N1-(5'-phosphoribosyl)adenosine-5'-monophosphate cyclohydrolase: purification and characterization of a unique metalloenzyme.

abstract：:Histone deacetylases have proven to be attractive novel targets for the treatment of cancer. The first inhibitor of histone deacetylases was approved for the treatment of cutaneous T-cell lymphoma in 2006. The identification of new lead structures with improved effectiveness and fewer side effects is necessary. This r...

journal_title：Biochemistry

authors： Sykora J,Meyer-Almes FJ

更新日期：2010-02-23 00:00:00

abstract：:Lipoprotein(a) [Lp(a)] is a unique lipoprotein consisting of a low-density lipoprotein moiety (LDL) covalently linked to apoprotein(a). Previous work has demonstrated that Lp(a) can compete with plasminogen (PGN) for binding to endothelial and mononuclear cells and can inhibit PGN activation in cell-free systems. We h...

journal_title：Biochemistry

authors： Ezratty A,Simon DI,Loscalzo J

更新日期：1993-05-04 00:00:00

abstract：:In a previous paper, we reported the presence of globoside as a major neutral glycolipid in PC12 pheochromocytoma cells [Ariga, T., Macala, L. J., Saito, M., Margolis, R. K., Greene, L. A., Margolis, R. U., & Yu, R. K. (1988) Biochemistry 27, 52-58]. Recently, we found that subcloned PC12h cells accumulated another un...

journal_title：Biochemistry

authors： Ariga T,Yu RK,Scarsdale JN,Suzuki M,Kuroda Y,Kitagawa H,Miyatake T

更新日期：1988-07-12 00:00:00

abstract：:The MexA-MexB-OprM efflux pump exports structurally and functionally diverse xenobiotics and confers multi-drug resistance on Pseudomonas aeruginosa cells. The MexB transporter traverses the inner membrane twelve times, bears two large periplasmic domains and has two homologous tandem repeats. To test whether two homo...

journal_title：Biochemistry

authors： Eda S,Yoneyama H,Nakae T

更新日期：2003-06-17 00:00:00

abstract：:We describe a reconstitution of light-activated vertebrate photoreceptor GTPase and a purification of the GTP-binding protein (G protein), which is a component of the GTPase and modulates the light-activated phosphodiesterase (PDE) enzyme system. Rod outer segments (ROS) of bull frogs were treated with ethylenediamine...

journal_title：Biochemistry

authors： Shinozawa T,Bitensky MW

更新日期：1981-12-08 00:00:00

abstract：:We have recently reported that the Na pump alpha-subunit has cytosolic-oriented oligosaccharides which were sensitive to cleavage by an enzyme specific for hydrolysis of N-linked glycans [Pedemonte et al. (1990) Proc. Natl. Acad. Sci. U.S.A. 87, 9789-9793]. We now describe experiments that characterize the saccharides...

journal_title：Biochemistry

authors： Pedemonte CH,Kaplan JH

更新日期：1992-11-03 00:00:00

abstract：:The rubredoxin from Clostridium pasteurianum contains a single iron atom bound to the polypeptide chain by cysteines 6, 9, 39, and 42. The C42A variant of this protein has been prepared by site-directed mutagenesis and heterologous expression of the gene in Escherichia coli. The mutated protein was found to contain an...

journal_title：Biochemistry

authors： Meyer J,Gagnon J,Gaillard J,Lutz M,Achim C,Münck E,Pétillot Y,Colangelo CM,Scott RA

更新日期：1997-10-28 00:00:00

abstract：:A fluorescence quenching method was developed for determining partition coefficients and diffusional rates of small molecules in cell membranes. This method involves quenching the fluorescence of carbazole-labeled membranes by hydrophobic molecules that partition into membranes. Cell membrane phospholipids of mouse LM...

journal_title：Biochemistry

authors： Omann GM,Glaser M

更新日期：1984-10-09 00:00:00

abstract：:The binding to rat brain synaptosomes of a beta-scorpion toxin, i.e., toxin II of Centruroides suffusus suffusus (Css II), was studied as a function of pH, temperature, and concentration of some monovalent and divalent cations. At 10 degrees C and pH 6.0, the specific binding of 125I-labeled Css II corresponds to a si...

journal_title：Biochemistry

authors： Jover E,Bablito J,Couraud F

更新日期：1984-03-13 00:00:00

abstract：:Benzo[c]phenanthrene diol epoxide can covalently bind to the exocyclic amino group of deoxyadenosine to generate [BPh]dA adducts where the polycyclic aromatic hydrocarbon is attached to the major groove edge of DNA. This paper reports on NMR-energy minimization structural studies of the (+)-trans-anti-[BPh]dA adduct p...

journal_title：Biochemistry

authors： Cosman M,Fiala R,Hingerty BE,Laryea A,Lee H,Harvey RG,Amin S,Geacintov NE,Broyde S,Patel D

更新日期：1993-11-23 00:00:00

abstract：:The mechanism by which the heme-containing peroxidase, chloroperoxidase, is able to chlorinate substrates is poorly understood. One approach to advance our understanding of the mechanism of the enzyme is to determine those factors which contribute to its stability. In particular, under alkaline conditions, chloroperox...

journal_title：Biochemistry

authors： Blanke SR,Martinis SA,Sligar SG,Hager LP,Rux JJ,Dawson JH

更新日期：1996-11-19 00:00:00

abstract：:The reduction of D-amino acid oxidase (DAAO) by hydrated electrons (eaq-) has been studied in the absence and presence of benzoate by pulse radiolysis. The eaq-did not reduce the flavin moiety in DAAO and reacted with the amino acid residues in the protein. In the presence of benzoate, eaq- first reacted with benzoate...

journal_title：Biochemistry

authors： Kobayashi K,Hirota K,Ohara H,Hayashi K,Miura R,Yamano T

更新日期：1983-04-26 00:00:00

abstract：:Synthetic O-glycopeptides containing one or two GalNAc residues attached to Ser or Thr were used as substrates to investigate the effect of peptide structure on the activity of crude preparations of UDP-Gal:GalNAc alpha-R beta 3-Gal-transferase from pig stomach and pig and rat colonic mucosa and of a partially purifie...

journal_title：Biochemistry

authors： Brockhausen I,Möller G,Merz G,Adermann K,Paulsen H

更新日期：1990-11-06 00:00:00

abstract：:Fusion of a prokaryotic promoter to a yeast tRNA gene provides a means for uncoupling analyses of mutations affecting splicing from requirements for transcription and other processing steps. For this purpose, a phage lambda promoter was fused to the Saccharomyces cerevisiae tRNATyr(SUP3a) coding sequence. This fusion ...

journal_title：Biochemistry

authors： Shapero MH,Greer CL

更新日期：1991-07-02 00:00:00

abstract：:N-Linked sugar chains were liberated by hydrazinolysis from porcine zona pellucida glycoproteins obtained from ovarian follicular oocytes. Neutral sugar chains were separated from acidic ones by paper electrophoresis and fractionated with a serial lectin column chromatography and Bio-Gel P-4 column chromatography. The...

journal_title：Biochemistry

authors： Mori E,Takasaki S,Hedrick JL,Wardrip NJ,Mori T,Kobata A

更新日期：1991-02-26 00:00:00

abstract：:The baker's yeast saccharopine dehydrogenase (EC 1.5.1.7) was inactivated by 2,3-butanedione following pseudo-first-order reaction kinetics. The pseudo-first-order rate constant for inactivation was linearly related to the butanedione concentration, and a value of 7.5 M-1 min-1 was obtained for the second-order rate c...

journal_title：Biochemistry

authors： Fujioka M,Takata Y

更新日期：1981-02-03 00:00:00

abstract：:Mitomycins make up a group of antitumor natural products that are biosynthesized from aminohydroxybenzoic acid (AHBA) and N-acetylglucosamine (GlcNAc). While the biosynthetic gene cluster was reported two decades ago, the mechanism by which the two building blocks, AHBA and GlcNAc, are coupled during biosynthesis rema...

journal_title：Biochemistry

authors： Nguyen HP,Yokoyama K

更新日期：2019-06-25 00:00:00

abstract：:The effect of both antimitotic drugs and nucleotide analogues on the magnesium-induced self-association of purified tubulin into 42S double rings has been examined by sedimentation velocity. In the absence of magnesium, all complexes sedimented as the 5.8S species. The binding of colchicine to tubulin led to a small b...

journal_title：Biochemistry

authors： Shearwin KE,Timasheff SN

更新日期：1992-09-01 00:00:00

abstract：:D-Xylose isomerase (XI) is a heat-stable homotetrameric enzyme used in industry for the production of high-fructose corn syrups by isomerization of D-glucose into D-fructose. To carry out biochemical and structural studies of this enzyme and of its engineered variants, a rapid and convenient method of purification of ...

journal_title：Biochemistry

authors： Mrabet NT

更新日期：1992-03-17 00:00:00

abstract：:Site-directed mutagenesis and high-resolution two-dimensional (2D) proton nuclear magnetic resonance (NMR) were used to probe the structural and functional roles of a highly conserved residue, Asp-49, in the interfacial catalysis by bovine pancreatic phospholipase A2 (PLA2, overexpressed in Escherichia coli). Accordin...

journal_title：Biochemistry

authors： Li Y,Yu BZ,Zhu H,Jain MK,Tsai MD

更新日期：1994-12-13 00:00:00

abstract：:The conformational states of N-acylalanine dithio esters, involving rotational isomers about the RC(=O)NH--CH(CH3) and NHCH(CH3)--C(=S) bonds, are defined and compared to those of N-acylglycine dithio esters. The structure of N-(p-nitrobenzoyl)-DL-alanine ethyl dithio ester has been determined by X-ray crystallographi...

journal_title：Biochemistry

authors： Lee H,Angus RH,Storer AC,Varughese KI,Carey PR

更新日期：1988-01-12 00:00:00

abstract：:The packing of lipids into different aggregates, such as spheres, rods, or bilayers, is dependent on the hydrophobic volume, the hydrocarbon-water interfacial area, and the hydrocarbon chain length of the participating molecules, according to the self-assembly theory [Israelachvili, J. N., Marcelja, S., & Horn, R. G. ...

journal_title：Biochemistry

authors： Wieslander A,Rilfors L,Lindblom G

更新日期：1986-11-18 00:00:00

abstract：:Twelve site-directed mutants of rat cytochrome P450 2B1 distributed over seven positions and four putative substrate recognition sites (SRS) were constructed and expressed in COS cells. Function was examined using androstenedione and testosterone as substrates. Substitutions at positions 303, 360, and 473 did not mark...

journal_title：Biochemistry

authors： He Y,Luo Z,Klekotka PA,Burnett VL,Halpert JR

更新日期：1994-04-12 00:00:00

abstract：:The reduction kinetics of cytochrome P-450 are known to be biphasic, with a rapid initial phase and a slower subsequent phase, both of which appear linear in semilogarithmic plots. The present report demonstrates that these biphasic reduction kinetics can be described in terms of a preequilibrium between high- and low...

journal_title：Biochemistry

authors： Backes WL,Sligar SG,Schenkman JB

更新日期：1982-03-16 00:00:00

abstract：:The general view on Rhizobium chitolipooligosaccharides (CLOS) is that they are made in very low levels as diffusible molecules and are primarily secreted by the bacteria into the extracellular milieu where they interact with the host. However, the structural and predicted physicochemical properties of these amphiphil...

journal_title：Biochemistry

authors： Orgambide GG,Lee J,Hollingsworth RI,Dazzo FB

更新日期：1995-03-21 00:00:00

abstract：:The key polyamine catabolizing enzyme spermidine-spermine N1-acetyltransferase (SSAT) is among the few genes known to be inducible by the natural polyamines. Certain polyamine analogs markedly exaggerate this response and thus provide useful tools for studying the underlying regulatory mechanisms. As shown here, the a...

journal_title：Biochemistry

authors： Fogel-Petrovic M,Vujcic S,Brown PJ,Haddox MK,Porter CW

更新日期：1996-11-12 00:00:00

abstract：:Splicing factor RBM10 and its close homologues RBM5 and RBM6 govern the splicing of oncogenes such as Fas, NUMB, and Bcl-X. The molecular architecture of these proteins includes zinc fingers (ZnFs) and RNA recognition motifs (RRMs). Three of these domains in RBM10 that constitute the RNA binding part of this splicing ...

journal_title：Biochemistry

authors： Serrano P,Hammond JA,Geralt M,Wüthrich K

更新日期：2018-03-13 00:00:00

abstract：:Kinetics of flash-induced electron transfer from the soluble cytochrome c2 to the primary donor (P) of the reaction center purified from the purple bacterium Rhodobacter sphaeroides R-26 were investigated by time-resolved absorption spectroscopy. Re-reduction of P+ induced by a laser pulse was measured at 1283 nm both...

journal_title：Biochemistry

authors： Venturoli G,Mallardi A,Mathis P

更新日期：1993-12-07 00:00:00

abstract：:Nephrocalcin (NC), a calcium-binding glycoprotein of 14,000 molecular weight as a monomer, is known to inhibit the growth of calcium oxalate monohydrate (COM) crystals in renal tubules. We have isolated NC from bovine kidney tissue and purified into four isoforms, fractions A-D. NC-A and NC-B strongly inhibit the grow...

journal_title：Biochemistry

authors： Mustafi D,Nakagawa Y

更新日期：1996-11-26 00:00:00

abstract：:Hydrophobic interactions are responsible for stabilizing leucine zippers in peptides containing heptad repeats. The effects of substituting leucine by phenylalanine and alanine by glycine on the self-assembly of coiled-coils were examined in minimalist antimicrobial peptides designed to form amphipathic alpha-helices....

journal_title：Biochemistry

authors： Javadpour MM,Barkley MD

更新日期：1997-08-05 00:00:00