Immunochemical analysis of membrane vesicles from Escherichia coli.

abstract：:In the yeast Saccharomyces cerevisiae, CTP synthetase [EC 6.3.4.2; UTP:ammonia ligase (ADP-forming)] is the product of the URA7 gene. CTP synthetase was purified 503-fold to apparent homogeneity from cells bearing the URA7 gene on a multicopy plasmid that directed a 10-fold overproduction of the enzyme. The purificati...

journal_title：Biochemistry

authors： Yang WL,McDonough VM,Ozier-Kalogeropoulos O,Adeline MT,Flocco MT,Carman GM

更新日期：1994-09-06 00:00:00

abstract：:The interaction of nile red (NR) with apomyoglobin (ApoMb) in the native (pH 7) and molten globule (pH 4) states was investigated using experimental and computational methods. NR binds to hydrophobic locations in ApoMb with higher affinity (K(d) = 25 +/- 5 microM) in the native state than in the molten globule state (...

journal_title：Biochemistry

authors： Polverini E,Cugini G,Annoni F,Abbruzzetti S,Viappiani C,Gensch T

更新日期：2006-04-25 00:00:00

abstract：:Lipid-protein interactions in bovine rod outer segment disk membranes have been studied by using a series of eight stearic acid spin-label probes which were labeled at different carbon atom positions in the chain. In randomly oriented membrane dispersions, the electron spin resonance (ESR) spectra of the C-8, C-9, C-1...

journal_title：Biochemistry

authors： Pates RD,Marsh D

更新日期：1987-01-13 00:00:00

abstract：:Rhodopsin is the photoreceptor in vertebrate rod cells responsible for vision at low light intensities. The photoreactive chromophore in rhodopsin is 11-cis-retinal bound to the protein via a protonated Schiff base with Glu113 as the counterion. We have used the observed 13C NMR chemical shifts of the conjugated retin...

journal_title：Biochemistry

authors： Han M,Smith SO

更新日期：1995-01-31 00:00:00

abstract：:Trypanosomes are purine-auxotrophic parasites that depend upon nucleoside hydrolase (NH) activity to salvage nitrogenous bases necessary for nucleic acid and cofactor synthesis. Nonspecific and purine-specific NHs have been widely studied, yet little is known about the 6-oxopurine-specific isozymes, although they are ...

journal_title：Biochemistry

authors： Vandemeulebroucke A,Minici C,Bruno I,Muzzolini L,Tornaghi P,Parkin DW,Versées W,Steyaert J,Degano M

更新日期：2010-10-19 00:00:00

abstract：:Marijuana is the most widely abused illegal drug, and its spectrum of effects suggests that several receptors are responsible for the activity. Two cannabinoid receptor subtypes, CB1 and CB2, have been identified, but the complex pharmacological properties of exogenous cannabinoids and endocannabinoids are not fully e...

journal_title：Biochemistry

authors： Kotsikorou E,Madrigal KE,Hurst DP,Sharir H,Lynch DL,Heynen-Genel S,Milan LB,Chung TD,Seltzman HH,Bai Y,Caron MG,Barak L,Abood ME,Reggio PH

更新日期：2011-06-28 00:00:00

abstract：:A general, combinatorial library method for the rapid identification of high-affinity peptide ligands of protein modular domains is reported. The validity of this method has been demonstrated by determining the sequence specificity of four Src homology 2 (SH2) domains derived from protein tyrosine phosphatase SHP-1 an...

journal_title：Biochemistry

authors： Sweeney MC,Wavreille AS,Park J,Butchar JP,Tridandapani S,Pei D

更新日期：2005-11-15 00:00:00

abstract：:Pokeweed antiviral protein (PAP) is a ribosome inactivating protein (RIP) that depurinates the sarcin/ricin loop (SRL) of rRNA, inhibiting protein synthesis. PAP depurinates viral RNA, and in doing so, lowers the infectivity of many plant viruses. The mechanism by which PAP accesses uncapped viral RNA is not known, im...

journal_title：Biochemistry

authors： Domashevskiy AV,Williams S,Kluge C,Cheng SY

更新日期：2017-11-14 00:00:00

abstract：:The steady-state and pre-steady-state kinetic parameters for the interaction of E with the fluorogenic substrate 2-aminobenzoyl-Thr-Ile-Nle-Phe(p-NO(2))-Gln-Arg-NH(2) were determined in 1.25 M NaCl, 0.1 M MES-TRIS at pH 6.0 at 25 degrees C. At low concentrations of enzyme, the values of the K(m) and k(cat) calculated ...

journal_title：Biochemistry

authors： Porter DJ,Hanlon MH,Furfine ES

更新日期：2002-01-29 00:00:00

abstract：:The inhibitory effects of urea on the normally rapid fibrillation of human calcitonin (hCT) were investigated by 1H NMR. From subtle differences in the chemical shift of hCT in the presence and absence of urea, the occurrence of weak interactions between urea and hCT was confirmed. The chemical shifts on the NH and C ...

journal_title：Biochemistry

authors： Kanaori K,Nosaka AY

更新日期：1996-10-01 00:00:00

abstract：:The only identified component of the scrapie prion is PrPSc, a glycosylinositol phospholipid (GPI)-linked protein that is derived from the cellular isoform (PrPC) by an as yet unknown posttranslational event. Analysis of the PrPSc GPI has revealed six different glycoforms, three of which are unprecedented. Two of the ...

journal_title：Biochemistry

authors： Stahl N,Baldwin MA,Hecker R,Pan KM,Burlingame AL,Prusiner SB

更新日期：1992-06-02 00:00:00

abstract：:RecG is a DNA helicase involved in the repair of damage at a replication fork and catalyzes the reversal of the fork to a point beyond the damage in the template strand. It unwinds duplex DNA in reactions that are coupled to ATP hydrolysis. The kinetic mechanism of duplex DNA unwinding by RecG was analyzed using a qua...

journal_title：Biochemistry

authors： Martinez-Senac MM,Webb MR

更新日期：2005-12-27 00:00:00

abstract：:Human prolactin (hPRL) binds two human prolactin receptor molecules, creating active heterotrimeric complexes. Receptors bind dissimilar hormone surfaces termed site 1 and site 2 in an obligate ordered process. We sought to map the functional epitopes in site 1 of hPRL. Extensive alanine mutagenesis (102 of the 199 re...

journal_title：Biochemistry

authors： Rao GV,Brooks CL

更新日期：2011-03-01 00:00:00

abstract：:Studies of ligand binding to acetylcholinesterase (AChE) have demonstrated two sites of interaction. An acyl-enzyme intermediate is formed at the acylation site, and catalytic activity can be inhibited by ligand binding to a peripheral site. The three-dimensional structures of AChE-ligand complexes reveal a narrow and...

journal_title：Biochemistry

authors： Mallender WD,Szegletes T,Rosenberry TL

更新日期：2000-07-04 00:00:00

abstract：:Phospholipase A(2) (PLA(2)) (E. C. 3.1.1.4) is a common enzyme in the two-way cascade mechanism leading to the production of proinflammatory compounds known as eicosanoids. The binding of phospholipase A(2) to the membrane surface and hydrolysis of phospholipids are thought to involve the formation of a hydrophobic ch...

journal_title：Biochemistry

authors： Singh RK,Vikram P,Makker J,Jabeen T,Sharma S,Dey S,Kaur P,Srinivasan A,Singh TP

更新日期：2003-10-14 00:00:00

abstract：:CRISPR-Cas9 technology has been at the forefront of the field of biology. The Streptococcus pyogenes (SpyCas9) protein forms a complex with guide RNA and can recognize and cleave double-stranded DNA through hybridization based on 20 base pairings. SpyCas9 has two nuclease domains, HNH and RuvC, each of which cuts each...

journal_title：Biochemistry

authors： Furuhata Y,Kato Y

更新日期：2021-01-26 00:00:00

abstract：:Coenzyme A (CoASH) is the major low-molecular weight thiol in Staphylococcus aureus and a number of other bacteria; the crystal structure of the S. aureus coenzyme A-disulfide reductase (CoADR), which maintains the reduced intracellular state of CoASH, has recently been reported [Mallett, T.C., Wallen, J.R., Karplus, ...

journal_title：Biochemistry

authors： Nicely NI,Parsonage D,Paige C,Newton GL,Fahey RC,Leonardi R,Jackowski S,Mallett TC,Claiborne A

更新日期：2007-03-20 00:00:00

abstract：:Recently, we revealed the architecture of the clamp-loader-helicase (tau-DnaB) complex in Bacillus by atomic force microscopy imaging and constructed a structural model, whereby a pentameric clamp-loader interacts with the hexameric helicase. Crucial to this model is the assumption that the clamp-loader forms a pentam...

journal_title：Biochemistry

authors： Haroniti A,Till R,Smith MC,Soultanas P

更新日期：2003-09-23 00:00:00

abstract：:Human type beta 2 transforming growth factor (hTGF-beta 2) was purified from tamoxifen-supplemented, serum-free medium conditioned by the human prostatic adenocarcinoma cell line PC-3. The purification of hTGF-beta 2 was monitored in a growth inhibition assay and was achieved by batch purification on methylsilyl-contr...

journal_title：Biochemistry

authors： Ikeda T,Lioubin MN,Marquardt H

更新日期：1987-05-05 00:00:00

abstract：:The three-dimensional structure of d[CGTA'CG], where A' = [2-NH2-A], was determined to atomic (1.35 A) resolution by single isomorphous replacement. The d[CGTA'CG] hexamer crystallizes in space group P3221, and is not isomorphous with other DNA hexanucleotides. Despite completely different crystal packing, the essenti...

journal_title：Biochemistry

authors： Parkinson GN,Arvanitis GM,Lessinger L,Ginell SL,Jones R,Gaffney B,Berman HM

更新日期：1995-11-28 00:00:00

abstract：:Tau is a microtubule associated protein implicated in the pathogenesis of several neurodegenerative diseases. Because of the channel forming properties of other amyloid peptides, we employed planar lipid bilayers and atomic force microscopy to test tau for its ability to form ion permeable channels. Our results demons...

journal_title：Biochemistry

authors： Patel N,Ramachandran S,Azimov R,Kagan BL,Lal R

更新日期：2015-12-22 00:00:00

abstract：:Angiotensin converting enzyme (ACE) plays a critical role in the circulating or endocrine renin-angiotensin system (RAS) as well as the local regulation that exists in tissues such as the myocardium and skeletal muscle. Here we report the high-resolution crystal structures of testis ACE (tACE) in complex with the firs...

journal_title：Biochemistry

authors： Natesh R,Schwager SL,Evans HR,Sturrock ED,Acharya KR

更新日期：2004-07-13 00:00:00

abstract：:The thermotropic behavior of 20 chemically related glycosphingolipids (GSLs) of high purity, containing neutral and anionic carbohydrate residues in their oligosaccharide chains, was studied by high-sensitivity differential scanning calorimetry. In general, the polar head group of GSLs appears to be one of the major d...

journal_title：Biochemistry

authors： Maggio B,Ariga T,Sturtevant JM,Yu RK

更新日期：1985-02-26 00:00:00

abstract：:Hormone-sensitive lipase (HSL) is a rate-limiting enzyme in lipolysis that displays broad substrate specificity. HSL function is regulated by reversible phosphorylation that occurs within a 150 aa "regulatory module" of the protein. The current studies used mutational analysis to dissect the contribution of the "regul...

journal_title：Biochemistry

authors： Wang J,Shen WJ,Patel S,Harada K,Kraemer FB

更新日期：2005-02-15 00:00:00

abstract：:Membrane-bound guanylate cyclases harbor a region called the dimerization or linker domain, which aids the enzymes in adopting an optimal monomer-monomer arrangement for catalysis. One subgroup of these guanylate cyclases is expressed in rod and cone cells of vertebrate retina, and mutations in the dimerization domain...

journal_title：Biochemistry

authors： Zägel P,Dell'Orco D,Koch KW

更新日期：2013-07-30 00:00:00

abstract：:Mammalian thioredoxin reductase (TR) is a pyridine nucleotide disulfide oxidoreductase that uses the rare amino acid selenocysteine (Sec) in place of the more commonly used amino acid cysteine (Cys) in the redox-active tetrapeptide Gly-Cys-Sec-Gly motif to catalyze thiol/disulfide exchange reactions. Sec can accelerat...

journal_title：Biochemistry

authors： Lothrop AP,Snider GW,Ruggles EL,Patel AS,Lees WJ,Hondal RJ

更新日期：2014-02-04 00:00:00

abstract：:Spike glycoprotein of SARS coronavirus (S protein) plays a pivotal role in SARS coronavirus (SARS_CoV) infection. The immunological fragment of the S protein (Ala251-His641, SARS_S1b) is believed to be essential for SARS_CoV entering the host cell through S protein-ACE-2 interaction. We have quantitatively characteriz...

journal_title：Biochemistry

authors： Yu C,Gui C,Luo H,Chen L,Zhang L,Yu H,Yang S,Jiang W,Shen J,Shen X,Jiang H

更新日期：2005-02-08 00:00:00

abstract：:The dissociation of the trypsin-sensitive basic tails of the core histones in core chromatin has been followed as a function of [NaCl] using proton NMR spectroscopy. The tails dissociate in a highly cooperative all or none manner over the salt concentration range 0.2-0.6 M, that is, below the salt concentration requir...

journal_title：Biochemistry

authors： Walker IO

更新日期：1984-11-06 00:00:00

abstract：:Topoisomerase II mediated double-strand breaks produced by anthracycline analogues were studied in SV40 DNA. The compounds included doxorubicin, daunorubicin, two doxorubicin stereoisomers (4'-epimer and beta-anomer), and five chromophore-modified derivatives, with a wide range of cytotoxic activity and DNA binding af...

journal_title：Biochemistry

authors： Capranico G,Zunino F,Kohn KW,Pommier Y

更新日期：1990-01-16 00:00:00

abstract：:Light-induced GTP-dependent scattering changes are studied in suspensions of retinal disc membranes to which one or both of the purified proteins involved in the phototransduction mechanism (G-protein and cGMP phosphodiesterase) are reassociated; a scattering change which depends on the presence of both G-protein (G) ...

journal_title：Biochemistry

authors： Bennett N,Clerc A

更新日期：1992-02-18 00:00:00