Effects of pH and calcium ions on the conformational transitions in silk fibroin using 2D Raman correlation spectroscopy and 13C solid-state NMR.

abstract：:The thermal denaturation of the cupredoxin plastocyanin (PC) from spinach has been studied with the aim of improving the understanding of factors involved in the conformational stability of antiparallel beta-sheet proteins. Studies using differential scanning calorimetry have been complemented with nuclear magnetic re...

journal_title：Biochemistry

authors： Sandberg A,Harrison DJ,Karlsson BG

更新日期：2003-09-02 00:00:00

abstract：:A human cDNA, UDPGTh-3, encoding a dihydrotestosterone/5 alpha-androstane-3 alpha,17 beta-diol UDP- glucuronosyltransferase (transferase) has been isolated and characterized. The nucleotide sequence of UDPGTh-3 encodes a 530 amino acid protein with a typical membrane insertion-signal peptide, a membrane-anchoring doma...

journal_title：Biochemistry

authors： Chen F,Ritter JK,Wang MG,McBride OW,Lubet RA,Owens IS

更新日期：1993-10-12 00:00:00

abstract：:Succinyl-CoA synthetase of Escherichia coli is an alpha 2 beta 2 protein containing active sites at the interfaces between alpha- and beta-subunits. The alpha-subunit contains a histidine residue that is phosphorylated during the reaction. The beta-subunit binds coenzyme A and probably succinate [see Nishimura, J. S. ...

journal_title：Biochemistry

authors： Ybarra J,Prasad AR,Nishimura JS

更新日期：1986-11-04 00:00:00

abstract：:TcmO is an O-methyltransferase that methylates the C-8 hydroxyl to Tcm B3, a four-ring aromatic intermediate in the tetracenomycin biosynthetic pathway of Streptomyces glaucescens. The gene encoding this enzyme was expressed in Streptomyces coelicolor CH999 together with the actinorhodin polyketide synthase (PKS) gene...

journal_title：Biochemistry

authors： Fu H,Alvarez MA,Khosla C,Bailey JE

更新日期：1996-05-28 00:00:00

abstract：:The manganese transport regulator (MntR) of Bacillus subtilis is activated by Mn(2+) to repress transcription of genes encoding transporters involved in the uptake of manganese. MntR is also strongly activated by cadmium, both in vivo and in vitro, but it is poorly activated by other metal cations, including calcium a...

journal_title：Biochemistry

authors： Kliegman JI,Griner SL,Helmann JD,Brennan RG,Glasfeld A

更新日期：2006-03-21 00:00:00

abstract：:The bacterial phosphotriesterase (PTE) from Pseudomonas diminuta catalyzes the hydrolysis of organophosphate esters at rates close to the diffusion limit. X-ray diffraction studies have shown that a binuclear metal center is positioned in the active site of PTE and that this complex is responsible for the activation o...

journal_title：Biochemistry

authors： Kim J,Tsai PC,Chen SL,Himo F,Almo SC,Raushel FM

更新日期：2008-09-09 00:00:00

abstract：:The interaction of aminooxy compounds such as aminooxyacetate (AAA), L-canaline, and hydroxylamine with sheep liver serine hydroxymethyltransferase (EC 2.1.2.1) was studied by absorption spectra and stopped-flow spectrophotometry and compared with the unique feature of interaction of O-amino-D-serine (OADS) with the e...

journal_title：Biochemistry

authors： Baskaran N,Prakash V,Savithri HS,Radhakrishnan AN,Appaji Rao N

更新日期：1989-12-12 00:00:00

abstract：:The importance of the evolutionarily conserved Gly-4 residue for the affinity and kinetics of interaction of cystatin A with several cysteine proteinases was assessed by site-directed mutagenesis. Even the smallest replacement, by Ala, resulted in approximately 1000-, approximately 10- and approximately 6000-fold decr...

journal_title：Biochemistry

authors： Estrada S,Nycander M,Hill NJ,Craven CJ,Waltho JP,Björk I

更新日期：1998-05-19 00:00:00

abstract：:Aldehyde dehydrogenase 7A1 (ALDH7A1) is part of lysine catabolism and catalyzes the NAD(+)-dependent oxidation of α-aminoadipate semialdehyde to α-aminoadipate. Herein, we describe a structural study of human ALDH7A1 focused on substrate recognition. Five crystal structures and small-angle X-ray scattering data are re...

journal_title：Biochemistry

authors： Luo M,Tanner JJ

更新日期：2015-09-08 00:00:00

abstract：:The structures of the modified folates present in Pyrococcus furiosus have been determined. This was accomplished largely by the characterization of the arylamines resulting from the air oxidative cleavage of the reduced modified folates present in these cells, using both chemical and enzymatic methods. Cell extracts ...

journal_title：Biochemistry

authors： White RH

更新日期：1993-01-26 00:00:00

abstract：:The catalytic activity of protein-tyrosine phosphatases (PTPs) is mediated by a cysteine side chain which carries out a nucleophilic attack initiating the phosphate cleavage. Experimentally, it has been observed that the active site cysteine has a remarkably low pKa. In the present study, we have investigated the orig...

journal_title：Biochemistry

authors： Peters GH,Frimurer TM,Olsen OH

更新日期：1998-04-21 00:00:00

abstract：:To gain insight into the physiological function of phosphoinositide 3-kinase (PI 3-kinase) lipid products, this study examines the interactions of the D-3 phosphoinositides with profilin and the consequent effects on actin dynamics and phosphoinositide turnover. Profilin, a ubiquitous actin-regulating protein, plays a...

journal_title：Biochemistry

authors： Lu PJ,Shieh WR,Rhee SG,Yin HL,Chen CS

更新日期：1996-11-05 00:00:00

abstract：:Temporins are short (10-13 amino acids) and linear antimicrobial peptides first isolated from the skin of the European red frog, Rana temporaria, and are effective against Gram-positive bacteria and Candida albicans. To get insight into their mechanism(s) of action, we compared the effects on model membranes exerted b...

journal_title：Biochemistry

authors： Zhao H,Rinaldi AC,Di Giulio A,Simmaco M,Kinnunen PK

更新日期：2002-04-02 00:00:00

abstract：:The flexibility and curvature of duplex DNAs containing mismatched sites have been monitored as a function of temperature. The diffusion coefficients are dependent on the flexibility and the curvature of the DNA, and these have been determined by NMR-based methods. The diffusion coefficients, D, depend on a Boltzmann ...

journal_title：Biochemistry

authors： Marathias VM,Jerkovic B,Arthanari H,Bolton PH

更新日期：2000-01-11 00:00:00

abstract：:Using the thrombin-cut [68-30 kilodalton (kDa)] myosin subfragment 1 (S-1) whose heavy chain has been selectively split within the central 50-kDa region, at Lys-560, with concomitant specific alterations of the ATPase and actin binding properties [Chaussepied, P., Mornet, D., Audemard, E., Derancourt, J., & Kassab, R....

journal_title：Biochemistry

authors： Chaussepied P,Mornet D,Kassab R

更新日期：1986-10-21 00:00:00

abstract：:Inhibitors of poly(ADP-ribose) polymerase (PARP, EC 2.4.2.30) are of clinical interest because they have potential for improving radiation therapy and chemotherapy of cancer. The refined binding structures of four such inhibitors are reported together with the refined structure of the unligated catalytic fragment of t...

journal_title：Biochemistry

authors： Ruf A,de Murcia G,Schulz GE

更新日期：1998-03-17 00:00:00

abstract：:Changes in the concentrations of high-energy phosphate metabolites were measured by 31P NMR spectroscopy of surviving rat uteri from 0-48 h following estrogen administration. Concentrations (millimoles per kilogram wet weight) of these metabolites in the untreated immature uterus, measured at 4 degrees C, were found t...

journal_title：Biochemistry

authors： Degani H,Shaer A,Victor TA,Kaye AM

更新日期：1984-06-05 00:00:00

abstract：:The operations within a living cell depend on the collective activity of networks of proteins, sometimes termed "interactomes". Within these networks, most proteins interact with few partners, while a small proportion of proteins, called hubs, participate in a large number of interactions and play a central role in or...

journal_title：Biochemistry

authors： Barbar E

更新日期：2008-01-15 00:00:00

abstract：:The mutagenic and genotoxic properties of 1,N6-ethenoadenine (epsilon Ade), 3,N4-ethenocytosine (epsilon Cyt), and 4-amino-5-(imidazol-2-yl)imidazole (beta) were investigated in vivo. The former two modified bases are known DNA adducts formed by the human carcinogen vinyl chloride; beta is formed by pyrimidine ring-op...

journal_title：Biochemistry

authors： Basu AK,Wood ML,Niedernhofer LJ,Ramos LA,Essigmann JM

更新日期：1993-11-30 00:00:00

abstract：:Aminoglycoside 2''-phosphotransferase IVa [APH(2'')-IVa] is a member of a family of bacterial enzymes responsible for medically relevant resistance to antibiotics. APH(2'')-IVa confers high-level resistance against several clinically used aminoglycoside antibiotics in various pathogenic Enterococcus species by phospho...

journal_title：Biochemistry

authors： Shi K,Houston DR,Berghuis AM

更新日期：2011-07-19 00:00:00

abstract：:Brief exposure of purple membrane (PM) to dilute Triton X-100 eliminates the actinic light effect on the relative amounts of fast M (Mf) and slow M (Ms) intermediates and alters the character and kinetics of the photocycle, without destroying the native BR trimers (Mukhopadhyay et al., 1994). Particular membrane lipid...

journal_title：Biochemistry

authors： Mukhopadhyay AK,Dracheva S,Bose S,Hendler RW

更新日期：1996-07-16 00:00:00

abstract：:Lactyl-CoA dehydratase consists of two enzymes, E1 and E2, and requires catalytic quantities of ATP for activity [Kuchta, R. D., & Abeles, R. H. (1985) J. Biol. Chem. 260, 13181-13189]. In contrast to E1, which contains no Fe, E2 contains 8.20 +/- 0.04 mol of Fe/mol of E2, one of which can be removed by 1,10-phenanthr...

journal_title：Biochemistry

authors： Kuchta RD,Hanson GR,Holmquist B,Abeles RH

更新日期：1986-11-18 00:00:00

abstract：:Insulin decreases multifunctional protein kinase (MFPK) activity in rat adipose tissue [Ramakrishna, S., & Benjamin, W. B. (1988) J. Biol. Chem. 263, 12677-12681]. Insulin also decreases the phosphorylation of peptide B but increases the phosphorylation of peptide A of ATP-citrate lyase (ATP-CL). The mechanism for thi...

journal_title：Biochemistry

authors： Ramakrishna S,Murthy KS,Benjamin WB

更新日期：1989-01-24 00:00:00

abstract：:The photochemical and subsequent thermal reactions of the mouse short-wavelength visual pigment (MUV) were studied by using cryogenic UV-visible and FTIR difference spectroscopy. Upon illumination at 75 K, MUV forms a batho intermediate (lambda(max) approximately 380 nm). The batho intermediate thermally decays to the...

journal_title：Biochemistry

authors： Dukkipati A,Kusnetzow A,Babu KR,Ramos L,Singh D,Knox BE,Birge RR

更新日期：2002-08-06 00:00:00

abstract：:The linker for activation of T-cells (LAT) is a palmitoylated integral membrane adaptor protein that resides in lipid membrane rafts and contains nine consensus putative tyrosine phosphorylation sites, several of which have been shown to serve as SH2 binding sites. Upon T-cell antigen receptor (TCR/CD3) engagement, LA...

journal_title：Biochemistry

authors： Perez-Villar JJ,Whitney GS,Sitnick MT,Dunn RJ,Venkatesan S,O'Day K,Schieven GL,Lin TA,Kanner SB

更新日期：2002-08-27 00:00:00

abstract：:The occupancy of the central cavity of the membrane-embedded c ring of the ATP synthase of Escherichia coli was investigated with a photo-cross-linking approach. Single cysteine mutants were created at c subunit positions 4, 8, and 11, which are oriented to the inside of the ring. These cysteines were alkylated with r...

journal_title：Biochemistry

authors： Oberfeld B,Brunner J,Dimroth P

更新日期：2006-02-14 00:00:00

abstract：:We examined the interactions of nucleotides with the CMP-sialic acid transporter in order to better understand which features play a role in binding and to investigate the relationship between binding and subsequent transport. With respect to the sugar, the transporter requires a complete ribose ring for tight binding...

journal_title：Biochemistry

authors： Chiaramonte M,Koviach JL,Moore C,Iyer VV,Wagner CR,Halcomb RL,Miller W,Melançon P,Kuchta RD

更新日期：2001-11-27 00:00:00

abstract：:The 19-transmembrane multisubunit γ-secretase complex generates the amyloid β-peptide (Aβ) of Alzheimer's disease (AD) by intramembrane proteolysis of the β-amyloid precursor protein (APP). Despite substantial advances in elucidating how this protein complex functions, the effect of the local membrane lipid microenvir...

journal_title：Biochemistry

authors： Holmes O,Paturi S,Ye W,Wolfe MS,Selkoe DJ

更新日期：2012-05-01 00:00:00

abstract：:Hybridization experiments with variants of an oligomeric protein often provide important information regarding subunit structure, function, and interactions. In some systems, however, the variants are so similar electrophoretically and chromatographically that purification of individual hybrids is not feasible. Theref...

journal_title：Biochemistry

authors： Gibbons I,Schachman HK

更新日期：1976-01-13 00:00:00

abstract：:Beta-amylase (EC 3.2.1.2) is starch-hydrolyzing exo-type enzyme that can catalyze the successive liberation of beta-maltose from the nonreducing ends of alpha-1,4-linked glucopyranosyl polymers. There is a well-known phenomenon called multiple or repetitive attack where the enzyme releases several maltose molecules in...

journal_title：Biochemistry

authors： Ishikawa K,Nakatani H,Katsuya Y,Fukazawa C

更新日期：2007-01-23 00:00:00