosphohydrolase (E-NTPDase 8) are cell surface nucleotidases with two transmembranous domains, one each at the N- and C-termini. Hydrolysis of substrates occurs in active sites residing in their extracellular domains. Human ecto-ATPase activity is decreased by NP...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi050019k

    authors: Mukasa T,Lee Y,Knowles AF

    更新日期:2005-08-23 00:00:00

  • Structural analysis of the catalytic mechanism and stereoselectivity in Streptomyces coelicolor alditol oxidase.

    abstract::Alditol oxidase (AldO) from Streptomyces coelicolor A3(2) is a soluble monomeric flavin-dependent oxidase that performs selective oxidation of the terminal primary hydroxyl group of several alditols. Here, we report the crystal structure of the recombinant enzyme in its native state and in complex with both six-carbon...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi701886t

    authors: Forneris F,Heuts DP,Delvecchio M,Rovida S,Fraaije MW,Mattevi A

    更新日期:2008-01-22 00:00:00