A network of conserved interactions regulates the allosteric signal in a glutamine amidotransferase.

abstract：:P1,P2-Bidentate Co(NH3)4PP was found to be a competitive inhibitor of pyrophosphatase vs. MgPP (Kis = 8.7 mM, pH 7) and, in the presence of Mg2+, an active substrate as well. P1,P2-Bidentate Cr(III) complexes of pyrophosphate, imidodiphosphate, and methylenediphosphonate were also competitive inhibitors vs. MgPP (pH 5...

journal_title：Biochemistry

authors： Knight WB,Fitts SW,Dunaway-Mariano D

更新日期：1981-07-07 00:00:00

abstract：:The complex of tropomyosin and troponin binds to actin and inhibits activation of myosin ATPase activity and force production of striated muscles at low free Ca(2+) concentrations. Ca(2+) stimulates ATP activity, and at subsaturating actin concentrations, the binding of NEM-modified S1 to actin-tropomyosin-troponin in...

journal_title：Biochemistry

authors： Gafurov B,Fredricksen S,Cai A,Brenner B,Chase PB,Chalovich JM

更新日期：2004-12-07 00:00:00

abstract：:RecG is a DNA helicase involved in the repair of damage at a replication fork and catalyzes the reversal of the fork to a point beyond the damage in the template strand. It unwinds duplex DNA in reactions that are coupled to ATP hydrolysis. The kinetic mechanism of duplex DNA unwinding by RecG was analyzed using a qua...

journal_title：Biochemistry

authors： Martinez-Senac MM,Webb MR

更新日期：2005-12-27 00:00:00

abstract：:To date, little work has been conducted on the relationship between solute and buffer molecules and conformational exchange motion in enzymes. This study uses solution NMR to examine the effects of phosphate, sulfate, and acetate in comparison to MES- and HEPES-buffered references on the chemical shift perturbation an...

journal_title：Biochemistry

authors： Wong M,Khirich G,Loria JP

更新日期：2013-09-17 00:00:00

abstract：:Filamins are a family of actin cross-linking proteins that are primarily localized in the cortical cytoplasm of all mammalian cells. Until now, three major isoforms (filamins a, b, and c) have been identified, that were shown to be differentially expressed and/or localized in different tissues. An amino-terminal doubl...

journal_title：Biochemistry

authors： Himmel M,Van Der Ven PF,Stöcklein W,Fürst DO

更新日期：2003-01-21 00:00:00

abstract：:In this study, we have investigated the effect of hydrophobic mismatch between the thickness of the membrane and a transmembrane segment of a protein that directly inserts into the membrane bilayer. For this purpose we used mutants of the single-spanning Pf3 coat protein that can spontaneously insert into Escherichia ...

journal_title：Biochemistry

authors： Ridder AN,van de Hoef W,Stam J,Kuhn A,de Kruijff B,Killian JA

更新日期：2002-04-16 00:00:00

abstract：:The primary subsite specificities of human leukocyte elastase, cathepsin G, porcine pancreatic elastase, rat mast cell proteases I and II, bovine chymotrypsin A alpha, and the protease from strain V-8 of Staphylococcus aureus have been mapped with a series of tripeptide thiobenzyl ester substrates of the general formu...

journal_title：Biochemistry

authors： Harper JW,Cook RR,Roberts CJ,McLaughlin BJ,Powers JC

更新日期：1984-06-19 00:00:00

abstract：:The dual specificity mitogen-activated protein kinase phosphatase MKP3 downregulates mitogenic signaling through dephosphorylation of extracellular signal-regulated kinase (ERK). Like other MKPs, MKP3 consists of a noncatalytic N-terminal domain and a catalytic C-terminal domain. ERK binding to the N-terminal noncatal...

journal_title：Biochemistry

authors： Kim Y,Rice AE,Denu JM

更新日期：2003-12-30 00:00:00

abstract：:The precipitation mechanism of alpha-globulin in the presence of myo-inositol hexaphosphate (sodium phytate) was studied in detail. The maximum interaction was found at pH 2.3 where the protein was in a dissociated state having an 8.3S aggregate and a 1.5S monomer. This interaction was predominantly dependent upon the...

journal_title：Biochemistry

authors： Rajendran S,Prakash V

更新日期：1993-04-06 00:00:00

abstract：:Femtosecond spectroscopy was performed on CO-liganded (fully reduced and mixed-valence states) and O(2)-liganded quinol oxidase bd from Escherichia coli. Substantial polarization effects, unprecedented for optical studies of heme proteins, were observed in the CO photodissociation spectra, implying interactions betwee...

journal_title：Biochemistry

authors： Borisov VB,Liebl U,Rappaport F,Martin JL,Zhang J,Gennis RB,Konstantinov AA,Vos MH

更新日期：2002-02-05 00:00:00

abstract：:The activity of peptidyl-tRNALys-CpCp2'dA was measured in an in vitro poly(A)-dependent polypeptide synthesizing system derived from Escherichia coli. It has already been shown that Lys-tRNALys-CpCp2'dA is active as an acceptor and Ac2-Lys-tRNALys-Cp2'dA can donate its peptidyl residue but that the overall poly(A)-dep...

journal_title：Biochemistry

authors： Wagner T,Sprinzl M

更新日期：1983-01-04 00:00:00

abstract：:Human Monocyte Chemotactic Protein (MCP)-2 has originally been isolated from stimulated osteosarcoma cells as a chemokine coproduced with MCP-1 and MCP-3. Here, a 5'-end extended MCP-2 cDNA was cloned from a human testis cDNA library. It encoded a 76 residue MCP-2 protein, but differed from the reported bone marrow-de...

journal_title：Biochemistry

authors： Van Coillie E,Proost P,Van Aelst I,Struyf S,Polfliet M,De Meester I,Harvey DJ,Van Damme J,Opdenakker G

更新日期：1998-09-08 00:00:00

abstract：:Stimulation of intracellular cholesterol esterification, which is catalyzed by the enzyme acyl-CoA:cholesterol O-acyltransferase (ACAT), by atherogenic lipoproteins in macrophages is a key step in the development of atheroma foam cells. Since other aspects of intracellular cholesterol metabolism involve proteolytic re...

journal_title：Biochemistry

authors： Schissel SL,Beatini N,Zha X,Maxfield FR,Tabas I

更新日期：1995-08-22 00:00:00

abstract：:The structure of the ca. 250 amino acid hormone binding domain of the human estrogen receptor (hER-LBD), expressed in E. coli and purified as a complex with estradiol, has been probed by selective proteolysis, with analysis of the protein fragments both by classical methods (SDS-PAGE and Edman N-terminal sequencing) a...

journal_title：Biochemistry

authors： Seielstad DA,Carlson KE,Kushner PJ,Greene GL,Katzenellenbogen JA

更新日期：1995-10-03 00:00:00

abstract：:The effect of anions on the stability of different functional conformations of Hb is examined through the determination of the dependence of O(2) affinity on water activity (a(w)). The control of a(w) is effected by varying the sucrose osmolal concentration in the bathing solution according to the "osmotic stress" met...

journal_title：Biochemistry

authors： Colombo MF,Seixas FA

更新日期：1999-09-07 00:00:00

abstract：:Voltage-dependent anion channel 1 (VDAC1) situated in the outer mitochondrial membrane regulates the transfer of various metabolites and is a key player in mitochondria-mediated apoptosis. Although many small chemicals that modulate the functions of VDAC1 have been reported to date, most, if not all, of them cannot be...

journal_title：Biochemistry

authors： Unten Y,Murai M,Yamamoto T,Watanabe A,Ichimaru N,Aburaya S,Aoki W,Shinohara Y,Miyoshi H

更新日期：2019-02-26 00:00:00

abstract：:The C2A domain of synaptotagmin 7 (Syt7) is a Ca(2+) and membrane binding module that docks and inserts into cellular membranes in response to elevated intracellular Ca(2+) concentrations. Like other C2 domains, Syt7 C2A binds Ca(2+) and membranes primarily through three loop regions; however, it docks at Ca(2+) conce...

journal_title：Biochemistry

authors： Chon NL,Osterberg JR,Henderson J,Khan HM,Reuter N,Knight JD,Lin H

更新日期：2015-09-22 00:00:00

abstract：:Exoenzyme S (ExoS) is a mono-ADP-ribosyltransferase secreted by the opportunistic pathogen Pseudomonas aeruginosa. ExoS requires a eukaryotic factor, the 14-3-3 protein, for enzymatic activity. Here, two aspects of the activation of the ADP-ribosyltransferase activity of ExoS by 14-3-3 proteins are examined. Initial s...

journal_title：Biochemistry

authors： Zhang L,Wang H,Masters SC,Wang B,Barbieri JT,Fu H

更新日期：1999-09-14 00:00:00

abstract：:Actin interaction with L-plastin, a plastin/fimbrins isoform of the alpha-actinin family of molecules, is poorly characterized, from the biochemical point of view. Besides, molecular modeling of the T-isoform has recently provided a complete model of interaction with filamentous actin [Volkmann, N., DeRosier, D., Mats...

journal_title：Biochemistry

authors： Lebart MC,Hubert F,Boiteau C,Ventéo S,Roustan C,Benyamin Y

更新日期：2004-03-09 00:00:00

abstract：:The interaction of poly(N epsilon, N epsilon, N epsilon-trimethyl-L-lysine) ([Lys(Me3)]n) and poly(N delta, N delta, N delta-trimethyl-L-ornithine) ([Orn(Me3)]n) with polynucleotides was studied by thermal denaturation, viscosity, and dissociation by salt. The methylated polymers decrease the viscosity of DNA in propo...

journal_title：Biochemistry

authors： Granados EN,Bello J

更新日期：1980-07-08 00:00:00

abstract：:MreB is a bacterial orthologue of actin that affects cell shape, polarity, and chromosome segregation. Although a significant body of work has explored its cellular functions, we know very little about the biochemical behavior of MreB. We have cloned, overexpressed in Escherichia coli, and purified untagged MreB1 from...

journal_title：Biochemistry

authors： Bean GJ,Amann KJ

更新日期：2008-01-15 00:00:00

abstract：:Most of the molybdoenzymes contain, in the amino-terminal region of their catalytic subunits, a conserved Cys group that in some cases binds an [Fe-S] cluster. In dissimilatory nitrate reductases, the first Cys residue of this motif is replaced by a conserved His residue. Site-directed mutagenesis of this residue (His...

journal_title：Biochemistry

authors： Magalon A,Asso M,Guigliarelli B,Rothery RA,Bertrand P,Giordano G,Blasco F

更新日期：1998-05-19 00:00:00

abstract：:Silk fibroin exists in a number of different states, such as silk I and silk II, with different properties largely defined by differences in secondary structure composition. Numerous attempts have been made to control the transitions from silk I to silk II in vitro to produce high-performance materials. Of all the fac...

journal_title：Biochemistry

authors： Zhou P,Xie X,Knight DP,Zong XH,Deng F,Yao WH

更新日期：2004-09-07 00:00:00

abstract：:Aldehyde dehydrogenase 7A1 (ALDH7A1) is part of lysine catabolism and catalyzes the NAD(+)-dependent oxidation of α-aminoadipate semialdehyde to α-aminoadipate. Herein, we describe a structural study of human ALDH7A1 focused on substrate recognition. Five crystal structures and small-angle X-ray scattering data are re...

journal_title：Biochemistry

authors： Luo M,Tanner JJ

更新日期：2015-09-08 00:00:00

abstract：:Sonication of lysophosphatidylcholine (lysoPC; 20 mumol/mL) and cholesterol (chol) in aqueous medium produces lamellar structures over a wide range of concentrations. From 25 to 47 mol % cholesterol, electron microscopy (EM) after negative staining showed extended stacklike lamellae about 40 A thick. From 50 to 60 mol...

journal_title：Biochemistry

authors： Kumar VV,Anderson WH,Thompson EW,Malewicz B,Baumann WJ

更新日期：1988-01-12 00:00:00

abstract：:Six active site mutants of Escherichia coli phosphofructokinase have been constructed and characterized using steady-state kinetics. All but one of the mutants (ES222) have significantly lower maximal activity, implicating these residues in the catalytic process. Replacement of Asp127, the key catalytic residue in the...

journal_title：Biochemistry

authors： Berger SA,Evans PR

更新日期：1992-09-29 00:00:00

abstract：:Organophosphate flame retardants are used to inhibit combustion and increase plasticity in plastics and durable foams. While not neurotoxic, these compounds are potential carcinogens, endocrine disrupters, and developmental toxins. The phosphotriesterase from Sphingobium sp. TCM1 (Sb-PTE) is unique among phosphotriest...

journal_title：Biochemistry

authors： Bigley AN,Narindoshvili T,Xiang DF,Raushel FM

更新日期：2020-03-31 00:00:00

abstract：:Genetic knockout of the BtR4 gene encoding the Heliothis virescens cadherin-like protein (HevCaLP) is linked to resistance against Cry1Ac toxin from Bacillus thuringiensis. However, the functional Cry1Ac receptor role of this protein has not been established. We previously proposed HevCaLP as a shared binding site for...

journal_title：Biochemistry

authors： Jurat-Fuentes JL,Adang MJ

更新日期：2006-08-15 00:00:00

abstract：:UreE is a homodimeric metallo-chaperone that assists the insertion of Ni(2+) ions in the active site of urease. The crystal structures of UreE from Bacillus pasteurii and Klebsiella aerogenes have been determined, but the details of the nickel-binding site were not elucidated due to solid-state effects that caused dis...

journal_title：Biochemistry

authors： Stola M,Musiani F,Mangani S,Turano P,Safarov N,Zambelli B,Ciurli S

更新日期：2006-05-23 00:00:00

abstract：:For the study of the protein--protein and protein--nucleic acid interactions in the assembly of virus particles, laser Raman spectra have been obtained in H2O and D2O solutions and as a function of temperature for the following Salmonella phage P22 components: mature phage particles, isolated mature phage DNA, mature ...

journal_title：Biochemistry

authors： Thomas GJ Jr,Li Y,Fuller MT,King J

更新日期：1982-08-03 00:00:00