Abstract:
:Monomeric sarcosine oxidase (MSOX) contains covalently bound FAD and catalyzes the oxidation of sarcosine (N-methylglycine) and other secondary amino acids, including L-proline. The reductive half-reaction with L-proline proceeds via a rapidly attained equilibrium (K(d)) between free E(ox) and the E(ox).S complex, followed by a practically irreversible reduction step (E(ox).S --> E(red).P) associated with a rate constant, k(lim). The effect of pH on the reductive half-reaction shows that the K(d) for L-proline binding is pH-independent (pH 6.46-9.0). This indicates that MSOX binds the zwitterionic form of L-proline, the predominant species in solution at neutral pH (pK(a) = 10.6). Values for the limiting rate of reduction (k(lim)) are, however, strongly pH-dependent and indicate that an ionizable group in the E(ox).L-proline complex (pK(a) = 8.02) must be unprotonated for conversion to E(red).P. Charge-transfer interaction with L-proline as the donor and FAD as acceptor is possible only with the anionic form of L-proline. The ionizable group in the E(ox).L-proline complex is required for conversion of enzyme-bound L-proline from the zwitterionic to the reactive anionic form, as judged by the independently determined pK(a) for charge-transfer complex formation with the MSOX flavin (pK(a) = 7.94). The observation that the anionic form of L-proline with a neutral amino group is the reactive species in the reduction of MSOX is similar to that observed for other flavoenzymes that oxidize amines, including monoamine oxidase and trimethylamine dehydrogenase.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Zhao G,Jorns MSdoi
10.1021/bi020285nsubject
Has Abstractpub_date
2002-08-06 00:00:00pages
9747-50issue
31eissn
0006-2960issn
1520-4995pii
bi020285njournal_volume
41pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract::The primary structure of the 43-kilodalton peripheral membrane protein (43-kDa protein) of Torpedo nicotinic postsynaptic membrane has been determined. The 43-kDa protein, which was isolated by preparative sodium dodecyl sulfate-polyacrylamide gel electrophoresis, has an amino terminus resistant to Edman degradation, ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00396a034
更新日期:1987-11-03 00:00:00
abstract::A conformational transition is described for the polypeptide, gramicidin A, in which a dimer that forms a left-handed intertwined antiparallel helix is converted to a single-stranded amino terminus to amino terminus right-handed helix. The starting structure is determined here by solution NMR methods while reference i...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00152a019
更新日期:1992-09-22 00:00:00
abstract::The effects of phosphorylation at Ser40 of rat tyrosine hydroxylase on the affinities of catechols have been determined with both the ferric and ferrous forms of the enzyme. Phosphorylation had no effect on the Ki value for the inhibition of the ferrous enzyme by either dopamine or DOPA when the initial rate of turnov...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi980582l
更新日期:1998-06-23 00:00:00
abstract::Methylmalonyl-CoA mutase from Propionibacterium shermanii is an adenosylcobalamin-dependent enzyme which catalyzes the reversible isomerization of methylmalonyl-CoA and succinyl-CoA. The rate of tritium loss from 5'-[3H]adenosylcobalamin during the enzymic reaction and the relative rates of tritium appearance in subst...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi961250o
更新日期:1996-09-10 00:00:00
abstract::N-Ethylmaleimide modified heavy meromyosin in only 3-fold activated by actin rather than 200-fold as is normal heavy meromyosin (Silverman, R., Eisenberg, E., and Kielley, W. W. (1972), Nature (London) 240, 207). Ultracentrifuge studies demonstrated that in the absence of ATP the N-ethylmaleimide modified heavy merom...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00688a020
更新日期:1975-08-26 00:00:00
abstract::Carboxylation of vitamin K-dependent (VKD) proteins is required for their activity and depends on reduced vitamin K generated by vitamin K oxidoreductase (VKOR) and a redox protein that regenerates VKOR activity. VKD protein carboxylation is inefficient in mammalian cells, and to understand why carboxylation becomes s...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi051986y
更新日期:2006-05-02 00:00:00
abstract::Nonribosomal peptide synthetases (NRPSs) are megasynthetases that require complex and specific interactions between multiple domains and proteins to functionally produce a metabolite. MbtH-like proteins (MLPs) are integral components of many NRPSs and interact directly with the adenylation domain of the megasynthetase...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.8b00552
更新日期:2018-07-17 00:00:00
abstract::Peripherin/rds is a 39 kDa integral membrane glycoprotein essential for normal photoreceptor cell development in vertebrates. It has been implicated in several human retinal degenerative diseases including retinitis pigmentosa and macular degeneration and is thought to play a structural role at photoreceptor outer seg...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00043a028
更新日期:1995-10-31 00:00:00
abstract::Directed hydroxyl radical probing of 16S ribosomal RNA from Fe(II) tethered to specific sites within the RNA was used to determine RNA-RNA proximities in 70S ribosomes. We have transcribed 16S ribosomal RNA in vitro as two separate fragments, covalently attached an Fe(II) probe to a 5'-guanosine-alpha-phosphorothioate...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi981644a
更新日期:1999-01-19 00:00:00
abstract::Assembly of polymerase chain reactions at room temperature can sometimes lead to low yields or unintentional products due to mispriming. Mutation of isoleucine 707 to leucine in DNA polymerase I from Thermus aquaticus substantially decreases its activity at room temperature without compromising its ability to amplify ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi501198f
更新日期:2015-01-27 00:00:00
abstract::Cellular Ca2+ transients and Ca2+-binding proteins regulate physiological phenomena as diverse as muscle contraction, neurosecretion, and cell division. When Ca2+ is rapidly mixed with slow Ca2+ chelators, EGTA, or Mg2+/EDTA, artificial Ca2+ transients (ACTs) of varying duration (0.1-50 ms half-widths (hws)) and ampli...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi982495z
更新日期:1999-03-30 00:00:00
abstract::Many strains of Campylobacter jejuni display modified heptose residues in their capsular polysaccharides (CPS). The precursor heptose was previously shown to be GDP-d-glycero-α-d-manno-heptose, from which a variety of modifications of the sugar moiety have been observed. These modifications include the generation of 6...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.0c00096
更新日期:2020-04-07 00:00:00
abstract::Human phenylalanine hydroxylase is a liver-specific enzyme that catalyzes the conversion of phenylalanine to tyrosine. Absence of enzymatic activity results in phenylketonuria, a genetic disorder that causes development of severe mental retardation in untreated children. In this paper we report the cloning and structu...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00352a001
更新日期:1986-02-25 00:00:00
abstract::Prion diseases are associated with a conformational switch in the prion protein (PrP) from its normal cellular form (denoted PrP(C)) to a disease-associated "scrapie" form (PrP(Sc)). A number of PrP(Sc)-like conformations can be generated by incubating recombinant PrP(C) at low pH, indicating that protonation of key r...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi100572j
更新日期:2010-10-12 00:00:00
abstract::Indolethylamine-N-methyltransferase (INMT) is a Class 1 transmethylation enzyme known for its production of N,N-dimethyltryptamine (DMT), a hallucinogen with affinity for various serotonergic, adrenergic, histaminergic, dopaminergic, and sigma-1 receptors. DMT is produced via the action of INMT on the endogenous subst...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi500175p
更新日期:2014-05-13 00:00:00
abstract::Apolipoprotein AIV is one of the principal apolipoproteins synthesized by the rat small intestine. We have cloned a cDNA encoding a portion of preapolipoprotein AIV mRNA. A kinetically fractionated cDNA probe highly enriched for the abundant intestinal mucosal mRNA sequences was used to screen a library of recombinant...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00265a007
更新日期:1982-10-26 00:00:00
abstract::The X-ray crystal structure of human soluble epoxide hydrolase (sEH) has been determined at 2.6 A resolution, revealing a domain-swapped quaternary structure identical to that observed for the murine enzyme [Argiriadi, M. A., Morisseau, C., Hammock, B. D., and Christianson, D. W. (1999) Proc. Natl. Acad. Sci. U.S.A. 9...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi036189j
更新日期:2004-04-27 00:00:00
abstract::The structure of a short-chain IgG2a antibody, which is a member of the family of mouse anti-dansyl switch variant antibodies with identical variable regions but different heavy-chain constant regions [Dangl, J.L., Parks, D. R., Oi, V. T., & Herzenberg, L. A. (1982) Cytometry 2, 395-401], is reported. Amino acid seque...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00476a013
更新日期:1990-06-19 00:00:00
abstract::The cytological architecture of the synaptonemal complex (SC), a meiosis-specific proteinaceous structure, is evolutionarily conserved among eukaryotes. However, little is known about the biochemical properties of SC components or the mechanisms underlying their roles in meiotic chromosome synapsis and recombination. ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi4005528
更新日期:2013-08-06 00:00:00
abstract::The fetal Alz-50 reactive clone 1 (FAC1) protein exhibits altered expression and subcellular localization during neuronal development and neurodegenerative diseases such as Alzheimer's disease. Using the yeast two-hybrid screen, the human orthologue of Keap1 (hKeap1) was identified as a FAC1 interacting protein. Keap1...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0494166
更新日期:2004-09-28 00:00:00
abstract::Site-directed mutagenesis studies have confirmed that the distal histidine in myoglobin stabilizes bound O2 by hydrogen bonding and have suggested that it is the polar character of the imidazole side chain rather than its size that limits the rate of ligand entry into the protein. We constructed an isosteric Val68 to ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00239a025
更新日期:1991-06-25 00:00:00
abstract::Phytochromes are widespread red/far-red photosensory proteins well known as critical regulators of photomorphogenesis in plants. It is often assumed that natural selection would have optimized the light sensing efficiency of phytochromes to minimize nonproductive photochemical deexcitation pathways. Surprisingly, the ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi500108s
更新日期:2014-05-06 00:00:00
abstract::Oligonucleotides capable of sequence-specific triple helix formation have been proposed as DNA binding ligands useful for modulation of gene expression and for directed genome modification. However, the effectiveness of such triplex-forming oligonucleotides (TFOs) depends on their ability to bind to their target sites...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi982290q
更新日期:1999-02-09 00:00:00
abstract::Prodan [6-propionyl-2-(dimethylamine)naphthalene] is a hydrophobic fluorescent probe which is extremely sensitive to both the polarity and the hydrogen-bond donating capacity of the solvent. In binary mixtures of solvents, the hydrogen-bond donating effect on Prodan fluorescence saturates at relatively low concentrati...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00154a021
更新日期:1992-10-06 00:00:00
abstract::Previously we showed that the low-molecular-weight mucin (MG2, encoded by MUC7), a major component of human submandibular/sublingual saliva, is a bacterial receptor that coats the tooth surface. Here we tested the hypothesis that the structure of its carbohydrate residues contains important information about its funct...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi972612a
更新日期:1998-04-07 00:00:00
abstract::Defects in the MMACHC gene represent the most common disorder of cobalamin (Cbl) metabolism, affecting synthesis of the enzyme cofactors adenosyl-Cbl and methyl-Cbl. The encoded MMACHC protein binds intracellular Cbl derivatives with different upper axial ligands and exhibits flavin mononucleotide (FMN)-dependent decy...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi300150y
更新日期:2012-06-26 00:00:00
abstract::Exoenzyme S (ExoS) is a bifunctional virulence factor directly translocated into eukaryotic cells by the type III secretory process of Pseudomonas aeruginosa. Bacterial translocation of ExoS into epithelial cells is associated with diverse effects on cell function, including inhibition of growth, alterations in cell m...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi025826n
更新日期:2002-07-30 00:00:00
abstract::A soluble Ca2+/calmodulin-dependent protein kinase has been purified from rat brain to near homogeneity by using casein as substrate. The enzyme was purified by using hydroxylapatite adsorption chromatography, phosphocellulose ion-exchange chromatography, Sepharose 6B gel filtration, affinity chromatography using calm...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00318a018
更新日期:1984-11-06 00:00:00
abstract::The pH-dependent behavior of the ferric forms of two soluble truncations of Rhizobium meliloti FixL, FixL (heme and kinase domains, functional), and FixLN (heme domain) are examined by UV-visible, resonance Raman, and electron paramagnetic resonance spectroscopy. Global analysis of UV-visible data indicates that the p...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi981439v
更新日期:1998-09-29 00:00:00
abstract::Snapshots of three consecutive steps in the proteolytic reaction of HIV-1 protease (PR) were obtained in crystal structures at resolutions of 1.2-1.4 Å. Structures of wild-type protease and two mutants (PR(V32I) and PR(I47V)) with V32I and I47V substitutions, which are common in drug resistance, reveal the gem-diol te...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi3008092
更新日期:2012-10-02 00:00:00