Abstract：
:Membrane-bound guanylate cyclases harbor a region called the dimerization or linker domain, which aids the enzymes in adopting an optimal monomer-monomer arrangement for catalysis. One subgroup of these guanylate cyclases is expressed in rod and cone cells of vertebrate retina, and mutations in the dimerization domain of rod outer segment guanylate cyclase 1 (ROS-GC1, encoded by the GUCY2D gene) correlate with retinal cone-rod dystrophies. We investigate how a Q847L/K848Q double mutation, which was found in patients suffering from cone-rod dystrophy, and the Q847L and K848Q single-point mutations affect the regulatory mechanism of ROS-GC1. Both the wild type and mutants of heterologously expressed ROS-GC1 were present in membranes. However, the mutations affected the catalytic properties of ROS-GC1 in different manners. All mutants had higher basal guanylate cyclase activities but lower levels of activation by Ca²⁺-sensing guanylate cyclase-activating proteins (GCAPs). Further, incubation with wild-type GCAP1 and GCAP2 revealed for all ROS-GC1 mutants a shift in Ca²⁺ sensitivity, but activation of the K848Q mutant by GCAPs was severely impaired. Apparent affinities for GCAP1 and GCAP2 were different for the double mutant and the wild type. Circular dichroism spectra of the dimerization domain showed that the wild type and mutants adopt a prevalently α-helical structure, but mutants exhibited lower thermal stability. Our results indicate that the dimerization domain serves as a Ca²⁺-sensitive control module. Although it is per se not a Ca²⁺-sensing unit, it seems to integrate and process information regarding Ca²⁺ sensing by sensor proteins and regulator effector affinity.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Zägel P,Dell'Orco D,Koch KWdoi
10.1021/bi400288psubject
Has Abstractpub_date
2013-07-30 00:00:00pages
5065-74issue
30eissn
0006-2960issn
1520-4995journal_volume
52pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract：:Vibrational Raman spectroscopic experiments have been performed as a function of temperature on aqueous dispersions of synthetic DL-erythro-N-lignoceroylsphingosylphosphocholine [C(24):SPM], a racemic mixture of two highly asymmetric hydrocarbon chain length sphingomyelins. Raman spectral peak-height intensity ratios ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00343a036
更新日期：1985-10-22 00:00:00
abstract：:A detailed characterization of the main chain and side chain dynamics in R. capsulatus ferrocytochrome c(2) derived from (2)H NMR relaxation of methyl group resonances is presented. (15)N relaxation measurements confirm earlier results indicating that R. capsulatus ferrocytochrome c(2) exhibits minor rotational anisot...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0102252
更新日期：2001-06-05 00:00:00
abstract：:We report nuclear magnetic resonance (NMR) data indicating that the Rieske protein from the cytochrome bc complex of Thermus thermophilus (TtRp) undergoes modest redox-state-dependent and ligand-dependent conformational changes. To test models concerning the mechanism by which TtRp transfers between different sites on...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi400296c
更新日期：2013-04-30 00:00:00
abstract：:Excluded volume and viscosity effects of crowding agents that mimic crowded conditions in vivo on "classical" burst phase folding kinetics of cytochrome c are assessed in vitro. Upon electron transfer-triggered folding of reduced cytochrome c, far-UV time-resolved circular dichroism (TRCD) is used to monitor folding u...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi301324y
更新日期：2012-12-11 00:00:00
abstract：:The trafficking kinetics of GLUT4 and GLUT1 in rat epididymal adipocytes were analyzed by a four-compartment model based upon steady-state pool sizes of three intracellular fractions and one plasma membrane fraction separated and assessed under both basal and insulin-stimulated states. The steady-state compartment siz...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0007021
更新日期：2000-08-08 00:00:00
abstract：:The unique structural properties of the ferritin protein cages have provided impetus to focus on the methodical study of these self-assembling nanosystems. Among these proteins, Escherichia coli bacterioferritin (EcBfr), although architecturally very similar to other members of the family, shows structural instability...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi200207w
更新日期：2011-05-17 00:00:00
abstract：:Under conditions where muscle actin only partially polymerizes, or where it does not polymerize at all, a significant enhancement of polymerization was observed if equimolar phalloidin was also present. The increased extent of polymerization in the the presence of phalloidin can be explained by the reduced critical ac...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00507a006
更新日期：1981-02-17 00:00:00
abstract：:Sir2 protein deacetylases (or sirtuins) catalyze NAD+-dependent conversion of epsilon-amino-acetylated lysine residues to deacetylated lysine, nicotinamide, and 2'-O-acetyl-ADP-ribose. Small-molecule modulation of sirtuin activity might treat age-associated diseases, such as type II diabetes, obesity, and neurodegener...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi7013294
更新日期：2007-12-18 00:00:00
abstract：:A strategy to detect P-glycoprotein (P-gp) on cell membrane and quantify the cell number using electrochemical immunoassay was developed by effective surface immunoreactions and immobilization of cells on a highly hydrophilic interface, which was constructed by adsorption of colloidal gold nanoparticles on a methoxysi...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0507332
更新日期：2005-08-30 00:00:00
abstract：:Thermal and chemical modification of protein structures is known to affect their interfacial activity. We have looked in detail at the effect of heating on the structure and subsequently the surface properties of LTP1, a nonspecific lipid transfer protein from barley, both in the presence and in the absence of its lip...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi901328f
更新日期：2009-12-29 00:00:00
abstract：:Heme oxygenase (HO), from the pathogenic bacterium N. meningitidis(NmHO), which secures host iron, shares many properties with mammalian HOs but also exhibits some key differences. The crystal structure appears more compact, and the crystal-undetected C-terminus interacts with substrate in solution. The unique nature ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi200978g
更新日期：2011-10-18 00:00:00
abstract：:The substrate specificity of the human enzyme glutathione reductase was changed from its natural substrate glutathione to trypanothione [N1,N8-bis(glutathionyl)spermidine] by site-directed mutagenesis of two residues. The glutathione analogue, trypanothione, is the natural substrate for trypanothione reductase, an enz...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00239a006
更新日期：1991-06-25 00:00:00
abstract：:Vangl1 and Vangl2 are membrane proteins that play an important role in neurogenesis, and Vangl1/Vangl2 mutations cause neural tube defects in mice and humans. At the cellular level, Vangl proteins regulate the establishment of planar cell polarity (PCP), a process requiring membrane assembly of asymmetrically distribu...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi101767a
更新日期：2011-03-29 00:00:00
abstract：:The thermodynamic parameters characterizing the interaction between rabbit fast skeletal muscle troponin and tropomyosin have been determined at 25 degrees C for three solution conditions: buffer containing (A) 1 mM CaCl2, simulating a "turned-on" state, (B) 3 mM MgCl2, simulating a "turned-off" state, and (C) 2 mM et...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00340a040
更新日期：1985-09-10 00:00:00
abstract：:Some aminoacyl-tRNA synthetases (AARSs) employ an editing mechanism to ensure the fidelity of protein synthesis. Leucyl-tRNA synthetase (LeuRS), isoleucyl-tRNA synthetase (IleRS), and valyl-tRNA synthetase (ValRS) share a common insertion, called the CP1 domain, which is responsible for clearing misformed products. Th...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi702494q
更新日期：2008-04-22 00:00:00
abstract：:The complete sequence of rabbit antibody light-chain L 120 has been elucidated. The antibody was raised against Micrococcus lysodeikticus bacteria and is specific for the external part of the cell wall. All protein used in this work was obtained from a single 50-mL bleeding. The variable region of L 120 is compared to...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00613a031
更新日期：1978-10-03 00:00:00
abstract：:The gamma-aminobutyric acid transporter of rat brain synaptic vesicles was reconstituted in proteoliposomes, and its activity was studied in response to artificially created membrane potentials or proton gradients. Changes of the membrane potential were monitored using the dyes oxonol VI and 3,3'-diisopropylthiodicarb...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00115a009
更新日期：1991-12-24 00:00:00
abstract：:We have combined equilibrium and steered molecular dynamics (SMD) simulations with principal component and correlation analyses to probe the mechanism of allosteric regulation in imidazole glycerol phosphate (IGP) synthase. An evolutionary analysis of IGP synthase revealed a conserved network of interactions leading f...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi061708e
更新日期：2007-02-27 00:00:00
abstract：:Many naturally occurring RNA structures contain single mismatches. However, the algorithms currently used to predict RNA structure from sequence rely on a minimal set of data for single mismatches, most of which occur rather infrequently in nature. As a result, several approximations and assumptions are used to predic...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi701311c
更新日期：2007-11-20 00:00:00
abstract：:Membrane fusion induced by the hemagglutinin glycoprotein of influenza virus has been extensively characterized, but the mechanism whereby the protein achieves the merger of the viral and target membrane lipids remains enigmatic. Various lipid intermediate structures have been proposed, and the energies required for t...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00017a001
更新日期：1995-05-02 00:00:00
abstract：:L-alpha-Hydroxy acid oxidase (listed as EC 1.4.3.2, L-amino acid: O2 oxidoreductase) has been purified 100-fold from rat kidney to apparent homogeneity by gel electrophoresis. A subunit molecular weight of 47,500 was found by sodium dodecyl sulfate gel electrophoresis, but in contrast to previous reports, the enzyme h...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00683a005
更新日期：1975-06-17 00:00:00
abstract：:Bovine milk lipoprotein lipase (LpL) catalyzes the hydrolysis of the water-soluble esters p-nitrophenyl acetate (PNPA) and p-nitrophenyl butyrate (PNPB). The same protein and same active site are involved in hydrolysis of water-soluble p-nitrophenyl esters and emulsified trioleoylglycerol since (a) trioleoylglycerol h...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00269a038
更新日期：1982-12-21 00:00:00
abstract：:The quantitative tissue specificity of the high mobility group (HMG) chromosomal proteins was investigated. Perchloric acid (PCA) extracts of four different chicken tissues and erythrocytes contained three proteins which comigrated on NaDodSO4-polyacrylamide gels with the HMG's 1,2, and E from erythrocyte nuclei. Thes...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00560a003
更新日期：1980-09-16 00:00:00
abstract：:We have studied the hydrogen-exchange kinetics of hemoglobin A0 as a function of ligand, CO, saturation. In the noncooperative system, azide binding to methemoglobin, the alterations in exchange kinetics are proportional to the average degree of ligation. However, in the case of CO binding to deoxyhemoglobin the chang...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00297a015
更新日期：1984-01-17 00:00:00
abstract：:Human erythrocyte band 3 inhibits glycolytic enzymes, including aldolase, by binding these cytoplasmic enzymes at its N-terminus. Phosphorylation of Y8 disrupts inhibition, and there is evidence that in vivo glycolysis levels in erythrocytes are regulated in part by a phosphorylation/dephosphorylation signaling pathwa...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00051a005
更新日期：1995-12-26 00:00:00
abstract：:The active site structures of human Q31 granzyme A, murine granzymes (A, B, C, D, E, and F), and human granzymes (A, B, and 3) isolated from cytotoxic T lymphocytes (CTL) were studied with peptide thioester substrates, peptide chloromethyl ketone, and isocoumarin inhibitors. Human Q31, murine, and human granzyme A hyd...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00222a027
更新日期：1991-02-26 00:00:00
abstract：:Peripherin/rds is a 39 kDa integral membrane glycoprotein essential for normal photoreceptor cell development in vertebrates. It has been implicated in several human retinal degenerative diseases including retinitis pigmentosa and macular degeneration and is thought to play a structural role at photoreceptor outer seg...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00043a028
更新日期：1995-10-31 00:00:00
abstract：:X-ray crystallographic data have implicated Arg-292 as the residue responsible for the preferred side-chain substrate specificity of aspartate aminotransferase. It forms a salt bridge with the beta or gamma carboxylate group of the substrate [Kirsch, J. F., Eichele, G., Ford, G. C., Vincent, M. G., Jansonius, J. N., G...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00412a052
更新日期：1988-06-14 00:00:00
abstract：:The proton nuclear Overhauser effects of bovine alpha-lactalbumin were studied at 200 MHz by irradiation of an upfield ring current shifted methylene at -2.45 ppm (assigned to Ile-95) and two aromatic protons, Tyr-103 (8.36 ppm) and Trp-60 (5.85 ppm). The experimental results were consistent with a putative three-dime...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00346a035
更新日期：1985-12-03 00:00:00
abstract：:Activator of G protein signaling 3 (AGS3) activates the Gbetagamma mating pathway in yeast in a manner that is independent of heptahelical receptors. It competes with Gbetagamma subunits to bind GDP-bound Gi/o(alpha) subunits via four repeated G protein regulatory (GPR) domains in the carboxyl-terminal half of the mol...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi034561p
更新日期：2003-07-08 00:00:00
