Loop interactions during catalysis by dihydrofolate reductase from Moritella profunda.

abstract：:The crystal structure of a ternary complex of the alcohol dehydrogenase from the archaeon Sulfolobus solfataricus (SsADH) has been determined at 2.3 A. The asymmetric unit contains a dimer with a NADH and a 2-ethoxyethanol molecule bound to each subunit. The comparison with the apo structure of the enzyme reveals that...

journal_title：Biochemistry

authors： Esposito L,Bruno I,Sica F,Raia CA,Giordano A,Rossi M,Mazzarella L,Zagari A

更新日期：2003-12-16 00:00:00

abstract：:We have investigated perturbations of the triplet-state properties of Trp residues in bacteriophage T4 lysozyme caused by point mutations using low-temperature phosphorescence and optical detection of triplet-state magnetic resonance (ODMR) spectroscopy. Five temperature-sensitive mutants have been studied in detail. ...

journal_title：Biochemistry

authors： Zang LH,Ghosh S,Maki AH

更新日期：1989-03-07 00:00:00

abstract：:The specificity of horse liver alcohol dehydrogenase for cyclohexanol and its 3-methyl derivatives was investigated by stopped-flow and initial velocity kinetic studies. The (1S,3S)-3-methylcyclohexanol was 7 times more reactive (V/Km) than cyclohexanol, whereas the (1R,3R)-3-methylcyclohexanol was at least 1000 times...

journal_title：Biochemistry

authors： Lee KM,Dahlhauser KF,Plapp BV

更新日期：1988-05-03 00:00:00

abstract：:Our previous studies demonstrated that the first 1000 amino acid residues (the βα1 domain) of human apolipoprotein (apo) B-100, termed apoB:1000, are required for the initiation of lipoprotein assembly and the formation of a monodisperse stable phospholipid (PL)-rich particle. The objectives of this study were (a) to ...

journal_title：Biochemistry

authors： Manchekar M,Kapil R,Sun Z,Segrest JP,Dashti N

更新日期：2017-08-08 00:00:00

abstract：:Naturally occurring interruptions in nonfibrillar collagen play key roles in molecular flexibility, collagen degradation, and ligand binding. The structural feature of the interruption sequences and the molecular basis for their functions have not been well studied. Here, we focused on a G5G type natural interruption ...

journal_title：Biochemistry

authors： Xu T,Zhou CZ,Xiao J,Liu J

更新日期：2018-02-20 00:00:00

abstract：:The oxidation pathway of chlorophyll Z (ChlZ) in photosystem II (PSII) at cryogenic temperatures was studied by means of light-induced Fourier transform infrared (FTIR) difference spectroscopy. To examine the involvement of redox-active beta-carotene (Car) in the pathway, two Car molecules in Mn-depleted PSII membrane...

journal_title：Biochemistry

authors： Kitajima Y,Noguchi T

更新日期：2006-02-14 00:00:00

abstract：:Several snake venoms contain a phospholipase A2 in which position 49 in the active site is occupied by a lysine or a serine instead of the aspartate residue normally found. Although these proteins do not bind Ca2+ and are devoid of catalytic activity, they are still highly specific myotoxins and have recently been sho...

journal_title：Biochemistry

authors： Pedersen JZ,Lomonte B,Massoud R,Gubensek F,Gutiérrez JM,Rufini S

更新日期：1995-04-11 00:00:00

abstract：:The human LD78 protein (sometimes referred to as human macrophage inflammatory protein-1 alpha) has been shown to protect multipotential hemopoietic stem cells from the effects of cytotoxic agents. Administration of the recombinant stem cell inhibitor molecule LD78 as an adjunct to chemotherapy has potential clinical ...

journal_title：Biochemistry

authors： Patel SR,Evans S,Dunne K,Knight GC,Morgan PJ,Varley PG,Craig S

更新日期：1993-05-25 00:00:00

abstract：:The limited proteolysis approach was used to analyze the conformational features of human growth hormone (hGH) under acidic solvent conditions (A-state). Pepsin was used as the proteolytic probe because of its poor substrate specificity and its activity at low pH. Limited proteolysis of hGH in its A-state results in a...

journal_title：Biochemistry

authors： Spolaore B,Polverino de Laureto P,Zambonin M,Fontana A

更新日期：2004-06-01 00:00:00

abstract：:The phosphorylation of (Na+, K+)ATPase from the electric organ of the electric eel is dependent on Mg2+. The amount of phosphoenzyme formed was increased by K+ and decreased by Na+. Kinetic analyses indicate that a ternary complex of ATPase, Pi and Mg2+ is formed prior to phosphorylation of the protein. Calorimetric s...

journal_title：Biochemistry

authors： Kuriki Y,Halsey J,Biltonen R,Racker E

更新日期：1976-11-16 00:00:00

abstract：:Pf1, a class II filamentous virus, has been investigated by ultraviolet resonance Raman (UVRR) spectroscopy with excitation wavelengths of 257, 244, 238, and 229 nm. The 257-nm UVRR spectrum is rich in Raman bands of the packaged single-stranded DNA (ssDNA) genome, despite the low DNA mass (6%) of the virion. Converse...

journal_title：Biochemistry

authors： Wen ZQ,Armstrong A,Thomas GJ Jr

更新日期：1999-03-09 00:00:00

abstract：:The structures of synthetic peptides corresponding to regions of putative secondary structure in the cellular prion protein PrPC were studied as models for the conformational transition that features in the formation of the pathogenic isoform, PrPSc. Transgenetic studies argue that these PrP isoforms interact during t...

journal_title：Biochemistry

authors： Nguyen J,Baldwin MA,Cohen FE,Prusiner SB

更新日期：1995-04-04 00:00:00

abstract：:Ornithine decarboxylase (ODC) is a pyridoxal 5'-phosphate (PLP) dependent homodimeric enzyme. It is a recognized drug target against African sleeping sickness, caused by Trypanosoma brucei. One of the currently used drugs, alpha-difluoromethylornithine (DFMO), is a suicide inhibitor of ODC. The structure of the T. bru...

journal_title：Biochemistry

authors： Grishin NV,Osterman AL,Brooks HB,Phillips MA,Goldsmith EJ

更新日期：1999-11-16 00:00:00

abstract：:We describe a novel, potent peptide substrate mimetic inhibitor of protein kinase B (PKB/Akt). The compound selectively kills prostate cancer cells, in which PKB is highly activated, but not normal cells, or cancer cells in which PKB is not activated. The inhibitor induces apoptosis and inhibits the phosphorylation of...

journal_title：Biochemistry

authors： Litman P,Ohne O,Ben-Yaakov S,Shemesh-Darvish L,Yechezkel T,Salitra Y,Rubnov S,Cohen I,Senderowitz H,Kidron D,Livnah O,Levitzki A,Livnah N

更新日期：2007-04-24 00:00:00

abstract：:A new method of calculation, based on a direct fitting of the protein fluorescence intensity observed upon coenzyme binding (H.-P. Lutz, unpublished results), is used to study the negative cooperative behavior of glyceraldehyde-3-phosphate dehydrogenase from sturgeon muscle. The calculation procedure simultaneously el...

journal_title：Biochemistry

authors： Branlant G,Eiler B,Biellmann JF,Lutz HP,Luisi PL

更新日期：1983-09-13 00:00:00

abstract：:Bax protein plays a key role in mitochondrial membrane permeabilization and cytochrome c release upon apoptosis. Our recent data have indicated that the 20-residue C-terminal peptide of Bax (BaxC-KK; VTIFVAGVLTASLTIWKKMG), when expressed intracellularly, translocates to the mitochondria and exerts lethal effect on can...

journal_title：Biochemistry

authors： Tatulian SA,Garg P,Nemec KN,Chen B,Khaled AR

更新日期：2012-11-20 00:00:00

abstract：:11Beta-hydroxysteroid dehydrogenase type 1 (11beta-HSD1) catalyzes the conversion of 11-dehydrocorticosterone to its active form corticosterone in rodents (or cortisone to cortisol in humans). The reductive reaction of the 11-keto to 11-hydroxyl is the pivotal switch in the activation of glucocorticoids. An excess of ...

journal_title：Biochemistry

authors： Zhang J,Osslund TD,Plant MH,Clogston CL,Nybo RE,Xiong F,Delaney JM,Jordan SR

更新日期：2005-05-10 00:00:00

abstract：:This paper defines the properties of interaction of endothelin-1 (Et-1) with cloned bovine ETA receptors. The Kd value of Et-1/ETA receptor complexes was estimated in membrane preparations to 20 pM using kinetic experiments and saturation experiments performed under quasi equilibrium conditions. Competition experiment...

journal_title：Biochemistry

authors： Desmarets J,Gresser O,Guedin D,Frelin C

更新日期：1996-11-26 00:00:00

abstract：:Detection of specific reaction products is a powerful approach for dissecting out pathways that mediate oxidative damage in vivo. Eosinophil peroxidase (EPO), an abundant protein secreted from activated eosinophils, has been implicated in promoting oxidative tissue injury in conditions such as asthma, allergic inflamm...

journal_title：Biochemistry

authors： Wu W,Chen Y,d'Avignon A,Hazen SL

更新日期：1999-03-23 00:00:00

abstract：:Globotriaosylceramide [(Gal alpha 1-4Gal beta 1-4Glc-ceramide (Gb3)] was separated from human kidney, and the fatty acid composition was determined. Semisynthetic Gb3 molecular species of corresponding fatty acid chain length were prepared and compared for verotoxin (VT) binding affinity by TLC overlay, and a quantita...

journal_title：Biochemistry

authors： Pellizzari A,Pang H,Lingwood CA

更新日期：1992-02-11 00:00:00

abstract：:Polyclonal antibody to mitochondrial P-450c27/25 reacted with two proteins of apparent molecular masses of 52 kilodaltons (kDa) and 50 kDa from the female rat liver mitochondrial proteins bound to an omega-octylaminoagarose column. The two proteins were purified to greater than 85% homogeneity by DEAE-Sephacel and hyd...

journal_title：Biochemistry

authors： Addya S,Zheng YM,Shayiq RM,Fan JY,Avadhani NG

更新日期：1991-08-27 00:00:00

abstract：:The flexibility and curvature of duplex DNAs containing mismatched sites have been monitored as a function of temperature. The diffusion coefficients are dependent on the flexibility and the curvature of the DNA, and these have been determined by NMR-based methods. The diffusion coefficients, D, depend on a Boltzmann ...

journal_title：Biochemistry

authors： Marathias VM,Jerkovic B,Arthanari H,Bolton PH

更新日期：2000-01-11 00:00:00

abstract：:The temperature dependence of the reduction potential of the CuA site in carbon monoxide inhibited cytochrome c oxidase has been measured with a spectroelectrochemical method adapted to the relatively weak near-infrared absorption of this copper ion. These measurements, together with parallel measurements on the 604-n...

journal_title：Biochemistry

authors： Wang H,Blair DF,Ellis WR Jr,Gray HB,Chan SI

更新日期：1986-01-14 00:00:00

abstract：:Intrastrand cross-links (IaCL) connecting two purine nucleobases in DNA pose a challenge to high-fidelity replication in the cell. Various repair pathways or polymerase bypass can cope with these lesions. The influence of the phosphodiester linkage between two neighboring 2'-deoxyguanosine (dG) residues attached throu...

journal_title：Biochemistry

authors： O'Flaherty DK,Guengerich FP,Egli M,Wilds CJ

更新日期：2015-12-29 00:00:00

abstract：:Nucleosome assembly proteins (Naps) influence chromatin dynamics by directly binding to histones. Here we provide a comprehensive structural and biochemical analysis of a Nap protein from Caenorhabditis elegans (CeNap1). CeNap1 naturally lacks the acidic N-terminal tail and has a short C-terminal tail compared to many...

journal_title：Biochemistry

authors： Sarkar P,Zhang N,Bhattacharyya S,Salvador K,D'Arcy S

更新日期：2019-01-15 00:00:00

abstract：:The two most basic charge isomers of myelin basic protein (BP), components 1 and 2 (C1 and C2), which presumably differ in the degree of deamidation, were purified from bovine BP by cation-exchange chromatography. Two additional specific types of posttranslational modifications were introduced into the purified isomer...

journal_title：Biochemistry

authors： Cheifetz S,Boggs JM,Moscarello MA

更新日期：1985-09-10 00:00:00

abstract：:Dynemicin A (1), a member of the enediyne family of natural products, binds to double-stranded DNA (K(B) approximately 10(4) M(-1)) and in the presence of millimolar concentrations of a reducing cofactor such as NADPH or GSH reacts to cleave DNA. In this work, we show that the two flavin-based enzymes ferredoxin-NADP+...

journal_title：Biochemistry

authors： Myers AG,Kort ME,Cohen SB,Tom NJ

更新日期：1997-04-01 00:00:00

abstract：:Biotin synthase catalyzes the conversion of dethiobiotin (DTB) to biotin through the oxidative addition of sulfur between two saturated carbon atoms, generating a thiophane ring fused to the existing ureido ring. Biotin synthase is a member of the radical SAM superfamily, composed of enzymes that reductively cleave S-...

journal_title：Biochemistry

authors： Taylor AM,Stoll S,Britt RD,Jarrett JT

更新日期：2011-09-20 00:00:00

abstract：:Carbonic anhydrase V (CA V) is a mitochondrial enzyme that catalyzes the hydration of CO2 to produce bicarbonate and a proton. The catalytic properties of wild-type murine CA V suggest the presence of a proton shuttle residue having pKa = 9.2, the role of which is to transfer a proton from zinc-bound water to solution...

journal_title：Biochemistry

authors： Heck RW,Boriack-Sjodin PA,Qian M,Tu C,Christianson DW,Laipis PJ,Silverman DN

更新日期：1996-09-10 00:00:00

abstract：:The fluorescence quenching of 9-aminoacridine (9AA) in suspension of beef heart EDTA submitochondrial particles was studied and was used to calculate the pH gradient between these particles and the medium. This pH gradient, which is energy dependent, is also dependent strongly on the presence of anion species in the m...

journal_title：Biochemistry

authors： Rottenberg H,Lee CP

更新日期：1975-06-17 00:00:00