Abstract:
:We examined the interactions of nucleotides with the CMP-sialic acid transporter in order to better understand which features play a role in binding and to investigate the relationship between binding and subsequent transport. With respect to the sugar, the transporter requires a complete ribose ring for tight binding, and the 2'-ara hydrogen makes an important contact. The enzyme exhibits little specificity with respect to the 2'- and 3'-hydroxyls, as it tolerated substitutions ranging from fluorine to an azido group. In the base, the C4 amine and C2 carbonyl groups make important contacts, while the N3 nitrogen does not. However, adding a methyl group to N3 dramatically reduced binding, indicating that mass at this position sterically hinders binding. Adding a group at C5 had either no effect or slightly enhanced binding. To determine if the transporter recognizes these CMP analogues as substrates, we assayed them for their ability to trans stimulate CMP-sialic acid import. These data suggest that the enzyme transports a wide variety of NMPs, and the rate of transport is inversely proportional to the K(I) of the analogue. The importance of our findings for understanding the specificities of the different nucleotide-sugar tranlocators and the design of novel glycosylation inhibitors are discussed.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Chiaramonte M,Koviach JL,Moore C,Iyer VV,Wagner CR,Halcomb RL,Miller W,Melançon P,Kuchta RDdoi
10.1021/bi011262wsubject
Has Abstractpub_date
2001-11-27 00:00:00pages
14260-7issue
47eissn
0006-2960issn
1520-4995pii
bi011262wjournal_volume
40pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract::Cytochrome P450 BM-3 is a bacterial enzyme with sequence similarity to mammalian P450s that catalyzes the hydroxylation of fatty acids with high efficiency. Enzyme-substrate binding and dynamics has been an important topic of study for cytochromes P450 because most of the crystal structures of substrate-bound structur...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi4000645
更新日期:2013-10-01 00:00:00
abstract::The chromatin-associated high-mobility group (HMG) proteins of the plant HMGB family are characterized by a central HMG-box domain that is flanked by a basic N-terminal and an acidic C-terminal domain. By functional interaction with certain transcription factors, HMGB proteins contribute to transcriptional regulation....
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi6024947
更新日期:2007-05-29 00:00:00
abstract::The synthesis is described of four new cleavable, photosensitive, heterobifunctional cross-linking reagents for use in examining membrane organization: 4'-azidoazobenzene-4oxysuccinimide ester (1), N-[4-(p-azidophenylazo)benzoyl]-3-aminopropyl-N'-oxysuccinimide ester (2), N-[4-(p-azidophenylazo)benzoyl]-6-aminohexyl-N...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00560a007
更新日期:1980-09-16 00:00:00
abstract::The crystal structure of fiddler crab collagenase complexed with the dimeric serine protease inhibitor ecotin at 2.5 A resolution reveals an extended cleft providing binding sites for at least 11 contiguous substrate residues. Comparison of the positions of nine intermolecular main chain hydrogen bonding interactions ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9617522
更新日期:1997-05-06 00:00:00
abstract::F-STOP is a microtubule-associated protein that stabilizes microtubules in a calmodulin (CaM)-dependent manner. All members of the stable tubule only polypeptide (STOP) family have a central domain that contains nearly identical multiple repeats, and a CaM binding motif is present in multiple copies within this domain...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi034746w
更新日期:2003-10-07 00:00:00
abstract::Investigation of the charge distribution for all known members of the PP-fold family of peptides reveals a common pattern characterized by a cluster of negative charges in the beta-turn region and a cluster of positive charges in the receptor-binding region of the peptide. Detailed analysis of the electrostatic proper...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00063a005
更新日期:1993-03-30 00:00:00
abstract::The human transferrin receptor (TfR) has three N-linked oligosaccharides. A combination of site-directed mutagenesis and carbohydrate and protein chemistry was used to characterize the structures of the N-linked oligosaccharides and to map their locations. We find that the type of oligosaccharide at each position was ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi962884y
更新日期:1997-04-29 00:00:00
abstract::Isoforms of transforming growth factor beta (TGFbeta) are 25 kDa homodimeric polypeptides that signal by binding and bringing together two related, functionally distinct cell surface receptors designated as TbetaR1 and TbetaR2. Here, we report the solution structure of the 13.8 kDa extracellular domain of human TbetaR...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi034366a
更新日期:2003-09-02 00:00:00
abstract::His216 is a well-conserved residue in pyruvate carboxylases and, on the basis of structures of the enzyme, appears to have a role in the binding of MgATP, forming an interaction with the 3'-hydroxyl group of the ribose ring. Mutation of this residue to asparagine results in a 9-fold increase in the Km for MgATP in its...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi4016814
更新日期:2014-02-18 00:00:00
abstract::Sulfonylureas and glinides are commonly used oral insulin secretagogues (ISs) that act on the pancreatic ATP-sensitive potassium (KATP) channel to promote insulin secretion in order to lower the blood glucose level. Physiologically, KATP channels are inhibited by intracellular ATP and activated by Mg-ADP. Therefore, t...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.9b00692
更新日期:2020-01-14 00:00:00
abstract::Extracellular senile plaques composed predominantly of fibrillar amyloid-beta (Abeta) are a major neuropathological feature of Alzheimer's disease (AD). Genetic evidence and in vivo studies suggest that apolipoprotein E (apoE) may contribute to amyloid clearance and/or deposition. In vitro studies demonstrate that nat...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0017475
更新日期:2000-12-26 00:00:00
abstract::The isolated N-terminal Src homology 3 (SH3) domain of Drosophila drk exists in equilibrium between folded and unfolded states in aqueous buffer near neutral pH. Nuclear magnetic resonance spectra recorded on both states simultaneously exhibit an approximate 1:1 ratio of protein conformations. The folded form is simil...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00020a025
更新日期:1995-05-23 00:00:00
abstract::Semisynthetic aminoglycoside derivatives may provide a means to selectively target viral RNA sites, including the HIV-1 Rev response element (RRE). The design, synthesis, and evaluation of derivatives based upon neomycin B, kanamycin A, and tobramycin conjugates of 9-aminoacridine are presented. To evaluate the import...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi034766y
更新日期:2003-10-07 00:00:00
abstract::The allosteric interactions that regulate substrate channeling and catalysis in the tryptophan synthase bienzyme complex from Salmonella typhimurium are triggered by covalent reactions at the beta-site and binding of substrate/product to the alpha-site. The transmission of these allosteric signals between the alpha- a...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi034291a
更新日期:2003-07-01 00:00:00
abstract::Detection of specific reaction products is a powerful approach for dissecting out pathways that mediate oxidative damage in vivo. Eosinophil peroxidase (EPO), an abundant protein secreted from activated eosinophils, has been implicated in promoting oxidative tissue injury in conditions such as asthma, allergic inflamm...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi982401l
更新日期:1999-03-23 00:00:00
abstract::A debilitating complication of long-term hemodialysis is the deposition of beta-2-microglobulin (beta2m) as amyloid plaques in the joint space. We have recently shown that Cu(2+) can be a contributing, if not causal, factor at concentrations encountered during dialysis therapy. The basis for this effect is destabiliza...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi025944a
更新日期:2002-08-27 00:00:00
abstract::The mitogen-activated protein kinases (MAPKs) are key components of cellular signal transduction pathways, which are down-regulated by the MAPK phosphatases (MKPs). Catalytic activity of the MKPs is controlled both by their ability to recognize selective MAPKs and by allosteric activation upon binding to MAPK substrat...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.7b00827
更新日期:2017-11-21 00:00:00
abstract::The two most basic charge isomers of myelin basic protein (BP), components 1 and 2 (C1 and C2), which presumably differ in the degree of deamidation, were purified from bovine BP by cation-exchange chromatography. Two additional specific types of posttranslational modifications were introduced into the purified isomer...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00340a032
更新日期:1985-09-10 00:00:00
abstract::The proprotein convertase furin is a highly specific serine protease modifying and thereby activating proteins in the secretory pathway by proteolytic cleavage. Its substrates are involved in many diseases, including cancer and infections caused by bacteria and viruses. Understanding furin's substrate specificity is c...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.7b01124
更新日期:2018-02-13 00:00:00
abstract::Variations for non-histones in the ADP-ribosylating activities of interphase and metaphase cells were investigated. 32P-Labeled nicotinamide adenine dinucleotide ([32P]NAD), the specific precursor for the modification, was used to radioactively label proteins. Permeabilized interphase and mitotic cells, as well as iso...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00323a017
更新日期:1985-01-15 00:00:00
abstract::We have examined the structure of newly repaired regions of chromatin in intact and permeabilized human cells following exposure to bleomycin (BLM). The average repair patch size (in permeabilized cells) was six to nine bases, following doses of 1-25 micrograms/mL BLM, and greater than 80% of the total repair synthesi...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00484a020
更新日期:1990-08-14 00:00:00
abstract::The structure and function of iron(II)-ruthenium(II) hybrid hemoglobins alpha(Ru-CO)2 beta(Fe)2 and alpha(Fe)2 beta(Ru-CO)2, which can serve as models for the intermediate species of the oxygenation step in native human adult hemoglobin, were investigated by measuring oxygen equilibrium curves and the Fe(II)-N epsilon...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00447a050
更新日期:1989-10-17 00:00:00
abstract::Neuroglobin (Ngb) is a recently discovered vertebrate heme protein that is expressed in the brain and can reversibly bind oxygen. Mammalian Ngb is involved in neuroprotection under oxidative stress conditions, such as ischemia and reperfusion. We previously demonstrated that human ferric Ngb binds to the alpha subunit...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi800286m
更新日期:2008-05-13 00:00:00
abstract::The acetylenic analogue of adenosine 9-(5',6'-dideoxy-beta-D-ribo-hex-5'-ynofuranosyl)adenine has been synthesized, and its behavior as an inhibitor of bovine S-adenosylhomocysteine hydrolase has been examined. Incubation of the enzyme with excess inhibitor caused a time-dependent, irreversible inactivation of the enz...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00105a025
更新日期:1991-10-15 00:00:00
abstract::The unique structural properties of the ferritin protein cages have provided impetus to focus on the methodical study of these self-assembling nanosystems. Among these proteins, Escherichia coli bacterioferritin (EcBfr), although architecturally very similar to other members of the family, shows structural instability...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi200207w
更新日期:2011-05-17 00:00:00
abstract::Transport of hydrophilic di- and tripeptides into Lactococcus lactis is mediated by a proton motive force-driven peptide transport protein (DtpT) that shares similarity with eukaryotic peptide transporters, e.g., from kidney and small intestine of rabbit, man, and rat. Hydropathy profiling in combination with the "pos...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi963068t
更新日期:1997-06-03 00:00:00
abstract::Few techniques can identify interactions between proteins and individual water molecules when the protein is in solution. The present work has sought to bridge the gap between the molecular level studies and the search for a physical property of the solution (bathing the proteins) that would regulate the protein hydra...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi012210+
更新日期:2002-08-13 00:00:00
abstract::We have studied the transient kinetics of electron transfer from a positively charged viologen analogue (propylene diquat), reduced by pulsed laser excitation of the deazariboflavin/EDTA system, to the net negatively charged ferredoxins from spinach and Clostridium pasteurianum. Spinach ferredoxin showed monophasic ki...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00440a050
更新日期:1989-07-11 00:00:00
abstract::Isothermal titration calorimetry (ITC) is a powerful technique for investigating self-association processes of protein complexes and was expected to reveal quantitative data on peroxiredoxin oligomerization by directly measuring the thermodynamic parameters of dimer-dimer interaction. Recombinant classical 2-cysteine ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi8002956
更新日期:2008-07-08 00:00:00
abstract::A new fluorescence method to measure viscosity in cell cytosol [Fushimi, K., & Verkman, A. S. (1991) J. Cell Biol. 112, 719-725] has been applied to determine fluid-phase viscosity in sea urchin eggs. Freshly harvested eggs from Lytechinus pictus were loaded with the dyes 2,7-bis(2-carboxyethyl)-5-(and-6-)carboxyfluor...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00115a600
更新日期:1991-12-24 00:00:00