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Using ethidium to probe nonequilibrium states of DNA condensed for gene delivery.

Abstract：

:Here we explore the use of ethidium to determine relative affinities of different gene delivery vectors for DNA and describe an improved method for studying the interaction. Specifically, we investigate the binding of poly(amidoamine) dendrimers and show that the DNA-dendrimer-ethidium system is far from thermodynamic equilibrium. Moreover, dendrimer surface modification through PEGylation appears to make the interaction with DNA more reversible, which is favorable from the perspective of vector unpacking. Probing the nonequilibrium state of DNA during condensation processes is thus important for developing novel vectors, and further, it could also be useful in the study of chromatin folding.

journal_name

Biochemistry

journal_title

Biochemistry

authors

Fant K,Nordén B,Lincoln P

doi

10.1021/bi1015887

subject

Has Abstract

pub_date

2011-02-22 00:00:00

pages

1125-7

issue

7

eissn

0006-2960

issn

1520-4995

journal_volume

50

pub_type

杂志文章

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