Abstract：
:Acetylcholine receptors have been purified from junctional regions of normal rat diaphragm muscle and from extrajunctional regions of denervated diaphragm. The reaction of purified receptors with [122I]-alpha-bungarotoxin has been investigated by kinetic methods. The toxin-receptor complexes dissociated in a biphasic manner at 35 degrees with a rapidly dissociating component (t1/2 = 4 hr) and a slowly dissociating component (t1/2 is greater than or equal to 100 hr). The association reaction between toxin and receptor did not obey simple second-order kinetics but could be analyzed in terms of two classes of binding sites corresponding to the two rates of dissociation. This treatment of the data allowed derivation of association rate constants for the two sites. Value obtained for the dissociation constants were 3.7 times 10(-10) and less than or equal to 0.4 times 10(-10) M for the junctional receptor and 1.7 times 10(-10) and is less than or equal to 0.2 times 10(-10) M for the extrajunctional receptor. In each case it is the more tightly binding component that associates and dissociates more slowly. Receptors present in crude preparations were comparable to purified receptors in their reaction with [125I-alpha-bungarotoxin. The validity of the two site model is discussed in relation to the kinetic studies.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Brockes JP,Hall ZWdoi
10.1021/bi00681a008subject
Has Abstractpub_date
1975-05-20 00:00:00pages
2092-9issue
10eissn
0006-2960issn
1520-4995journal_volume
14pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract：:The sensitivity of the fluorescence energy of 2-p-toluidinylnaphthalene-6-sulfonate (TNS) to apparent solvent polarity is considerably greater in methanol-water mixtures and lower primary alcohols than in higher primary alcohols or solvents containing no hydroxyl groups. It is suggested that the behavior of TNS in low...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00675a016
更新日期：1975-02-25 00:00:00
abstract：:Upon cleavage of the reactive thioester bonds (Cys-949-Glx-952) of tetrameric human alpha 2-macroglobulin (alpha 2M) by methylamine, one sulfhydryl group per alpha 2M subunit is exposed. These identical sulfhydryl group sites were labeled with the thiol-specific nitroxide spin-labels (1-oxy-2,2,5,5-tetramethyl-3-pyrro...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00414a053
更新日期：1988-07-12 00:00:00
abstract：:It is known that, while melittin at micromolar concentrations is unfolded under conditions of low ionic strength at neutral pH, it adopts a tetrameric alpha-helical structure under conditions of high ionic strength, at alkaline pH, or at high peptide concentrations. To understand the mechanism of the conformational tr...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00118a014
更新日期：1992-01-28 00:00:00
abstract：:The "stalk" of the prokaryotic 50S ribosomal subunit is comprised of four copies of the protein L7/L12. In Escherichia coli, L7/L12 is a dimeric protein at micromolar concentrations, which is able to undergo rapid subunit exchange. A recent structural study indicated a tetrameric arrangement of the L12 proteins isolat...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi048015n
更新日期：2005-03-08 00:00:00
abstract：:The secretory component (SC) isolated from human milk was labeled with 2 mol of the fluorescent thiol reagent N-[7-(dimethylamino)-4-methylcoumarinyl]maleimide (DACM) per mol of SC through the reactive disulfide bond of SC. The binding of the labeled SC to polymeric immunoglobulins was examined by gel filtration by me...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00321a070
更新日期：1984-12-18 00:00:00
abstract：:The structures of two RNA duplexes, whose sequences correspond to portions of the ColE1 plasmid copy control RNA I and RNA II, have been determined. Crystals containing the 18mers 5'-CA CCGUUGGUAGCGGUGC-3' and 5'-CACCGCUACCAACGGUGC-3' diffract to 1.20 A resolution while those containing the 19mers 5'-GCACCGUUGGUAGCGGU...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi9912793
更新日期：1999-11-09 00:00:00
abstract：:In this study, we have mapped the intracellular alkylation sites of adozelesin and bizelesin, two potent analogs of CC-1065, in individual genes at the single-nucleotide level. Human colon carcinoma cells were treated with adozelesin and bizelesin, and the position of adducts were mapped within the PGK-1 and p53 genes...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00185a043
更新日期：1994-05-17 00:00:00
abstract：:Microtubules, ordinarily cold-labile structures, are made entirely resistant to cold temperature by the presence of substoichiometric amounts of STOP (stable tubule only polypeptide), a microtubule-associated protein. We have produced a monoclonal antibody which specifically recognizes a 145-kDa protein previously imp...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00428a064
更新日期：1989-01-24 00:00:00
abstract：:We have investigated the effect of increasing percentages of methylated pyrimidines on the structure of poly(dA-dU).poly(dA-dU). This was done by synthesizing analogous polynucleotides that contained deoxythymidine residues as well as deoxyuridine residues and observing their 31P NMR spectra in increasing amounts of C...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00341a033
更新日期：1985-09-24 00:00:00
abstract：:The surface immobilization methods that allowed single-molecule motility experiments with native kinesin have not worked with the ncd motor protein and other kinesin-related motors. To solve this problem, a surfactant (Pluronic F108) was chemically modified with the metal-chelating group nitrilotriacetic acid (NTA) to...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi9829175
更新日期：1999-04-20 00:00:00
abstract：:Prokaryotic promoters have been extensively studied to relate sequence features to promoter function. Here we examine the relationship between double-helix stability and promoter activity. The double-helix stability is evaluated from sequence data by free energy computation, based on reported values of dinucleotide fr...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00414a035
更新日期：1988-07-12 00:00:00
abstract：:Although plasma membrane (PM) cholesterol-rich and -poor domains have been isolated by subcellular fractionation, the real-time arrangement of cholesterol in such domains in living cells is still unclear. Therefore, dehydroergosterol (DHE), a naturally occurring fluorescent sterol, was incorporated into cultured L-cel...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi048172m
更新日期：2005-03-01 00:00:00
abstract：:The effects of metal ion binding on the optical spectroscopic properties and temperature stability of two single tryptophan mutants of chicken skeletal TnC, F78W and F154W, have been examined. The absence of tyrosine and other tryptophan residues allowed the unambiguous assignment of the spectral signal from the intro...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi982048j
更新日期：1999-09-14 00:00:00
abstract：:Bacillus subtilis CsoR (Bsu CsoR) is a copper-sensing transcriptional repressor that regulates the expression of the copZA operon encoding a copper chaperone and a Cu efflux P-type ATPase, respectively. Bsu CsoR is a homologue of Mycobacterium tuberculosis CsoR (Mtb CsoR), representative of a large Cu(I)-sensing regul...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi900115w
更新日期：2009-04-21 00:00:00
abstract：:Intrinsically disordered proteins (IDPs) are functionally active despite lacking a well-defined three-dimensional structure. Such proteins often undergo a disorder-to-order transition, or induced folding, when binding to their specific physiological partner. Because of cooperativity, the folding and binding steps typi...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.7b00350
更新日期：2017-07-25 00:00:00
abstract：:N1-(5'-Phosphoribosyl)adenosine-5'-monophosphate cyclohydrolase (HisI, PR-AMP cyclohydrolase) is a central enzyme in histidine biosynthesis catalyzing the hydrolysis of the N1-C6 bond of the purine substrate, a reaction unique to this pathway. A source of the recombinant monofunctional Methanococcus vannielii PR-AMP c...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi982475x
更新日期：1999-02-02 00:00:00
abstract：:The ATP analogue 5'-[p-(fluorosulfonyl)benzoyl]adenosine (FSBA) was used to chemically modify the ATP binding sites of the carbamyl phosphate synthetase domain of CAD, the multifunctional protein that catalyzes the first steps in mammalian pyrimidine biosynthesis. Reaction of CAD with FSBA resulted in the inactivation...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00106a033
更新日期：1991-10-22 00:00:00
abstract：:Inducible dimers are powerful tools for controlling biological processes through colocalizing signaling molecules. To be effective, an inducible system should have a dissociation constant in the "off" state that is greater (i.e., weaker affinity) than the concentrations of the molecules that are being controlled, and ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.6b00529
更新日期：2016-09-20 00:00:00
abstract：:Two glutathione S-transferase (GST) activities have been identified and purified from etiolated corn tissue. The first, designated GST I enzyme, is constitutively present in corn tissue, and the second, designated GST II enzyme, is present only in tissue which has been treated with chemical antidotes which protect cor...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00274a011
更新日期：1983-03-01 00:00:00
abstract：:Light-induced activation of the LOV2-Jα domain of the photoreceptor phototropin from oat is believed to involve the detachment of the Jα helix from the central β-sheet and its subsequent unfolding. The dynamics of these conformational changes were monitored by time-resolved emission spectroscopy with 100 ns time resol...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi101413v
更新日期：2010-12-28 00:00:00
abstract：:The activity of phospholipase A2 from snake venom to hydrolyze bilayers of phosphatidylcholines is greatly enhanced by the presence of the hydrolysis products, lysolecithin and fatty acid, in the bilayer. The fluorescence of several probes of membrane structure was used to monitor changes in bilayer physical propertie...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00024a003
更新日期：1995-06-20 00:00:00
abstract：:Previous work has described the novel ability to modulate in vitro the activity of restriction endonuclease NaeI from Nocardia aerocoligenes by using cleavable DNA and spermidine [Conrad & Topal (1989) Proc. Natl. Acad. Sci. U.S.A. 86, 9707-9711]. In this paper we report the results of a study of 49 type II restrictio...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00223a035
更新日期：1991-03-05 00:00:00
abstract：:A computational method has been developed to predict inhibitor binding energy for untested inhibitor molecules. A neural network is trained from the electrostatic potential surfaces of known inhibitors and their binding energies. The algorithm is then able to predict, with high accuracy, the binding energy of unknown ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi990830t
更新日期：1999-12-07 00:00:00
abstract：:Assignments have been made for 11 methyl, one Gln-C gamma, one Thr-C beta, and all six Tyr-C zeta carbon resonances of ribonuclease A. These partially serve to delineate the binding sites for Cu2+, Mn2+, phosphate, cytidine and its 2'-, 3'-, and 5'-phosphates (Cyd and Cyd-2'-P, -3'-P, and -5'-P), and one or a few urea...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00310a020
更新日期：1984-07-17 00:00:00
abstract：:The water-oxidizing complex of photosystem II cycles through five oxidation states, denoted S(i)() (i = 0-4), during water oxidation to molecular oxygen, which appears at the (transient) S(4) state. The recent detection of bimodal EPR signals from the S(3) state [Matsukawa, T., Mino, H., Yoneda, D., Kawamori, A. (1999...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0159938
更新日期：2002-07-30 00:00:00
abstract：:Synaptotagmin I is the calcium sensor in synchronous neurotransmitter release caused by fusion of synaptic vesicles with the presynaptic membrane in neurons. Synaptotagmin I interacts with acidic phospholipids, but also with soluble N-ethylmaleimide-sensitive factor attachment receptors (SNAREs), at various stages in ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi101864k
更新日期：2011-04-05 00:00:00
abstract：:The effects of phosphorylation at Ser40 of rat tyrosine hydroxylase on the affinities of catechols have been determined with both the ferric and ferrous forms of the enzyme. Phosphorylation had no effect on the Ki value for the inhibition of the ferrous enzyme by either dopamine or DOPA when the initial rate of turnov...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi980582l
更新日期：1998-06-23 00:00:00
abstract：:5-Iodonaphthyl 1-azide (INA) has been previously shown to selectively label, on photolysis, only those proteins in contact with the membrane lipids. Low concentrations (less than 10 microM) of INA added to rat ovarian plasma membranes induced, on photoactivation, a selective and complete loss of the response of the ad...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00298a016
更新日期：1984-01-31 00:00:00
abstract：:Mitochondria isolated from coenzyme Q deficient yeast cells had no detectable NADH:cytochrome c reductase or succinate:cytochrome c reductase activity but contained normal amounts of cytochromes b and c1 by spectral analysis. Addition of the exogenous coenzyme Q derivatives including Q2, Q6, and the decyl analogue (DB...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00354a031
更新日期：1986-03-25 00:00:00
abstract：:Signal transducer and activator of transcription factors (STATs) are proteins that can translocate into the nucleus, bind DNA, and activate gene transcription. STAT proteins play a crucial role in cell proliferation, apoptosis, and differentiation. The prevalent view is that STAT proteins are able to form dimers and b...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi501529x
更新日期：2015-09-08 00:00:00
