Chemistry of gene silencing: the mechanism of NAD+-dependent deacetylation reactions.

abstract：:Sedimentation in a preparative ultracentrifuge was used to determine the affinity of heavy meromyosin, HMM, for regulated actin, F-actin plus troponin-tropomyosin, in the presence of MgATP at pH 7.0, 20 degrees C, and mu = 18 mM. HMM was prepared from vertebrate striated muscle myosin by a mild chymotryptic digestion....

journal_title：Biochemistry

authors： Wagner PD,Stone DB

更新日期：1983-03-15 00:00:00

abstract：:The alpha-helix formed by the amino acid residues 9-19 of the B-chain of insulin is involved in the stabilization of its three-dimensional structure. We have shown that modification at positions B9, B10, B12, and B16 results in analogues possessing biological activities ranging from ca. 0.2% to ca. 500% relative to th...

journal_title：Biochemistry

authors： Hu SQ,Burke GT,Schwartz GP,Ferderigos N,Ross JB,Katsoyannis PG

更新日期：1993-03-16 00:00:00

abstract：:The reduction potentials of the compound II/ferric and compound I/compound II couples have been studied, using potassium hexachloroiridate as a mediator titrant, by thin-layer spectroelectrochemistry. Compound I, which is 2 equiv more oxidized than the ferric (i.e., resting) form of the enzyme, was reversibly formed v...

journal_title：Biochemistry

authors： Farhangrazi ZS,Fossett ME,Powers LS,Ellis WR Jr

更新日期：1995-03-07 00:00:00

abstract：:Recent studies of DNA hairpin loops have shown considerable dependence of the stability on the sequence of the loop [Senior, M., Jones, R. A., & Breslauer, K. J. (1988a) Proc. Natl. Acad. Sci. U.S.A. 85, 6242-6246; Xodo, L. E., Manzini, G., Quadrifoglio, F., van der Marel, G., & van Boom, J. H. (1989) Biochimie 71, 79...

journal_title：Biochemistry

authors： Wang S,Booher MA,Kool ET

更新日期：1994-04-19 00:00:00

abstract：:The receptor for atrial natriuretic peptide (ANP) is a type-I transmembrane protein containing an extracellular ligand-binding domain, a single transmembrane sequence, an intracellular kinase-homologous domain, and a guanylate cyclase (GCase) domain. Binding of ANP to the extracellular domain causes activation of the ...

journal_title：Biochemistry

authors： Misono KS,Sivasubramanian N,Berkner K,Zhang X

更新日期：1999-01-12 00:00:00

abstract：:The isolated cytochrome (cyt) b(6)f complex from spinach is inhibited by Cu(2+) with a K(D) of about 1 microM at pH 7.6 in the presence of 1.6 microM decyl-plastoquinol (C(10)-PQH(2)) as a substrate. Inhibition was competitive with respect to C(10)-PQH(2) but noncompetitive with respect to horse heart cyt c or plastoc...

journal_title：Biochemistry

authors： Rao BK,Tyryshkin AM,Roberts AG,Bowman MK,Kramer DM

更新日期：2000-03-28 00:00:00

abstract：:The first structure of a glycerol kinase from a Gram-positive organism, Enterococcus casseliflavus, has been determined to 2.8 A resolution in the presence of glycerol and to 2.5 A resolution in the absence of substrate. The substrate-induced closure of 7 degrees is significantly smaller than that reported for hexokin...

journal_title：Biochemistry

authors： Yeh JI,Charrier V,Paulo J,Hou L,Darbon E,Claiborne A,Hol WG,Deutscher J

更新日期：2004-01-20 00:00:00

abstract：:Pig pancreatic alpha-amylase (PPA), an enzyme belonging to the alpha-amylase family, is involved in the degradation of starch. Like some other members of this family, PPA requires chloride to reach maximum activity levels. To further explain the mechanism of chloride activation, a crystal of wild-type PPA soaked with ...

journal_title：Biochemistry

authors： Qian M,Ajandouz el H,Payan F,Nahoum V

更新日期：2005-03-08 00:00:00

abstract：:Barnase, the ribonuclease from Bacillus amyloliquefaciens, has been cloned and expressed in Escherichia coli [Hartley, R. W. (1988) J. Mol. Biol. 202, 913-915], thus enabling the overproduction and site-directed mutagenesis of one of the smallest enzymes (Mr equals 12,382). As barnase is also composed of just a single...

journal_title：Biochemistry

authors： Mossakowska DE,Nyberg K,Fersht AR

更新日期：1989-05-02 00:00:00

abstract：:Peptides corresponding to portions of curaremimetic neurotoxin loop 2 and to a structurally similar segment of rabies virus glycoprotein were synthetically modified in order to gain information on structure-function relationships of neurotoxin loop 2 interactions with the acetylcholine receptor. Binding of synthetic p...

journal_title：Biochemistry

authors： Lentz TL

更新日期：1991-11-12 00:00:00

abstract：:The electrophoretic mobilities of 24 single-stranded DNA oligomers, each containing 26 nucleotide residues, have been measured in polyacrylamide gels and in free solution. The mobilities observed at 20 degrees C differed by approximately 20% in polyacrylamide gels and by approximately 10% in free solution, even though...

journal_title：Biochemistry

authors： Stellwagen E,Abdulla A,Dong Q,Stellwagen NC

更新日期：2007-09-25 00:00:00

abstract：:The chemical shifts of all the aromatic proton and anomeric proton resonances of NADP+, NADPH, and several structural analogues have been determined in their complexes with Lactobacillus casei dihydrofolate reductase by double-resonance (saturation transfer) experiments. The binding of NADP+ to the enzyme leads to lar...

journal_title：Biochemistry

authors： Hyde EI,Birdsall B,Roberts GC,Feeney J,Burgen AS

更新日期：1980-08-05 00:00:00

abstract：:The inactivation of excited rhodopsin in the presence of ATP, rhodopsin kinase, and/or arrestin has been studied from its effect on the two subsequent steps in the light-induced enzymatic cascade: metarhodopsin II catalyzed activation of G-protein and G-protein-dependent activation of cGMP phosphodiesterase. The inact...

journal_title：Biochemistry

authors： Bennett N,Sitaramayya A

更新日期：1988-03-08 00:00:00

abstract：:Whether hydrogen bonds between side chains are energetically significant in proteins and peptides has been controversial. A method is given here for measuring these interactions in peptide helices by comparing the helix contents of peptides with 1, 2, or 3 interactions. Results are given for the glutamine--aspartate (...

journal_title：Biochemistry

authors： Huyghues-Despointes BM,Klingler TM,Baldwin RL

更新日期：1995-10-17 00:00:00

abstract：:In an earlier study, β³-puromycin was used for the selection of modified ribosomes, which were utilized for the incorporation of five different β-amino acids into Escherichia coli dihydrofolate reductase (DHFR). The selected ribosomes were able to incorporate structurally disparate β-amino acids into DHFR, in spite of...

journal_title：Biochemistry

authors： Maini R,Chowdhury SR,Dedkova LM,Roy B,Daskalova SM,Paul R,Chen S,Hecht SM

更新日期：2015-06-16 00:00:00

abstract：:N-[[(Iodoacetyl)amino]ethyl]-5-naphthylamine-1-sulfonic acid (IAEDANS) is a fluorescent reagent which reacts covalently with the free thiol groups of proteins. When the reagent is reacted with the Escherichia coli ribosome under mild conditions, gel electrophoresis shows modification of predominantly two proteins, S18...

journal_title：Biochemistry

authors： Hanas JS,Simpson MV

更新日期：1985-12-03 00:00:00

abstract：:We report on the effect of the viscogenic agents glycerol and ficoll on the RNase T1 catalyzed turnover of GpA, GpC, GpU, and Torula yeast RNA. For wild-type enzyme, we find that the kcat/Km values for the transesterification of GpC and GpA as well as for the cleavage of RNA are inversely proportional to the relative ...

journal_title：Biochemistry

authors： Steyaert J,Wyns L,Stanssens P

更新日期：1991-09-03 00:00:00

abstract：:Internal loops in RNA are important for folding and function. Consecutive noncanonical pairs can form in internal loops having at least two nucleotides on each side. Thermodynamic and structural insights into such internal loops should improve approximations for their stabilities and predictions of secondary and three...

journal_title：Biochemistry

authors： Chen G,Turner DH

更新日期：2006-03-28 00:00:00

abstract：:Nuclear Overhauser effect (NOE) measurements are reported for the nonexchangeable base and ribose 1' proton resonances of CCGGAp, ACCGGp, and ACCGGUp. The results permit assignment of these resonances to particular nucleotides in the sequences. The NOE data rule out conformations containing syn glycosidic linkages and...

journal_title：Biochemistry

authors： Petersheim M,Turner DH

更新日期：1983-01-18 00:00:00

abstract：:Progressive cerebral amyloid beta-protein (A beta) deposition is believed to play a central role in the pathogenesis of Alzheimer's disease (AD). Elevated levels of A beta(42) peptide formation have been linked to early-onset familial AD-causing gene mutations in the amyloid beta-protein precursor (A beta PP) and the ...

journal_title：Biochemistry

authors： Shearman MS,Beher D,Clarke EE,Lewis HD,Harrison T,Hunt P,Nadin A,Smith AL,Stevenson G,Castro JL

更新日期：2000-08-01 00:00:00

abstract：:Cysteine desulfurases (CDs) are pyridoxal-5'-phosphate (PLP)-dependent enzymes that cleave sulfur from cysteine via an enzyme cysteinyl persulfide intermediate. In vitro studies of these enzymes have generally employed dithiothreitol as a cosubstrate to reductively cleave the persulfide intermediate, and it has been s...

journal_title：Biochemistry

authors： Behshad E,Parkin SE,Bollinger JM Jr

更新日期：2004-09-28 00:00:00

abstract：:Investigation of the charge distribution for all known members of the PP-fold family of peptides reveals a common pattern characterized by a cluster of negative charges in the beta-turn region and a cluster of positive charges in the receptor-binding region of the peptide. Detailed analysis of the electrostatic proper...

journal_title：Biochemistry

authors： Bjørnholm B,Jørgensen FS,Schwartz TW

更新日期：1993-03-30 00:00:00

abstract：:Helices IV and V in the lactose permease of Escherichia coli contain the major determinants for substrate binding [Glu126 (helix IV), Arg144 (helix V), and Cys148 (helix V)]. Structural and dynamic features of this region were studied by using site-directed sulfhydryl modification of 48 single-Cys replacement mutants ...

journal_title：Biochemistry

authors： Kwaw I,Zen KC,Hu Y,Kaback HR

更新日期：2001-09-04 00:00:00

abstract：:The endogenous phosphoserine residue in porcine pepsinogen has been titrated with use of phosphorus-31 nuclear magnetic resonance (31P NMR). It has an observed pKa2 of 6.7 and a narrow line width (congruent to 10 Hz). The phosphate can be readily removed by an acid phosphatase from potato; however, it is resistant to ...

journal_title：Biochemistry

authors： Williams SP,Bridger WA,James MN

更新日期：1986-10-21 00:00:00

abstract：:The interaction of the retroviral nucleocapsid protein (NC) with nucleic acids forms the basis of its varied roles in the replication cycle, which include binding and condensing the viral RNA within the virion, stimulation of the early steps in reverse transcription, and dissociation from RNA in the replication comple...

journal_title：Biochemistry

authors： Secnik J,Gelfand CA,Jentoft JE

更新日期：1992-03-24 00:00:00

abstract：:A new specific voltage-sensitive calcium channel (VSCC) blocker has been isolated from the venom of the fish-hunting cone snail Conus consors. This peptide, named omega-Ctx CNVIIA, consists of 27 amino acid residues folded by 3 disulfide bridges. Interestingly, loop 4, which is supposed to be crucial for selectivity, ...

journal_title：Biochemistry

authors： Favreau P,Gilles N,Lamthanh H,Bournaud R,Shimahara T,Bouet F,Laboute P,Letourneux Y,Ménez A,Molgó J,Le Gall F

更新日期：2001-12-04 00:00:00

abstract：:The salivary antimicrobial peptide histatin-5 is able to aggregate and fuse negatively charged small unilamellar vesicles, and this fusogenic activity is selectively induced by the presence of zinc ions. Circular dichroism spectroscopy shows that histatin-5, in the presence of negatively charged vesicles and zinc ions...

journal_title：Biochemistry

authors： Melino S,Rufini S,Sette M,Morero R,Grottesi A,Paci M,Petruzzelli R

更新日期：1999-07-27 00:00:00

abstract：:Chemical modification of mitochondrial F1-ATPase from Schizosaccharomyces pombe by the tryptophan-specific reagent N-bromosuccinimide (NBS) at pH 5.0 in the presence of 20% glycerol produced a characteristic lowering in both enzyme absorbance at 280 nm and intrinsic fluorescence at 332 nm that varied with NBS/F1 molar...

journal_title：Biochemistry

authors： Divita G,Jault JM,Gautheron DC,Di Pietro A

更新日期：1993-02-02 00:00:00

abstract：:Single-molecule imaging of the entrance of a protein into the hydrophobic environment of a cell membrane was investigated. The pre-stem of LukF, one of the two components of the pore-forming toxin staphylococcal gamma-hemolysin, was specifically labeled with 6-bromoacetyl-2-dimethylaminonaphthalene (Badan), an environ...

journal_title：Biochemistry

authors： Nguyen AH,Nguyen VT,Kamio Y,Higuchi H

更新日期：2006-02-28 00:00:00

abstract：:Protein phosphatase 1 (PP1) is an essential and ubiquitous serine/threonine protein phosphatase that is regulated by more than 100 known inhibitor and targeting proteins. It is currently unclear how protein inhibitors distinctly and specifically regulate PP1 to enable rapid responses to cellular alterations. We demons...

journal_title：Biochemistry

authors： Dancheck B,Nairn AC,Peti W

更新日期：2008-11-25 00:00:00