Abstract：
:According to earlier genetic experiments, a region within the N-terminal 50-100 amino acids may be important for the replication function of T antigen, the initiator protein of simian virus 40 (SV40). We have investigated this possibility using the T antigen related D2 protein in several biochemical assay systems. D2 protein, a phosphoprotein coded for by the adeno-SV40 hybrid virus Ad2+D2, shares its 594 C-terminal amino acids with authentic T antigen and its 104 N-terminal amino acids with an adenovirus structural protein. We confirmed earlier studies showing that D2 protein appeared to bind well to specific binding sites in the SV40 origin of replication. We found, however, that D2 protein was rather inefficient, inducing the unwinding of the double-stranded origin region, and was much less active than authentic T antigen as an initiator of in vitro SV40 DNA replication. We interpret these findings to indicate that D2 protein molecules associate with the origin to form an aberrant complex that is quite inefficient, inducing DNA unwinding and the establishment of replication forks. The possibility that the N-terminus may be required for an optimal arrangement of T antigen at the origin was supported by results of dephosphorylation studies. Dephosphorylation of N-terminal phosphoamino acids had significant effects on the stability of D2 protein-origin complexes.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Klausing K,Scheffner M,Scheidtmann KH,Stahl H,Knippers Rdoi
10.1021/bi00431a040subject
Has Abstractpub_date
1989-03-07 00:00:00pages
2238-44issue
5eissn
0006-2960issn
1520-4995journal_volume
28pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract：:The mammalian plasma vitamin D binding protein (DBP), or Gc-globulin, is recognized to have at least two functional properties: sterol binding and G-actin sequestration. Affinity labeling of the sterol binding site with the radioactive electrophilic ligand, 3 beta-(bromoacetoxy)-25-hydroxycholecalciferol, followed by ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00146a021
更新日期：1992-08-11 00:00:00
abstract：:Binding of the nonhydrolyzable ATP analogue 5'-adenylyl-beta, gamma-imidodiphosphate (ADPNP) to Escherichia coli DNA gyrase can lead to a limited noncatalytic supercoiling of DNA. Here we examine the efficiency of coupling between ADPNP binding and the change in linking number either of positively or negatively superc...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi952433y
更新日期：1996-02-06 00:00:00
abstract：:The ATF (activating transcription factor) binding site within the E2A promoter region of adenovirus is shown to exist in a novel conformation in vitro via nuclear magnetic resonance methods. This novel conformation may be important to the protein DNA recognition process. This conformation has characteristics of both A...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00077a003
更新日期：1993-07-06 00:00:00
abstract：:An aminoglycoside 3-acetyltransferase [AAC(3)], possibly a new isoenzymic species of the 3-N-acetyltransferase group, was purified to apparent homogeneity from a crude extract of Pseudomonas aeruginosa, a gentamicin-resistant clinical isolate. The method of purification was consecutive column chromatography--(i) gel f...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00534a009
更新日期：1982-03-02 00:00:00
abstract：:Intrastrand cross-links (IaCL) connecting two purine nucleobases in DNA pose a challenge to high-fidelity replication in the cell. Various repair pathways or polymerase bypass can cope with these lesions. The influence of the phosphodiester linkage between two neighboring 2'-deoxyguanosine (dG) residues attached throu...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.5b01078
更新日期：2015-12-29 00:00:00
abstract：:Desosamine is a 3-(dimethylamino)-3,4,6-trideoxyhexose found, for example, in such macrolide antibiotics as erthyromycin, azithromycin, and clarithromycin. The efficacies of these macrolide antibiotics are markedly reduced in the absence of desosamine. In the bacterium Streptomyces venezuelae, six enzymes are required...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi700751d
更新日期：2007-08-07 00:00:00
abstract：:The interactions of HIV-1 Env (gp120-gp41) with CD4 and coreceptors trigger a barrage of conformational changes in Env that drive the membrane fusion process. Various regions of gp41 have profound effects on HIV entry and budding. However, the precise interactions between gp41 and the membrane have not been elucidated...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi701920f
更新日期：2008-02-19 00:00:00
abstract：:The electrophoretic mobilities of 24 single-stranded DNA oligomers, each containing 26 nucleotide residues, have been measured in polyacrylamide gels and in free solution. The mobilities observed at 20 degrees C differed by approximately 20% in polyacrylamide gels and by approximately 10% in free solution, even though...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi701058f
更新日期：2007-09-25 00:00:00
abstract：:In Escherichia coli, the PurT-encoded glycinamide ribonucleotide transformylase, or PurT transformylase, catalyzes an alternative formylation of glycinamide ribonucleotide (GAR) in the de novo pathway for purine biosynthesis. On the basis of amino acid sequence analyses, it is known that the PurT transformylase belong...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi000926j
更新日期：2000-08-01 00:00:00
abstract：:L-alpha-Hydroxy acid oxidase (listed as EC 1.4.3.2, L-amino acid: O2 oxidoreductase) has been purified 100-fold from rat kidney to apparent homogeneity by gel electrophoresis. A subunit molecular weight of 47,500 was found by sodium dodecyl sulfate gel electrophoresis, but in contrast to previous reports, the enzyme h...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00683a005
更新日期：1975-06-17 00:00:00
abstract：:The aromatic residues of fd coat protein in sodium dodecyl sulfate micelles are characterized by 1H and 13C NMR. Resonances from both types of nuclei show structure-induced chemical shift dispersion and line widths indicative of a folded native structure for the protein. The two tyrosines were found to have pKas of 12...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00505a010
更新日期：1981-01-20 00:00:00
abstract：:(Z)-3-(Fluoromethyl)phosphoenolpyruvate has been synthesized in nine chemical steps from glyoxylic acid. The compound is stable at pH 3, but at pH 8 it decomposes within seconds to give 2-oxo-3-butenoate. When 3-(fluoromethyl)phosphoenolpyruvate is added to a solution of phosphoenolpyruvate carboxylase or pyruvate kin...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00404a039
更新日期：1988-02-23 00:00:00
abstract：:Perturbations within the transactivation and carboxy-terminal domains of HIV-1 Rev were examined for effects on Rev responsive element (RRE) binding activities in vitro and biological activity in vivo. Binding affinities, specificities, and multimerization of the transactivation mutants M10 and Rev/Rex M10-16 on the R...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00085a028
更新日期：1993-08-31 00:00:00
abstract：:PACT is a stress-modulated activator of interferon (IFN)-induced double-stranded (ds) RNA-activated protein kinase (PKR) and is an important regulator of PKR-dependent signaling pathways. Stress-induced phosphorylation of PACT is essential for PACT's association with PKR leading to PKR activation. PKR activation by PA...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi200104h
更新日期：2011-05-31 00:00:00
abstract：:Aromatic amino acid aminotransferase (ArAT) from Escherichia coli was overexpressed in E. coli cells, purified, and characterized. The enzyme was similar to aspartate aminotransferase (AspAT) of E. coli in many aspects, such as gross protein structure and spectroscopic properties. The reactions of pyridoxal 5'-phospha...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00096a036
更新日期：1993-11-16 00:00:00
abstract：:Two thiol-activated endopeptidases with pH optima near pH 7.5 were isolated from the supernatant fraction of rabbit brain homogenates by DEAE-cellulose chromatography, gel filtration and isoelectrofocusing. Peptide bond hydrolysis was measured quantitatively by ion-exchange chromatography with an amino acid analyzer. ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00654a026
更新日期：1976-05-04 00:00:00
abstract：:The role of ion channels in cell physiology is regulated by processes occurring after protein biosynthesis, which are critical for both channel function and targeting of channels to appropriate cell compartments. Here we apply biochemical and electrophysiological methods to investigate the role of the high-conductance...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi050527u
更新日期：2005-08-02 00:00:00
abstract：:We present a new design of mixed-backbone antisense oligonucleotides (ASOs) containing both DNA and peptide nucleic acid (PNA). Previous generations of PNA-DNA chimeras showed low binding affinity, reducing their potential as therapeutics. The addition of a 5'-wing of locked nucleic acid as well as the combination of ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.8b00827
更新日期：2019-02-12 00:00:00
abstract：:The mechanism of herpesviral protease activation upon dimerization was studied using two independent spectroscopic assays augmented by directed mutagenesis. Spectroscopic changes, attributable to dimer interface conformational plasticity, were observed upon dimerization of Kaposi's sarcoma-associated herpesvirus prote...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi011753g
更新日期：2002-02-05 00:00:00
abstract：:The rapid expansion of the amount of genomic and structural data has provided many examples of enzymes with evolutionarily related active sites that catalyze different reactions. Functional comparisons of these active sites can provide insight into the origins of the enormous catalytic proficiency of enzymes and the e...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi060847t
更新日期：2006-08-15 00:00:00
abstract：:The nuclear tRNA 3' processing activity from wheat has been characterized and partially purified. Several characteristics of the wheat nuclear 3' processing enzyme now allow this activity to be distinguished from its mitochondrial counterpart. The nuclear enzyme is an endonuclease, which we termed nuclear RNase Z. The...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi992253e
更新日期：2000-02-29 00:00:00
abstract：:The chemoreceptors of Escherichia coli are homodimeric membrane proteins that cluster in patches near the cell poles. They convert environmental stimuli into intracellular signals that control flagellar rotation. The functional domains of a receptor are physically separated by the cell membrane. Chemoeffectors bind to...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi048969d
更新日期：2005-02-01 00:00:00
abstract：:Recently, it has been shown that the activation of the Drosophila EGF receptor (DER) by its natural ligand Spitz is inhibited by Argos [Schweitzer, R., et al. (1995) Nature 376, 699-702]. Argos and Spitz both have an EGF-like domain which in the case of Argos differs from that of Spitz and other EGF receptor agonists ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi970227f
更新日期：1997-06-17 00:00:00
abstract：:The potassium form of d(CGCGAATTCGCG) solved by X-ray diffraction to 1.75 A resolution indicates that monovalent cations penetrate the primary and secondary layers of the "spine of hydration". Both the sodium [Shui, X., McFail-Isom, L., Hu, G. G., and Williams, L. D. (1998) Biochemistry 37, 8341-8355] and the potassiu...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi982063o
更新日期：1998-12-01 00:00:00
abstract：:RecG is a DNA helicase involved in the repair of damage at a replication fork and catalyzes the reversal of the fork to a point beyond the damage in the template strand. It unwinds duplex DNA in reactions that are coupled to ATP hydrolysis. The kinetic mechanism of duplex DNA unwinding by RecG was analyzed using a qua...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0512851
更新日期：2005-12-27 00:00:00
abstract：:The properties of the nucleotide binding sites in the isolated beta and alpha subunits of H(+)-ATPase from Bacillus PS3 (TF1) have been examined by studying the EPR properties of bound VO(2+), which is a paramagnetic probe for the native Mg2+ cation cofactor. The amino acid ligands of the VO2+ complexes with the isola...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi9910732
更新日期：1999-11-16 00:00:00
abstract：:Mycobacterium smegmatis MshC catalyzes the ATP-dependent condensation of GlcN-Ins and l-cysteine to form l-Cys-GlcN-Ins, the penultimate step in mycothiol biosynthesis. Attempts to crystallize the native, full-length MshC have been unsuccessful. However, incubation of the enzyme with the cysteinyl adenylate analogue, ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi801708f
更新日期：2008-12-16 00:00:00
abstract：:Stabilization of the plasma membrane Ca-ATPase (PMCA) in an inactive conformation upon oxidation of multiple methionines in the calcium regulatory protein calmodulin (CaM) is part of an adaptive cellular response to minimize ATP utilization and the generation of reactive oxygen species (ROS) under conditions of oxidat...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0474113
更新日期：2005-03-29 00:00:00
abstract：:The three-dimensional conformation of a 24-nucleotide variant of the RNA binding sequence for the coat protein of bacteriophage R17 has been analyzed using NMR, molecular dynamics, and energy minimization. The imino proton spectrum is consistent with base pairing requirements for coat protein binding known from bioche...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00019a030
更新日期：1995-05-16 00:00:00
abstract：:The flash-induced Fourier transform infrared (FTIR) difference spectrum of the oxygen-evolving Mn cluster upon S1-to-S2 transition (S2/S1 spectrum) was measured using photosystem II (PS II) core complexes of Synechocystis 6803 in which tyrosine residues were specifically labeled with 13C at the ring-4 position. The do...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi971760y
更新日期：1997-12-02 00:00:00
