Abstract：
:Two bands of 5.8S rRNA were observed when the total RNA isolated from rat or mouse tissue was separated by electrophoresis on high-resolution polyacrylamide gels under denaturing conditions. The minor form, with a lower mobility, represented 15-35% of the total 5.8S rRNA, depending on the source of the tissue. Sequence analysis and the kinetics of formation showed that this minor form is elongated at the 5' end and is not a precursor. The sequence of the minor form was found to be p(C)CGAUA[CG-, five or six nucleotides longer than the major form. The minor 5.8S rRNA constituent also formed a more stable junction complex with 28S rRNA than the shorter major sequence. The rat DNA sequence that corresponds to the additional nucleotides at the 5' end of 5.8S rRNA has been reported to be -CCGTACG-[Subrahmanyam, C. S., Cassidy, B., Busch, H., & Rothblum, L. I. (1982) Nucleic Acids Res. 10, 3667-3680], a sequence which does not contain the extra adenylic acid residue at position 4 found in the minor form. This suggests that the rodent rRNA genes are heterogeneous and that the insertion of an A residue in the ribosomal precursor RNA can generate an alternate processing site.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Smith SD,Banerjee N,Sitz TOdoi
10.1021/bi00311a011subject
Has Abstractpub_date
1984-07-31 00:00:00pages
3648-52issue
16eissn
0006-2960issn
1520-4995journal_volume
23pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract：:To understand the stabilization, folding, and functional mechanisms of proteins, it is very important to understand the structural and thermodynamic properties of the molten globule state. In this study, the global structure of the acid molten globule state, which we call MG1, of horse cytochrome c at low pH and high ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi101806b
更新日期：2011-04-19 00:00:00
abstract：:Despite many studies suggesting the role of enolpyruvate as a bound intermediate in the pyruvate kinase reaction, direct evidence for it has been lacking. By use of a combination of chemical trapping and isolation of a derivative, significant amounts of enzyme-bound enolpyruvate have now been demonstrated. The method ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00217a022
更新日期：1991-01-22 00:00:00
abstract：:The HAMP domain is a conserved motif widely distributed in prokaryotic and lower eukaryotic organisms, where it is often found in transmembrane receptors that regulate two-component signaling pathways. The motif links receptor input and output modules and is essential to receptor structure and signal transduction. Rec...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi701832b
更新日期：2007-12-04 00:00:00
abstract：:14C-Labeled fluorodinitrobenzene and N-ethylmaleimide have been used as chemical probes of the conformational states of myosin induced by the binding of MgADP and MgATP. The results indicate that in the high-energy conformation, MMgADP-Pi, the essential thiols are protected from modification but their diminished react...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00643a005
更新日期：1977-11-29 00:00:00
abstract：:The potency of cholesterol to affect the acyl chain order and dynamics of cardiolipin membranes in the liquid-crystalline state was monitored by steady-state and time-resolved fluorescence anisotropy as well as excited-state lifetime measurements with cis- and trans-parinaric acids as probes. Up to a cholesterol mole ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00357a054
更新日期：1986-05-06 00:00:00
abstract：:The effect of concentrated glycerol on the thermal transitions of chymotrypsinogen and ribonuclease has been examined by differential spectrophotometry at 293 and 287 mm, respectively. It was found that for both proteins addition of glycerol raises the transition temperature, the increase in Tm being greater for ribon...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00519a024
更新日期：1981-08-04 00:00:00
abstract：:The organization and interaction of chlorophylls (Chl) and the kinetics of the energy transfer in the core antenna of photosystem I (PSI) trimeric and monomeric complexes, isolated from Spirulina platensis with Triton X-100 have been studied by stationary and time-resolved fluorescence. At 295 K both complexes show an...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi970386z
更新日期：1997-11-11 00:00:00
abstract：:We have used DNase I footprinting to examine the formation of antiparallel DNA triple helices on DNA fragments containing the homopurine target sites (GGA)2GGX(GGA)2GG.(CCT)2CCZ(CCT)2CC (where X.Z is each base pair in turn), with the GA- and GT-rich oligonucleotides, (GGA)2GGN(GGA)2GG and (GGT)2GGN(GGT)2GG (N = each b...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi9609679
更新日期：1996-11-26 00:00:00
abstract：:The intracellular level of beta-hydroxydecanoyl thio ester dehydrase, the product of the fabA gene of Escherichia coli, was increased by isolation of a putative promotor mutant (termed fabAup) or by molecular cloning of the wild-type fabA gene into plasmid pBR322. The fabAup and plasmid-carrying strains overproduced d...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00294a032
更新日期：1983-12-06 00:00:00
abstract：:We have studied the structure of the CuB site in the binuclear heme-copper center of the fully oxidized form of the quinol-oxidizing cytochrome aa3-600 from Bacillus subtilis by EXAFS and ENDOR spectroscopy. This enzyme is member of the large superfamily of heme-copper respiratory oxidases, which catalyze the reductio...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00032a019
更新日期：1995-08-15 00:00:00
abstract：:The three-dimensional structure of d[CGTA'CG], where A' = [2-NH2-A], was determined to atomic (1.35 A) resolution by single isomorphous replacement. The d[CGTA'CG] hexamer crystallizes in space group P3221, and is not isomorphous with other DNA hexanucleotides. Despite completely different crystal packing, the essenti...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00047a014
更新日期：1995-11-28 00:00:00
abstract：:The left-handed polyproline II (P(II)) helix is a structure that has been given a great deal of attention lately because of its role in a wide variety of physiologically important processes and potential significance in protein unfolded states. Recent work by several authors has shown that residues besides proline can...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi049922v
更新日期：2004-05-18 00:00:00
abstract：:The three-dimensional conformation of a 24-nucleotide variant of the RNA binding sequence for the coat protein of bacteriophage R17 has been analyzed using NMR, molecular dynamics, and energy minimization. The imino proton spectrum is consistent with base pairing requirements for coat protein binding known from bioche...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00019a030
更新日期：1995-05-16 00:00:00
abstract：:We have mapped the interface between the two transmembrane alpha-helices in the membrane domain of the Escherichia coli enzyme leader peptidase by analyzing disulfides formed between pairs of cysteine residues introduced near their respective periplasmic ends. The interface is formed primarily from aliphatic amino aci...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00084a020
更新日期：1993-08-24 00:00:00
abstract：:Restrictocin is a small basic protein produced by the fungus Aspergillus restrictus. It potently inhibits protein synthesis in eukaryotic cells by specifically cleaving a single phosphodiester bond in 28S rRNA. A histidine residue at position 49 in restrictocin has been implicated in its active site. A mutant of restr...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi971177h
更新日期：1997-11-04 00:00:00
abstract：:Human genomic clones that span the entire protein S expressed gene (PS alpha) and the 3' two-thirds of the protein S pseudogene (PS beta) have been isolated and characterized. The PS alpha gene is greater than 80 kilobases in length and contains 14 introns and 15 exons, as well as 6 repetitive "Alu" sequences. Exons I...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00486a010
更新日期：1990-08-28 00:00:00
abstract：:1H NMR spectroscopy of the isotropically shifted signals in cobalt carboxypeptidase, CoCPD, permits a direct and selective detection of protons belonging to the residues liganded to the metal. The chemical shift of these protons in the free enzyme and enzyme-inhibitor complexes with changing pH monitors the state of i...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00130a015
更新日期：1992-04-21 00:00:00
abstract：:We have investigated the kinetic and thermodynamic properties of carbon monoxide binding to the fully reduced quinol oxidase (cytochrome aa(3)) from the hyperthermophilic archaeon Acidianus ambivalens. After flash photolysis of CO from heme a(3), the complex recombines with an apparent rate constant of approximately 3...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi990473m
更新日期：1999-08-03 00:00:00
abstract：:Colchicine induces a weak assembly-independent GTPase activity in calf brain tubulin [David-Pfeuty, T., Erickson, H. P., & Pantaloni, D. (1977) Proc. Natl. Acad. Sci. U.S.A. 74, 5372-5376; Andreu, J. M., & Timasheff, S. N. (1981) Arch. Biochem. Biophys. 211, 151-157]. Kinetic analysis shows a turnover number of 2 x 10...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00186a027
更新日期：1994-05-24 00:00:00
abstract：:Glycophorin A is a sialoglycoprotein isolated from human erythrocyte membranes which seems to exist as stable dimeric complexes in the presence of sodium dodecyl sulfate. When analyzed by dodecyl sulfate acrylamide electrophoresis this molecule forms two PAS-stainable bands (PAS-U and PAS-2) which are reversibly inter...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00650a028
更新日期：1976-03-09 00:00:00
abstract：:An organic solvent soluble polypeptide has been isolated from photoreceptor complexes and chromatophores of Rhodospirillum rubrum. After extraction of the protein from lyophilized samples with 1:1 chloroform-methanol, it was purified by column chromatography. Its isoelectric point determined by isoelectric focusing wa...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00624a011
更新日期：1977-03-08 00:00:00
abstract：:Decorin-binding protein A (DBPA) is an important lipoprotein from the bacterium Borrelia burgdorferi, the causative agent of Lyme disease. The absence of DBPA drastically reduces the pathogenic potential of the bacterium, and biochemical evidence indicates DBPA's interactions with the glycosaminoglycan (GAG) portion o...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi3007093
更新日期：2012-10-23 00:00:00
abstract：:Cytochrome P450 158A2 (CYP158A2) has been shown to catalyze an unusual oxidative C-C coupling reaction to polymerize flaviolin and form highly conjugated pigments (three isomers of biflaviolin and one triflaviolin) in Streptomyces coelicolor A3(2) which protect the soil bacterium from deleterious effects of UV irradia...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi7006959
更新日期：2007-07-31 00:00:00
abstract：:Deoxycytidine kinase (dCK) uses either ATP or UTP as a phosphoryl donor to catalyze the phosphorylation of nucleoside acceptors. The kinetic properties of human dCK are modulated in vivo by phosphorylation of serine 74. This residue is a part of the insert region and is distant from the active site. Replacing the seri...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi2001032
更新日期：2011-04-12 00:00:00
abstract：:A set of peptides derived from the N-terminal domain of the ribosomal protein L9 (NTL9) have been characterized in an effort to define the minimum unit of this domain required to fold and to provide model peptides for the analysis of electrostatic interactions in the unfolded state. NTL9 is a 56-residue alpha-beta pro...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi026410c
更新日期：2002-11-12 00:00:00
abstract：:Previous studies of recombinant full-length human apolipoprotein A-V (apoA-V) provided evidence of the presence of two independently folded structural domains. Computer-assisted sequence analysis and limited proteolysis studies identified an N-terminal fragment as a candidate for one of the domains. C-Terminal truncat...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi800515c
更新日期：2008-08-19 00:00:00
abstract：:The first step in sulfur mobilization for the biosynthesis of Fe-S clusters under oxidative stress and iron starvation in Escherichia coli involves a cysteine desulfurase SufS. Its catalytic reactivity is dependent on the presence of a sulfur acceptor protein, SufE, which acts as the preferred substrate for this enzym...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi4001479
更新日期：2013-06-11 00:00:00
abstract：:Phosphorylation of the sarcoplasmic reticulum calcium ATPase, E, is first order with kb = 70 +/- 7 s-1 after free enzyme was mixed with saturating ATP and 50 microM Ca2+; this is one-third the rate constant of 220 s-1 for phosphorylation of enzyme preincubated with calcium, cE.Ca2, after being mixed with ATP under the...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00398a019
更新日期：1987-12-01 00:00:00
abstract：:Several basic proteins, including the ubiquitous HU proteins, serve histone-like functions in prokaryotes. Significant sequence conservation exists between HU homologues; yet binding sites varying from 9 to 37 bp have been reported. TF1, an HU homologue with a 37 bp binding site that is encoded by the Bacillus subtili...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi016095e
更新日期：2002-06-18 00:00:00
abstract：:X-ray solution scattering data from skeletal muscle troponin C and from calmodulin have been measured. Modeling studies based on the crystal structure coordinates for these proteins show discrepancies between the solution data and the crystal structure that indicate that if the size and shape of the globular domains a...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00403a011
更新日期：1988-02-09 00:00:00
