Photoreaction cycle of phoborhodopsin studied by low-temperature spectrophotometry.

Abstract:

:The photochemical and subsequent thermal reactions of phoborhodopsin (pR490), which mediates the negative phototaxis (phobic reaction) of Halobacterium halobium, were investigated by low-temperature spectrophotometry. At room temperature, the absorption spectrum of pR490 displayed vibrational structure with a maximum at 490 nm and a shoulder at 460 nm, which were remarkably sharpened by cooling, resulting in the appearance of two well-separated peaks. On irradiation of pR490 at -170 degrees C, a photo-steady-state mixture composed of pR490 and two photoproducts, P520 and P480, was formed. P480 had an absorption maximum at 480 nm and thermally converted to pR490 above -160 degrees C, while P520 had an absorption maximum at 515 nm and thermally converted to P350, the next intermediate, above -60 degrees C. Above -30 degrees C, P350 was converted to P530, and then reverted to pR490. P520, P350, and P530 may correspond to K, M, and O intermediates of bacteriorhodopsin, respectively, on the basis of their absorption spectra, but the intermediates corresponding to L and N intermediates were not observed. On the basis of these results, a new scheme of the photoreaction cycle of pR490 was presented.

journal_name

Biochemistry

journal_title

Biochemistry

authors

Imamoto Y,Shichida Y,Yoshizawa T,Tomioka H,Takahashi T,Fujikawa K,Kamo N,Kobatake Y

doi

10.1021/bi00244a008

subject

Has Abstract

pub_date

1991-07-30 00:00:00

pages

7416-24

issue

30

eissn

0006-2960

issn

1520-4995

journal_volume

30

pub_type

杂志文章
  • Characterization of cDNA clones encoding rabbit and human serum paraoxonase: the mature protein retains its signal sequence.

    abstract::Serum paraoxonase hydrolyzes the toxic metabolites of a variety of organophosphorus insecticides. High serum paraoxonase levels appear to protect against the neurotoxic effects of organophosphorus substrates of this enzyme [Costa et al. (1990) Toxicol. Appl. Pharmacol. 103, 66-76]. The amino acid sequence accounting f...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00106a010

    authors: Hassett C,Richter RJ,Humbert R,Chapline C,Crabb JW,Omiecinski CJ,Furlong CE

    更新日期:1991-10-22 00:00:00

  • Basic and acidic regions flanking the HMG-box domain of maize HMGB1 and HMGB5 modulate the stimulatory effect on the DNA binding of transcription factor Dof2.

    abstract::The chromatin-associated high-mobility group (HMG) proteins of the plant HMGB family are characterized by a central HMG-box domain that is flanked by a basic N-terminal and an acidic C-terminal domain. By functional interaction with certain transcription factors, HMGB proteins contribute to transcriptional regulation....

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi6024947

    authors: Grasser M,Christensen JM,Peterhänsel C,Grasser KD

    更新日期:2007-05-29 00:00:00

  • Electronic structure of the metal center in the Cd(2+), Zn(2+), and Cu(2+) substituted forms of KDO8P synthase: implications for catalysis.

    abstract::Aquifex aeolicus 3-deoxy-d-manno-octulosonate 8-phosphate synthase (KDO8PS) is active with a variety of different divalent metal ions bound in the active site. The Cd(2+), Zn(2+), and Cu(2+) substituted enzymes display similar values of k(cat) and similar dependence of K(m)(PEP) and K(m)(A5P) on both substrate and pro...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi801955h

    authors: Kona F,Tao P,Martin P,Xu X,Gatti DL

    更新日期:2009-04-28 00:00:00

  • Molecular dynamics, free energy, and SPR analyses of the interactions between the SH2 domain of Grb2 and ErbB phosphotyrosyl peptides.

    abstract::We studied the interactions between the SH2 domain of growth factor receptor binding protein 2 (Grb2) and ErbB receptor-derived phosphotyrosyl peptides using molecular dynamics, free energy calculations, and surface plasmon resonance (SPR) analysis. Binding free energies for nine phosphotyrosyl peptides were calculate...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi034113h

    authors: Suenaga A,Hatakeyama M,Ichikawa M,Yu X,Futatsugi N,Narumi T,Fukui K,Terada T,Taiji M,Shirouzu M,Yokoyama S,Konagaya A

    更新日期:2003-05-13 00:00:00

  • Bacterial ApbC can bind and effectively transfer iron-sulfur clusters.

    abstract::The metabolism of iron-sulfur ([Fe-S]) clusters requires a complex set of machinery that is still being defined. Mutants of Salmonella enterica lacking apbC have nutritional and biochemical properties indicative of defects in [Fe-S] cluster metabolism. ApbC is a 40.8 kDa homodimeric ATPase and as purified contains lit...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi800551y

    authors: Boyd JM,Pierik AJ,Netz DJ,Lill R,Downs DM

    更新日期:2008-08-05 00:00:00

  • Efficient and selective photoaffinity labeling of the estrogen receptor using two nonsteroidal ligands that embody aryl azide or tetrafluoroaryl azide photoreactive functions.

    abstract::3-(4-Azido-2,3,5,6-tetrafluorobenzoyl)-6-hydroxy-2-(4- hydroxyphenyl)benzo[b]thiophene 1 (tetrafluoroaryl azide, TFAA) and its protio analogue 3-(4-azidobenzoyl)-6- hydroxy-2-(4-hydroxyphenyl)benzo[b]thiophene 2 (protioaryl azide, PAA), photoaffinity labeling (PAL) reagents for the estrogen receptor (ER), have been pr...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00223a018

    authors: Pinney KG,Carlson KE,Katzenellenbogen BS,Katzenellenbogen JA

    更新日期:1991-03-05 00:00:00

  • Regulatory mechanisms of tumor suppressor P16(INK4A) and their relevance to cancer.

    abstract::P16(INK4A) (also known as P16 and MTS1), a protein consisting exclusively of four ankyrin repeats, is recognized as a tumor suppressor mainly because of the prevalence of genetic inactivation of the p16(INK4A) (or CDKN2A) gene in virtually all types of human cancers. However, it has also been shown that an elevated le...

    journal_title:Biochemistry

    pub_type: 杂志文章,评审

    doi:10.1021/bi200642e

    authors: Li J,Poi MJ,Tsai MD

    更新日期:2011-06-28 00:00:00

  • Effects of ethylene glycol and methanol on ammonia-induced structural changes of the oxygen-evolving complex in photosystem II.

    abstract::Ammonia is an inhibitor of water oxidation and a structural analogue for substrate water, making it a valuable probe for the structural properties of the possible substrate-binding site on the oxygen-evolving complex (OEC) in photosystem II (PSII). By using the NH(3)-induced upshift of the 1365 cm(-)(1) IR mode in the...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi050030k

    authors: Fang CH,Chiang KA,Hung CH,Chang K,Ke SC,Chu HA

    更新日期:2005-07-19 00:00:00

  • FtsZ filament dynamics at steady state: subunit exchange with and without nucleotide hydrolysis.

    abstract::We have measured three aspects of FtsZ filament dynamics at steady state: rates of GTP hydrolysis, subunit exchange between protofilaments, and disassembly induced by dilution or excess GDP. All three reactions were slowed with an increase in the potassium concentration from 100 to 500 mM, via replacement of potassium...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi8022653

    authors: Chen Y,Erickson HP

    更新日期:2009-07-21 00:00:00

  • Differential cytotoxicity of human wild type and mutant alpha-synuclein in human neuroblastoma SH-SY5Y cells in the presence of dopamine.

    abstract::Parkinson's disease (PD) involves loss of dopaminergic neurons in the substantia nigra and is characterized by intracellular inclusions, Lewy bodies, consisting primarily of aggregated alpha-synuclein. Two substitution mutations (A53T and A30P) in alpha-synuclein gene have been identified in familial early-onset PD. T...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi036114f

    authors: Moussa CE,Wersinger C,Tomita Y,Sidhu A

    更新日期:2004-05-11 00:00:00

  • Characterization of a heparin-binding site on the catalytic domain of factor XIa: mechanism of heparin acceleration of factor XIa inhibition by the serpins antithrombin and C1-inhibitor.

    abstract::Heparin accelerates inhibition of factor XIa (fXIa) by the serpins antithrombin (AT) and C1-inhibitor (C1-INH) by more than 2 orders of magnitude. The mechanism of the heparin-mediated acceleration of fXIa inhibition by these serpins is incompletely understood, as heparin appears to interact with both the catalytic an...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi802298r

    authors: Yang L,Sun MF,Gailani D,Rezaie AR

    更新日期:2009-02-24 00:00:00

  • Phospholipids stabilize the interaction between the alpha and beta subunits of the solubilized receptor for immunoglobulin E.

    abstract::The cell-surface component (alpha) which binds monomeric immunoglobulin E with high affinity is associated with a second polypeptide (beta) in the plasma membrane. The latter component tends to dissociate during purification of the alpha chain from detergent extracts of cells, even at neutral pHs and physiological ion...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00269a047

    authors: Rivnay B,Wank SA,Poy G,Metzger H

    更新日期:1982-12-21 00:00:00

  • Conformational heterogeneity at position U37 of an all-RNA hairpin ribozyme with implications for metal binding and the catalytic structure of the S-turn.

    abstract::The hairpin ribozyme is an RNA enzyme that performs site-specific phosphodiester bond cleavage between nucleotides A-1 and G+1 within its cognate substrate. Previous functional studies revealed that the minimal hairpin ribozyme exhibited "gain-of-function" cleavage properties resulting from U39C or U39 to propyl linke...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi051550i

    authors: Alam S,Grum-Tokars V,Krucinska J,Kundracik ML,Wedekind JE

    更新日期:2005-11-08 00:00:00

  • Secretory carrier membrane protein SCAMP2 and phosphatidylinositol 4,5-bisphosphate interactions in the regulation of dense core vesicle exocytosis.

    abstract::Secretory carrier membrane protein 2 (SCAMP2) functions in late steps of membrane fusion in calcium-dependent granule exocytosis. A basic/hydrophobic peptide segment within SCAMP2 (SCAMP2 E: CWYRPIYKAFR) has been implicated in this function and shown to bind and sequester phosphatidylinositol 4,5-bisphosphate [PI(4,5)...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi701121j

    authors: Liao H,Ellena J,Liu L,Szabo G,Cafiso D,Castle D

    更新日期:2007-09-25 00:00:00

  • N-(5-Phosphoribosyl)anthranilate isomerase-indoleglycerol-phosphate synthase. 2. Fast-reaction studies show that a fluorescent substrate analogue binds independently to two different sites.

    abstract::The mechanism of binding of reduced 1-(2-carboxyphenylamino)-1-deoxyribulose 5-phosphate (rCdRP) to two different binding sites on the bifunctional enzyme is determined by kinetic studies, using temperature-jump and stopped-flow equipment with fluorescence detection. Two rapid binding processes and a comparatively slo...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00593a030

    authors: Cohn W,Kirschner K,Paul C

    更新日期:1979-12-25 00:00:00

  • Characterization and stereochemistry of cofactor oxidation by a type II dihydrofolate reductase.

    abstract::Type II dihydrofolate reductases (DHFRs) encoded by the R67 and R388 plasmids are different both in sequence and in structure from known chromosomal DHFRs. These plasmid-derived DHFRs are responsible for conferring trimethoprim resistance to the host strain. A derivative of R388 DHFR, RBG200, has been cloned and overp...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00494a011

    authors: Brito RM,Reddick R,Bennett GN,Rudolph FB,Rosevear PR

    更新日期:1990-10-23 00:00:00

  • Interaction of profilin with G-actin and poly(L-proline).

    abstract::The interaction of bovine spleen profilin with ATP- and ADP-G-actin and poly(L-proline) has been studied by spectrofluorimetry, analytical ultracentrifugation, and rapid kinetics in low ionic strength buffer. Profilin binding to G-actin is accompanied by a large quenching of tryptophan fluorescence, allowing the measu...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00194a011

    authors: Perelroizen I,Marchand JB,Blanchoin L,Didry D,Carlier MF

    更新日期:1994-07-19 00:00:00

  • Structure of the potassium form of CGCGAATTCGCG: DNA deformation by electrostatic collapse around inorganic cations.

    abstract::The potassium form of d(CGCGAATTCGCG) solved by X-ray diffraction to 1.75 A resolution indicates that monovalent cations penetrate the primary and secondary layers of the "spine of hydration". Both the sodium [Shui, X., McFail-Isom, L., Hu, G. G., and Williams, L. D. (1998) Biochemistry 37, 8341-8355] and the potassiu...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi982063o

    authors: Shui X,Sines CC,McFail-Isom L,VanDerveer D,Williams LD

    更新日期:1998-12-01 00:00:00

  • Large changes in the CRAC segment of gp41 of HIV do not destroy fusion activity if the segment interacts with cholesterol.

    abstract::The membrane-proximal external region (MPER) of the gp41 fusion protein of HIV is highly conserved among isolates of this virus and is considered a target for vaccine development. This region also appears to play a role in membrane fusion as well as localization of the virus to cholesterol-rich domains in membranes. T...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi8014828

    authors: Vishwanathan SA,Thomas A,Brasseur R,Epand RF,Hunter E,Epand RM

    更新日期:2008-11-11 00:00:00

  • Comparisons of interfacial Phe, Tyr, and Trp residues as determinants of orientation and dynamics for GWALP transmembrane peptides.

    abstract::Aromatic amino acids often flank the transmembrane alpha helices of integral membrane proteins. By favoring locations within the membrane-water interface of the lipid bilayer, aromatic residues Trp, Tyr, and sometimes Phe may serve as anchors to help stabilize a transmembrane orientation. In this work, we compare the ...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi500439x

    authors: Sparks KA,Gleason NJ,Gist R,Langston R,Greathouse DV,Koeppe RE 2nd

    更新日期:2014-06-10 00:00:00

  • Structure-sensitive RNA footprinting of yeast nuclear ribonuclease P.

    abstract::Several enzymatic and chemical reagents were used to probe the secondary structure of Saccharomyces cerevisiae nuclear RNase P RNA in the presence and absence of its protein components. Double-stranded regions were detected with RNase V1 and single-stranded regions with RNase ONE (Escherichia coli RNase I). Nucleotide...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00173a022

    authors: Tranguch AJ,Kindelberger DW,Rohlman CE,Lee JY,Engelke DR

    更新日期:1994-02-22 00:00:00

  • Expanding the Genetic Code of a Photoautotrophic Organism.

    abstract::The photoautotrophic freshwater cyanobacterium Synechococcus elongatus is widely used as a chassis for biotechnological applications as well as a photosynthetic bacterial model. In this study, a method for expanding the genetic code of this cyanobacterium has been established, thereby allowing the incorporation of unn...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/acs.biochem.7b00131

    authors: Chemla Y,Friedman M,Heltberg M,Bakhrat A,Nagar E,Schwarz R,Jensen MH,Alfonta L

    更新日期:2017-04-25 00:00:00

  • Analysis of the substrate specificity of the Staphylococcus aureus sortase transpeptidase SrtA.

    abstract::The Staphylococcus aureus sortase transpeptidase SrtA isoform is responsible for the covalent attachment of virulence and colonization-associated proteins to the bacterial peptidoglycan. SrtA utilizes two substrates, undecaprenol-pyrophosphoryl-MurNAc(GlcNAc)-Ala-D-isoGlu-Lys(epsilon-Gly(5))-D-Ala-D-Ala (branched Lipi...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi035920j

    authors: Kruger RG,Otvos B,Frankel BA,Bentley M,Dostal P,McCafferty DG

    更新日期:2004-02-17 00:00:00

  • Size of acetylcholine receptors in the membrane. An improved version of the radiation inactivation method.

    abstract::The radiation inactivation method was used to study the size of acetylcholine receptors in the intact membrane-bound state. This technique was reinvestigated, and modifications were made which remove substantial difficulties affecting previous applications of it to such proteins. The molecular size was deduced here by...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00538a033

    authors: Lo MM,Barnard EA,Dolly JO

    更新日期:1982-04-27 00:00:00

  • Protein influences on porphyrin structure in cytochrome c: evidence from Raman difference spectroscopy.

    abstract::To probe the details of protein heme interactions, we have developed a Raman difference spectroscopic technique, which allows reliable detection of very small, approximately equal to 0.01 cm-1, frequency differences. When this technique is applied to heme proteins, structural differences in the protein which perturb t...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00525a030

    authors: Shelnutt JA,Rousseau DL,Dethmers JK,Margoliash E

    更新日期:1981-10-27 00:00:00

  • Effects of chromogranin expression on inositol 1,4,5-trisphosphate-induced intracellular Ca2+ mobilization.

    abstract::We show here that expression of chromogranins in non-neuroendocrine NIH3T3 cells significantly increased the amount of IP(3)-mediated intracellular Ca(2+) mobilization in these cells, whereas suppression of them in neuroendocrine PC12 cells decreased the amount of mobilized Ca(2+). We have therefore investigated the r...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi048070w

    authors: Huh YH,Jeon SH,Yoo JA,Park SY,Yoo SH

    更新日期:2005-04-26 00:00:00

  • Picosecond time-resolved fluorescence studies on excitation energy transfer in a histidine 117 mutant of the D2 protein of photosystem II in Synechocystis 6803.

    abstract::The role of the peripheral reaction center chlorophyll a molecule associated with His117 of the D2 polypeptide in photosystem II was investigated in Synechocystis sp. PCC 6803 using a combination of steady state, pump-probe, and picosecond time-resolved fluorescence spectroscopy. Data were obtained from intact cells a...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi000476v

    authors: Vasil'ev S,Bruce D

    更新日期:2000-11-21 00:00:00

  • Phosphoinositide-specific phospholipase C-delta 1: effect of monolayer surface pressure and electrostatic surface potentials on activity.

    abstract::We added phospholipase C-delta 1 (PLC-delta) to the aqueous subphase beneath monolayers formed from mixtures of phosphatidylinositol 4,5-bisphosphate (2% PIP2), phosphatidylserine (33% PS), and phosphatidylcholine (65% PC) and then measured the initial rate of hydrolysis of PIP2 after addition of 10 microM free calciu...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00166a006

    authors: Rebecchi M,Boguslavsky V,Boguslavsky L,McLaughlin S

    更新日期:1992-12-29 00:00:00

  • Inhibition of HIV protease activity by heterodimer formation.

    abstract::The dimeric nature of the HIV protease has been exploited to devise a novel mode of inhibiting the enzyme. The use of defective monomers or nonidentical subunits to exchange with wild-type homodimers produces catalytically defective heterodimers. Incubation of the HIV1 or HIV2 protease with a 4-fold molar excess of an...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00215a016

    authors: Babé LM,Pichuantes S,Craik CS

    更新日期:1991-01-08 00:00:00

  • Kinetic and secondary structure analysis of Naegleria andersoni GIR1, a group I ribozyme whose putative biological function is site-specific hydrolysis.

    abstract::NanGIR1 is a catalytic element inserted in the P6 loop of a group I intron (NanGIR2) in the small subunit rRNA precursor of the protist Naegleria andersoni [Einvik, C., Decatur, W. A., Embley, T. M., Vogt, V. M., and Johansen, S. (1997) RNA 3, 710-720]. It catalyzes site-specific hydrolysis at an internal processing s...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi9718595

    authors: Jabri E,Aigner S,Cech TR

    更新日期:1997-12-23 00:00:00