Abstract：
:The interaction of aminooxy compounds such as aminooxyacetate (AAA), L-canaline, and hydroxylamine with sheep liver serine hydroxymethyltransferase (EC 2.1.2.1) was studied by absorption spectra and stopped-flow spectrophotometry and compared with the unique feature of interaction of O-amino-D-serine (OADS) with the enzyme [Baskaran, N., Prakash, V., Appu Rao, A. G., Radhakrishnan, A. N., Savithri, H. S., & Appaji Rao, N. (1989) Biochemistry (preceding paper in this issue)]. The reaction of AAA (0.5 mM) with the Schiff base of the enzyme resulted in the formation of pyridoxal 5'-phosphate (PLP) and was biphasic with rate constants of 191 and 19 s-1. The formation of the PLP-AAA oxime measured by decrease in absorbance at 388 nm on interaction of AAA with the enzyme had a rate constant of 5.2 M-1 s-1. On the other hand, the reaction of L-canaline with the enzyme was slower as measured by the disruption of enzyme-Schiff base than the reaction of OADS and AAA. In contrast, the formation of PLP as an intermediate could not be detected upon the interaction of hydroxylamine with the enzyme. The reaction of D-cycloserine with the enzyme was much slower (1.6 x 10(2) M-1 s-1) than the aminooxy compounds. These observations indicate that the aminooxy compounds that are structural analogues of serine (OADS, AAA, and canaline) formed PLP as an intermediate prior to the formation of oxime, whereas with hydroxylamine such an intermediate could not be detected.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Baskaran N,Prakash V,Savithri HS,Radhakrishnan AN,Appaji Rao Ndoi
10.1021/bi00451a010subject
Has Abstractpub_date
1989-12-12 00:00:00pages
9613-7issue
25eissn
0006-2960issn
1520-4995journal_volume
28pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract：:A conserved, crystallographically defined bile acid binding site was originally identified in the membrane domain of mammalian and bacterial cytochrome c oxidase (CcO). Current studies show other amphipathic molecules including detergents, fatty acids, steroids, and porphyrins bind to this site and affect the already ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi3014505
更新日期：2013-02-26 00:00:00
abstract：:The effects of ions on the interaction of lac repressor protein and operator DNA have been studied by the membrane filter technique. The equilibrium association constant was determined as a function of monovalent and divalent cation concentrations, anions, and pH. The binding of repressor and operator is extremely sen...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00516a027
更新日期：1981-06-23 00:00:00
abstract：:Endonuclease V of bacteriophage T4 binds to UV-irradiated deoxyribonucleic acid (DNA) but not to unirradiated DNA. We have developed an assay to detect this binding, based on the retention of enzyme--DNA complexes on nitrocellulose filters. The amount of complex retained, ascertained by using radioactive DNA, is a mea...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00549a026
更新日期：1980-04-15 00:00:00
abstract：:Thymidylate synthetase catalyzes an exchange of tritium of [5-3H]dUMP for protons of water in the absence of CH2-H4folate. The turnover number for this reaction is some 45,000-fold lower than that of dTMP formation and Km is 1.2 X 10(-5) M, similar to the dissociation constant of the enzyme-dUMP complex determined by ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00580a016
更新日期：1979-06-26 00:00:00
abstract：:The p53 protein family is involved in the control of an intricate network of genes implicated in cell cycle, through to germ line integrity and development. Although the role of p53 is well-established, the intrinsic nature of its homologue p73 has yet to be fully elucidated. Here, the biochemical characterization and...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi7023207
更新日期：2008-03-11 00:00:00
abstract：:Escherichia coli helicase II (UvrD) protein can initiate unwinding of duplex DNA at blunt ends or nicks, although these reactions require excess protein. We have undertaken kinetic studies of these reactions in order to probe the mechanism of initiation of unwinding. DNA unwinding was monitored directly by using agaro...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00066a039
更新日期：1993-04-20 00:00:00
abstract：:To explore the interactions of triacylglycerol and phospholipid hydrolysis in lipoprotein conversions and remodeling, we compared the activities of lipoprotein and hepatic lipases on human VLDL, IDL, LDL, and HDL2. Triacylglycerol and phospholipid hydrolysis by each enzyme were measured concomitantly in each lipoprote...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00151a023
更新日期：1992-09-15 00:00:00
abstract：:The chromatin-associated high-mobility group (HMG) proteins of the plant HMGB family are characterized by a central HMG-box domain that is flanked by a basic N-terminal and an acidic C-terminal domain. By functional interaction with certain transcription factors, HMGB proteins contribute to transcriptional regulation....
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi6024947
更新日期：2007-05-29 00:00:00
abstract：:Each regulatory subunit of the cAMP-dependent protein kinase contains two in-tandem cAMP binding sites. Photolabeling of holoenzyme I with 8-azidoadenosine 3',5'-monophosphate (8-N3-cAMP) leads to the covalent modification of two residues, Trp-260 and Tyr-371. In order to correlate photolabeling of these two residues ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00386a035
更新日期：1987-06-16 00:00:00
abstract：:To probe the mechanism of the reversible DNA phosphodiester bond cleavage and religation mechanism of the type I topoisomerase from vaccinia virus, we have synthesized DNA substrates carrying a single nonbridging Rp- or Sp-phosphorothioate (Ps) modification at the scissile phosphodiester (Pd) bond. Analysis of the ste...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi992429c
更新日期：2000-05-09 00:00:00
abstract：:The role of ATP hydrolysis by the 44/62 protein in formation of the stable holoenzyme DNA replication complex has been further elucidated by specifically examining the role that the 44/62 protein plays in loading the 45 protein onto the DNA substrate. A stable phospho-45 protein or phosphorylated holoenzyme complex wa...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi962139l
更新日期：1997-03-11 00:00:00
abstract：:Malondialdehyde (MDA) and nucleobase propenals can transfer oxopropenyl groups to guanine residues of DNA to yield pyrimodopurinone (M(1)G) adducts. The DNA structural requirements for reaction with alpha,beta-unsaturated aldehydes were explored. We found that single-stranded DNA is more sensitive to oxopropenylation ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0113059
更新日期：2002-04-16 00:00:00
abstract：:The crystal structure of human platelet factor 4 (PF4) has been solved to a resolution of 2.4 A by molecular replacement and refined to an R-factor of 24.1%. The structure consists of four polypeptide chains which form a tetrameric unit. N-terminal residues, previously defined as a random coil or extended loop region,...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00193a025
更新日期：1994-07-12 00:00:00
abstract：:Porcine pancreatic phospholipase A2 interacts with micelles of the substrate analogue n-octadecylphosphocholine to form a specific complex over considerably wide concentration ranges of both lipid and protein. UV absorption difference spectroscopy measurements indicate that the ratio of lipid to protein molecules in t...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00517a019
更新日期：1981-07-07 00:00:00
abstract：:The regiospecificity and stereospecificity of proton transfer in the yeast inorganic pyrophosphatase (PPase) catalyzed hydrolysis of P1,P2-bidentate Mg(H2O)4(PPi)2- were probed with exchange-inert metal complexes of imidodiphosphate (PNP) and thiopyrophosphate (PPS). PPase was unable to catalyze the hydrolysis of Mg(H...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00364a035
更新日期：1986-08-12 00:00:00
abstract：:The potassium form of d(CGCGAATTCGCG) solved by X-ray diffraction to 1.75 A resolution indicates that monovalent cations penetrate the primary and secondary layers of the "spine of hydration". Both the sodium [Shui, X., McFail-Isom, L., Hu, G. G., and Williams, L. D. (1998) Biochemistry 37, 8341-8355] and the potassiu...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi982063o
更新日期：1998-12-01 00:00:00
abstract：:Thermal and chemical modification of protein structures is known to affect their interfacial activity. We have looked in detail at the effect of heating on the structure and subsequently the surface properties of LTP1, a nonspecific lipid transfer protein from barley, both in the presence and in the absence of its lip...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi901328f
更新日期：2009-12-29 00:00:00
abstract：:Endonuclease VIII (Nei) is one of three enzymes in Escherichia coli that are involved in base-excision repair of oxidative damage to DNA. We investigated the substrate specificity and excision kinetics of this DNA glycosylase for bases in DNA that have been damaged by free radicals. Two different DNA substrates were p...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi015552o
更新日期：2001-10-09 00:00:00
abstract：:Amyloid beta peptide (A beta) is released into the media of a variety of cells in culture during normal metabolism. The discovery of several missense mutations within or flanking the A beta region of the beta amyloid precursor protein (beta APP) in familial Alzheimer's disease provides strong evidence for a role of al...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00181a016
更新日期：1994-04-19 00:00:00
abstract：:In an attempt to understand the mechanism by which transcription factors compete with histone octamers for cognate binding sites in chromatin, the effect of the histone binding protein nucleoplasmin on the binding of TFIIIA to nucleosomal 5S rRNA genes was tested. In this study, it was shown that, despite the previous...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi972723+
更新日期：1998-02-03 00:00:00
abstract：:The virally encoded integrase protein carries out retroviral integration, which requires specific interactions with the two ends of the viral DNA, and also with host DNA that is the target of integration. We attached a photo-cross-linking agent to specific viral and target DNA sites to identify regions of the integras...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi972949c
更新日期：1998-05-12 00:00:00
abstract：:One of the most promising approaches to anti-hepatitis C virus drug discovery is the development of inhibitors of the virally encoded protease NS3. This chymotrypsin-like serine protease is essential for the maturation of the viral polyprotein, and processing requires complex formation between NS3 and its cofactor NS4...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi991220w
更新日期：1999-10-19 00:00:00
abstract：:αB-Crystallin is a chaperone and an anti-apoptotic protein that is strongly expressed in many tissues, including the lens, retina, heart, and kidney. In the human lens, several lysine residues in αB-crystallin are acetylated. We have previously shown that such acetylation is predominant at lysine 92 (K92) and lysine 1...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi400638s
更新日期：2013-11-12 00:00:00
abstract：:All known Mn-containing superoxide dismutases (MnSODs) have a highly conserved histidine (His-30 in Escherichia coli FeSOD) in the active-site channel, and nearly all have an active-site arginine (Arg-170) that has been proposed to play a combined structural and functional role [Chan et al., Arch. Biochem. Biophys. 27...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi980167t
更新日期：1998-08-11 00:00:00
abstract：:Horseradish peroxidase (HRP) was oxidized by IrCl6(2-) to a mixture of compounds I and II, the rate of oxidation and the ratio of the mixture being greatly affected by pH (Hayashi & Yamazaki, 1979). Oxidation of HRP by IrCl6(2-) in the presence of fluoride was significantly accelerated. This resulted in the formation ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00046a003
更新日期：1995-11-21 00:00:00
abstract：:To elucidate early stages in protein folding, we have adopted a fragment reconstitution method for small proteins. This approach is expected to provide nuclei for protein folding and to allow us to investigate folding mechanisms. In previous work [Kobayashi, N., et al. (1995) FEBS Lett. 366, 99-103.] we demonstrated t...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi982271g
更新日期：1999-01-26 00:00:00
abstract：:We have purified and characterized a novel 30-kDa glycoprotein (gp30) with TGF alpha-like properties secreted from the estrogen receptor negative breast cancer cell line MDA-MB-231. This factor was immunoprecipitated by an anti-TGF alpha polyclonal antibody and also had TGF alpha-like biological activity, as assayed b...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00147a018
更新日期：1992-08-18 00:00:00
abstract：:To identify how many rhodopsin intermediates interact with retinal G-protein transducin, the photobleaching process of chicken rhodopsin has been investigated in the presence or absence of transducin by means of time-resolved low-temperature spectroscopy. Singular value decomposition (SVD) analysis of the spectral dat...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi970932o
更新日期：1997-11-18 00:00:00
abstract：:The di-heme enzyme MauG catalyzes the oxidative biosynthesis of a tryptophan tryptophylquinone cofactor on a precursor of the enzyme methylamine dehydrogenase (preMADH). Reaction of H2O2 with the diferric form of MauG, or reaction of O2 with diferrous MauG, forms the catalytic intermediate known as bis-Fe(IV), which a...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi401441h
更新日期：2013-12-31 00:00:00
abstract：:The effects of several divalent cations, various polybasic amines, and lysozyme on the rate of polymerization of 8S clathrin to the 300S coat structure have been evaluated by turbidimetric procedures. Ca2+ and Mn2+ strongly enhance the rate of polymerization. Only spermine among the naturally occurring polybasic amine...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00526a028
更新日期：1981-11-10 00:00:00
