Solution structure of the first intracellular loop of prostacyclin receptor and implication of its interaction with the C-terminal segment of G alpha s protein.

Abstract:

:The amino acids (residues 39-51) responsible for the interaction between the first intracellular loop (iLP1) of the human prostacyclin receptor (IP) and G alpha s protein have been identified [Zhang, L., Huang, G., Wu, J., and Ruan, K. H. (2005) Biochemistry 44, 11389-11401]. To further characterize the structural/functional relationship of the iLP1 in coupling with the G alpha s protein, the solution structures of a constrained peptide (IP iLP1) that mimicked the iLP1 of the IP receptor in the absence and presence of a synthetic peptide, corresponding to the C-terminal 11 residues (Q384-L394 in the protein sequence) of the G alpha s protein (G alpha s-Ct), were determined by 2D 1H NMR spectroscopy. The NMR solution structural model of the iLP1 domain showed two turn structures in residues Arg41-Ala44 and Arg45-Phe49 with the conserved Arg45 at the center. The conformational change of the side chain of the Arg45 was observed upon the addition of the G alpha s-Ct peptide. On the other hand, the solution structural models of the G alpha s-Ct peptide in the absence and presence of the IP iLP1 peptide were also determined. The N-terminal domain (Q384-Q390 in the G alpha s protein) of the peptide adopted an alpha-helical conformation. However, the helical structure of the C-terminal domain (Q390-E392 in the G alpha s protein) of the peptide was destabilized upon addition of the IP iLP1 peptide. These structural studies have implied that there are direct or indirect contacts between the IP iLP1 domain and the C-terminal residues of the G alpha s protein in the receptor/G protein coupling. The possible charge and hydrophobic interactions between the two peptides were also discussed. These data prompted intriguing speculations on the IP/G alpha s coupling which mediates vasodilatation and inhibition of platelet aggregation.

journal_name

Biochemistry

journal_title

Biochemistry

authors

Zhang L,Wu J,Ruan KH

doi

10.1021/bi0515669

subject

Has Abstract

pub_date

2006-02-14 00:00:00

pages

1734-44

issue

6

eissn

0006-2960

issn

1520-4995

journal_volume

45

pub_type

杂志文章
  • An alpha-helical burst in the src SH3 folding pathway.

    abstract::Src SH3 is a small all-beta-sheet protein composed of a single domain. We studied the folding behavior of src SH3 at various conditions by circular dichroism (CD), fluorescence, and X-ray solution scattering methods. On the src SH3 folding pathway, an alpha-helix-rich intermediate appeared not only at subzero temperat...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi0618262

    authors: Li J,Shinjo M,Matsumura Y,Morita M,Baker D,Ikeguchi M,Kihara H

    更新日期:2007-05-01 00:00:00

  • Pressure effects and thermal stability of myosin rods and rod minifilaments: fluorescence and circular dichroism studies.

    abstract::In the present study hydrostatic pressure was applied upon both skeletal myosin rod molecules and rod minifilaments to learn more of the intra- and intermolecular interaction behavior of myosin. Applied pressure disassembled the rod minifilaments into individual rod molecules and dissociated each myosin rod molecule i...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00184a028

    authors: King L,Liu CC,Lee RF

    更新日期:1994-05-10 00:00:00

  • Beta2-microglobulin amyloid fragment organization and morphology and its comparison to Abeta suggests that amyloid aggregation pathways are sequence specific.

    abstract::Beta2-microglobulin (beta2-m) can form dialysis-related amyloid deposits. The structure of a fragment of beta2-m (K3, Ser20-Lys41) in the oligomeric state has recently been solved. We modeled equilibrium structures of K3 oligomers with different organizations (single and double layers) and morphologies (linear-like an...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi7019194

    authors: Zheng J,Jang H,Nussinov R

    更新日期:2008-02-26 00:00:00

  • Orientation of the oxygen-evolving manganese complex in a photosystem II membrane preparation: an X-ray absorption spectroscopy study.

    abstract::X-ray absorption spectroscopy has been performed on oriented photosystem II membrane particles isolated from spinach. Structural features of the tetranuclear Mn cluster and the orientation of the cluster with respect to the lipid bilayer were determined in both the S1 and S2 states of the Kok cycle. Variation of the s...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00198a042

    authors: Mukerji I,Andrews JC,DeRose VJ,Latimer MJ,Yachandra VK,Sauer K,Klein MP

    更新日期:1994-08-16 00:00:00

  • Phospholipid-binding properties of bovine factor V and factor Va.

    abstract::Factor V and factor Va binding to single bilayer phospholipid vesicles was investigated by light-scattering intensity measurements. This technique allows the measurement of free and phospholipid-bound protein concentrations from which equilibrium constants can be obtained. As controls, the Ca2+-dependent phospholipid ...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00587a023

    authors: Bloom JW,Nesheim ME,Mann KG

    更新日期:1979-10-02 00:00:00

  • Binding to cisplatin-modified DNA by the Saccharomyces cerevisiae HMGB protein Nhp6A.

    abstract::Nhp6A is an abundant non-histone chromatin-associated protein in Saccharomyces cerevisiae that contains a minor groove DNA binding motif called the HMG box. In this report, we show that Nhp6Ap binds to cisplatin intrastrand cross-links on duplex DNA with a 40-fold greater affinity than to unmodified DNA with the same ...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi012077l

    authors: Wong B,Masse JE,Yen YM,Giannikopoulos P,Feigon J,Johnson RC

    更新日期:2002-04-30 00:00:00

  • Sequence-specific assignments of the 1H nuclear magnetic resonance spectra of reduced high-potential ferredoxin (HiPIP) from Chromatium vinosum.

    abstract::The 1H resonances of the high-potential [4Fe-4S]2+ ferredoxin from Chromatium vinosum have been assigned through conventional sequential methodology applied to 2D NMR spectra. Almost 80% of the residues were identified using standard 2D COSY, HOHAHA, and NOESY pulse sequences. These residues correspond to four segment...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00139a029

    authors: Gaillard J,Albrand JP,Moulis JM,Wemmer DE

    更新日期:1992-06-23 00:00:00

  • Two disaccharides and trimethylamine N-oxide affect Abeta aggregation differently, but all attenuate oligomer-induced membrane permeability.

    abstract::Interaction between aggregates of amyloid beta protein (Abeta) and membranes has been hypothesized by many to be a key event in the mechanism of neurotoxicity associated with Alzheimer's disease (AD). Proposed membrane-related mechanisms of neurotoxicity include ion channel formation, membrane disruption, changes in m...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi9006397

    authors: Qi W,Zhang A,Good TA,Fernandez EJ

    更新日期:2009-09-22 00:00:00

  • Stopped-flow cryoenzymological investigation of the pre-steady-state kinetics of hydrolysis of Leu-Gly-NHNH-Dns by leucine aminopeptidase.

    abstract::Stopped-flow fluorescence experiments have been carried out to study the steady-state kinetics of hydrolysis of Leu-Gly-NHNH-Dns [Dns = 5-(dimethylamino)naphthalene-1-sulfonyl] by porcine kidney cytosol leucine aminopeptidase (LAP) in 50% v/v methanol/buffer solution at ambient temperature and the pre-steady-state kin...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00414a018

    authors: Lin WY,Lin SH,Morris RJ,Van Wart HE

    更新日期:1988-07-12 00:00:00

  • Cooperativity in oxidations catalyzed by cytochrome P450 3A4.

    abstract::Cytochrome P450 (P450) 3A4 is the most abundant human P450 and oxidizes a diversity of substrates, including various drugs, steroids, carcinogens, and macrolide natural products. In some reactions, positive cooperativity has been reported in microsomal studies. Flavonoids, e.g., 7,8-benzoflavone (alpha-naphthoflavone,...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi962359z

    authors: Ueng YF,Kuwabara T,Chun YJ,Guengerich FP

    更新日期:1997-01-14 00:00:00

  • Yeast alcohol dehydrogenase structure and catalysis.

    abstract::Yeast (Saccharomyces cerevisiae) alcohol dehydrogenase I (ADH1) is the constitutive enzyme that reduces acetaldehyde to ethanol during the fermentation of glucose. ADH1 is a homotetramer of subunits with 347 amino acid residues. A structure for ADH1 was determined by X-ray crystallography at 2.4 Å resolution. The asym...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi5006442

    authors: Raj SB,Ramaswamy S,Plapp BV

    更新日期:2014-09-16 00:00:00

  • Characterization of RNA aptamer binding by the Wilms' tumor suppressor protein WT1.

    abstract::The interaction of the zinc finger protein WT1 with RNA aptamers has been investigated using a quantitative binding assay, and the results have been compared to those from a previous study of the DNA binding properties of this protein. A recombinant peptide containing the four zinc fingers of WT1 (WT1-ZFP) binds to re...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi001941r

    authors: Zhai G,Iskandar M,Barilla K,Romaniuk PJ

    更新日期:2001-02-20 00:00:00

  • Investigation of quercetin binding sites on chloroplast coupling factor 1.

    abstract::The quercetin binding sites on spinach chloroplast coupling factor 1 (CF1) have been investigated using direct and competitive binding, stopped-flow, temperature-jump, and fluorescence resonance energy transfer measurements. It was found that 8-anilino-1-naphthalensulfonic acid (ANS) competes with quercetin binding at...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00646a001

    authors: Cantley LC Jr,Hammes GG

    更新日期:1976-01-13 00:00:00

  • Hydrolysis of plant cuticle by plant pathogens. Properties of cutinase I, cutinase II, and a nonspecific esterase isolated from Fusarium solani pisi.

    abstract::The properties of the homogeneous cutinase I, cutinase II, and the nonspecific esterase isolated from the extracellular fluid of cutin-grown Fusarium solani F. pisi (R.E. Purdy and P.E. Kolattukudy (1975), Biochemistry, preceding paper in this issue) were investigated. Using tritiated apple cutin as substrate, the two...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00684a007

    authors: Purdy RE,Kolattukudy PE

    更新日期:1975-07-01 00:00:00

  • Nuclear magnetic resonance study of the Schiff base in bacteriorhodopsin: counterion effects on the 15N shift anisotropy.

    abstract::High-resolution, solid-state 15N NMR has been used to study the chemical shift anisotropies of the Schiff bases in bacteriorhodopsin (bR) and in an extensive series of model compounds. Using slow-spinning techniques, we are able to obtain sufficient rotational sideband intensity to determine the full 15N chemical shif...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00434a033

    authors: de Groot HJ,Harbison GS,Herzfeld J,Griffin RG

    更新日期:1989-04-18 00:00:00

  • Slow binding inhibition of S-adenosylmethionine synthetase by imidophosphate analogues of an intermediate and product.

    abstract::S-Adenosylmethionine (AdoMet) synthetase catalyzes the only known route of biosynthesis of the primary in vivo alkylating agent. Inhibitors of this enzyme could provide useful modifiers of biological methylation and polyamine biosynthetic processes. The AdoMet synthetase catalyzed reaction converts ATP and L-methionin...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi990528k

    authors: Reczkowski RS,Markham GD

    更新日期:1999-07-13 00:00:00

  • Methyl-accepting chemotaxis proteins of Escherichia coli: methylated at three sites in a single tryptic fragment.

    abstract::The location of the sites of methylation of the methyl-accepting chemotaxis proteins (MCP) of Escherichia coli has been investigated by trypsin digestion and mapping of the peptides. The two principal MCPs, MCP I and MCP II, were found to have very similar methyl-labeled peptide patterns. Each produced three major spe...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00507a051

    authors: Chelsky D,Dahlquist FW

    更新日期:1981-02-17 00:00:00

  • Rh(phen)2phi3+ as a shape-selective probe of triple helices.

    abstract::RNA pur*pur-pyr and pyr*pur-pyr (pur = purine, pyr = pyrimidine) triple helices consisting of a Watson-Crick base-paired 28mer hairpin duplex and a Hoogsteen base-paired purine or pyrimidine 12mer are targeted with photoactivated cleavage by the metal complex Rh(phen)2phi3+ (phen = phenanthroline, phi = 9, 10-phenanth...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi980509v

    authors: Lim AC,Barton JK

    更新日期:1998-06-23 00:00:00

  • Structures of the modified folates in the thermophilic archaebacteria Pyrococcus furiosus.

    abstract::The structures of the modified folates present in Pyrococcus furiosus have been determined. This was accomplished largely by the characterization of the arylamines resulting from the air oxidative cleavage of the reduced modified folates present in these cells, using both chemical and enzymatic methods. Cell extracts ...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00054a003

    authors: White RH

    更新日期:1993-01-26 00:00:00

  • Receptor site for the 5'-phosphate of elongator tRNAs governs substrate selection by peptidyl-tRNA hydrolase.

    abstract::Eubacterial peptidyl-tRNA hydrolase (PTH) recycles all N-blocked aminoacyl-tRNA molecules but initiator formyl-methionyl-tRNAfMet, the acceptor helix of which is characterized by a 1-72 mismatch. Positive selection by PTH of noninitiator tRNA molecules with a full 1-72 base pair is abolished, however, upon the removal...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi982657r

    authors: Fromant M,Plateau P,Schmitt E,Mechulam Y,Blanquet S

    更新日期:1999-04-20 00:00:00

  • Random Mutagenesis Analysis of the Influenza A M2 Proton Channel Reveals Novel Resistance Mutants.

    abstract::The influenza M2 proton channel is a major drug target, but unfortunately, the acquisition of resistance mutations greatly reduces the functional life span of a drug in influenza treatment. New M2 inhibitors that inhibit mutant M2 channels otherwise resistant to the early adamantine-based drugs have been reported, but...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/acs.biochem.8b00722

    authors: Santner P,Martins JMDS,Kampmeyer C,Hartmann-Petersen R,Laursen JS,Stein A,Olsen CA,Arkin IT,Winther JR,Willemoës M,Lindorff-Larsen K

    更新日期:2018-10-16 00:00:00

  • Directed evolution of the suicide protein O⁶-alkylguanine-DNA alkyltransferase for increased reactivity results in an alkylated protein with exceptional stability.

    abstract::Here we present a biophysical, structural, and computational analysis of the directed evolution of the human DNA repair protein O(6)-alkylguanine-DNA alkyltransferase (hAGT) into SNAP-tag, a self-labeling protein tag. Evolution of hAGT led not only to increased protein activity but also to higher stability, especially...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi2016537

    authors: Mollwitz B,Brunk E,Schmitt S,Pojer F,Bannwarth M,Schiltz M,Rothlisberger U,Johnsson K

    更新日期:2012-02-07 00:00:00

  • X-ray crystallographic and biochemical characterization of the inhibitory action of an imidazole-dioxolane compound on heme oxygenase.

    abstract::Heme oxygenase (HO) catalyzes the regiospecific cleavage of the porphyrin ring of heme using reducing equivalents and O2 to produce biliverdin, iron, and CO. Because CO has a cytoprotective effect through the p38-MAPK pathway, HO is a potential therapeutic target in cancer. In fact, inhibition of the HO isoform HO-1 r...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi062264p

    authors: Sugishima M,Higashimoto Y,Oishi T,Takahashi H,Sakamoto H,Noguchi M,Fukuyama K

    更新日期:2007-02-20 00:00:00

  • Interactions between the mitochondrial adenosinetriphosphatase and periodate-oxidized adenosine 5'-triphosphate, an affinity label for adenosine 5'-triphosphate binding sites.

    abstract::Periodate-oxidized ATP (o-ATP) was prepared as an affinity label of nucleotide binding sites on the chloroform-released ox heart mitochondrial ATPase. In the presence of MgSO4, o-ATP is a substrate for the ATPase. It can act as a reversible, competitive inhibitor of ATPase activity and can also induce an irreversible ...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00260a025

    authors: Lowe PN,Beechey RB

    更新日期:1982-08-17 00:00:00

  • Hexachlorobenzene Catabolism Involves a Nucleophilic Aromatic Substitution and Flavin-N5-Oxide Formation.

    abstract::HcbA1 is a unique flavoenzyme that catalyzes the first step in the bacterial hexachlorobenzene catabolic pathway. Here we report in vitro reconstitution of the HcbA1-catalyzed reaction. Detailed mechanistic studies provide evidence for nucleophilic aromatic substitution and flavin-N5-oxide formation. ...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/acs.biochem.9b00012

    authors: Adak S,Begley TP

    更新日期:2019-03-05 00:00:00

  • Phenylthiazolyl-hydrazide and its derivatives are potent inhibitors of tau aggregation and toxicity in vitro and in cells.

    abstract::One of the key pathological features of Alzheimer's disease is the aggregation of tau protein. We are therefore searching for compounds capable of inhibiting this reaction. On the basis of an initial screen of 200000 compounds [Pickhardt, M., Gazova, Z., von Bergen, M., Khlistunova, I., Wang, Y., Hascher, A., Mandelko...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi700878g

    authors: Pickhardt M,Larbig G,Khlistunova I,Coksezen A,Meyer B,Mandelkow EM,Schmidt B,Mandelkow E

    更新日期:2007-09-04 00:00:00

  • pH dependence of the reduction of dioxygen to water by cytochrome c oxidase. 1. The P(R) state is a pH-dependent mixture of three intermediates, A, P, and F.

    abstract::Recent studies on cytochrome oxidase have indicated that the putative "peroxy" intermediate in the catalytic cycle (P(R)) is a mixture of intermediates, including P and F [Sucheta, A., et al. (1998) Biochemistry 37, 17905-17914], and the bench-made P and F forms appear to have the same redox state (Fe(a3)(4+)=O(2-)), ...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi020482m

    authors: Van Eps N,Szundi I,Einarsdóttir O

    更新日期:2003-05-06 00:00:00

  • Metal ion binding to dog osteocalcin studied by 1H NMR spectroscopy.

    abstract::One-dimensional 1H NMR was employed to study the effects of Ca2+ and Lu3+ binding on the apo and calcium-saturated forms of dog bone Gla protein (BGP, osteocalcin). Titration of apo dog BGP with Ca2+ in 20 mM NaCl showed spectral perturbations consistent with the binding of 5 mol equiv of calcium in the NMR slow-excha...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00093a012

    authors: Isbell DT,Du S,Schroering AG,Colombo G,Shelling JG

    更新日期:1993-10-26 00:00:00

  • Conversion of cytochrome f to a soluble form in vivo in Chlamydomonas reinhardtii.

    abstract::We introduced a stop codon in place of the ATT codon encoding Ile283 (numbered from the Met initiation codon) in the petA gene from Chlamydomonas reinhardtii. The resulting protein was expected to be truncated on its carboxy-terminus end, lacking the last 35 amino acids. This region of the polypeptide sequence encompa...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00022a021

    authors: Kuras R,Wollman FA,Joliot P

    更新日期:1995-06-06 00:00:00

  • Interactions of poly(N epsilon, N epsilon, N epsilon-trimethyllysine) and poly(N delta, N delta, N delta-trimethylornithine) with polynucleotides: salt dissociation and thermal denaturation.

    abstract::The interaction of poly(N epsilon, N epsilon, N epsilon-trimethyl-L-lysine) ([Lys(Me3)]n) and poly(N delta, N delta, N delta-trimethyl-L-ornithine) ([Orn(Me3)]n) with polynucleotides was studied by thermal denaturation, viscosity, and dissociation by salt. The methylated polymers decrease the viscosity of DNA in propo...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00555a020

    authors: Granados EN,Bello J

    更新日期:1980-07-08 00:00:00