Modulation of GLUT4 and GLUT1 recycling by insulin in rat adipocytes: kinetic analysis based on the involvement of multiple intracellular compartments.

abstract：:KCNE1 is a single transmembrane protein that modulates the function of voltage-gated potassium channels, including KCNQ1. Hereditary mutations in the genes encoding either protein can result in diseases such as congenital deafness, long QT syndrome, ventricular tachyarrhythmia, syncope, and sudden cardiac death. Despi...

journal_title：Biochemistry

authors： Sahu ID,Craig AF,Dunagan MM,Troxel KR,Zhang R,Meiberg AG,Harmon CN,McCarrick RM,Kroncke BM,Sanders CR,Lorigan GA

更新日期：2015-10-20 00:00:00

abstract：:CD4 is expressed on the surface of specific leukocytes where it plays a key role in the activation of immunostimulatory T-cells and acts as a primary receptor for HIV-1 entry. CD4 has four ecto-domains (D1-D4) of which D1, D2, and D4 contain disulfide bonds. Although disulfide bonds commonly serve structural or cataly...

journal_title：Biochemistry

authors： Owen GR,Channell JA,Forsyth VT,Haertlein M,Mitchell EP,Capovilla A,Papathanasopoulos M,Cerutti NM

更新日期：2016-04-19 00:00:00

abstract：:Eukaryotic initiation factor 5 (eIF5) plays multiple roles in translation initiation. Its N-terminal domain functions as a GTPase-activator protein (GAP) for GTP bound to eIF2, while its C-terminal region nucleates the interactions between multiple translation factors, including eIF1, which acts to inhibit GTP hydroly...

journal_title：Biochemistry

authors： Conte MR,Kelly G,Babon J,Sanfelice D,Youell J,Smerdon SJ,Proud CG

更新日期：2006-04-11 00:00:00

abstract：:The mechanism of binding of the substrates Mg x ATP and ribose 5-phosphate as well as Mg2+ to the enzyme 5-phospho-D-ribosyl (alpha-1-diphosphate synthetase from Escherichia coli has been analyzed. By use of the competive inhibitors of ATP and ribose 5-phosphate binding, alpha,beta-methylene ATP and (+)-1-alpha,2-alph...

journal_title：Biochemistry

authors： Willemoës M,Hove-Jensen B

更新日期：1997-04-22 00:00:00

abstract：:By following the kinetics of abortive and productive synthesis in single-round transcription assays, we confirm the existence of two general classes of initial transcribing complexes (ITCs), which we term "productive ITC" and "unproductive ITC". The productive ITCs are able to escape from the promoter rapidly to produ...

journal_title：Biochemistry

authors： Vo NV,Hsu LM,Kane CM,Chamberlin MJ

更新日期：2003-04-08 00:00:00

abstract：:We used a combination of optical and calorimetric techniques to investigate the incorporation of deoxythymidine --> deoxyuridine (dT --> dU) substitutions in the duplex and third strand of the parallel intramolecular triplex d(A(7)C(5)T(7)C(5)T(7)) (ATT). UV and differential scanning calorimetry melting experiments sh...

journal_title：Biochemistry

authors： Soto AM,Rentzeperis D,Shikiya R,Alonso M,Marky LA

更新日期：2006-03-07 00:00:00

abstract：:Telomeres terminate nearly exclusively in single-stranded DNA (ssDNA) overhangs comprised of the G-rich 3' end. This overhang varies widely in length from species to species, ranging from just a few bases to several hundred nucleotides. These overhangs are not merely a remnant of DNA replication but rather are the res...

journal_title：Biochemistry

authors： Lloyd NR,Dickey TH,Hom RA,Wuttke DS

更新日期：2016-09-27 00:00:00

abstract：:DNA containing short sequences of the form (dA)n.(dT)n can exhibit pronounced degrees of stable curvature of the helix axis, provided that these homooligomeric stretches are approximately in phase with the helix repeat. However, the precise origin of this effect is unknown. We have observed that pyrimidine 5-methyl gr...

journal_title：Biochemistry

authors： Hagerman PJ

更新日期：1990-02-27 00:00:00

abstract：:Molecular dynamics simulations employing a molecular mechanics (MM) force field and hybrid quantum mechanics (QM) and MM (QM/MM) have been carried out to investigate the product specificity and mechanism of the histone H4 lysine 20 (H4-K20) methylation by human histone lysine methyltransferase SET8. At neutral pH, the...

journal_title：Biochemistry

authors： Zhang X,Bruice TC

更新日期：2008-06-24 00:00:00

abstract：:Apolipoprotein B (apoB) is a nonexchangeable apolipoprotein that dictates the synthesis of chylomicrons and very low density lipoproteins. ApoB is the major protein in low density lipoprotein, also known as the "bad cholesterol" that is directly implicated in atherosclerosis. It has been suggested that the N-terminal ...

journal_title：Biochemistry

authors： Jiang ZG,Gantz D,Bullitt E,McKnight CJ

更新日期：2006-10-03 00:00:00

abstract：:T4 RNA ligase catalyzes the ATP-dependent addition of a single 2'-deoxyribonucleoside 3',5'-bisphosphate to the 3'-hydroxyl of an oligodeoxyribonucleotide. The bisphosphate is joined to the deoxyoligomer by a 3' leads to 5' phosphodiester bond and the product, which is terminated by a 3'-phosphate, is one nucleotide l...

journal_title：Biochemistry

authors： Hinton DM,Baez JA,Gumport RI

更新日期：1978-11-28 00:00:00

abstract：:F62 LOS of Neisseria gonorrhoeae consists of two major LOS components; the higher and smaller molecular weight (MW) components were recognized by MAbs 1-1-M and 3F11 respectively. Base-line separation of the two major oligosaccharide (OS) components from F62 LOS was achieved by Bio-Gel P-4 chromatography after dephosp...

journal_title：Biochemistry

authors： Yamasaki R,Bacon BE,Nasholds W,Schneider H,Griffiss JM

更新日期：1991-10-29 00:00:00

abstract：:In this study, we prepared several shortened and full-length insulin analogues with substitutions at position B26. We compared the binding affinities of the analogues for rat adipose membranes with their ability to lower the plasma glucose level in nondiabetic Wistar rats in vivo after subcutaneous administration, and...

journal_title：Biochemistry

authors： Záková L,Kazdová L,Hanclová I,Protivínská E,Sanda M,Budesínský M,Jirácek J

更新日期：2008-05-27 00:00:00

abstract：:The active site topography of rabbit CYP4B1 has been studied relative to CYP2B1 and CYP102 using a variety of aromatic probe substrates. Oxidation of the prochiral substrate cumene by CYP4B1, but not CYP2B1 or CYP102, resulted in the formation of the thermodynamically disfavored omega-hydroxy metabolite, 2-phenyl-1-pr...

journal_title：Biochemistry

authors： Henne KR,Fisher MB,Iyer KR,Lang DH,Trager WF,Rettie AE

更新日期：2001-07-24 00:00:00

abstract：:The classical complement pathway, which plays a vital role in preventing infection, is initiated by the action of the serine proteases C1r and C1s. We have examined the hydrolysis of substrates representing cleavage sequences in the physiological substrates for C1s, C2 and C4. These studies showed that the P(1)'-P(4)'...

journal_title：Biochemistry

authors： O'Brien G,Quinsey NS,Whisstock JC,Pike RN

更新日期：2003-12-23 00:00:00

abstract：:Monomeric sarcosine oxidase (MSOX) contains covalently bound FAD and catalyzes the oxidation of sarcosine (N-methylglycine) and other secondary amino acids, including L-proline. The reductive half-reaction with L-proline proceeds via a rapidly attained equilibrium (K(d)) between free E(ox) and the E(ox).S complex, fol...

journal_title：Biochemistry

authors： Zhao G,Jorns MS

更新日期：2002-08-06 00:00:00

abstract：:RecA-catalyzed DNA recombination is initiated by a mandatory, high-energy form of DNA in RecA-nucleoprotein filaments, where bases are highly unstacked and the backbone is highly unwound. Interestingly, only the energetics consequent to adenosine triphosphate (ATP) binding, rather than its hydrolysis, seems sufficient...

journal_title：Biochemistry

authors： Ramreddy T,Sen S,Rao BJ,Krishnamoorthy G

更新日期：2003-10-21 00:00:00

abstract：:Dodecameric glutamine synthetase (GS) from bacteria is formed from two face-to-face hexameric rings of identical subunits. These highly symmetrical aggregates from some bacteria, including Escherichia coli, "stack" in the presence of Zn2+ and other divalent ions to generate protein tubes (phase I) and subsequently ass...

journal_title：Biochemistry

authors： Yanchunas J Jr,Dabrowski MJ,Schurke P,Atkins WM

更新日期：1994-12-20 00:00:00

abstract：:Nickel is a fundamental micronutrient for cellular life, but it is toxic in soluble form at nonphysiological concentrations. Such potentially contradictory features required living organisms to develop efficient systems for nickel utilization and homeostasis. This is the case for incorporation of nickel into the activ...

journal_title：Biochemistry

authors： Zambelli B,Musiani F,Savini M,Tucker P,Ciurli S

更新日期：2007-03-20 00:00:00

abstract：:The amino acid sequence of human alpha-fetoprotein, a 67-kDa protein present in mammalian embryonic serum, was verified by fast atom bombardment mass spectrometric (FAB/MS) analyses of three different enzymatic digests of the protein. Human alpha-fetoprotein obtained from a large-scale cell culture was digested with t...

journal_title：Biochemistry

authors： Pucci P,Siciliano R,Malorni A,Marino G,Tecce MF,Ceccarini C,Terrana B

更新日期：1991-05-21 00:00:00

abstract：:2-[(R)-2-Hydroxypropylthio]ethanesulfonate (R-HPC) dehydrogenase (DH) catalyzes the reversible oxidation of R-HPC to 2-(2-ketopropylthio)ethanesulfonate (2-KPC) in a key reaction in the bacterial conversion of chiral epoxides to beta-keto acids. R-HPCDH is highly specific for the R-enantiomer of HPC, while a separate ...

journal_title：Biochemistry

authors： Clark DD,Boyd JM,Ensign SA

更新日期：2004-06-01 00:00:00

abstract：:Propeptide processing occurs in specific compartments of the secretory pathway, but how these processing-competent organelles are generated from their processing-incompetent precursor compartments is unknown. To dissect the process biochemically, we have developed a novel cell-free system reconstituting the production...

journal_title：Biochemistry

authors： Andresen JM,Moore HP

更新日期：2001-10-30 00:00:00

abstract：:A 39/34-kilodalton (kDa) monomeric dispase fragment of von Willebrand factor (vWF) has been purified by heparin affinity chromatography. Detailed structural analysis of the individual 39- and 34-kDa fragments indicated that they had identical amino acid sequences extending from Leu-480/Val-481 to Gly-718 with an intra...

journal_title：Biochemistry

authors： Andrews RK,Gorman JJ,Booth WJ,Corino GL,Castaldi PA,Berndt MC

更新日期：1989-10-17 00:00:00

abstract：:In the yeast Saccharomyces cerevisiae, CTP synthetase [EC 6.3.4.2; UTP:ammonia ligase (ADP-forming)] is the product of the URA7 gene. CTP synthetase was purified 503-fold to apparent homogeneity from cells bearing the URA7 gene on a multicopy plasmid that directed a 10-fold overproduction of the enzyme. The purificati...

journal_title：Biochemistry

authors： Yang WL,McDonough VM,Ozier-Kalogeropoulos O,Adeline MT,Flocco MT,Carman GM

更新日期：1994-09-06 00:00:00

abstract：:The cyanobacterium Synechocystis sp. PCC 6803 (S6803) expresses a two-on-two globin in which His46 (distal side) and His70 (proximal) function as heme iron axial ligands. His46 can be displaced by O2, CO, and CN-, among others, whereas His70 is not labile under native conditions. The residue preceding the proximal his...

journal_title：Biochemistry

authors： Knappenberger JA,Kuriakose SA,Vu BC,Nothnagel HJ,Vuletich DA,Lecomte JT

更新日期：2006-09-26 00:00:00

abstract：:Oligopeptides and dipeptides are transported into Saccharomyces cerevisiae by a carrier-mediated system. In the dark, leucyl-p-nitroanilide (Leu-p-NA) and leucyl-leucyl-4-azido-2-nitrophenylalanine [Leu-Leu-Phe-(4N3,2NO2)] are competitive inhibitors of peptide transport by S. cerevisiae cells. The photolysis of yeast ...

journal_title：Biochemistry

authors： Becker JM,Dunsmore KP,Steinfeld AS,Naider F

更新日期：1982-11-09 00:00:00

abstract：:We find that the isolated, extracellular domain of tissue factor (TF1-218; sTF) exhibits only 4% of the activity of wild-type transmembrane TF (TF1-263) in an assay that measures the conversion of factor X to Xa by the TF:VIIa complex. Further, the activity of sTF is manifest only when vesicles consisting of phosphati...

journal_title：Biochemistry

authors： Waxman E,Ross JB,Laue TM,Guha A,Thiruvikraman SV,Lin TC,Konigsberg WH,Nemerson Y

更新日期：1992-04-28 00:00:00

abstract：:DrrA and DrrB proteins form an ATP-dependent efflux pump for doxorubicin and daunorubicin in Streptomyces peucetius. DrrA, the catalytic subunit, forms a complex with the integral membrane protein DrrB. Previous studies have provided evidence for strong interaction between these two proteins, which was found to be cri...

journal_title：Biochemistry

authors： Rao DK,Kaur P

更新日期：2008-03-04 00:00:00

abstract：:DNA sequences containing homopurine d(G1-3A)n tracts are known to be capable of adopting non-B-DNA conformations. The structural polymorphism of these sequences is a direct consequence of the structural properties of the homopurine d(G1-3A)n tracts. Depending on the conditions, d(GA)n DNA sequences can form antiparall...

journal_title：Biochemistry

authors： Huertas D,Azorín F

更新日期：1996-10-08 00:00:00

abstract：:The Sir2 enzyme family is responsible for a newly classified chemical reaction, NAD(+)-dependent protein deacetylation. New peptide substrates, the reaction mechanism, and the products of the acetyl transfer to NAD(+) are described for SIR2. The final products of SIR2 reactions are the deacetylated peptide and the 2' ...

journal_title：Biochemistry

authors： Sauve AA,Celic I,Avalos J,Deng H,Boeke JD,Schramm VL

更新日期：2001-12-25 00:00:00