cGMP phosphodiesterase dependent light-induced scattering changes in suspensions of retinal disc membranes.

abstract：:We have studied the structure of the CuB site in the binuclear heme-copper center of the fully oxidized form of the quinol-oxidizing cytochrome aa3-600 from Bacillus subtilis by EXAFS and ENDOR spectroscopy. This enzyme is member of the large superfamily of heme-copper respiratory oxidases, which catalyze the reductio...

journal_title：Biochemistry

authors： Fann YC,Ahmed I,Blackburn NJ,Boswell JS,Verkhovskaya ML,Hoffman BM,Wikström M

更新日期：1995-08-15 00:00:00

abstract：:The two disparate functions of DCoH1 (dimerization cofactor of HNF-1)/PCD (pterin-4a-carbinolamine dehydratase) are associated with a change in oligomeric state. DCoH dimers enhance the activity of the diabetes-associated transcription factor HNF-1α (hepatocyte nuclear factor-1α), while the PCD activity of DCoH1 homot...

journal_title：Biochemistry

authors： Rho H,Jones CN,Rose RB

更新日期：2010-11-30 00:00:00

abstract：:The cytochrome bc(1) complex from Paracoccus denitrificans and soluble fragments of its cytochrome c(1) and Rieske ISP subunits are characterized by a combined approach of protein electrochemistry and FTIR difference spectroscopy. The FTIR difference spectra provide information about alterations in the protein upon re...

journal_title：Biochemistry

authors： Ritter M,Anderka O,Ludwig B,Mäntele W,Hellwig P

更新日期：2003-10-28 00:00:00

abstract：:The stereochemistry of proton transfer in the isomerization of [4 beta-2H]-5-androstene-3,17- dione (1d) to 4-androstene-3,17-dione (3) catalyzed by 3-oxo-delta 5-steroid isomerase (KSI) has been reinvestigated. In H2O, approximately 65% of the label is retained in the product (3); of this, one-third is at C-4 and two...

journal_title：Biochemistry

authors： Zawrotny ME,Hawkinson DC,Blotny G,Pollack RM

更新日期：1996-05-21 00:00:00

abstract：:In an earlier study, β³-puromycin was used for the selection of modified ribosomes, which were utilized for the incorporation of five different β-amino acids into Escherichia coli dihydrofolate reductase (DHFR). The selected ribosomes were able to incorporate structurally disparate β-amino acids into DHFR, in spite of...

journal_title：Biochemistry

authors： Maini R,Chowdhury SR,Dedkova LM,Roy B,Daskalova SM,Paul R,Chen S,Hecht SM

更新日期：2015-06-16 00:00:00

abstract：:Terreic acid is a metabolite with antibiotic properties produced by the fungus Aspergillus terreus. We found that terreic acid is a covalent inhibitor of the bacterial cell wall biosynthetic enzyme MurA from Enterobacter cloacae and Escherichia coli in vitro. The crystal structure of the MurA dead-end complex with ter...

journal_title：Biochemistry

authors： Han H,Yang Y,Olesen SH,Becker A,Betzi S,Schönbrunn E

更新日期：2010-05-18 00:00:00

abstract：:We have de novo designed a heterodimeric coiled-coil formed by two peptides as a capture/delivery system that can be used in applications such as affinity tag purification, immobilization in biosensors, etc. The two strands are designated as K coil (KVSALKE heptad sequence) and E coil (EVSALEK heptad sequence), where ...

journal_title：Biochemistry

authors： De Crescenzo G,Litowski JR,Hodges RS,O'Connor-McCourt MD

更新日期：2003-02-18 00:00:00

abstract：:Ferrochelatase is the terminal enzyme in the heme biosynthetic pathway. It catalyzes the insertion of ferrous iron into protoporphyrin IX to produce protoheme IX. The crystal structures of ferrochelatase from Saccharomyces cerevisiae in free form, in complex with Co(II), a substrate metal ion, and in complex with two ...

journal_title：Biochemistry

authors： Karlberg T,Lecerof D,Gora M,Silvegren G,Labbe-Bois R,Hansson M,Al-Karadaghi S

更新日期：2002-11-19 00:00:00

abstract：:Covalent labeling of the canine renal parathyroid hormone receptor with [125I]bPTH(1-34) reveals several major binding components that display characteristics consistent with a physiologically relevant adenylate cyclase linked receptor. Through the use of the specific glycosidases neuraminidase and endoglycosidase F a...

journal_title：Biochemistry

authors： Karpf DB,Arnaud CD,King K,Bambino T,Winer J,Nyiredy K,Nissenson RA

更新日期：1987-12-01 00:00:00

abstract：:Human melanin-concentrating hormone (hMCH) and many of its analogues are potent but nonspecific ligands for human melanin-concentrating hormone receptors 1 and 2 (hMCH-1R and hMCH-2R). To differentiate between the physiological functions of these receptors, selective antagonists are needed. In this study, analogues of...

journal_title：Biochemistry

authors： Bednarek MA,Hreniuk DL,Tan C,Palyha OC,MacNeil DJ,Van der Ploeg LH,Howard AD,Feighner SD

更新日期：2002-05-21 00:00:00

abstract：:NADP(H) binding is essential for fast electron transfer through the flavoprotein domain of the fusion protein P450BM3. Here we characterize the interaction of NADP(H) with the oxidized and partially reduced enzyme and the effect of this interaction on the redox properties of flavin cofactors and electron transfer. Mea...

journal_title：Biochemistry

authors： Murataliev MB,Feyereisen R

更新日期：2000-10-17 00:00:00

abstract：:The crystal structure of the particulate methane monooxygenase (pMMO) from Methylococcus capsulatus (Bath) has been reported recently [Lieberman, R. L., and Rosenzweig, A. C. (2005) Crystal structure of a membrane-bound metalloenzyme that catalyses the biological oxidation of methane, Nature 434, 177-182]. Subsequent ...

journal_title：Biochemistry

authors： Yu SS,Ji CZ,Wu YP,Lee TL,Lai CH,Lin SC,Yang ZL,Wang VC,Chen KH,Chan SI

更新日期：2007-12-04 00:00:00

abstract：:In an attempt to understand the multifunctional involvement of beta(2)-glycoprotein I (beta(2)GPI) in autoimmune diseases, thrombosis, atherosclerosis, and inflammatory processes, substantial interest is focused on the interaction of beta(2)GPI with negatively charged ligands, in particular, with acidic phospholipids....

journal_title：Biochemistry

authors： Hammel M,Schwarzenbacher R,Gries A,Kostner GM,Laggner P,Prassl R

更新日期：2001-11-27 00:00:00

abstract：:Site-directed mutagenesis has been used to construct three recombinant mutant hemoglobins (rHbs), rHb(beta L105W), rHb(alpha D94A/betaL105W), and rHb(alpha D94A). rHb(beta L105W) is designed to form a new hydrogen bond from beta 105Trp to alpha 94Asp in the alpha(1)beta(2) subunit interface to lower the oxygen binding...

journal_title：Biochemistry

authors： Fang TY,Simplaceanu V,Tsai CH,Ho NT,Ho C

更新日期：2000-11-14 00:00:00

abstract：:Chemical modification of some trifunctional amino acid residues in toxins I, II, and III of the scorpion Androctonus australis Hector have been performed. The results indicate: (1) Reduction and methylation of one disulfide bridge destroy toxic activity of toxin II. (2) The only tryptophan residue of toxin II (positio...

journal_title：Biochemistry

authors： Habersetzer-Rochat C,Sampieri F

更新日期：1976-06-01 00:00:00

abstract：:Site-specific mutagenesis has been used to create two mutant versions of aspartate transcarbamoylase. Arg-167 and Gln-231, both previously identified as interacting with the portion of the bisubstrate analogue N-(phosphonoacetyl)-L-aspartate (PALA) that corresponds to aspartate [Krause, K. L., Voltz, K. W., & Lipscomb...

journal_title：Biochemistry

authors： Stebbins JW,Zhang Y,Kantrowitz ER

更新日期：1990-04-24 00:00:00

abstract：:The flavin reductase (FRED) and isobutylamine N-hydroxylase (IBAH) from Streptomyces viridifaciens constitute a two-component, flavin-dependent monooxygenase system that catalyzes the first step in valanimycin biosynthesis. FRED is an oxidoreductase that provides the reduced flavin to IBAH, which then catalyzes the hy...

journal_title：Biochemistry

authors： Li H,Forson B,Eckshtain-Levi M,Valentino H,Martín Del Campo JS,Tanner JJ,Sobrado P

更新日期：2021-01-12 00:00:00

abstract：:CD4 is expressed on the surface of specific leukocytes where it plays a key role in the activation of immunostimulatory T-cells and acts as a primary receptor for HIV-1 entry. CD4 has four ecto-domains (D1-D4) of which D1, D2, and D4 contain disulfide bonds. Although disulfide bonds commonly serve structural or cataly...

journal_title：Biochemistry

authors： Owen GR,Channell JA,Forsyth VT,Haertlein M,Mitchell EP,Capovilla A,Papathanasopoulos M,Cerutti NM

更新日期：2016-04-19 00:00:00

abstract：:Steady-state kinetic parameters were determined for the human leukocyte elastase catalyzed hydrolysis of a series of peptide-based thiobenzyl esters and p-nitroanilides. The peptide units are MeOSuc-Val, MeOSuc-Alan-Pro-Val (n = 0-2), and MeOSuc-Alan-Pro-Ala (n = 1 or 2). The results of this study suggest five importa...

journal_title：Biochemistry

authors： Stein RL,Strimpler AM,Hori H,Powers JC

更新日期：1987-03-10 00:00:00

abstract：:The flavoprotein nitroalkane oxidase catalyzes the oxidation of primary and secondary nitroalkanes to aldehydes and ketones, respectively, transferring electrons to oxygen to form hydrogen peroxide. The steady-state kinetic mechanism of the active flavin adenine dinucleotide-(FAD-) containing form of the enzyme has be...

journal_title：Biochemistry

authors： Gadda G,Fitzpatrick PF

更新日期：2000-02-15 00:00:00

abstract：:Within recent years it has become apparent that protein glycosylation is not limited to eukaryotes. Indeed, in Campylobacter jejuni, a Gram-negative bacterium, more than 60 of its proteins are known to be glycosylated. One of the sugars found in such glycosylated proteins is 2,4-diacetamido-2,4,6-trideoxy-α-d-glucopyr...

journal_title：Biochemistry

authors： Riegert AS,Thoden JB,Schoenhofen IC,Watson DC,Young NM,Tipton PA,Holden HM

更新日期：2017-11-14 00:00:00

abstract：:Human DJ-1, a disease-associated protein that protects cells from oxidative stress, contains an oxidation-sensitive cysteine (C106) that is essential for its cytoprotective activity. The origin of C106 reactivity is obscure, due in part to the absence of an experimentally determined p K a value for this residue. We ha...

journal_title：Biochemistry

authors： Witt AC,Lakshminarasimhan M,Remington BC,Hasim S,Pozharski E,Wilson MA

更新日期：2008-07-15 00:00:00

abstract：:Protein engineering to alter recognition underlying ligand binding and activity has enormous potential. Here, ligand binding for Escherichia coli phosphoenolpyruvate carboxykinase (PEPCK), which converts oxaloacetate into CO2 and phosphoenolpyruvate as the first committed step in gluconeogenesis, was engineered to acc...

journal_title：Biochemistry

authors： Tang HYH,Shin DS,Hura GL,Yang Y,Hu X,Lightstone FC,McGee MD,Padgett HS,Yannone SM,Tainer JA

更新日期：2018-12-04 00:00:00

abstract：:Casein kinase II is a widely distributed protein serine/threonine kinase. The holoenzyme appears to be a tetramer, containing two alpha or alpha' subunits (or one of each) and two beta subunits. Complementary DNA clones encoding the subunits of casein kinase II were isolated from a human T-cell lambda gt10 library usi...

journal_title：Biochemistry

authors： Lozeman FJ,Litchfield DW,Piening C,Takio K,Walsh KA,Krebs EG

更新日期：1990-09-11 00:00:00

abstract：:Activation of washed human platelets initiated with alpha-thrombin, SFLLRN, or AYPGKF invariably results in the generation of PAR-1-(1-41) and PAR-4-(1-47). PAR-1-(1-41) and PAR-4-(1-47) are amino-terminal peptides generated when PAR-1 and -4 are cleaved in their first extracellular domains after R(41) and R(47), resp...

journal_title：Biochemistry

authors： Ofosu FA,Dewar L,Song Y,Cedrone AC,Hortelano G,Craven SJ

更新日期：2009-02-24 00:00:00

abstract：:Most duplex DNAs that are in the "B" conformation are not immunogenic. One important exception is poly(dG) X poly(dC), which produces a good immune response even though, by many criteria, it adopts a conventional right-handed helix. In order to investigate what features are being recognized, monoclonal antibodies were...

journal_title：Biochemistry

authors： Lee JS,Woodsworth ML,Latimer LJ

更新日期：1984-07-03 00:00:00

abstract：:The X-ray structures of neuronal nitric oxide synthase (nNOS) with N(omega)-hydroxy-l-arginine (l-NHA) and CO (or NO) bound have been determined at 1.91-2.2 A resolution. Microspectrophotometric techniques confirmed reduced redox state and the status of diatomic ligand complexes during X-ray diffraction data collectio...

journal_title：Biochemistry

authors： Doukov T,Li H,Soltis M,Poulos TL

更新日期：2009-11-03 00:00:00

abstract：:Bovine brain adenylate cyclase was solubilized with 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate (CHAPS), sodium cholate, sodium deoxycholate, or these detergents plus (NH4)2SO4. The specific activity of the extract obtained with 13 mM CHAPS alone was several times those of the other detergent extracts wi...

journal_title：Biochemistry

authors： Bitonti AJ,Moss J,Hjelmeland L,Vaughan M

更新日期：1982-07-20 00:00:00

abstract：:The three-dimensional structure of the complex between calmodulin (CaM) and a peptide corresponding to the N-terminal portion of the CaM-binding domain of the plasma membrane calcium pump, the peptide C20W, has been solved by heteronuclear three-dimensional nuclear magnetic resonance (NMR) spectroscopy. The structure ...

journal_title：Biochemistry

authors： Elshorst B,Hennig M,Försterling H,Diener A,Maurer M,Schulte P,Schwalbe H,Griesinger C,Krebs J,Schmid H,Vorherr T,Carafoli E

更新日期：1999-09-21 00:00:00

abstract：:The hinge domain encompasses amino acids 51-60 of lactose repressor (LacI) and plays an important role in its regulatory interaction with operator DNA. This segment makes both hinge-DNA and hinge-hinge' contacts that are critical to DNA binding. Furthermore, this small region serves as a central element in communicati...

journal_title：Biochemistry

authors： Falcon CM,Matthews KS

更新日期：2001-12-25 00:00:00