Abstract：
:Recent results on the 102 residue protein U1A show that protein aggregation is not always slow and irreversible but may take place transiently in refolding studies on a millisecond time scale. In this study we observe a similar aggregation behavior with the classical two-state protein CI2. Since both U1A and CI2 appear to fold directly from the coil at low protein concentrations, it is likely that the aggregates also form directly from the coil. This is in contrast to the behavior of larger multistate proteins where aggregation occurs in connection to "sticky" intermediates.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Silow M,Tan YJ,Fersht AR,Oliveberg Mdoi
10.1021/bi9909997subject
Has Abstractpub_date
1999-10-05 00:00:00pages
13006-12issue
40eissn
0006-2960issn
1520-4995pii
bi9909997journal_volume
38pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract：:The effect of pretreatment with sodium butyrate on DNA excision repair was studied in intact and permeable confluent (i.e., growth-inhibited) diploid human fibroblasts. Exposure to 20 mM sodium butyrate for 48 h increased subsequent ultraviolet (UV)-induced [methyl-3H]thymidine incorporation by intact AG1518 fibroblas...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00345a019
更新日期：1985-11-19 00:00:00
abstract：:Two distinct regions of angiogenin are critical for angiogenic activity: a catalytic site capable of cleaving RNA and a noncatalytic site, encompassing residues 60-68, which may bind to an endothelial cell-surface receptor [Hallahan, T. W., Shapiro, R., & Vallee, B. L. (1991) Proc. Natl. Acad. Sci. U.S.A. 88, 2222-222...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00149a036
更新日期：1992-09-01 00:00:00
abstract：:Adenylosuccinate lyase, an enzyme catalyzing two reactions in purine biosynthesis (the cleavage of either adenylosuccinate or succinylaminoimidazole carboxamide ribotide), has been implicated in a human disease arising from point mutations in the gene encoding the enzyme. Asn(276) of Bacillus subtilis adenylosuccinate...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi020640+
更新日期：2003-02-25 00:00:00
abstract：:We present spectroscopic evidence for the presence of low-barrier hydrogen bonds (LBHBs) in molecular complexes composed of carboxylic acids and 1-methylimidazole (1-MeIm) dissolved in aprotic organic solvents. A plot of the values of the low-field proton NMR chemical shifts versus the aqueous pKa of the carboxylic ac...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00021a002
更新日期：1995-05-30 00:00:00
abstract：:A molecular model has been built of the equine infectious anemia virus (EIAV) proteinase on the basis of the crystal structures of the related Rous sarcoma virus (RSV) and human immunodeficiency virus (HIV) proteinases. The 104 residue long EIAV proteinase has 30 identical and 11 similar amino acids compared to those ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00064a019
更新日期：1993-04-06 00:00:00
abstract：:Trp151 in the lactose permease of Escherichia coli (LacY) is an important component of the sugar-binding site and the only Trp residue out of six that is in close proximity to the galactopyranoside in the structure (1PV7). The short distance between Trp151 and the sugar is favorable for Förster resonance energy transf...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi061632m
更新日期：2006-12-26 00:00:00
abstract：:The reduction of substrates catalyzed by nitrogenase utilizes an electron transfer (ET) chain comprised of three metalloclusters distributed between the two component proteins, designated as the Fe protein and the MoFe protein. The flow of electrons through these three metalloclusters involves ET from the [4Fe-4S] clu...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi201003a
更新日期：2011-11-01 00:00:00
abstract：:The solution structure of the self-complementary dodecamer 5'd(CGCGPATTCGCG)2, containing a purine-thymine base pair within the hexameric canonical recognition site GAATTC for the restriction endonuclease EcoRI, is investigated by nuclear magnetic resonance spectroscopy and restrained molecular dynamics. Nonexchangeab...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00411a042
更新日期：1988-05-31 00:00:00
abstract：:Proelastase has been purified to homogeneity from rat pancreatic tissue by a combination of CM-Sephadex and immobilized protease inhibitor affinity resins. Trypsin activation yields an elastolytic enzyme that possesses a specificity toward small hydrophobic residues in synthetic amide substrates, similar to those of p...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00285a008
更新日期：1983-08-02 00:00:00
abstract：:Cu,Zn superoxide dismutase from Photobacterium leiognathi has been cloned and expressed in Escherichia coli. The circular dichroism spectrum in the UV region of the recombinant protein indicates an higher content of random coil structure with respect to the eukaryotic enzymes. Investigation of the active site by optic...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi963020f
更新日期：1997-06-10 00:00:00
abstract：:Amyloid-beta (Abeta) peptide deposition as fibrillar senile plaques is a key element in the pathology of Alzheimer's disease. Here we present a high-resolution structure of an Abeta amyloid fibril using magnetically aligned preparations of a central Abeta domain which forms representative amyloid fibrils. Diffraction ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi000637v
更新日期：2000-10-31 00:00:00
abstract：:p-Cresol methylhydroxylase (PCMH) isolated from Pseudomonas putida is an alpha 2 beta 2 tetramer of approximate subunit Mr 49,000 and 9,000. It is a flavocytochrome c containing covalently bound FAD in the larger subunit and covalently bound heme in the smaller. Crystals in space group P2(1)2(1)2(1) with unit-cell par...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00215a034
更新日期：1991-01-08 00:00:00
abstract：:Lysyl-5-fluoro-L-tryptophyllysine and lysyl-5-fluoro-D-tryptophyllysine were synthesized, and their interactions with double-stranded DNA were investigated as a model for protein-nucleic acid interactions. The binding to DNA was studied by monitoring various 19F NMR parameters, the fluorescence, and the optical absorb...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00337a012
更新日期：1985-07-30 00:00:00
abstract：:Acetylcholine receptors have been purified from junctional regions of normal rat diaphragm muscle and from extrajunctional regions of denervated diaphragm. The reaction of purified receptors with [122I]-alpha-bungarotoxin has been investigated by kinetic methods. The toxin-receptor complexes dissociated in a biphasic ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00681a008
更新日期：1975-05-20 00:00:00
abstract：:The intracellular level of beta-hydroxydecanoyl thio ester dehydrase, the product of the fabA gene of Escherichia coli, was increased by isolation of a putative promotor mutant (termed fabAup) or by molecular cloning of the wild-type fabA gene into plasmid pBR322. The fabAup and plasmid-carrying strains overproduced d...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00294a032
更新日期：1983-12-06 00:00:00
abstract：:Hormone-sensitive lipase (HSL) is a rate-limiting enzyme in lipolysis that displays broad substrate specificity. HSL function is regulated by reversible phosphorylation that occurs within a 150 aa "regulatory module" of the protein. The current studies used mutational analysis to dissect the contribution of the "regul...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi049206t
更新日期：2005-02-15 00:00:00
abstract：:The proton nuclear Overhauser effects of bovine alpha-lactalbumin were studied at 200 MHz by irradiation of an upfield ring current shifted methylene at -2.45 ppm (assigned to Ile-95) and two aromatic protons, Tyr-103 (8.36 ppm) and Trp-60 (5.85 ppm). The experimental results were consistent with a putative three-dime...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00346a035
更新日期：1985-12-03 00:00:00
abstract：:The tRNA(His) guanylyltransferase (Thg1) catalyzes the incorporation of a single guanosine residue at the -1 position (G(-1)) of tRNA(His), using an unusual 3'-5' nucleotidyl transfer reaction. Thg1 and Thg1 orthologs known as Thg1-like proteins (TLPs), which catalyze tRNA repair and editing, are the only known enzyme...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi201397f
更新日期：2012-01-10 00:00:00
abstract：:Telomerase is a ribonucleoprotein that participates in the maintenance of telomere length. Its activity is up-regulated in many tumor types, suggesting that it may be a novel target for chemotherapy. The RNA component of telomerase contains an active site that plays at least two roles&sbd;binding telomere ends and tem...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi970438k
更新日期：1997-09-30 00:00:00
abstract：:We have carried out a comparative study of the effect of cholesterol on the thermotropic phase behavior of the distearoyl and dielaidoyl molecular species of phosphatidylcholine, phosphatidylethanolamine, and phosphatidylserine using high-sensitivity differential scanning calorimetry. For both molecular species of pho...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi962815j
更新日期：1997-04-22 00:00:00
abstract：:Accumulation of the cytotoxic 40- to 42-residue beta-amyloid peptide represents the primary pathological process in Alzheimer's disease (AD). BACE1 (beta-site APP cleaving enzyme 1) is responsible for the initial required step in the neuronal amyloidogenic processing of beta-amyloid precursor protein and is a major dr...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi051040o
更新日期：2005-12-20 00:00:00
abstract：:The first committed step of guanine nucleotide biosynthesis is the oxidation of inosine 5'-monophosphate (IMP) to xanthosine 5'-monophosphate (XMP) catalyzed by IMP dehydrogenase. The reaction involves the reduction of NAD(+) with the formation of a covalent enzyme intermediate (E-XMP). Hydrolysis of E-XMP requires th...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi048342v
更新日期：2005-09-06 00:00:00
abstract：:Pulmonary surfactant protein D (SP-D), a member of the collectin group of innate immune proteins, plays important roles in lipopolysaccharide (LPS) recognition. We have previously shown that surfactant protein A (SP-A), a homologous collectin, interacts with Toll-like receptor (TLR) 2, resulting in alteration of TLR2-...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi060176z
更新日期：2006-07-18 00:00:00
abstract：:The photoreceptor protein rhodopsin has been reconstituted with a single phospholipid species, dimyristoylphosphatidylcholine, at a range of different lipid/protein ratios, and the exchange rate at the lipid-protein interface has been determined from the electron spin resonance spectra of spin-labeled phosphatidylchol...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00385a045
更新日期：1987-06-02 00:00:00
abstract：:The circular dichroism (CD) of Pfl filamentous virus has been examined over the temperature range 0-40 degrees C, in the absence and presence of Hg(II), Ag(I), and Cu(II). Thermal difference CD spectra were obtained by subtraction of spectra recorded above and below a thermally induced structure transition near 12 deg...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00410a010
更新日期：1988-05-17 00:00:00
abstract：:To contribute to the understanding of membrane protein function upon application of pressure as relevant for understanding, for example, the physiology of deep sea organisms or for baroenzymological biotechnical processes, we investigated the influence of hydrostatic pressure on the activity of Na+,K+-ATPase enriched ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi062235e
更新日期：2007-02-13 00:00:00
abstract：:We have isolated from rabbit liver three cDNA clones of 1400-1800 base pairs that hybridize selectively to RNA from animals treated with phenobarbital. The nucleotide sequences of the cDNAs have been determined. In the protein coding region the nucleotide sequences of two of the cDNAs are 88% homologous, and the third...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00297a005
更新日期：1984-01-17 00:00:00
abstract：:Ligand-dependent site-site (or subunit-subunit) interactions provide the basis for explaining cooperativity in chemical reactions. Even in the simplest possible nonaggregating system, interpretation of the interactions in terms of structural details requires an explicit assumption (or model) for the binding of the lig...
journal_title：Biochemistry
pub_type： 杂志文章,评审
doi：10.1021/bi00106a004
更新日期：1991-10-22 00:00:00
abstract：:N-RAP is a recently discovered muscle-specific protein found at cardiac intercalated disks. Double immunogold labeling of mouse cardiac muscle reveals that vinculin is located immediately adjacent to the fascia adherens region of the intercalated disk membrane, while N-RAP extends approximately 100 nm further toward t...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0107445
更新日期：2001-12-11 00:00:00
abstract：:Tumor suppressor Nore1, its acronym coming from novel Ras effector, is one of the 10 members of the Rassf (Ras association domain family) protein family that have been identified. It is expressed as two mRNA splice variants, Nore1A and a shorter isoform, Nore1B. It forms homo- and heterocomplexes through its C-termina...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi3014642
更新日期：2013-02-12 00:00:00
