Abstract：
:The role of ion channels in cell physiology is regulated by processes occurring after protein biosynthesis, which are critical for both channel function and targeting of channels to appropriate cell compartments. Here we apply biochemical and electrophysiological methods to investigate the role of the high-conductance, calcium-activated potassium (Maxi-K) channel C-terminal domain in channel tetramerization, association with the beta1 subunit, trafficking of the channel complex to the cell surface, and channel function. No evidence for channel tetramerization, cell surface expression, or function was observed with Maxi-K(1)(-)(323), a construct truncated three residues after the S(6) transmembrane domain. However, Maxi-K(1)(-)(343) and Maxi-K(1)(-)(441) are able to form tetramers and to associate with the beta1 subunit. Maxi-K(1)(-)(343)-beta1 and Maxi-K(1)(-)(441)-beta1 complexes are efficiently targeted to the cell surface and cannot be pharmacologically distinguished from full-length channels in binding experiments but do not form functional channels. Maxi-K(1)(-)(651) forms tetramers and associates with beta1; however, the complex is not present at the cell surface, but is retained intracellularly. Maxi-K(1)(-)(651) surface expression and channel function can be fully rescued after coexpression with its C-terminal complement, Maxi-K(652)(-)(1113). However coexpression of Maxi-K(1)(-)(343) and Maxi-K(1)(-)(441) with their respective C-terminal complements did not rescue channel function. Together, these data demonstrate that the domain(s) in the Maxi-K channel necessary for formation of tetramers, coassembly with the beta1 subunit, and cell surface expression resides within the S(0)-S(6) linker domain of the protein, and that structural constraints within the gating ring in the C-terminal region can regulate trafficking and function of constructs truncated in this region.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Schmalhofer WA,Sanchez M,Dai G,Dewan A,Secades L,Hanner M,Knaus HG,McManus OB,Kohler M,Kaczorowski GJ,Garcia MLdoi
10.1021/bi050527usubject
Has Abstractpub_date
2005-08-02 00:00:00pages
10135-44issue
30eissn
0006-2960issn
1520-4995journal_volume
44pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract：:The two most basic charge isomers of myelin basic protein (BP), components 1 and 2 (C1 and C2), which presumably differ in the degree of deamidation, were purified from bovine BP by cation-exchange chromatography. Two additional specific types of posttranslational modifications were introduced into the purified isomer...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00340a032
更新日期：1985-09-10 00:00:00
abstract：:The ion binding parameters of the EF-hand Ca2+ binding motif are carefully tuned for different biological applications. The present study examines the contribution of the ninth position of the Ca2+-coordinating EF-loop to the tuning of Ca2+ affinity and selectivity, using the model EF-loop of the Escherichia coli gala...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi952430l
更新日期：1996-05-28 00:00:00
abstract：:The amino acids (residues 39-51) responsible for the interaction between the first intracellular loop (iLP1) of the human prostacyclin receptor (IP) and G alpha s protein have been identified [Zhang, L., Huang, G., Wu, J., and Ruan, K. H. (2005) Biochemistry 44, 11389-11401]. To further characterize the structural/fun...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0515669
更新日期：2006-02-14 00:00:00
abstract：:Structural features of a 19-nucleotide RNA hairpin loop (5'-GGCGUACGUUUCGUACGCC-3'), a loop motif which occurs in eukaryotic 18S rRNA, have been derived using multidimensional heteronuclear NMR spectroscopy in combination with local conformational analysis and torsion angle distance geometry followed by restrained ene...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi971207g
更新日期：1997-11-18 00:00:00
abstract：:Two classes of bacteriophages, the single-stranded DNA Microviridae and the single-stranded RNA Alloleviviridae, accomplish lysis by expressing "protein antibiotics", or polypeptides that inhibit cell wall biosynthesis. Previously, we have provided genetic and physiological evidence that E, a 91-amino acid membrane pr...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi900469g
更新日期：2009-06-09 00:00:00
abstract：:The effects of nonylphenol and 3,5-dibutyl-4-hydroxytoluene (BHT) on the activity of the (Ca2(+)-Mg2+)-ATPase of skeletal muscle sarcoplasmic reticulum have been studied. At high concentrations, both inhibit the ATPase activity of the ATPase either in native lipid or in bilayers of dioleoylphosphatidylcholine but, at ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00464a028
更新日期：1990-03-27 00:00:00
abstract：:The subunit structure of Hsp80, the most abundant heat-shock protein of Neurospora crassa, was examined by chemical cross-linking of the purified protein in vitro. Resolution of glutaraldehyde-treated Hsp80 by sodium dodecyl sulfate-polyacrylamide gel electrophoresis SDS-PAGE suggests that the native state of this pro...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi963030g
更新日期：1997-08-19 00:00:00
abstract：:While the iron-molybdenum cofactor (FeMoco) of nitrogenase, a constituent of the active site for nitrogen reduction, can be extracted into N-methylformamide (NMF) and pyrrollidinone, the inability to solubilize it in any other organic solvents has hampered further understanding of its structure and chemical properties...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00450a024
更新日期：1989-11-28 00:00:00
abstract：:RuvC is a well-characterized Holliday junction resolvase from E. coli. The presence of symmetry in its preferred recognition sequence leads to ambiguity in the position of the crossover point in the junction, because a symmetric junction can undergo branch migration. Symmetric immobile junctions are junctions that con...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi020319r
更新日期：2002-09-10 00:00:00
abstract：:Using a simple model for a biological membrane we examine cation-induced gel phase formation and the depletion of polypeptide from the gel phase. The model system consists of vesicles of phosphatidic acid and phosphatidylcholine which contain gramicidin A'. By use of electron spin resonance to monitor lipid phase beha...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00282a013
更新日期：1983-06-21 00:00:00
abstract：:We examined the metabolism of exogenously added 13C-labeled fructose 1,6-bisphosphate (either labeled at the first and sixth carbons or labeled at the first carbon only) and of [2-13C]glucose in well-oxygenated and well-superfused hog carotid artery segments. Exogenously added fructose 1,6-bisphosphate was utilized by...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00004a027
更新日期：1995-01-31 00:00:00
abstract：:cis peptide bonds in proteins are often rate-limiting steps in protein folding or conformational change and are frequently stabilized by metal ions. In the collagen-binding domain of Clostridium histolyticum collagenase, the binding of calcium ions triggers the formation of a cis peptide bond. We present free energy s...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi1007047
更新日期：2010-06-29 00:00:00
abstract：:Cyclic GMP phosphodiesterase, a key enzyme in phototransduction, is composed of P alpha beta and two P gamma subunits. Interaction of P gamma with P alpha beta or with the alpha subunit (T alpha) of transducin is crucial for the regulation of cGMP phosphodiesterase in retinal photoreceptors. Here we have investigated ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi973087i
更新日期：1998-04-28 00:00:00
abstract：:Hepatic mixed-function oxidase metabolism of the ubiquitous pollutant polychlorinated biphenyls (PCBs) is implicated in their toxification and detoxification. We used dichlorobiphenyls (DCBs) as models to investigate the effect of the chloro substituent sites on this metabolism experimentally and by molecular orbital ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00529a009
更新日期：1981-12-22 00:00:00
abstract：:NADH:ubiquinone oxidoreductase (NDH-1 or complex I) from Escherichia coli was purified using a combination of anion exchange chromatography and centrifugation in sucrose density gradient. The dependence of enzyme activity on detergent and phospholipids was studied. Artificial hexaammineruthenium reductase activity was...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi050134v
更新日期：2005-06-14 00:00:00
abstract：:The three complete human LDL receptor homology regions of the LDL receptor-related protein (sLRP2, sLRP3, and sLRP4) have been expressed in Pichia pastoris SMD1168 with constitutive coexpression of the receptor-associated protein (RAP). Each sLRP was purified to homogeneity after deglycosylation using a combination of...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi034752s
更新日期：2003-11-11 00:00:00
abstract：:Amyloid deposition of human islet amyloid polypeptide (hIAPP) in the islets of Langerhans is closely associated with the pathogenesis of type II diabetes mellitus. Despite substantial evidence linking amyloidogenic hIAPP to loss of β-cell mass and decreased pancreatic function, the molecular mechanism of hIAPP cytotox...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi300542g
更新日期：2012-09-04 00:00:00
abstract：:Transcarboxylase is made up of a central hexameric subunit (S20,W similar 12 S), three peripheral dimeric metallo subunits (S20,W similar to 5 S), and six biotinyl carboxyl carrier subunits (S20,W similar to 1.3 S). The results presented here show that the carboxyl carrier subunit is required for assembly of the 12S a...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00679a010
更新日期：1975-04-22 00:00:00
abstract：:The requirement of cytochrome c-550 (PSII-V) in photosystem II (PSII) has been assessed in Synechocystis sp. PCC 6803 containing mutations between Gly-351 and Thr-436 of the loop E domain of the chlorophyll a-binding protein CP47. Six photoautotrophic strains were utilized to compare the effect of removal of either th...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi980404s
更新日期：1998-10-13 00:00:00
abstract：:Dehydroquinate synthase has been purified 9000-fold from Escherichia coli K-12 (strain MM294). The synthase is encoded by the aroB gene, which is carried by plasmid pLC29-47 from the Carbon-Clarke library. Construction of an appropriate host bearing pLC29-47 results in a strain that produces 20 times more enzyme than ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00314a036
更新日期：1984-09-11 00:00:00
abstract：:Here we explore the use of ethidium to determine relative affinities of different gene delivery vectors for DNA and describe an improved method for studying the interaction. Specifically, we investigate the binding of poly(amidoamine) dendrimers and show that the DNA-dendrimer-ethidium system is far from thermodynamic...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi1015887
更新日期：2011-02-22 00:00:00
abstract：:Regions of the negative strands of the satellite RNAs of chicory yellow mottle virus (sCYMV1) and arabis mosaic virus (sArMV) have similarity in sequence and predicted secondary structure compared to the tobacco ringspot virus satellite RNA (sTRSV) hairpin ribozyme, suggesting that they may also be catalytic RNAs of a...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00048a024
更新日期：1995-12-05 00:00:00
abstract：:The fungal neurotoxin alpha-cyclopiazonic acid (CPA), a nanomolar inhibitor of Ca(2+)-ATPase with a unique pentacyclic indole tetramic acid scaffold, is assembled by a three enzyme pathway CpaS, CpaD, and CpaO in Aspergillus sp. We recently characterized the first pathway-specific enzyme CpaS, a hybrid two module poly...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi901597j
更新日期：2009-11-24 00:00:00
abstract：:A new fluorescence method to measure viscosity in cell cytosol [Fushimi, K., & Verkman, A. S. (1991) J. Cell Biol. 112, 719-725] has been applied to determine fluid-phase viscosity in sea urchin eggs. Freshly harvested eggs from Lytechinus pictus were loaded with the dyes 2,7-bis(2-carboxyethyl)-5-(and-6-)carboxyfluor...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00115a600
更新日期：1991-12-24 00:00:00
abstract：:Previously, we have described significant effects of human insulin on glucose metabolism in the yeast Saccharomyces cerevisiae under conditions of growth limitation. These regulations apparently rely on a transmembrane receptor capable of binding human insulin and responding by tyrosine/serine phosphorylation of a spe...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi9920432
更新日期：2000-02-15 00:00:00
abstract：:The synaptotagmin (Syt) family of proteins plays an important role in vesicle docking and fusion during Ca(2+)-induced exocytosis in a wide variety of cell types. Its role as a Ca(2+) sensor derives primarily from its two C2 domains, C2A and C2B, which insert into anionic lipid membranes upon binding Ca(2+). Syt isofo...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.5b00421
更新日期：2015-09-22 00:00:00
abstract：:The three-dimensional structure of Aspergillus aculeatus beta-1,4-galactanase (AAGAL), an enzyme involved in pectin degradation, has been determined by multiple isomorphous replacement to 2.3 and 1.8 A resolution at 293 and 100 K, respectively. It represents the first known structure for a polysaccharidase with this s...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi026238c
更新日期：2002-12-24 00:00:00
abstract：:Supported phospholipid bilayers prepared by Langmuir-Blodgett techniques were introduced recently as a new model membrane system [Tamm, L.K., & McConnell, H.M. (1985) Biophys. J. 47, 105-113]. Here, supported bilayers are applied to study the lateral diffusion and lateral distribution of membrane-bound monoclonal anti...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00405a009
更新日期：1988-03-08 00:00:00
abstract：:Aldehyde dehydrogenase 7A1 (ALDH7A1) is part of lysine catabolism and catalyzes the NAD(+)-dependent oxidation of α-aminoadipate semialdehyde to α-aminoadipate. Herein, we describe a structural study of human ALDH7A1 focused on substrate recognition. Five crystal structures and small-angle X-ray scattering data are re...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.5b00754
更新日期：2015-09-08 00:00:00
abstract：:Two heat-shock proteins that show high identity with the Escherichia coli chaperonin 60 (groEL) and chaperonin 10 (groES) chaperonin proteins were purified and characterized from photolithoautotrophically grown Rhodobacter sphaeroides. The proteins were purified by using sucrose density gradient centrifugation and Mon...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00247a013
更新日期：1991-08-20 00:00:00
