Abstract：
:N-Linked sugar chains were liberated by hydrazinolysis from porcine zona pellucida glycoproteins obtained from ovarian follicular oocytes. Neutral sugar chains were separated from acidic ones by paper electrophoresis and fractionated with a serial lectin column chromatography and Bio-Gel P-4 column chromatography. Their structural analysis by sequential glycosidase digestion in combination with methylation analysis revealed that the neutral sugar chains are of bi-, tri-, and tetraantennary complex type with a fucosylated trimannosyl core. Twenty-six percent of the sugar chains contain N-acetyllactosamine repeating structures in their outer chain moieties. Only linear N-acetyllactosamine repeats, the maximum size of which is hexasaccharide, are detected. A characteristic feature is that 39% of the sugar chains contain N-acetylglucosamine residues at their nonreducing termini in spite of the absence of bisected sugar chains. This study provided, for the first time, the substantial information about the sugar chain structures of mammalian zona pellucida glycoproteins.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Mori E,Takasaki S,Hedrick JL,Wardrip NJ,Mori T,Kobata Adoi
10.1021/bi00222a012subject
Has Abstractpub_date
1991-02-26 00:00:00pages
2078-87issue
8eissn
0006-2960issn
1520-4995journal_volume
30pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract：:The structural, electronic, and catalytic properties of cytochrome P450cam are subtly altered when the cysteine that coordinates to the heme iron is replaced with a selenocysteine. To map the effects of the sulfur-to-selenium substitution on the individual steps of the catalytic cycle, we conducted a comparative kinet...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.5b00939
更新日期：2015-11-10 00:00:00
abstract：:The transfer of ganglioside GM1 from micelles to membranes and between different membrane populations has been examined by using a pyrene fatty acid derivative of the ganglioside. The transfer of gangliosides from micelles to membranes depends on the physical state as well as the molecular composition of the acceptor ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00375a032
更新日期：1987-01-13 00:00:00
abstract：:Previous investigations have shown that bovine growth hormone (bGH, somatotropin) unfolds through a reversible multistate process with at least one stable equilibrium intermediate. In extending our knowledge of the folding process for bGH, we demonstrate that a self-associated form of partially denatured bGH is formed...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00369a029
更新日期：1986-10-21 00:00:00
abstract：:It has been suggested that the mechanism of alkaline phosphatase (AP) is associative, or triester-like, because phosphorothioate monoesters are hydrolyzed by AP approximately 10(2)-fold slower than phosphate monoesters. This "thio effect" is similar to that observed for the nonenzymatic hydrolysis of phosphate trieste...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi000899x
更新日期：2000-08-08 00:00:00
abstract：:Studies utilizing the overexpression of individual isoforms indicated that both PKC-alpha and -delta promote a number of biological effects, including inhibition of DNA synthesis associated with rearrangements of the actin cytoskeleton in the murine B-cell lymphoma (Baf3), differentiation of the murine promyelocyte li...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi9731807
更新日期：1998-04-21 00:00:00
abstract：:The importance of the evolutionarily conserved Gly-4 residue for the affinity and kinetics of interaction of cystatin A with several cysteine proteinases was assessed by site-directed mutagenesis. Even the smallest replacement, by Ala, resulted in approximately 1000-, approximately 10- and approximately 6000-fold decr...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi980026r
更新日期：1998-05-19 00:00:00
abstract：:The generality of the concept of a rate-limiting step in enzymic reactions recently has been questioned [Northrop, D. B. (1981) Biochemistry 20, 4056-4061] because, in simulated isotopic experiments, alterations of the step identified as rate limiting by current definitions do not consistently affect Vmax in the expec...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00289a003
更新日期：1983-09-27 00:00:00
abstract：:The nature of the active site cofactor and the amino acid sequence flanking this structure have been determined in a range of copper amine oxidases. For enzymes from porcine plasma, porcine kidney, and pea seedlings, proteolytic digestion was performed on phenylhydrazone or p-nitrophenylhydrazone derivatives. Thermoly...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00163a025
更新日期：1992-12-08 00:00:00
abstract：:The unfolding equilibrium of recombinant (rNP) and natural variants of nucleoplasmin (NP) from Xenopus laevis has been analyzed using biochemical and spectroscopic techniques. In the presence of denaturing concentrations of guanidinium salts (GuHCl and GuSCN), both domains, core and tail, of the rNP pentamer unfold as...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi8002555
更新日期：2008-07-29 00:00:00
abstract：:The photochemical and subsequent thermal reactions of phoborhodopsin (pR490), which mediates the negative phototaxis (phobic reaction) of Halobacterium halobium, were investigated by low-temperature spectrophotometry. At room temperature, the absorption spectrum of pR490 displayed vibrational structure with a maximum ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00244a008
更新日期：1991-07-30 00:00:00
abstract：:The oxygen enzymatically inserted as a hydroxyl function by chick kidney homogenate into the 24 position of 25-hydroxyvitamin D3 to give 24,25-dihydroxyvitamin D3 is derived exclusively from 18O2. Therefore, like the 25-hydroxyvitamin D3-lalpha-hydroxylase system, the 25-hydroxyvitamin D3-24-hydroxylase system is also...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00629a015
更新日期：1977-05-17 00:00:00
abstract：:The structural and functional aspects along with equilibrium unfolding of procerain, a cysteine protease from Calotropis procera, were studied in solution. The energetic parameters and conformational stability of procerain in different states were also estimated and interpreted. Procerain belongs to the alpha + beta c...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi035047m
更新日期：2003-10-28 00:00:00
abstract：:In contrast to all other known pyrophosphatases, Moorella thermoacetica pyrophosphatase (mtCBS-PPase) contains nucleotide-binding CBS domains and is thus strongly regulated by adenine nucleotides. Stopped-flow measurements using a fluorescent AMP analogue, 2'(3')-O-(N-methylanthranoyl)-AMP (Mant-AMP), reveal that nucl...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi9019737
更新日期：2010-02-09 00:00:00
abstract：:The complete primary and three-dimensional solution structures of subtilosin A (1), a bacteriocin from Bacillus subtilis, were determined by multidimensional NMR studies on peptide produced using isotopically labeled [(13)C,(15)N]medium derived from Anabaena sp. grown on sodium [(13)C]bicarbonate and [(15)N]nitrate. A...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0359527
更新日期：2004-03-30 00:00:00
abstract：:The reduction kinetics of cytochrome P-450 are known to be biphasic, with a rapid initial phase and a slower subsequent phase, both of which appear linear in semilogarithmic plots. The present report demonstrates that these biphasic reduction kinetics can be described in terms of a preequilibrium between high- and low...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00535a034
更新日期：1982-03-16 00:00:00
abstract：:Human S100A7 (psoriasin) is a metal-chelating protein expressed by epithelial cells. It is a 22-kDa homodimer with two EF-hand domains per subunit and two transition-metal-binding His3Asp sites at the dimer interface. Each subunit contains two cysteine residues that can exist as free thiols (S100A7red) or as an intram...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.7b00781
更新日期：2017-10-31 00:00:00
abstract：:Mismatches in DNA occur naturally during replication and as a result of endogenous DNA damaging agents, but the mismatch repair (MMR) pathway acts to correct mismatches before subsequent rounds of replication. Rhodium metalloinsertors bind to DNA mismatches with high affinity and specificity and represent a promising ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi2015822
更新日期：2011-12-20 00:00:00
abstract：:The relative roles of insulin and glucose in the regulation of hepatic glycogen synthase and phosphorylase were studied in hepatocytes from fed rats. Elevation of extra-cellular glucose led to a rapid decrease in phosphorylase a activity followed by a slower increase in glycogen synthase I activity. A reciprocal and c...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00596a004
更新日期：1978-02-07 00:00:00
abstract：:The binding modes of four active site-directed, acylating inhibitors of human alpha-thrombin have been determined using X-ray crystallography. These inhibitors (GR157368, GR166081, GR167088, and GR179849) are representatives of a series utilizing a novel 5, 5-trans-lactone template to specifically acylate Ser195 of th...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi9830359
更新日期：1999-06-22 00:00:00
abstract：:Membrane-bound guanylate cyclases harbor a region called the dimerization or linker domain, which aids the enzymes in adopting an optimal monomer-monomer arrangement for catalysis. One subgroup of these guanylate cyclases is expressed in rod and cone cells of vertebrate retina, and mutations in the dimerization domain...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi400288p
更新日期：2013-07-30 00:00:00
abstract：:Class II tRNA synthetases have long been known to have quaternary structures of alpha, alpha2, alpha2beta2, and alpha4, depending on the amino acid specificity and the organism from which the synthetase was isolated. Even the quaternary structures of enzymes for the same amino acid show variations in evolution. The ba...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi971788+
更新日期：1997-12-09 00:00:00
abstract：:Nuclear Overhauser effect (NOE) measurements are reported for the nonexchangeable base and ribose 1' proton resonances of CCGGAp, ACCGGp, and ACCGGUp. The results permit assignment of these resonances to particular nucleotides in the sequences. The NOE data rule out conformations containing syn glycosidic linkages and...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00271a005
更新日期：1983-01-18 00:00:00
abstract：:Clostridium perfringens epsilon toxin belongs to the aerolysin-like family of pore-forming toxins and is one of the most potent bacterial toxins known. The epsilon toxin causes fatal enterotoxemia in sheep, goats, and possibly humans. Evidence indicates that the toxin binds to protein receptors including hepatitis A v...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi300690a
更新日期：2012-09-25 00:00:00
abstract：:This paper reports on a solution NMR characterization of the sequence selectivity and metal ion specificity in chromomycin-DNA oligomer complexes in the presence of divalent cations. The sequence selectivity studies have focused on chromomycin complexes with the self-complementary d(A1-A2-G3-G4-C5-C6-T7-T8) duplex con...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00501a012
更新日期：1990-12-11 00:00:00
abstract：:In this paper, we report kinetic studies for the chymotryptic activity of the 20S proteasome. Major observations include the following: (1) Reaction progress curves that are recorded at concentrations of Suc-Leu-Leu-Val-Tyr-AMC greater than about 40 microM are biphasic and characterized by initial velocities that deca...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi952262x
更新日期：1996-04-02 00:00:00
abstract：:The acetylenic analogue of adenosine 9-(5',6'-dideoxy-beta-D-ribo-hex-5'-ynofuranosyl)adenine has been synthesized, and its behavior as an inhibitor of bovine S-adenosylhomocysteine hydrolase has been examined. Incubation of the enzyme with excess inhibitor caused a time-dependent, irreversible inactivation of the enz...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00105a025
更新日期：1991-10-15 00:00:00
abstract：:Analogs of EPSP (4-8) have been prepared, and their activity as substrates for the chorismate synthase from Neurospora crassa has been characterized kinetically. The enzyme appears to show strict discrimination against substitution at the Z-position of the enol ether side chain as well as against substitution at the S...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00251a019
更新日期：1994-11-29 00:00:00
abstract：:The N-terminal region of alpha-spectrin is responsible for its association with beta-spectrin in a heterodimer, forming functional tetramers. Non-erythroid alpha-spectrin (alphaII-spectrin) has a significantly higher association affinity for beta-spectrin than the homologous erythroid alpha-spectrin (alphaI-spectrin)....
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi8013032
更新日期：2009-01-13 00:00:00
abstract：:The Salmonella typhimurium PhoN protein is a nonspecific acid phosphatase and belongs to the phosphatidic acid phosphatase type 2 (PAP2) superfamily. We report here the crystal structures of phosphate-bound PhoN, the PhoN-tungstate complex, and the T159D mutant of PhoN along with functional characterization of three m...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi062180g
更新日期：2007-02-27 00:00:00
abstract：:In type 2 diabetics, the hormone amylin misfolds into amyloid plaques implicated in the destruction of the pancreatic β-cells that make insulin and amylin. The aggregative misfolding of amylin is pH-dependent, and exposure of the hormone to acidic and basic environments could be physiologically important. Amylin has t...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi401164k
更新日期：2014-01-21 00:00:00
