Abstract:
:Peripherin/rds is a 39 kDa integral membrane glycoprotein essential for normal photoreceptor cell development in vertebrates. It has been implicated in several human retinal degenerative diseases including retinitis pigmentosa and macular degeneration and is thought to play a structural role at photoreceptor outer segment disk rims, where it forms a tightly-associated complex with rom-1, a nonglycosylated 37 kDa homologue. Western blot analysis of COS-1 cells transiently transfected with full-length cDNA coding for either peripherin/rds or rom-1 indicates that each protein is expressed primarily as a disulfide-linked homodimer; recombinant peripherin/rds is glycosylated while recombinant rom-1 is not--akin to their counterparts in rod photoreceptor disk membranes. Upon cotransfection of the two cDNAs, the specific assembly of a stable peripherin/rds--rom-1 complex is observed. Immunofluorescence microscopy studies demonstrate that both singly and coexpressed peripherin/rds and rom-1 complexes are localized primarily within internal membranes of transfected cells. Velocity sedimentation data indicate that the recombinant complexes (4.9 S) are assembled with a subunit stoichiometry similar to those extracted from ROS membranes (4.5 S) and are most consistent with a tetrameric arrangement of polypeptides. Sedimentation analyses of individually expressed peripherin/rds (5.1 S) and rom-1 (4.3 S) suggest that each polypeptide can also assemble into a tetrameric form in the absence of its homologue partner. Subunit assembly and interactions are discussed in terms of their potential role in hereditary retinal diseases.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Goldberg AF,Moritz OL,Molday RSdoi
10.1021/bi00043a028subject
Has Abstractpub_date
1995-10-31 00:00:00pages
14213-9issue
43eissn
0006-2960issn
1520-4995journal_volume
34pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract::The repeating units of mouse, rat, and alpha-monkey satellites have been cloned. All three show properties that are characteristic of curved DNA: (i) their migration in polyacrylamide gels is slower than predicted from their sequences, and (ii) they appear as curved molecules when visualized by electron microscopy. Al...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00461a019
更新日期:1990-03-06 00:00:00
abstract::X-ray crystallographic data have implicated Arg-292 as the residue responsible for the preferred side-chain substrate specificity of aspartate aminotransferase. It forms a salt bridge with the beta or gamma carboxylate group of the substrate [Kirsch, J. F., Eichele, G., Ford, G. C., Vincent, M. G., Jansonius, J. N., G...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00412a052
更新日期:1988-06-14 00:00:00
abstract::Human insulin-like growth factors I and II (hIGF-I, hIGF-II) are potent stimulators of cell and growth processes. They display high sequence similarity to both the A and B chains of insulin but contain an additional connecting C-domain, which reflects their secretion without specific packaging or precursor conversion....
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi020084j
更新日期:2002-07-30 00:00:00
abstract::The repair of the endogenous lesion 8-oxo-7,8-dihydrodeoxyguanosine (8-oxodG) was investigated in the nucleotide excision repair mutant xeroderma pigmentosum D (XPD), using human normal or transformed XPD fibroblasts and the Chinese hamster XPD cell line UV5. In vivo repair of 8-oxodG induced by hydrogen peroxide trea...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi992610l
更新日期:2000-08-29 00:00:00
abstract::Recombination kinetics of CO to protoheme after laser photolysis have been measured vs. temperature and viscosity. A 25-ns laser pulse was focused on the sample to produce an excitation rate of 10(9)/s per heme. This temporarily populates the heme-CO state of dissociated pairs which either separate or recombine on a p...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00358a008
更新日期:1986-05-20 00:00:00
abstract::To probe the mechanism of chaperone substrate selection, we have investigated the kinetics of complex formation and dissociation between the molecular chaperone DnaK and a short peptide (Cro, representing amino acids 1-12 of the cro repressor protein). The Cro protein was N-terminally labeled with the environmentally ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00047a024
更新日期:1995-11-28 00:00:00
abstract::Helices IV and V in the lactose permease of Escherichia coli contain the major determinants for substrate binding [Glu126 (helix IV), Arg144 (helix V), and Cys148 (helix V)]. Structural and dynamic features of this region were studied by using site-directed sulfhydryl modification of 48 single-Cys replacement mutants ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi010866x
更新日期:2001-09-04 00:00:00
abstract::The thermodynamics of the interactions of serum apotransferrin (T) and holotransferrin (TFe(2)) with ceruloplasmin (Cp), as well as those of human lactoferrin (Lf), were assessed by fluorescence emission spectroscopy. Cp interacts with two Lf molecules. The first interaction depends on pH and μ, whereas the second doe...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi1014503
更新日期:2010-12-07 00:00:00
abstract::The noncatalytic beta-subunit of protein kinase CK2 has been shown to display various and in some respects antagonistic effects on the catalytic alpha-subunit [Boldyreff et al. (1993) Biochemistry 32, 12672-12677; Meggio et al. (1994) Biochemistry 33, 4336-4342]. We have now examined the ability of peptides encompassi...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi962885q
更新日期:1997-06-10 00:00:00
abstract::Monoamine oxidase B (MAO B) is an integral protein of the outer mitochondrial membrane that is involved in the deamination of vasoactive and neuroactive amines. The oxidation of these amine substrates requires the cofactor FAD, which is covalently bound to Cys-397 of human MAO B. Previously, Glu-34 and Tyr-44 of MAO B...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9806910
更新日期:1998-09-01 00:00:00
abstract::Oxidative damage by transition metals bound to proteins may be an important pathogenic mechanism. Ceruloplasmin (Cp) is a Cu-containing plasma protein thought to be involved in oxidative modification of lipoproteins. We have previously shown that Cp increased cell-mediated low-density lipoprotein (LDL) oxidation by a ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi981137t
更新日期:1998-10-06 00:00:00
abstract::This work describes the biochemical characterization of the catalytic domain of Ira2p, a Saccharomyces cerevisiae GTPase-activating protein (GAP) regulating the RAS gene products. A fragment of 383 residues (amino acids 1644-2026) was produced in Escherichia coli as glutathione S-transferase fusion protein (GST-Ira2p-...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00042a008
更新日期:1995-10-24 00:00:00
abstract::The mechanisms of interaction of the recombinant N-terminal portion of bactericidal/permeability-increasing protein, rBPI21, with various planar asymmetric and symmetric bilayer membranes, including the lipid matrix of the outer membrane of Gram-negative bacteria, were investigated via electrical measurements. For the...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi970177e
更新日期:1997-08-19 00:00:00
abstract::Big ET-1 and big ET-3 are precursor peptides which render endothelin-1 (ET-1) and endothelin-3 (ET-3) relatively unreactive and resistant to proteolytic cleavage. Big ET-1 is cleaved in vivo by ECE-1 (endothelin-converting enzyme), and big ET-3 is also cleaved but apparently to a significantly lesser extent by this en...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi981689b
更新日期:1999-02-09 00:00:00
abstract::The X-ray structures of the ligand free (apo) and the Mg(2+)*o-succinylbenzoate (OSB) product complex of o-succinylbenzoate synthase (OSBS) from Escherichia coli have been solved to 1.65 and 1.77 A resolution, respectively. The structure of apo OSBS was solved by multiple isomorphous replacement in space group P2(1)2(...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi000855o
更新日期:2000-09-05 00:00:00
abstract::The translocation (T) domain of diphtheria toxin plays a critical role in moving the catalytic domain across the endosomal membrane. Translocation/insertion is triggered by a decrease in pH in the endosome where conformational changes of T domain occur through several kinetic intermediates to yield a final trans-membr...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi500893y
更新日期:2014-11-04 00:00:00
abstract::The two disparate functions of DCoH1 (dimerization cofactor of HNF-1)/PCD (pterin-4a-carbinolamine dehydratase) are associated with a change in oligomeric state. DCoH dimers enhance the activity of the diabetes-associated transcription factor HNF-1α (hepatocyte nuclear factor-1α), while the PCD activity of DCoH1 homot...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi1015056
更新日期:2010-11-30 00:00:00
abstract::Dihydrofolate reductase (DHFR) is often used as a model system to study the relation between protein dynamics and catalysis. We have studied a number of variants of the cold-adapted DHFR from Moritella profunda (MpDHFR), in which the catalytically important M20 and FG loops have been altered, and present a comparison ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi500508z
更新日期:2014-07-29 00:00:00
abstract::We have combined three mutations previously shown to stabilize lambda repressor against thermal denaturation. Two of these mutations are in helix 3, where Gly-46 and Gly-48 have been replaced by alanines [Hecht, M. H., et al. (1986) Proteins: Struct., Funct., Genet. 1, 43-46]. The other mutation, which replaces Tyr-88...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00419a059
更新日期:1988-09-20 00:00:00
abstract::Fluorometric titrations of papain, succinyl-papain, and the corresponding methylthio derivatives of Cys-25 (papain-S-SCH3 and succinyl-papain-S-SCH3) were determined. Removal of the methylthio group from Cys-25 resulted in an increase of approximately 4 pK units in the fluorometrically determined pK value. The corresp...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00504a600
更新日期:1981-01-06 00:00:00
abstract::The binding of bleomycin to DNA in the presence and absence of ferric iron was measured by fluorescence spectroscopy. In millimolar concentrations of tris(hydroxymethyl)aminomethane, pH 7.5, approximately 80% of the bleomycin binds to DNA. Ferric iron seems to have no significant effect on the binding of DNA to bleomy...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00531a021
更新日期:1982-01-19 00:00:00
abstract::The first positive evidence for the utilization of a direct C-6' ' oxidation/reduction mechanism by ADP-l-glycero-d-manno-heptose 6-epimerase is reported here. The epimerase (HldD or AGME, formerly RfaD) operates in the biosynthetic pathway of l-glycero-d-manno-heptose, which is a conserved sugar in the core region of...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi050106c
更新日期:2005-04-19 00:00:00
abstract::As discovered by Grazi & Trombetta [Grazi, E., & Trombetta, G. (1978) Biochem. J. 175, 361], fructose-1,6-bisphosphate aldolase of rabbit muscle causes the slow formation of inorganic phosphate (Pi) and methylglyoxal when incubated with dihydroxyacetone phosphate (DHAP). In addition, these authors found an acid-labile...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00508a026
更新日期:1981-03-03 00:00:00
abstract::The synthesis of sphingomyelin was studied in baby hamster kidney cells and in subcellular fractions derived from rat liver. During pulse-chase experiments with [3H]choline in tissue culture cells, the specific radioactivity of sphingomyelin continued to increase after the specific activities of phosphocholine and cyt...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00540a027
更新日期:1982-05-25 00:00:00
abstract::The determination of solution structures of proteins using two-dimensional NMR data is commonly based on the assumption that the structure can be represented by a single rigid conformer. We present here a procedure whereby this assumption can be relaxed and illustrate its application to acyl carrier protein from Esche...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00448a017
更新日期:1989-10-31 00:00:00
abstract::It has been suggested that the mechanism of alkaline phosphatase (AP) is associative, or triester-like, because phosphorothioate monoesters are hydrolyzed by AP approximately 10(2)-fold slower than phosphate monoesters. This "thio effect" is similar to that observed for the nonenzymatic hydrolysis of phosphate trieste...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi000899x
更新日期:2000-08-08 00:00:00
abstract::CP47 is a pigment-protein complex in the core of photosystem II that tranfers excitation energy to the reaction center. Here we report on a spectroscopic investigation of the isolated CP47 complex. By deconvoluting the 77 K absorption and linear dichroism, red-most states at 683 and 690 nm have been identified with os...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0261948
更新日期:2002-12-24 00:00:00
abstract::The filamentous coliphage M13 possesses multiple copies of a 50-residue coat protein which is inserted into the inner membrane of Escherichia coli during infection. 13C nuclear magnetic resonance (NMR) spectroscopy has been used to probe the structure and dynamics of M13 coat protein solubilized in detergent micelles....
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00351a012
更新日期:1986-02-11 00:00:00
abstract::The self-splicing ai5g group II intron was transformed into a three-part ribozyme that site-specifically cleaves small oligonucleotide substrates with multiple turnover. The ribozyme is composed of intron domain 1 (D1, 425 nucleotides), with catalytically essential domain 5 (D5, 58 nucleotides) provided separately as ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00009a028
更新日期:1995-03-07 00:00:00
abstract::The envelope glycoprotein of HIV-1 consists of the surface subunit gp120 and the transmembrane subunit gp41. Binding of gp120 to target cell receptors induces a conformational change in gp41, which then mediates the fusion of viral and cellular membranes. A buried isoleucine (Ile573) in a central trimeric coiled coil ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0024759
更新日期:2001-03-06 00:00:00