Calcium-promoted fusion of isolated chromaffin granules detected by resonance energy transfer between labeled lipids embedded in the membrane bilayer.

abstract：:The ability of the low molecular weight lung surfactant-associated proteins, SP-B and SP-C, to alter the thermotropic properties of synthetic multilamellar vesicles was tested using differential scanning calorimetry (DSC). The presence of either SP-B or SP-C in dipalmitoylphosphatidylcholine (DPPC) or dipalmitoylphosp...

journal_title：Biochemistry

authors： Shiffer K,Hawgood S,Haagsman HP,Benson B,Clements JA,Goerke J

更新日期：1993-01-19 00:00:00

abstract：:The effect of ionic strength, temperature, and divalent cations on the association of myosin with actin was determined in the ultracentrifuge using scanning absorption optics. The association constant (Ka) for the binding of heavy meromyosin (HmM) to F-actin was 1 X 10(7) M-1 at 20 degrees C, in 0.10 M KCl, 0.01 M imi...

journal_title：Biochemistry

authors： Margossian SS,Lowey S

更新日期：1978-12-12 00:00:00

abstract：:Escherichia coli mannitol specific EII in membrane vesicles can be inhibited by the action of the oxidizable substrate-reduced phenazine methosulfate (PMS) in a manner similar to E. coli enzyme IIGlc [Robillard, G. T., & Konings, W. (1981) Biochemistry 20, 5025-5032]. The fact that reduced PMS and various oxidizing ag...

journal_title：Biochemistry

authors： Roossien FF,Robillard GT

更新日期：1984-01-17 00:00:00

abstract：:An improved procedure for the preparation of cobalt-cytochrome c has been developed. Various factors influencing the cobalt insertion process are discussed. The optical spectra of cobalt-cytochrome c suggest a six-coordinated species. The spectral shifts occurring with oxidation-reduction are compared with those obser...

journal_title：Biochemistry

authors： Dickinson LC,Chien JC

更新日期：1975-08-12 00:00:00

abstract：:The effects of urea on apomyoglobin solubility have been investigated. Apomyoglobin precipitation was found to be a thermodynamically reversible process independent of the pathway of aggregation. A liquid-solid phase diagram was constructed for the precipitation of apomyoglobin as a function of urea and protein concen...

journal_title：Biochemistry

authors： De Young LR,Dill KA,Fink AL

更新日期：1993-04-20 00:00:00

abstract：:RNA helicase DHX33 has been shown to participate in a variety of cellular activities, including ribosome biogenesis, protein translation, and gene transcription. We and others further discovered that DHX33 is strongly expressed in several types of human cancers and plays important roles in promoting cancer cell prolif...

journal_title：Biochemistry

authors： Wang X,Ge W,Zhang Y

更新日期：2019-01-29 00:00:00

abstract：:Stopped-flow fluorescence experiments have been carried out to study the steady-state kinetics of hydrolysis of Leu-Gly-NHNH-Dns [Dns = 5-(dimethylamino)naphthalene-1-sulfonyl] by porcine kidney cytosol leucine aminopeptidase (LAP) in 50% v/v methanol/buffer solution at ambient temperature and the pre-steady-state kin...

journal_title：Biochemistry

authors： Lin WY,Lin SH,Morris RJ,Van Wart HE

更新日期：1988-07-12 00:00:00

abstract：:Heparin potentiates the mitogenic activity of FGF-1 by increasing the affinity for its receptor and by extending its biological half-life. During the course of labeling human FGF-1 with Na(125)I and chloramine T, it was observed that the protein lost its ability to bind to heparin. In contrast, bovine FGF-1 retained i...

journal_title：Biochemistry

authors： Patrie KM,Botelho MJ,Franklin K,Chiu IM

更新日期：1999-07-20 00:00:00

abstract：:Coenzyme A (CoA)-transferases catalyze transthioesterification reactions involving acyl-CoA substrates, using an active-site carboxylate to form covalent acyl anhydride and CoA thioester adducts. Mechanistic studies of class I CoA-transferases suggested that acyl-CoA binding energy is used to accelerate rate-limiting ...

journal_title：Biochemistry

authors： Mullins EA,Kappock TJ

更新日期：2012-10-23 00:00:00

abstract：:Life span regulation and inhibition of gene silencing in yeast have been linked to nicotinamide effects on Sir2 enzymes. The Sir2 enzymes are NAD(+)-dependent protein deacetylases that influence gene expression by forming deacetylated proteins, nicotinamide and 2'-O-acetyl-ADPR. Nicotinamide is a base-exchange substra...

journal_title：Biochemistry

authors： Sauve AA,Schramm VL

更新日期：2003-08-12 00:00:00

abstract：:Listeriolysin O (LLO) is the most important virulence factor of the intracellular pathogen Listeria monocytogenes. Its main task is to enable escape of bacteria from the phagosomal vacuole into the cytoplasm. LLO belongs to the cholesterol-dependent cytolysin (CDC) family but differs from other members, as it exhibits...

journal_title：Biochemistry

authors： Bavdek A,Gekara NO,Priselac D,Gutiérrez Aguirre I,Darji A,Chakraborty T,Macek P,Lakey JH,Weiss S,Anderluh G

更新日期：2007-04-10 00:00:00

abstract：:Aminoimidazole riobnucleotide carboxylase, the sixth step in the purine biosynthetic pathway, catalyzes the conversion of aminoimidazole ribonucleotide (AIR) to carboxyaminoimidazole ribonucleotide (CAIR). The gene products of the purE and purK genes (PurE and PurK, respectively) thought to be responsible for this act...

journal_title：Biochemistry

authors： Meyer E,Leonard NJ,Bhat B,Stubbe J,Smith JM

更新日期：1992-06-02 00:00:00

abstract：:Anatoxin-a and homoanatoxin-a are two potent cyanobacterial neurotoxins. We recently reported the identification of the gene cluster responsible for the biosynthesis of these toxins in cyanobacteria and proposed a biosynthetic scheme starting from L-proline and involving three polyketide synthases for which the starte...

journal_title：Biochemistry

authors： Méjean A,Mann S,Vassiliadis G,Lombard B,Loew D,Ploux O

更新日期：2010-01-12 00:00:00

abstract：:The interaction of a nitroxide spin-labeled derivative of ouabain with sheep kidney Na,K-ATPase and the motional behavior of the ouabain spin label-Na,K-ATPase complex have been studied by means of electron paramagnetic resonance (EPR) and saturation-transfer EPR (ST-EPR). Spin-labeled ouabain binds with high affinity...

journal_title：Biochemistry

authors： Mahaney JE,Grisham CM

更新日期：1992-02-25 00:00:00

abstract：:In order to evaluate the contributions that histidine residues might play both in the catalytic activities of endonuclease V and in binding to nontarget DNA, the technique of oligonucleotide site directed mutagenesis was used to create mutations at each of the four histidine residues in the endonuclease V gene. Althou...

journal_title：Biochemistry

authors： Augustine ML,Hamilton RW,Dodson ML,Lloyd RS

更新日期：1991-08-13 00:00:00

abstract：:The malaria parasite pigment, hemozoin, is a crystal of ferriprotoporphyrin IX (FP-Fe(III)), a product of hemoglobin digestion. Hemozoin formation is essential for FP-Fe(III) detoxification in the parasite; it is the main target of quinoline antimalarials and can modulate immune and inflammation responses. To gain fur...

journal_title：Biochemistry

authors： Klonis N,Dilanian R,Hanssen E,Darmanin C,Streltsov V,Deed S,Quiney H,Tilley L

更新日期：2010-08-10 00:00:00

abstract：:DNA-protein cross-links (DPCs) in nucleosome core particles (NCPs), the fundamental building block of chromatin, arise during times of cellular oxidative stress. These lesions are expected to be detrimental to the cell due to interference with processes like chromatin remodeling, transcription, DNA replication, and ep...

journal_title：Biochemistry

authors： Bjorklund CC,Davis WB

更新日期：2007-09-25 00:00:00

abstract：:The 270-MHz 1H nuclear magnetic resonance spectra of the apo, copper(I)-zinc(II), and copper(II)-zinc(II) forms of bovine erythrocyte superoxide dismutase (EC 1.15.1.1) are reported, and assignments of resonances to ten amino acid residues are proposed. The data require that at least four and probably six histidine re...

journal_title：Biochemistry

authors： Cass AE,Hill AO,Smith BE,Bannister JV,Bannister WH

更新日期：1977-07-12 00:00:00

abstract：:RNA repeat expansions cause a host of incurable, genetically defined diseases. The most common class of RNA repeats consists of trinucleotide repeats. These long, repeating transcripts fold into hairpins containing 1 × 1 internal loops that can mediate disease via a variety of mechanism(s) in which RNA is the central ...

journal_title：Biochemistry

authors： Chen JL,VanEtten DM,Fountain MA,Yildirim I,Disney MD

更新日期：2017-07-11 00:00:00

abstract：:The dynamic behavior of the rRNA A-site plays an important functional role. We have employed femtosecond time-resolved spectroscopy to investigate the nature of the conformational dynamics. In the drug-free state, the A-site samples multiple distinct conformations. Drug binding shifts the population distribution in a ...

journal_title：Biochemistry

authors： Lu J,Zhao L,Xia A,Xia T,Qi X

更新日期：2013-03-12 00:00:00

abstract：:Enzyme-substrate contacts in the hydrolysis of ester substrates by the cysteine protease papain were investigated by systematically altering backbone hydrogen-bonding and side-chain hydrophobic contacts in the substrate and determining each substrate's kinetic constants. The observed specificity energies [defined as d...

journal_title：Biochemistry

authors： Berti PJ,Faerman CH,Storer AC

更新日期：1991-02-05 00:00:00

abstract：:C-type natriuretic peptide (CNP) binds the guanylyl cyclase-linked natriuretic peptide receptor B (NPR-B) and stimulates marked elevations of the intracellular signaling molecule, cGMP. Here, the essential role of phosphorylation in the hormonal activation and deactivation of this receptor is described. Exposure of NI...

journal_title：Biochemistry

authors： Potter LR

更新日期：1998-02-24 00:00:00

abstract：:The cyclic decapeptide antibiotic tyrocidine A was studied by two relatively new methods, viz., correlation proton magnetic resonance (pmr) spectroscopy and double-resonance difference pmr spectroscopy. The correlation method of spectral accumulation provided pmr spectra of good resolution, and in addition the signal-...

journal_title：Biochemistry

authors： Gibbons WA,Beyer CF,Dadok J,Sprecher RF,Wyssbrod HR

更新日期：1975-01-28 00:00:00

abstract：:The effects of an inhibitory monoclonal antibody (mAb) raised against the beta subunit of the Escherichia coli RNA polymerase were determined on the kinetics and structural interactions during formation of the open promoter complex (RPo). Analysis of the kinetics of abortive initiation on linear and supercoiled templa...

journal_title：Biochemistry

authors： Rockwell P,Krakow JS

更新日期：1988-05-03 00:00:00

abstract：:F-STOP is a microtubule-associated protein that stabilizes microtubules in a calmodulin (CaM)-dependent manner. All members of the stable tubule only polypeptide (STOP) family have a central domain that contains nearly identical multiple repeats, and a CaM binding motif is present in multiple copies within this domain...

journal_title：Biochemistry

authors： Bouvier D,Vanhaverbeke C,Simorre JP,Arlaud GJ,Bally I,Forge V,Margolis RL,Gans P,Kleman JP

更新日期：2003-10-07 00:00:00

abstract：:The first epidermal growth factor-like domain (EGF-1) from blood coagulation factor VII (FVII) contains two unusual O-linked glycosylation sites at Ser-52 and Ser-60. We report here a detailed study of the effect of O-fucosylation at Ser-60 on the structure of FVII EGF-1, its Ca2+-binding affinity, and its interaction...

journal_title：Biochemistry

authors： Kao YH,Lee GF,Wang Y,Starovasnik MA,Kelley RF,Spellman MW,Lerner L

更新日期：1999-06-01 00:00:00

abstract：:Six rhodopsin mutants containing disulfide cross-links between different cytoplasmic regions were prepared: disulfide bond 1, between Cys65 (interhelical loop I-II) and Cys316 (end of helix VII); disulfide bond 2, between Cys246 (end of helix VI) and Cys312 (end of helix VII); disulfide bond 3, between Cys139 (end of ...

journal_title：Biochemistry

authors： Cai K,Klein-Seetharaman J,Hwa J,Hubbell WL,Khorana HG

更新日期：1999-09-28 00:00:00

abstract：:The specific effect of the binding of IF2 and initiator fMET-tRNA(fMet) on Escherichia coli 16S rRNA has been probed by phosphate alkylation with ethylnitrosourea. The results show that IF2 does not significantly shield portions of 16S rRNA but induces both reductions and enhancements of reactivity scattered in the en...

journal_title：Biochemistry

authors： Wakao H,Romby P,Laalami S,Ebel JP,Ehresmann C,Ehresmann B

更新日期：1990-09-04 00:00:00

abstract：:In cytochrome c, the coordination of the axial Met Sδ atom to the heme Fe atom occurs in one of two distinctly different stereochemical manners, i.e., R and S configurations, depending upon which of the two lone pairs of the Sδ atom is involved in the bond; hence, the Fe-coordinated Sδ atom becomes a chiral center. In...

journal_title：Biochemistry

authors： Tai H,Tonegawa K,Shibata T,Hemmi H,Kobayashi N,Yamamoto Y

更新日期：2013-07-16 00:00:00

abstract：:The effects of pressure, up to 5 kbar, on multilamellar vesicles of 1,2-dipalmitoyl-sn-phosphatidylcholine perdeuterated in the acyl chains (DPPC-d62) were examined by using high-pressure NMR techniques. A deuterium probe was built, and the quadrupole splitting was measured against pressure at various temperatures. Th...

journal_title：Biochemistry

authors： Driscoll DA,Samarasinghe S,Adamy S,Jonas J,Jonas A

更新日期：1991-04-02 00:00:00