Reactivity of horse liver alcohol dehydrogenase with 3-methylcyclohexanols.

abstract：:A single G3:U70 base pair in the acceptor helix is the major determinant of alanine acceptance in alanine transfer RNAs. Transfer of this base pair into other transfer RNAs confers alanine acceptance. A G3:C70 substitution eliminates alanine acceptance in vivo and in vitro. In this work, a population of mutagenized G3...

journal_title：Biochemistry

authors： Hou YM,Schimmel P

更新日期：1992-10-27 00:00:00

abstract：:The modified Tanford-Kirkwood electrostatic theory (Shire et al., 1974a) was applied to ferrimyoglobins from the following animal species: sperm whale (Physeter catodon), horse, California grey whale (Eschrichtius gibbosus), harbor seal (Phoca vitulina), and California sea lion (Zalophus californianus). Computations w...

journal_title：Biochemistry

authors： Shire SJ,Hanania GI,Gurd FR

更新日期：1975-04-08 00:00:00

abstract：:Dihydrofolate and dihydropteroylpolyglutamates inhibit pig liver methylenetetrahydrofolate reductase. In all cases the inhibition is linearly competitive with respect to methylenetetrahydrofolate. The Ki values decrease with each additional glutamyl residue from one to six, from a value of 6.5 microM for dihydrofolate...

journal_title：Biochemistry

authors： Matthews RG,Baugh CM

更新日期：1980-05-13 00:00:00

abstract：:The relaxin-like factor (RLF) is a circulating hormone that is synthesized in the gonads of mammals and released into the bloodstream. The distribution of its receptor and a trace of cross-reactivity to relaxin receptors implied that this relatively new factor is more relaxin- than insulin-like. The chemical synthesis...

journal_title：Biochemistry

authors： Büllesbach EE,Schwabe C

更新日期：1999-03-09 00:00:00

abstract：:A cationic, copper(II)-containing ligand, derived from bulky 5,10,15,20-tetrakis(N-methylpyridinium-4-yl)porphyrin, Cu(T4), and two sterically friendlier forms, [trans-5,15-di(N-methylpyridinium-4-yl)porphyrinato]copper(II), Cu(tD4), and [cis-5,10-di(N-methylpyridinium-4-yl)porphyrinato]copper(II), Cu(cD4), bind to DN...

journal_title：Biochemistry

authors： Briggs BN,Gaier AJ,Fanwick PE,Dogutan DK,McMillin DR

更新日期：2012-09-25 00:00:00

abstract：:Two-dimensional homonuclear and heteronuclear NMR and minimized potential energy calculations have been combined to define the structure of the antitumor agent mitomycin C (MC) cross-linked to deoxyguanosines on adjacent base pairs in the d(T1-A2-C3-G4-T5-A6).d(T7-A8-C9-G10-T11-A12) duplex. The majority of the mitomyc...

journal_title：Biochemistry

authors： Norman D,Live D,Sastry M,Lipman R,Hingerty BE,Tomasz M,Broyde S,Patel DJ

更新日期：1990-03-20 00:00:00

abstract：:Extensive molecular modeling with molecular dynamics simulations and van der Waals energy analyses were used to elucidate the striking finding that a mutagenic benzo[a]pyrene-derived DNA lesion, the base-displaced intercalated 10R-(+)-cis-anti-B[a]P-N(2)-dG (G*), manifests large differences in nucleotide excision repa...

journal_title：Biochemistry

authors： Mu H,Kropachev K,Chen Y,Zhang H,Cai Y,Geacintov NE,Broyde S

更新日期：2013-08-20 00:00:00

abstract：:The difference spectra of binary and ternary complexes of horse liver alcohol dehydrogenase with oxidized and reduced nicotinamide adenine dinucleotides, nicotinamide 1,N6-ethenoadenine dinucleotide, and adenosine diphosphate ribose along with a number of substrate analogues have been measured. These spectra bear a ve...

journal_title：Biochemistry

authors： Subramanian S,Ross JB,Ross PD,Brand L

更新日期：1981-07-07 00:00:00

abstract：:Sarcoplasmic phosphorylase phosphatase extracted from ground skeletal muscle was recovered in a high molecular weight from (Mr = 250000). This enzyme has been purified from extracts by anion-exchange and gel chromatography to yield a preparation with three major protein components of Mr 83000, 72000, and 32000 by sodi...

journal_title：Biochemistry

authors： Brautigan DL,Picton C,Fischer EH

更新日期：1980-12-09 00:00:00

abstract：:In refolding of iso-2 cytochrome c from Saccharomyces cerevisiae, there are two slow folding reactions, tau 1a and tau 1b. The slower of the slow reactions, tau 1a = 100-200 s, is observed only by absorbance changes, while tau 1b (10-20-fold faster) is detected by fluorescence changes. The temperature dependence of th...

journal_title：Biochemistry

authors： Osterhout JJ Jr,Nall BT

更新日期：1985-12-31 00:00:00

abstract：:The malarial parasite Plasmodium falciparum can only synthesize pyrimidine nucleotides via the de novo pathway which is therefore a suitable target for development of antimalarial drugs. New assay procedures have been developed using high-pressure liquid chromatography (HPLC) which enable concurrent measurement of pyr...

journal_title：Biochemistry

authors： Seymour KK,Lyons SD,Phillips L,Rieckmann KH,Christopherson RI

更新日期：1994-05-03 00:00:00

abstract：:Formylglycinamide ribonucleotide amidotransferase (FGAR-AT) catalyzes the ATP- and glutamine-dependent formation of formylglycinamidine ribonucleotide, ADP, P(i), and glutamate in the fourth step of de novo purine biosynthesis. Like all amidotransferases (ATs), FGAR-AT is proposed to channel ammonia between a glutamin...

journal_title：Biochemistry

authors： Hoskins AA,Anand R,Ealick SE,Stubbe J

更新日期：2004-08-17 00:00:00

abstract：:Inflammation and subsequent cyclooxygenase-2 (COX-2) activity has long been linked with the development of cancer, although little is known about any epigenetic effects of COX-2. A product of COX-2 activation, levuglandin (LG) quickly forms covalent bonds with nearby primary amines, such as those in lysine, which lead...

journal_title：Biochemistry

authors： Carrier EJ,Zagol-Ikapitte I,Amarnath V,Boutaud O,Oates JA

更新日期：2014-04-22 00:00:00

abstract：:Two dioxygenases (ARD and ARD') were cloned from Klebsiella pneumoniae that catalyze different oxidative decomposition reactions of an advanced aci-reductone intermediate, CH(3)SCH(2)CH(2)COCH(OH)=CH(OH) (I), in the methionine salvage pathway. The two enzymes are remarkable in that they have the same polypeptide seque...

journal_title：Biochemistry

authors： Dai Y,Pochapsky TC,Abeles RH

更新日期：2001-05-29 00:00:00

abstract：:A series of diastereomeric dipeptide amides, containing an N-terminal L-lysyl residue and a C-terminal L- or D-amino acid with a derivatized aromatic ring on the side chain, was synthesized to determine the dependence of (1) the chirality of the N-terminal amino acid alpha-carbon and (2) the length of the N-terminal a...

journal_title：Biochemistry

authors： Sheardy RD,Gabbay EJ

更新日期：1983-04-26 00:00:00

abstract：:Light-induced activation of the LOV2-Jα domain of the photoreceptor phototropin from oat is believed to involve the detachment of the Jα helix from the central β-sheet and its subsequent unfolding. The dynamics of these conformational changes were monitored by time-resolved emission spectroscopy with 100 ns time resol...

journal_title：Biochemistry

authors： Hoersch D,Bolourchian F,Otto H,Heyn MP,Bogomolni RA

更新日期：2010-12-28 00:00:00

abstract：:The Haemophilus influenzae diaminopimelate epimerase was cloned, expressed, purified, and crystallized in the C2221 space group (a = 102.1 A, b = 115.4 A, c = 66.3 A, alpha = beta = gamma = 90 degrees). The three-dimensional structure was solved to 2.7 A using a single Pt derivative and the Se-Met-substituted enzyme t...

journal_title：Biochemistry

authors： Cirilli M,Zheng R,Scapin G,Blanchard JS

更新日期：1998-11-24 00:00:00

abstract：:Purine nucleoside phosphorylase (PNP) catalyzes the reversible phosphorolysis of purine ribonucleosides and 2'-deoxyribonucleosides, yielding the purine base and (2'-deoxy)ribose 1-phosphate as products. While this enzyme has been extensively studied, several questions with respect to the catalytic mechanism have rema...

journal_title：Biochemistry

authors： Isaksen GV,Hopmann KH,Åqvist J,Brandsdal BO

更新日期：2016-04-12 00:00:00

abstract：:Runx proteins constitute a family of mammalian transcription factors that interact with DNA through their evolutionarily conserved Runt domain. CBFbeta, alternatively denoted PEBP2beta, is the non-DNA-binding heterodimer partner and acts to enhance the DNA binding affinity of Runx proteins. Runx proteins and CBFbeta a...

journal_title：Biochemistry

authors： Wolf-Watz M,Grundström T,Härd T

更新日期：2001-09-25 00:00:00

abstract：:Unilamellar liposomes can be fused at low pH with the plasma membrane of cells that express the hemagglutinin glycoprotein of influenza virus on their surface [van Meer, G., & Simons, K. (1983) J. Cell Biol. 97, 1365-1374]. Here, we have resolved this fusion process into two kinetically distinct steps. The first and m...

journal_title：Biochemistry

authors： van Meer G,Davoust J,Simons K

更新日期：1985-07-02 00:00:00

abstract：:The specific rate of superoxide (O2(•-)) production in the purified active crystallizable cytochrome b6f complex, normalized to the rate of electron transport, has been found to be more than an order of magnitude greater than that measured in isolated yeast respiratory bc1 complex. The biochemical and structural basis...

journal_title：Biochemistry

authors： Baniulis D,Hasan SS,Stofleth JT,Cramer WA

更新日期：2013-12-17 00:00:00

abstract：:The mammalian HIRA/UBN1/CABIN1/ASF1a (HUCA) histone chaperone complex deposits the histone H3 variant H3.3 into chromatin and is linked to gene activation, repression, and chromatin assembly in diverse cell contexts. We recently reported that a short N-terminal fragment of UBN1 containing amino acids 1-175 is necessar...

journal_title：Biochemistry

authors： Tang Y,Puri A,Ricketts MD,Rai TS,Hoffmann J,Hoi E,Adams PD,Schultz DC,Marmorstein R

更新日期：2012-03-27 00:00:00

abstract：:Murine double-minute clone 2 protein (MDM2) is an E3 ubiquitin ligase that regulates the turnover of several cellular factors including the p53 tumor suppressor protein. As part of the mechanism of p53 induction in response to DNA damage, a cluster of serine residues within the central acidic domain of MDM2 become hyp...

journal_title：Biochemistry

authors： Winter M,Milne D,Dias S,Kulikov R,Knippschild U,Blattner C,Meek D

更新日期：2004-12-28 00:00:00

abstract：:Human immunodeficiency viruses type 1 and 2 (HIV-1 and HIV-2, respectively) exhibit extensive genetic variations. It was postulated that much of this genetic variability stems from the low fidelity of the reverse transcription step. Both HIV reverse transcriptases (RTs) were shown to be particularly error-prone during...

journal_title：Biochemistry

authors： Bakhanashvili M,Hizi A

更新日期：1992-10-06 00:00:00

abstract：:Perturbations within the transactivation and carboxy-terminal domains of HIV-1 Rev were examined for effects on Rev responsive element (RRE) binding activities in vitro and biological activity in vivo. Binding affinities, specificities, and multimerization of the transactivation mutants M10 and Rev/Rex M10-16 on the R...

journal_title：Biochemistry

authors： Daly TJ,Rennert P,Lynch P,Barry JK,Dundas M,Rusche JR,Doten RC,Auer M,Farrington GK

更新日期：1993-08-31 00:00:00

abstract：:The cytidine repressor (CytR) is a member of the LacR family of bacterial repressors with distinct functional features. The Escherichia coli CytR regulon comprises nine operons whose palindromic operators vary in both sequence and, most significantly, spacing between the recognition half-sites. This suggests a strong ...

journal_title：Biochemistry

authors： Moody CL,Tretyachenko-Ladokhina V,Laue TM,Senear DF,Cocco MJ

更新日期：2011-08-09 00:00:00

abstract：:Rat mucosal-type mast cells of the RBL-2H3 line express a glycoprotein termed the MAst cell Function-associated Antigen (MAFA). When MAFA is clustered by its specific monoclonal antibody G63, secretion normally triggered by aggregating these cells' type I Fcepsilon receptor (FcepsilonRI) is substantially inhibited. Th...

journal_title：Biochemistry

authors： Song J,Hagen GM,Roess DA,Pecht I,Barisas BG

更新日期：2002-01-22 00:00:00

abstract：:A site-specific analog of ATP, 6,6'-dithiobis (inosinyl imidodiphosphate (S2P-PNP), inactivates the ATPase activities of myosin's proteolytic fragments, heavy meromyosin (HMM) and subfragment one (SF1), by formation of mixed disulfides between the 6 position of the purine ring and certain key cysteines. The stoichiome...

journal_title：Biochemistry

authors： Wagner PD,Yount RG

更新日期：1975-05-06 00:00:00

abstract：:Although G protein-coupled receptor (GPCR) kinases (GRKs) have been shown to mediate desensitization of numerous GPCRs in studies using cellular expression systems, their function under physiological conditions is less well understood. In the current study, we employed various strategies to assess the effect of inhibi...

journal_title：Biochemistry

authors： Kong KC,Gandhi U,Martin TJ,Anz CB,Yan H,Misior AM,Pascual RM,Deshpande DA,Penn RB

更新日期：2008-09-02 00:00:00

abstract：:The structural domains of human apolipoprotein(a) (apo(a)) in the lipoprotein(a) (Lp(a)) particle have been recently investigated by limited proteolysis [Huby, T., Doucet, C., Dieplinger, H., Chapman, J., & Thillet, J. (1994) Biochemistry 33, 3335-3341]. We have shown that apo(a) can be cleaved into two structural dom...

journal_title：Biochemistry

authors： Huby T,Schröder W,Doucet C,Chapman J,Thillet J

更新日期：1995-06-06 00:00:00