Abstract：
:The effect of hydrostatic pressure upon solutions of chymotrypsinogen and lysozyme at room temperature has been followed by employing a new technique [Chryssomallis, G. S., Drickamer, H. G., & Weber, G. (1978) J. Appl. Phys. 49, 3084] that permits the measurement of fluorescence polarization at pressures of up to 10 kbar. Lysozyme shows a stable, reversible 60% increase in apparent volume when the pressure is raised to 9 kbar. This can be given a simple interpretation in terms of solvent penetration of the structure at higher pressures. In contrast, the results with chymotrypsinogen are time dependent and only partially reversible on release of the pressure. They involve conversion (tl/e = 5 min) to a form with a lower rotational rate at approximately 6 kbar and return to a fast-rotating form at higher pressure. This latter form persists on pressure release. The possibility of generating what are clearly metastable conformations, not only in chymotrypsinogen but also in flavodoxins [Visser, A. J. W. G., Li, T. M., Drickamer, H. G., & Weber, G. (1977) Biochemistry 16, 4879], indicates that there are unresolved questions about the relative stability of protein conformations which can be profitably investigated by high-pressure experiments.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Chryssomallis GS,Torgerson PM,Drickamer HG,Weber Gdoi
10.1021/bi00517a002subject
Has Abstractpub_date
1981-07-07 00:00:00pages
3955-9issue
14eissn
0006-2960issn
1520-4995journal_volume
20pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract：:Two bands of 5.8S rRNA were observed when the total RNA isolated from rat or mouse tissue was separated by electrophoresis on high-resolution polyacrylamide gels under denaturing conditions. The minor form, with a lower mobility, represented 15-35% of the total 5.8S rRNA, depending on the source of the tissue. Sequenc...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00311a011
更新日期：1984-07-31 00:00:00
abstract：:The data and approach reported in paper I (Hanlon et al., 1975, preceding paper) have been used to calculate the fractional changes in secondary structure of calf thymus deoxyribonucleic acid which occur in aqueous solutions as a function of the concentration of NaCl, KCl, LiCl, CsCl, and NH4Cl. There is a continuous ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00679a018
更新日期：1975-04-22 00:00:00
abstract：:The enthalpies of transfer of hen egg white lysozyme from water to aqueous solutions of 1-propanol were determined by isothermal flow calorimetry at 10, 17, 25, and 40 degrees C in 0.04 M, pH 2 glycine buffer. Alcohol concentrations up to 3.4 M were employed. Four regions in the dependence of the enthalpy of transfer ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00514a016
更新日期：1981-05-26 00:00:00
abstract：:The allosteric activation of the T127-->L mutant of 3',5'-cyclic adenosine monophosphate (cAMP) receptor protein (CRP) by cAMP changes from an exothermic, independent two-site binding mechanism at pH 7.0 to an endothermic, interacting two-site binding mechanism at pH 5.2, similar to that observed for CRP at pH 7.0 and...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi000225m
更新日期：2000-06-20 00:00:00
abstract：:The 86-amino acid colicin E9 immunity protein (Im9), which inhibits the DNase activity of colicin E9, has been overexpressed in Escherichia coli and isotopically enriched with 15N and 13C. Using the 3D CBCANH and CBCA(CO)NH experiments, we have almost completely assigned the backbone 13C resonances and extended previo...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi960401k
更新日期：1996-07-23 00:00:00
abstract：:A series of cysteine-bearing hydrophobic polypeptides analogous to a light-harvesting one betapolypeptide (LH1beta) from the LH1 complex from the purple photosynthetic bacterium, Rhodobacter sphaeroides, was synthesized using an Escherichia coli expression system. The cysteine was placed in the C- or N-terminal region...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0478493
更新日期：2005-04-05 00:00:00
abstract：:Lecithin retinol acyltransferase (LRAT) catalyzes the esterification of all-trans-retinol into all-trans-retinyl ester, an essential reaction in the vertebrate visual cycle. Since all-trans-retinyl esters are the substrates for the isomerization reaction that generates 11-cis-retinoids, this esterification reaction is...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0342416
更新日期：2003-05-27 00:00:00
abstract：:Iron is vital for almost all organisms because of its ability to donate and accept electrons with relative ease. It serves as a cofactor for many proteins and enzymes necessary for oxygen and energy metabolism, as well as for several other essential processes. Mammalian cells utilize multiple mechanisms to acquire iro...
journal_title：Biochemistry
pub_type： 杂志文章,评审
doi：10.1021/bi300752r
更新日期：2012-07-24 00:00:00
abstract：:Amyloid formation by immunoglobulin light chain (LC) proteins is associated with amyloid light chain (AL) amyloidosis. Destabilization of the native state of the variable domain of the LC (VL) is known to be one of the critical factors in promoting the formation of amyloid fibrils. However, determining the key residue...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi5007892
更新日期：2014-08-12 00:00:00
abstract：:A critical event in Alzheimer's disease is the transition of Abeta peptides from their soluble forms into disease-associated beta-sheet-rich conformers. Structural analysis of a complete D-amino acid replacement set of Abeta(1-42) enabled us to localize in the full-length 42-mer peptide the region responsible for the ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi002005e
更新日期：2001-05-08 00:00:00
abstract：:The formation and role of arachidonic acid (AA) and its metabolites during gonadotropin releasing hormone- (GnRH-) induced gonadotropin secretion were investigated in primary cultures of rat pituitary cells. Prelabeled cells ([3H]AA) responded to GnRH challenge with increased formation (about 2-fold) of the leukotrien...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00139a004
更新日期：1992-06-23 00:00:00
abstract：:Polyalanine (polyA) is the third-most prevalent homopeptide repeat in eukaryotes, behind polyglutamine and polyasparagine. Abnormal expansion of the polyA repeat is linked to at least nine human diseases, and the disease mechanism likely involves enhanced length-dependent aggregation. Because of the simplicity of its ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi201155g
更新日期：2011-11-01 00:00:00
abstract：:Vitamin B12-dependent methionine synthase catalyzes the transfer of a methyl group from methyltetrahydrofolate to homocysteine via the enzyme-bound cofactor methylcobalamin. To carry out this reaction, the enzyme must alternately stabilize six-coordinate methylcobalamin and four-coordinate cob(I)alamin oxidation state...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi952389m
更新日期：1996-02-20 00:00:00
abstract：:Isotope exchange studies show that beef liver fructokinase has a random kinetic mechanism in which release of fructose from the enzyme is slower than that catalytic reaction. The stickiness of fructose in the presence of MgATP is confirmed by isotope partition studies, which show it to be released 0.53 times as fast a...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00629a021
更新日期：1977-05-17 00:00:00
abstract：:Actin-depolymerizing factor (ADF)/cofilin enhances the turnover of actin filaments by two separable activities: filament severing and pointed-end depolymerization. Multicellular organisms express multiple ADF/cofilin isoforms in a tissue-specific manner, and the vertebrate proteins are grouped into ADFs and cofilins o...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi050933d
更新日期：2005-11-01 00:00:00
abstract：:The structural consequences of specific base sequences in DNA can exert a strong influence on the function of DNA. It has previously been reported that the presence of multiple B-Z conformational junctions in constructed DNA oligomers results in unusually enhanced electrophoretic gel mobilities of these oligomers [Win...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00134a026
更新日期：1992-05-19 00:00:00
abstract：:Group II introns self-splice via a two-step mechanism: cleavage at the 5' splice site followed by exon ligation at the 3' splice site. The second step has been difficult to study in vitro because it is generally faster than the first. Herein we describe development and partial kinetic characterization of a novel assay...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi000808w
更新日期：2000-08-22 00:00:00
abstract：:The global transcription factor, p53, is a master regulator of gene expression in cells. Mutations in the TP53 gene promote unregulated cell growth through the inactivation of downstream effectors of the p53 pathway. In fact, mutant p53 is highly prone to misfolding and frequently resides inside the cell as large aggr...
journal_title：Biochemistry
pub_type： 杂志文章,评审
doi：10.1021/acs.biochem.9b00796
更新日期：2020-01-21 00:00:00
abstract：:The structure of the dimeric form of the protein L7/L12 from ribosomes from Escherichia coli was studied by using the heterobifunctional cross-linker 4-(6-formyl-3-azidophenoxy)butyrimidate. The imidate group of the cross-linker reacts very specifically with Lys-51 of L7/L12. Subsequent cross-linking of this modified ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00507a057
更新日期：1981-02-17 00:00:00
abstract：:Recent results on the 102 residue protein U1A show that protein aggregation is not always slow and irreversible but may take place transiently in refolding studies on a millisecond time scale. In this study we observe a similar aggregation behavior with the classical two-state protein CI2. Since both U1A and CI2 appea...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi9909997
更新日期：1999-10-05 00:00:00
abstract：:In this work, we show that adenosine 5'-O-(3-thiotriphosphate) (ATP gamma S) is a substrate for yeast inorganic pyrophosphatase (PPase) (EC 3.6.1.1) and further, using chirally labeled [gamma-17O,18O]ATP gamma S, that enzyme-catalyzed hydrolysis to produce chiral inorganic thio[17O,18O]phosphate proceeds with inversio...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00300a002
更新日期：1984-02-28 00:00:00
abstract：:The interaction of the monofunctional chorismate mutase from Bacillus subtilis with chorismate and prephenate has been studied kinetically and by NMR spectroscopy with 13C specifically labeled substrates. Prephenate dominates the population of enzyme-bound species, and the "off" rate constant (approximately 60 s-1) ob...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00489a051
更新日期：1990-09-18 00:00:00
abstract：:Mammalian type B (mitochondrial) b(5) cytochromes exhibit greater amino acid sequence diversity than their type A (microsomal) counterparts, as exemplified by the type B proteins from human (hCYB5B) and rat (rCYB5B). The comparison of X-ray crystal structures of hCYB5B and rCYB5B reported herein reveals a striking dif...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi2004729
更新日期：2011-06-21 00:00:00
abstract：:The assignment of the 1H, 15N, 13CO, and 13C resonances of recombinant human interleukin-4 (IL-4), a protein of 133 residues and molecular mass of 15.4 kDa, is presented based on a series of 11 three-dimensional (3D) double- and triple-resonance heteronuclear NMR experiments. These studies employ uniformly labeled 15N...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00132a026
更新日期：1992-05-05 00:00:00
abstract：:Bicyclomycin is a novel, commercially important antibiotic. Information concerning the site of bicyclomycin inhibition in Escherichia coli has been obtained by the production of bicyclomycin resistant mutants by UV irradiation. Selection by growth in the presence of bicyclomycin of a plasmid clone library generated fr...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00065a007
更新日期：1993-04-13 00:00:00
abstract：:The DNA repair enzyme MutY plays an important role in the prevention of DNA mutations resulting from the presence of the oxidatively damaged lesion 7,8-dihydro-8-oxo-2'-deoxyguanosine (OG) in DNA by the removal of misincorporated adenine residues in OG:A mispairs. MutY also exhibits adenine glycosylase activity toward...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi981594+
更新日期：1998-10-20 00:00:00
abstract：:The covalent affinity probe maltosyl isothiocyanate (MITC) has been used previously to identify the glucose transporter of human erythrocytes as a component of band 3. By use of limited proteolysis, the site on the Mr 100 000 protein to which MITC attaches has been localized to a 17 000-dalton region near the center o...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00331a001
更新日期：1985-05-07 00:00:00
abstract：:The mechanisms of interaction of the recombinant N-terminal portion of bactericidal/permeability-increasing protein, rBPI21, with various planar asymmetric and symmetric bilayer membranes, including the lipid matrix of the outer membrane of Gram-negative bacteria, were investigated via electrical measurements. For the...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi970177e
更新日期：1997-08-19 00:00:00
abstract：:We have labeled the primer binding domain of murine leukemia virus reverse transcriptase (MuLV RT) by covalently cross-linking 5' end labeled d(T)8 to MuLV RT, using ultraviolet light energy. The specificity and the functional significance of the primer cross-linking reaction were demonstrated by the fact that (i) oth...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00240a014
更新日期：1991-07-02 00:00:00
abstract：:Photoaffinity labeling of the nicotinic acetylcholine receptor from Torpedo marmorata electric tissue was performed in the presence of cholinergic effectors in the millisecond to second time range by a combination of a stopped-flow apparatus and a high-energy pulse laser. The label applied was [3H]triphenylmethylphosp...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00307a029
更新日期：1984-06-05 00:00:00
