Abstract:
:The packing of lipids into different aggregates, such as spheres, rods, or bilayers, is dependent on the hydrophobic volume, the hydrocarbon-water interfacial area, and the hydrocarbon chain length of the participating molecules, according to the self-assembly theory [Israelachvili, J. N., Marcelja, S., & Horn, R. G. (1980) Q. Rev. Biophys. 13, 121-200]. The origin of the participating molecules should be of no importance with respect to their abilities to affect the above-mentioned parameters. In this investigation, Acholeplasma laidlawii, with a defined acyl chain composition of the membrane lipids, has been grown in the presence of three different classes of foreign molecules, known to partition into model and biological membranes. This results in an extensive metabolic alteration in the lipid polar head group composition, which is expressed as changes in the molar ratio between the lipids monoglucosyldiglyceride (MGDG) and diglucosyldiglyceride (DGDG), forming reversed hexagonal and lamellar phases in excess water, respectively. The formation of nonlamellar phases by A. laidlawii lipids depends critically upon the MGDG concentration [Lindblom, G., Brentel, I., Sjölund, M., Wikander, G., & Wieslander, A. (1986) Biochemistry (preceding paper in this issue)]. The foreign molecules tested belong to the following groups: nonpolar organic solvents, alcohols, and detergents. Their effects on the gel to liquid crystalline phase transition temperature (Tm), on the order parameter of the acyl chains, and on the phase equilibria between lamellar and nonlamellar liquid crystalline phases in lipid-water model systems are known in several instances.(ABSTRACT TRUNCATED AT 250 WORDS)
journal_name
Biochemistryjournal_title
Biochemistryauthors
Wieslander A,Rilfors L,Lindblom Gdoi
10.1021/bi00371a038subject
Has Abstractpub_date
1986-11-18 00:00:00pages
7511-7issue
23eissn
0006-2960issn
1520-4995journal_volume
25pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract::We have determined the formal potential values for each electron transfer to electron transfer flavoprotein-ubiquinone oxidoreductase (ETF-QO), in order to further characterize the thermodynamics of electron transport from various acyl-CoA thioesters to the mitochondrial ubiquinone pool. ETF-QO contains one [4Fe-4S]2+...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00162a012
更新日期:1992-12-01 00:00:00
abstract::The interaction of the TAR RNA element of human immunodeficiency virus type 1 (HIV-1) with a 2'-O-methyl analogue of an RNA hairpin aptamer previously identified by in vitro selection [Ducongé, F., and Toulmé, J. J. (1999) RNA 5, 1605-1614] was characterized by UV-monitored thermal denaturation and surface plasmon res...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi025974d
更新日期:2002-10-08 00:00:00
abstract::Sucrose density gradient shift assays were used to study the interactions of human glucocorticoid receptors (GR) with small DNA fragments either containing or lacking glucocorticoid response element (GRE) DNA consensus sequences. When crude cytoplasmic extracts containing [3H]triamcinolone acetonide [( 3H]TA) labeled ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00431a003
更新日期:1989-03-07 00:00:00
abstract::We have mapped the interface between the two transmembrane alpha-helices in the membrane domain of the Escherichia coli enzyme leader peptidase by analyzing disulfides formed between pairs of cysteine residues introduced near their respective periplasmic ends. The interface is formed primarily from aliphatic amino aci...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00084a020
更新日期:1993-08-24 00:00:00
abstract::The relative quantitative importance of the factors that determine the fidelity of translation is largely unknown, which makes predicting the extent to which the degeneracy of the genetic code can be broken challenging. Our strategy of using orthogonal tRNA/aminoacyl tRNA synthetase pairs to precisely direct the incor...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.8b00177
更新日期:2018-05-15 00:00:00
abstract::It is well-known that caldesmon (CaD) is a substrate for casein kinase II (CKII), and the phosphorylation of CaD by CKII regulates the interaction of CaD with myosin. However, the functionally relevant CKII phosphorylation site(s) on CaD and the precise role of CaD phosphorylation by CKII in mediating CaD's function h...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0006767
更新日期:2000-09-12 00:00:00
abstract::Basic peptides from the carboxy terminus of the human immunodeficiency virus type 1 (HIV-1) Tat protein bind to the stem-loop region of TAR RNA, spanning a trinucleotide bulge, with high affinity and moderate specificity. Previous studies have demonstrated that TAR RNA contains a specific arginine binding pocket. A se...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00027a041
更新日期:1995-07-11 00:00:00
abstract::Aromatic amino acid aminotransferase (ArAT) from Escherichia coli was overexpressed in E. coli cells, purified, and characterized. The enzyme was similar to aspartate aminotransferase (AspAT) of E. coli in many aspects, such as gross protein structure and spectroscopic properties. The reactions of pyridoxal 5'-phospha...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00096a036
更新日期:1993-11-16 00:00:00
abstract::Furamidine and related diamidines represent a promising series of drugs active against widespread parasites, in particular the Pneumocystic carinii pathogen. In this series, the phenylfuranbenzimidazole diamidine derivative DB293 was recently identified as the first unfused aromatic dication capable of forming stacked...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0108453
更新日期:2001-08-21 00:00:00
abstract::The surface immobilization methods that allowed single-molecule motility experiments with native kinesin have not worked with the ncd motor protein and other kinesin-related motors. To solve this problem, a surfactant (Pluronic F108) was chemically modified with the metal-chelating group nitrilotriacetic acid (NTA) to...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9829175
更新日期:1999-04-20 00:00:00
abstract::A full characterization of the thermodynamic forces underlying ligand-associated conformational changes in proteins is essential for understanding and manipulating diverse biological processes, including transport, signaling, and enzymatic activity. Recent experiments on the maltose binding protein (MBP) have provided...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi201481a
更新日期:2011-12-06 00:00:00
abstract::The Sir2 enzyme family is responsible for a newly classified chemical reaction, NAD(+)-dependent protein deacetylation. New peptide substrates, the reaction mechanism, and the products of the acetyl transfer to NAD(+) are described for SIR2. The final products of SIR2 reactions are the deacetylated peptide and the 2' ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi011858j
更新日期:2001-12-25 00:00:00
abstract::The Na+-translocating NADH:ubiquinone oxidoreductase (Na+-NQR) from Vibrio harveyi was purified and studied by EPR and visible spectroscopy. Two EPR signals in the NADH-reduced enzyme were detected: one, a radical signal, and the other a line around g = 1.94, which is typical for a [2Fe-2S] cluster. An E(m) of -267 mV...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi002545b
更新日期:2001-06-19 00:00:00
abstract::The [2Fe-2S] cluster of the Rieske iron-sulfur protein is held between two loops of the protein that are connected by a disulfide bridge. We have replaced the two cysteines that form the disulfide bridge in the Rieske protein of Saccharomyces cerevisiae with tyrosine and leucine, and tyrosine and valine, to evaluate t...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi035344r
更新日期:2003-11-25 00:00:00
abstract::The allosteric interactions that regulate substrate channeling and catalysis in the tryptophan synthase bienzyme complex from Salmonella typhimurium are triggered by covalent reactions at the beta-site and binding of substrate/product to the alpha-site. The transmission of these allosteric signals between the alpha- a...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi034291a
更新日期:2003-07-01 00:00:00
abstract::Extensive molecular modeling with molecular dynamics simulations and van der Waals energy analyses were used to elucidate the striking finding that a mutagenic benzo[a]pyrene-derived DNA lesion, the base-displaced intercalated 10R-(+)-cis-anti-B[a]P-N(2)-dG (G*), manifests large differences in nucleotide excision repa...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi4009177
更新日期:2013-08-20 00:00:00
abstract::Analysis of [gamma-32P]ATP in vitro labeled nuclear RNA shows transfer of gamma-32P from ATP to form the 5'-terminal monophosphate for large RNA molecules. This finding is an actively transcribing nuclear system capable of guanylation and methylation reactions indicates that polynucleotide kinase activity in the eukar...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00638a016
更新日期:1977-09-20 00:00:00
abstract::In the putative secondary structure of the transposon Tn10-encoded metal-tetracycline/H+ antiporter [Yamaguchi, et al. (1992) J. Biol. Chem. 267, 7490-7498], Tyr50-XXX-Gln54 in transmembrane helix 2 and Gly80-XXX-Asp84 in helix 3 are thought to face each other in the N-terminal region. At the corresponding positions i...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00072a027
更新日期:1993-06-01 00:00:00
abstract::We describe a fluorescence assay that can be used to monitor helicase-catalyzed unwinding of duplex DNA continuously in real time. The assay is based on the observation that fluorescence resonance energy transfer (FRET) occurs between donor (fluorescein) and acceptor (hexachlorofluorescein) fluorophores that are in cl...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00251a044
更新日期:1994-11-29 00:00:00
abstract::Apolipoprotein E (apoE)-knockout mice develop severe atherosclerosis associated with high levels of very low-density lipoprotein (VLDL) and intermediate-density lipoprotein (IDL) in plasma. To investigate the atherogenic role of VLDL and IDL, the lipoprotein fraction containing both VLDL and IDL (apoEko-VLDL/IDL) was ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi980762v
更新日期:1998-09-29 00:00:00
abstract::The factors that govern the substrate reactivity and stereoselectivity of phosphotriesterase (PTE) toward organophosphotriesters containing various combinations of methyl, ethyl, isopropyl, and phenyl substituents at the phosphorus center were determined by systematic alterations in the dimensions of the active site. ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi001549d
更新日期:2001-02-06 00:00:00
abstract::Blastochloris tepida is a newly described thermophilic purple bacterium containing bacteriochlorophyll b. Using purified light-harvesting 1 reaction center (LH1-RC) core complexes from Blc. tepida, we compared the biochemical, spectroscopic, and thermal denaturation properties of these complexes with those of its meso...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.0c00192
更新日期:2020-06-30 00:00:00
abstract::Some ascidians sequester vanadium and other metal ions that are bound and transported in higher organisms by transferrin. The ascidian Ciona intestinalis has a monolobal transferrin (nicatransferrin) in its plasma. The binding of vanadium(V) to nicatransferrin was investigated by using isothermal titration calorimetry...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi901630j
更新日期:2009-12-15 00:00:00
abstract::Solid-state NMR spectroscopy was employed to study the molecular dynamics of the colicin Ia channel domain in the soluble and membrane-bound states. In the soluble state, the protein executes small-amplitude librations (with root-mean-square angular fluctuations of 0-10 degrees ) in the backbone and larger-amplitude m...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0027231
更新日期:2001-06-26 00:00:00
abstract::Thrombospondin-1 (TSP1) binding to calreticulin (CRT) on the cell surface signals focal adhesion disassembly, leading to the intermediate adhesive phenotype, cell migration, anoikis resistance, and collagen stimulation. Residues Lys 24 and 32 in TSP1 and amino acids 24-26 and 32-34 in CRT have been shown through bioch...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi101639y
更新日期:2011-02-01 00:00:00
abstract::Prion proteins (PrP) are the infectious agent in transmissible spongiform encephalopathies (i.e., mad cow disease). To be infectious, prion proteins must undergo a conformational change involving a decrease in α-helical content along with an increase in β-strand content. This conformational change was evaluated by mea...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi1010514
更新日期:2011-03-29 00:00:00
abstract::Discoidal high-density lipoprotein (HDL) particles are known to be fractionalized into several discrete populations in plasma and to differ in behavior according to size; however, their structural differences and the factors regulating their size are less understood. In this study, we prepared several reconstituted HD...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi900785x
更新日期:2009-08-18 00:00:00
abstract::Human alpha1 and alpha2 isoforms of Na,K-ATPase have been expressed with porcine 10*Histidine-tagged beta1 subunit in Pichia pastoris. Methanol-induced expression of alpha2 is optimal at 20 degrees C, whereas at 25 degrees C, which is optimal for expression of alpha1, alpha2 is not expressed. Detergent-soluble alpha2b...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi701812c
更新日期:2007-12-25 00:00:00
abstract::The Bacillus subtilis DNA-dependent RNA polymerase holoenzyme and core enzyme each contain approximately two atoms of zinc per molecule. When the dissociated subunits of the enzyme are passed through a blue dextran-Sepharose affinity column, only the beta subunit binds to the column. The total zinc content of the enzy...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00632a012
更新日期:1977-06-28 00:00:00
abstract::An allelic variant of the ouabain-insensitive rat kidney Na+,K(+)-ATPase alpha 1-isoform was identified by chance in a cDNA library. The variant differed from the wild-type rat kidney Na+,K(+)-ATPase by a single G to C base substitution in the cDNA, which on the amino acid level gave rise to a glutamine in place of th...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00211a048
更新日期:1993-12-07 00:00:00