Role of the C-terminus of the high-conductance calcium-activated potassium channel in channel structure and function.


:The role of ion channels in cell physiology is regulated by processes occurring after protein biosynthesis, which are critical for both channel function and targeting of channels to appropriate cell compartments. Here we apply biochemical and electrophysiological methods to investigate the role of the high-conductance, calcium-activated potassium (Maxi-K) channel C-terminal domain in channel tetramerization, association with the beta1 subunit, trafficking of the channel complex to the cell surface, and channel function. No evidence for channel tetramerization, cell surface expression, or function was observed with Maxi-K(1)(-)(323), a construct truncated three residues after the S(6) transmembrane domain. However, Maxi-K(1)(-)(343) and Maxi-K(1)(-)(441) are able to form tetramers and to associate with the beta1 subunit. Maxi-K(1)(-)(343)-beta1 and Maxi-K(1)(-)(441)-beta1 complexes are efficiently targeted to the cell surface and cannot be pharmacologically distinguished from full-length channels in binding experiments but do not form functional channels. Maxi-K(1)(-)(651) forms tetramers and associates with beta1; however, the complex is not present at the cell surface, but is retained intracellularly. Maxi-K(1)(-)(651) surface expression and channel function can be fully rescued after coexpression with its C-terminal complement, Maxi-K(652)(-)(1113). However coexpression of Maxi-K(1)(-)(343) and Maxi-K(1)(-)(441) with their respective C-terminal complements did not rescue channel function. Together, these data demonstrate that the domain(s) in the Maxi-K channel necessary for formation of tetramers, coassembly with the beta1 subunit, and cell surface expression resides within the S(0)-S(6) linker domain of the protein, and that structural constraints within the gating ring in the C-terminal region can regulate trafficking and function of constructs truncated in this region.






Schmalhofer WA,Sanchez M,Dai G,Dewan A,Secades L,Hanner M,Knaus HG,McManus OB,Kohler M,Kaczorowski GJ,Garcia ML




Has Abstract


2005-08-02 00:00:00












  • DNA intramolecular triplexes containing dT --> dU substitutions: unfolding energetics and ligand binding.

    abstract::We used a combination of optical and calorimetric techniques to investigate the incorporation of deoxythymidine --> deoxyuridine (dT --> dU) substitutions in the duplex and third strand of the parallel intramolecular triplex d(A(7)C(5)T(7)C(5)T(7)) (ATT). UV and differential scanning calorimetry melting experiments sh...


    pub_type: 杂志文章


    authors: Soto AM,Rentzeperis D,Shikiya R,Alonso M,Marky LA

    更新日期:2006-03-07 00:00:00

  • Structure and polymorphism of saturated monoacid 1,2-diacyl-sn-glycerols.

    abstract::The 1,2-diacyl-sn-glycerols (1,2-DGs) are the predominant naturally occurring isomer found in cell membranes, lipid droplets, and lipoproteins. They are involved in the metabolism of monoacylglycerols, triacylglycerols, and phospholipids. The 1,2-DGs participate in the activation of protein kinase C, in phosphorylatio...


    pub_type: 杂志文章


    authors: Kodali DR,Fahey DA,Small DM

    更新日期:1990-12-04 00:00:00

  • Nucleotides regulate the conformational state of the small terminase subunit from bacteriophage lambda: implications for the assembly of a viral genome-packaging motor.

    abstract::Terminase enzymes are responsible for "packaging" of viral DNA into a preformed procapsid. Bacteriophage lambda terminase is composed of two subunits, gpA and gpNu1, in a gpA(1).gpNu1(2) holoenzyme complex. The larger gpA subunit is responsible for preparation of viral DNA for packaging, and is central to the packagin...


    pub_type: 杂志文章


    authors: Gaussier H,Ortega ME,Maluf NK,Catalano CE

    更新日期:2005-07-19 00:00:00

  • Deep-UV resonance Raman analysis of the Rhodobacter capsulatus cytochrome bc₁complex reveals a potential marker for the transmembrane peptide backbone.

    abstract::Classical strategies for structure analysis of proteins interacting with a lipid phase typically correlate ensemble secondary structure content measurements with changes in the spectroscopic responses of localized aromatic residues or reporter molecules to map regional solvent environments. Deep-UV resonance Raman (DU...


    pub_type: 杂志文章


    authors: Halsey CM,Oshokoya OO,Jiji RD,Cooley JW

    更新日期:2011-08-02 00:00:00

  • Solution structure of the exocyclic 1,N2-propanodeoxyguanosine adduct opposite deoxyadenosine in a DNA nonamer duplex at pH 8.9. model of pH-dependent conformational transition.

    abstract::The solution structure of the complementary d(C1-A2-T3-G4-X5-G6-T7-A8-C9).d(G10-T11-A12-C13-A14-C15-A16-T17-G18) DNA duplex (designated X.A 9-mer), which contains a 1,N2-propanodeoxyguanosine exocyclic adduct X5 opposite deoxyadenosine A14 at the center, is pH dependent [Kouchakdjian, M., Eisenberg, M., Live, D., Mari...


    pub_type: 杂志文章


    authors: Huang P,Patel DJ,Eisenberg M

    更新日期:1993-04-20 00:00:00

  • Probing Structural Dynamics and Topology of the KCNE1 Membrane Protein in Lipid Bilayers via Site-Directed Spin Labeling and Electron Paramagnetic Resonance Spectroscopy.

    abstract::KCNE1 is a single transmembrane protein that modulates the function of voltage-gated potassium channels, including KCNQ1. Hereditary mutations in the genes encoding either protein can result in diseases such as congenital deafness, long QT syndrome, ventricular tachyarrhythmia, syncope, and sudden cardiac death. Despi...


    pub_type: 杂志文章


    authors: Sahu ID,Craig AF,Dunagan MM,Troxel KR,Zhang R,Meiberg AG,Harmon CN,McCarrick RM,Kroncke BM,Sanders CR,Lorigan GA

    更新日期:2015-10-20 00:00:00

  • Anomalous electrophoresis of deoxyribonucleic acid restriction fragments on polyacrylamide gels.

    abstract::A detailed study has been made of the polyacrylamide gel electrophoresis of DNA restriction fragments obtained from two plasmids, pBR322 and p82-6B. Variables studied were molecular weight, gel concentration, temperature, and electric field strength. The retardation coefficients of the larger fragments (greater than 8...


    pub_type: 杂志文章


    authors: Stellwagen NC

    更新日期:1983-12-20 00:00:00

  • A Nuclear Resonance Vibrational Spectroscopic Study of Oxy Myoglobins Reconstituted with Chemically Modified Heme Cofactors: Insights into the Fe-O2 Bonding and Internal Dynamics of the Protein.

    abstract::The molecular mechanism of O2 binding to hemoglobin (Hb) and myoglobin (Mb) is a long-standing issue in the field of bioinorganic and biophysical chemistry. The nature of Fe-O2 bond in oxy Hb and Mb had been extensively investigated by resonance Raman spectroscopy, which assigned the Fe-O2 stretching bands at ∼570 cm-...


    pub_type: 杂志文章


    authors: Ohta T,Shibata T,Kobayashi Y,Yoda Y,Ogura T,Neya S,Suzuki A,Seto M,Yamamoto Y

    更新日期:2018-12-04 00:00:00

  • Escherichia coli DNA-directed beta-galactosidase synthesis in presence and absence of Ca2+.

    abstract::DNA-dependent synthesis of beta-galactosidase was optimized in extracts made from cells lysed by a standard French pressure cell. Extracts made at 3200 psi synthesized up to 25-fold more beta-galactosidase than extracts made at 7500 psi. beta-Galactosidase synthesis was cyclic 3', 5' AMP dependent, as expected, and in...


    pub_type: 杂志文章


    authors: Jacobs KA,Schlessinger D

    更新日期:1977-03-08 00:00:00

  • Evidence for coupled motion and hydrogen tunneling of the reaction catalyzed by glutamate mutase.

    abstract::Glutamate mutase is one of a group of adenosylcobalamin-dependent enzymes that catalyze unusual isomerizations that proceed through organic radical intermediates generated by homolytic fission of the coenzyme's unique cobalt-carbon bond. These enzymes are part of a larger family of enzymes that catalyze radical chemis...


    pub_type: 杂志文章


    authors: Cheng MC,Marsh EN

    更新日期:2007-01-23 00:00:00

  • Structure-function relationship of a recombinant human galactoside-binding protein.

    abstract::A galactoside-binding lectin (hL-31) containing a collagen-like sequence was identified in human tumor cells. It was found to be the homologue of the IgE-binding protein, the macrophage cell-surface Mac-2 antigen, and the murine CBP35, RL-29, and mL-34 lectins. Here we report on the expression in Escherichia coli and ...


    pub_type: 杂志文章


    authors: Ochieng J,Platt D,Tait L,Hogan V,Raz T,Carmi P,Raz A

    更新日期:1993-04-27 00:00:00

  • Nuclear Overhauser effect as a tool for the complete assignment of nonexchangeable proton resonances in short deoxyribonucleic acid helices.

    abstract::A new strategy for the assignment of nonexchangeable proton resonances in oligonucleotide duplexes is presented and used to interpret the spectra of the oligonucleotide helix d(CpGpCpGpCpG) X d(CpGpCpGpCpG) in low salt (B form). This procedure is based on the use of sequential homodecoupling (1D) or COSY (2D) for the ...


    pub_type: 杂志文章


    authors: Frechet D,Cheng DM,Kan LS,Ts'o PO

    更新日期:1983-10-25 00:00:00

  • Interligand Overhauser effects in type II dihydrofolate reductase.

    abstract::R67 dihydrofolate reductase (DHFR) is a type II DHFR produced by bacteria as a resistance mechanism to the increased clinical use of the antibacterial drug trimethoprim. Type II DHFRs are not homologous in either sequence or structure with chromosomal DHFRs. The type II enzymes contain four identical subunits which fo...


    pub_type: 杂志文章


    authors: Li D,Levy LA,Gabel SA,Lebetkin MS,DeRose EF,Wall MJ,Howell EE,London RE

    更新日期:2001-04-10 00:00:00

  • Phosphorylation of beta III-tubulin.

    abstract::There is considerable evidence that mammalian beta-tubulin is phosphorylated. Specifically, of the seven beta isotypes, the phosphorylated one is beta III, the isotype found almost entirely in neurons. The phosphate is added at a serine and perhaps a tyrosine near the C-terminus. All the evidence to date has been gath...


    pub_type: 杂志文章


    authors: Khan IA,Ludueña RF

    更新日期:1996-03-26 00:00:00

  • The betagamma-crystallin superfamily contains a universal motif for binding calcium.

    abstract::The betagamma-crystallin superfamily consists of evolutionarily related proteins with domain topology similar to lens beta- and gamma-crystallins, formed from duplicated Greek key motifs. Ca(2+) binding was found in a few betagamma-crystallin members earlier, although its prevalence and diversity as inherent molecular...


    pub_type: 杂志文章


    authors: Aravind P,Mishra A,Suman SK,Jobby MK,Sankaranarayanan R,Sharma Y

    更新日期:2009-12-29 00:00:00

  • Catalysis at the interface: the anatomy of a conformational change in a triglyceride lipase.

    abstract::The crystal structure of an extracellular triglyceride lipase (from a fungus Rhizomucor miehei) inhibited irreversibly by diethyl p-nitrophenyl phosphate (E600) was solved by X-ray crystallographic methods and refined to a resolution of 2.65 A. The crystals are isomorphous with those of n-hexylphosphonate ethyl ester/...


    pub_type: 杂志文章


    authors: Derewenda U,Brzozowski AM,Lawson DM,Derewenda ZS

    更新日期:1992-02-11 00:00:00

  • Secondary electron transfer processes in membranes of Heliobacillus mobilis.

    abstract::Picosecond transient absorption difference spectroscopic experiments were performed on membranes of the antenna/reaction center complex of Heliobacillus mobilis to study the electron transfer processes. Particular emphasis was placed on the blue spectral region, where the difference spectra of iron-sulfur centers and ...


    pub_type: 杂志文章


    authors: Lin S,Chiou HC,Blankenship RE

    更新日期:1995-10-03 00:00:00

  • Urea-induced unfolding of Na,K-ATPase as evaluated by electron paramagnetic resonance spectroscopy.

    abstract::Urea-induced unfolding of Na,K-ATPase from pig kidney and from shark salt gland was studied by electron paramagnetic resonance (EPR) spectroscopy of a nitroxyl derivative of maleimide covalently attached to sulfhydryl groups which are essential for activity. Urea-induced structural changes lead to the inhibition of Na...


    pub_type: 杂志文章


    authors: Babavali M,Esmann M,Fedosova NU,Marsh D

    更新日期:2009-09-29 00:00:00

  • Detergent-free domain isolated from Xenopus egg plasma membrane with properties similar to those of detergent-resistant membranes.

    abstract::Microdomains known as "rafts" have been isolated from many cell types as detergent-resistant membranes (DRMs) and are enriched in sphingolipids and cholesterol. However, there has been considerable controversy over whether such domains are found in native membranes or are artificially generated by the purification pro...


    pub_type: 杂志文章


    authors: Luria A,Vegelyte-Avery V,Stith B,Tsvetkova NM,Wolkers WF,Crowe JH,Tablin F,Nuccitelli R

    更新日期:2002-11-05 00:00:00

  • Stathmin family protein SCG10 differentially regulates the plus and minus end dynamics of microtubules at steady state in vitro: implications for its role in neurite outgrowth.

    abstract::SCG10 (superior cervical ganglia neural-specific 10 protein) is a neuron specific member of the stathmin family of microtubule regulatory proteins that like stathmin can bind to soluble tubulin and depolymerize microtubules. The direct actions of SCG10 on microtubules themselves and on their dynamics have not been inv...


    pub_type: 杂志文章


    authors: Manna T,Grenningloh G,Miller HP,Wilson L

    更新日期:2007-03-20 00:00:00

  • Design and chemical synthesis of a magnetic resonance contrast agent with enhanced in vitro binding, high blood-brain barrier permeability, and in vivo targeting to Alzheimer's disease amyloid plaques.

    abstract::Molecular imaging is an important new direction in medical diagnosis; however, its success is dependent upon molecular probes that demonstrate selective tissue targeting. We report the design and chemical synthesis of a derivative of human amyloid-beta (Abeta) peptide that is capable of selectively targeting individua...


    pub_type: 杂志文章


    authors: Poduslo JF,Curran GL,Peterson JA,McCormick DJ,Fauq AH,Khan MA,Wengenack TM

    更新日期:2004-05-25 00:00:00

  • Isoforms of the EP3 subtype of human prostaglandin E2 receptor transduce both intracellular calcium and cAMP signals.

    abstract::The EP3 subtype of prostaglandin E2 receptor transduces diverse physiological responses in mammalian tissues through signaling pathways coupled to heterotrimeric G proteins. Distinct cDNA clones encoding five isoforms of the EP3 receptor were isolated from a human uterus cDNA library. The human EP3 receptor isoforms d...


    pub_type: 杂志文章


    authors: An S,Yang J,So SW,Zeng L,Goetzl EJ

    更新日期:1994-12-06 00:00:00

  • Multiple conformational changes in enzyme catalysis.

    abstract::Understanding the molecular mechanisms of enzyme catalysis and allosteric regulation has been a primary goal of biochemistry for many years. The dynamics of these processes, approached through a variety of kinetic methods, are discussed. The results obtained for many different enzymes suggest that multiple intermediat...


    pub_type: 杂志文章,评审


    authors: Hammes GG

    更新日期:2002-07-02 00:00:00

  • Primary photoreaction of photoactive yellow protein studied by subpicosecond-nanosecond spectroscopy.

    abstract::The primary photochemical event of photoactive yellow protein (PYP) was studied by laser flash photolysis experiments on a subpicosecond-nanosecond time scale. PYP was excited by a 390-nm pulse, and the transient difference absorption spectra were recorded by a multichannel spectrometer for a more reliable spectral an...


    pub_type: 杂志文章


    authors: Imamoto Y,Kataoka M,Tokunaga F,Asahi T,Masuhara H

    更新日期:2001-05-22 00:00:00

  • High-efficiency triple-helix-mediated photo-cross-linking at a targeted site within a selectable mammalian gene.

    abstract::Targeting damage to specific sites in the genome represents an attractive approach to manipulating gene function in mammalian cells. To test the applicability of triple-helix formation as a means for achieving precisely timed site-specific damage within a mammalian gene, a triplex-forming oligodeoxyribonucleotide (TFO...


    pub_type: 杂志文章


    authors: Vasquez KM,Wensel TG,Hogan ME,Wilson JH

    更新日期:1996-08-20 00:00:00

  • Evidence for partial secondary structure formation in the transition state for arc repressor refolding and dimerization.

    abstract::Structure formation and dimerization are concerted processes in the refolding of Arc repressor. The integrity of secondary structure in the transition state of Arc refolding has been investigated here by determining the changes in equilibrium stability and refolding/unfolding kinetics for a set of Ala --> Gly mutation...


    pub_type: 杂志文章


    authors: Srivastava AK,Sauer RT

    更新日期:2000-07-18 00:00:00

  • Production and characterization of bifunctional enzymes. Substrate channeling in the aspartate pathway.

    abstract::The direct channeling of an intermediate between enzymes that catalyze consecutive reactions in a pathway offers the possibility of an efficient, exclusive, and protected means of metabolite delivery. Aspartokinase-homoserine dehydrogenase I (AK-HDH I) from Escherichia coli is an unusual bifunctional enzyme in that it...


    pub_type: 杂志文章


    authors: James CL,Viola RE

    更新日期:2002-03-19 00:00:00

  • Isothermal titration calorimetric studies on the binding of deoxytrimannoside derivatives with artocarpin: implications for a deep-seated combining site in lectins.

    abstract::The carbohydrate binding specificity of the seed lectin from Artocarpus integrifolia, artocarpin, has been elucidated by the enzyme-linked lectin absorbent assay [Misquith, S., et al (1994) J. Biol. Chem. 269, 30393-30401], wherein it was demonstrated to be a Man/Glc specific lectin with high affinity for the trisacch...


    pub_type: 杂志文章


    authors: Rani PG,Bachhawat K,Reddy GB,Oscarson S,Surolia A

    更新日期:2000-09-05 00:00:00

  • Gene selection, alternative splicing, and post-translational processing regulate neuroligin selectivity for beta-neurexins.

    abstract::Neuroligins 1-4 are postsynaptic transmembrane proteins capable of initiating presynaptic maturation via interactions with beta-neurexin. Both neuroligins and beta-neurexins have alternatively spliced inserts in their extracellular domains. Using analytical ultracentrifugation, we determined that the extracellular dom...


    pub_type: 杂志文章


    authors: Comoletti D,Flynn RE,Boucard AA,Demeler B,Schirf V,Shi J,Jennings LL,Newlin HR,Südhof TC,Taylor P

    更新日期:2006-10-24 00:00:00

  • 1H NMR study of the base-pairing reactions of d(GGAATTCC): salt and polyamine effects on the imino proton exchange.

    abstract::Salts and polyamines have a variety of effects on the physical properties of DNA, including stabilization against thermal melting. We wished to gain greater insight into the mechanism of this stabilization by ascertaining its effect on the dynamics of base opening and closing reactions, as measured by NMR. Since the b...


    pub_type: 杂志文章


    authors: Braunlin WH,Bloomfield VA

    更新日期:1988-02-23 00:00:00