Abstract：
:The temperature dependence of the reduction potential of the CuA site in carbon monoxide inhibited cytochrome c oxidase has been measured with a spectroelectrochemical method adapted to the relatively weak near-infrared absorption of this copper ion. These measurements, together with parallel measurements on the 604-nm absorption due to Fea, indicate that an interaction between CuA and Fea causes the reduction potential for one of these sites to be decreased by approximately 40 mV upon reduction of the other. The temperature dependence of the CuA reduction potential indicates a relatively large and negative standard entropy of reduction of CuA (delta So' = -48.7 +/- 2.3 eu). Possible implications of the intersite redox interaction and the large standard entropy of reduction of the CuA site are discussed.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Wang H,Blair DF,Ellis WR Jr,Gray HB,Chan SIdoi
10.1021/bi00349a024subject
Has Abstractpub_date
1986-01-14 00:00:00pages
167-71issue
1eissn
0006-2960issn
1520-4995journal_volume
25pub_type
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