Abstract：
:A number of nucleosides related to S-adenosylmethionine were tested for their inhibitory action on three enzymes involved in the biosynthesis of polyamines. The particular objective of the experiments was to determine whether any of the compounds could be used as selective inhibitors of the synthesis of spermine by spermine synthase. None of the nucleosides examined were potent inhibitors of S-adenosylmethionine decarboxylase. 5'-[(3-Aminopropyl)amino]-5'-deoxyadenosine dihydrochloride was quite a strong inhibitor of spermidine synthase (I50 of 7 microM) but was more than an order of magnitude less active than S-adenosyl-1,8-diamino-3-thiooctane, which is a mechanism-based inhibitor of this enzyme. 5'-[(3-Aminopropyl)amino]-5'-deoxyadenosine also inhibited spermine synthase with an I50 of 17 microM, but more selective inhibition of spermine synthase was produced by 9-[6(RS),8-diamino-5,6,7,8-tetradeoxy-beta-D-ribo-octofuranosyl]-9 H-purin-6- amine (I50 of 12 microM) and by dimethyl(5'-adenosyl)sulfonium perchlorate (I50 of 8 microM) since these compounds were much less active against spermidine synthase. Both 9-[6(RS),8-diamino-5,6,7,8-tetradeoxy-beta-D-ribo-octofuranosyl]-9 H-purin-6- amine and dimethyl(5'-adenosyl)sulfonium perchlorate were able to reduce the synthesis of spermine in SV-3T3 cells, but there was a compensatory increase in the concentration of spermidine, and there was no effect on cell growth. These results and those from experiments in which these spermine synthesis inhibitors were combined with inhibitors of spermidine synthase and ornithine decarboxylase indicated that the cells compensated for the inhibition of the aminopropyltransferases by increasing the production of decarboxylated S-adenosylmethionine and putrescine.(ABSTRACT TRUNCATED AT 250 WORDS)
journal_name
Biochemistryjournal_title
Biochemistryauthors
Pegg AE,Coward JK,Talekar RR,Secrist JA 3rddoi
10.1021/bi00362a016subject
Has Abstractpub_date
1986-07-15 00:00:00pages
4091-7issue
14eissn
0006-2960issn
1520-4995journal_volume
25pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract：:Cell-signaling peptides have been extensively used to transport functional molecules across the plasma membrane into living cells. These peptides consist of a hydrophobic sequence and a cationic nuclear localization sequence (NLS). It has been assumed that the hydrophobic region penetrates the hydrophobic lipid bilaye...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi061895g
更新日期：2007-01-30 00:00:00
abstract：:Two sites of ligand interaction in acetylcholinesterase (AChE) were first demonstrated in ligand binding studies and later confirmed by crystallography, site-specific mutagenesis, and molecular modeling: an acylation site at the base of the active site gorge and a peripheral site at its mouth. We recently introduced a...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi9813577
更新日期：1999-01-05 00:00:00
abstract：:Because of the lack of protein turnover in fiber cells of the ocular lens, Aquaporin 0 (AQP0), the most abundant membrane protein in the lens, undergoes extensive post-translational modification with fiber cell age. To map the distribution of modified forms of AQP0 within the lens, normal human lenses ranging in age f...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0496034
更新日期：2004-08-03 00:00:00
abstract：:In eukaryotic organisms, the largely cytosolic copper- and zinc-containing superoxide dismutase (Cu/Zn SOD) enzyme represents a key defense against reactive oxygen toxicity. Although much is known about the biology of this enzyme under aerobic conditions, less is understood regarding the effects of low oxygen levels o...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi201353y
更新日期：2012-01-17 00:00:00
abstract：:The regulatory (R) subunit of cAMP-dependent protein kinase (cAPK) is a multidomain protein with two tandem cAMP-binding domains, A and B. The importance of cAMP binding on the stability of the R subunit was probed by intrinsic fluorescence and circular dichroism (CD) in the presence and absence of urea. Several mutan...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi001563q
更新日期：2000-12-12 00:00:00
abstract：:The solution of a primary 16S rRNA-binding ribosomal protein, S17, was investigated by two- and three-dimensional homonuclear and heteronuclear magnetic resonance spectroscopy. Almost complete chemical shift assignments for the 1H, 15N, and 13C resonances have been obtained. The NMR data have been rigorously analyzed ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi951062i
更新日期：1996-03-05 00:00:00
abstract：:The chemical and enzymatic syntheses of bacteriophage lambda pseudo operator DNA are described. The 17 base-paired duplex contains the DNA which has been proposed as the binding site for cI repressor protein. The synthetic duplex is twofold symmetric and represents the best possible nucleotide summation of the six pro...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00638a013
更新日期：1977-09-20 00:00:00
abstract：:Nitrobenzamido ligands linked to the DNA intercalator 9-aminoacridine via poly(methylene) chains induce single-strand nicks in DNA upon irradiation with long-wavelength ultraviolet light (lambda greater than or equal to 300 nm). Optimal photocleavage activity was found for the reagent 9-[[6-(4-nitrobenzamido)hexyl]ami...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00417a021
更新日期：1988-08-23 00:00:00
abstract：:Coiled coils are simple models for studying the association of two polypeptide chains to form a folded protein. Previous work has shown that the folding of a coiled coil can be described by a two-state transition between two unfolded monomeric peptide chains and a folded coiled coil dimer. Here we report the thermodyn...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi002161l
更新日期：2001-03-27 00:00:00
abstract：:Distance constraints from two-dimensional NMR cross-relaxation data are used to derive a three-dimensional structure for acyl carrier protein from Escherichia coli. Several approaches to structure determination are explored. The most successful proves to be an approach that combines the early stages of a distance geom...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00416a046
更新日期：1988-08-09 00:00:00
abstract：:A dimeric bis-benzimidazole molecule has been designed by computer modeling to bind to a DNA sequence via the DNA minor groove that covers a complete turn of B-DNA. A series of bis-benzimidazole dimers incorporating a -O-(CH(2))(n)()-X-CH(2))(n)()-O- linker, with n = 2 or 3 and X = O or N(+)H(Me), were screened for th...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi026926w
更新日期：2003-05-27 00:00:00
abstract：:Mitochondria isolated from coenzyme Q deficient yeast cells had no detectable NADH:cytochrome c reductase or succinate:cytochrome c reductase but had comparable amounts of cytochromes b and c1 as wild-type mitochondria. Addition of succinate to the mutant mitochondria resulted in a slight reduction of cytochrome b; ho...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00372a029
更新日期：1986-12-02 00:00:00
abstract：:The interaction of heparan sulfate with different ligand proteins depends on the precise location of O-sulfate groups in the polysaccharide chain. We have previously shown that overexpression in human kidney 293 cells of a mouse mastocytoma 2-O-sulfotransferase (2-OST), previously thought to catalyze the transfer of s...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi002926p
更新日期：2001-05-08 00:00:00
abstract：:The complete amino acid sequence of the major component myoglobin from the dwarf sperm whale, Kogia simus, was determined by specific cleavage of the protein to obtain large peptides which are readily degraded by the automatic sequenator. Three easily separable peptides were obtained by cleaving the protein at its two...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00624a010
更新日期：1977-03-08 00:00:00
abstract：:The binding of 125I-labeled ovine follitropin (oFSH) and 125I-labeled deglycosylated ovine follitropin (DG-oFSH) to porcine granulosa cell membranes was studied at equilibrium and nonequilibrium binding conditions and statistically analyzed. Saturation and competition binding experiments revealed homogeneity in the po...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00386a059
更新日期：1987-06-16 00:00:00
abstract：:Excitation energy transfer studies were carried out to determine the distance between the adenosinetriphosphatase (ATPase) site and a unique "fast-reacting" sulfhydryl (referred to as SH1) in myosin subfragment 1. The fluorescent moiety of the probe N-(iodoacetyl)-N'-(5-sulfo-1-naphthyl)ethylene-diamine was used as th...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00520a035
更新日期：1981-08-18 00:00:00
abstract：:In the reaction center (RC) of the photosynthetic bacterium Rhodobacter sphaeroides, two ubiquinone molecules, QA and QB, play a pivotal role in the conversion of light energy into chemical free energy by coupling electron transfer to proton uptake. In native RCs, the transfer of an electron from QA to QB takes place ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi700297b
更新日期：2007-04-17 00:00:00
abstract：:Alginate is a heteropolysaccharide that consists of β-D-mannuronate (M) and α-L-guluronate (G). The Gram-negative bacterium Sphingomonas sp. A1 directly incorporates alginate into the cytoplasm through the periplasmic solute-binding protein (AlgQ1 and AlgQ2)-dependent ABC transporter (AlgM1-AlgM2/AlgS-AlgS). Two bindi...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi300194f
更新日期：2012-05-01 00:00:00
abstract：:Binding studies of the interaction of immobilized 1alpha- and 17alpha-aminoalkyl derivatives of 5alpha-dihydrotestosterone (DHT) with purified N-deglycosylated homodimeric human sex hormone-binding globulin (SHBG) were performed using a surface plasmon resonance biosensor. These 1alpha- and 17alpha-derivatives with sp...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi035269k
更新日期：2003-11-25 00:00:00
abstract：:The class D β-lactamase OXA-143 has been described as an efficient penicillinase, oxacillinase, and carbapenemase. The D224A variant, known as OXA-231, was described in 2012 as exhibiting less activity toward imipenem and increased oxacillinase activity. Additionally, the P227S mutation was reported as a case of conve...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.9b00365
更新日期：2019-08-27 00:00:00
abstract：:Studying the evolution of catalytically promiscuous enzymes like those from the N-succinylamino acid racemase/ o-succinylbenzoate synthase (NSAR/OSBS) subfamily can reveal mechanisms by which new functions evolve. Some enzymes in this subfamily have only OSBS activity, while others catalyze OSBS and NSAR reactions. We...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.8b00088
更新日期：2018-07-03 00:00:00
abstract：:In a previously determined minimal kinetic scheme for DNA polymerization catalyzed by the Klenow fragment (KF) of Escherichia coli DNA polymerase I, a nonchemical step that interconverted the KF'.DNAn+1.PPi and KF.DNAn+1PPi complexes was not observed in correct incorporation [Kuchta, R. D., Mizrahi, V., Benkovic, P.A....
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00234a002
更新日期：1991-05-21 00:00:00
abstract：:In bee venom phospholipase A2, histidine-34 probably functions as a Brønsted base to deprotonate the attacking water. Aspartate-64 and tyrosine-87 form a hydrogen bonding network with histidine-34. We have prepared mutants at these positions and studied their kinetic properties. The mutant in which histidine-34 is cha...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi9528412
更新日期：1996-04-09 00:00:00
abstract：:The human ferritin L-chain cDNA was cloned into a vector for overproduction in Escherichia coli, under the regulation of a lambda promoter. The plasmid obtained contains the full L-chain coding region modified at the first two codons. It is able to direct the synthesis of the L-chain which can constitute up to 15% of ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00438a040
更新日期：1989-06-13 00:00:00
abstract：:Poly(ethylene glycol) (PEG) of average molecular weight 8000 was used to mediate the fusion of large unilamellar vesicles composed of dipalmitoylphosphatidylcholine. Fusion was monitored by fluorescence assays of lipid mixing and aqueous contents mixing. The extent of lipid mixing, as monitored by DPHpPC fluorescence ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00369a053
更新日期：1986-10-21 00:00:00
abstract：:Retroviruses contain a dimeric RNA consisting of two identical molecules of genomic RNA. The interaction between the two monomers is thought to occur near their 5'ends. We previously identified a region upstream from the splice donor site, comprising an autocomplementary sequence, responsible for the formation of dime...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi952822s
更新日期：1996-04-16 00:00:00
abstract：:Arginine vasopressin (AVP) is a nonapeptide long known as an endocrine and paracrine regulator of important systemic functions, namely, vasoconstriction, gluconeogenesis, corticosteroidogenesis, and excretion of water and urea. Here we report, for the first time, that AVP specifically inhibits expression of the cyclin...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi026807g
更新日期：2003-02-25 00:00:00
abstract：:Photoaffinity labeling of the nicotinic acetylcholine receptor from Torpedo marmorata electric tissue was performed in the presence of cholinergic effectors in the millisecond to second time range by a combination of a stopped-flow apparatus and a high-energy pulse laser. The label applied was [3H]triphenylmethylphosp...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00307a029
更新日期：1984-06-05 00:00:00
abstract：:Coiled coils (CCs) are among the best-understood protein folds. Nonetheless, there are gaps in our knowledge of CCs. Notably, CCs are likely to be structurally more dynamic than often considered. Here, we explore this in an abundant class of CCs, parallel dimers, focusing on polar asparagine (Asn) residues in the hydr...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.7b00848
更新日期：2017-12-19 00:00:00
abstract：:The energy derived from optimized van der Waals interactions in closely packed, folded proteins has been proposed to be of similar energetic magnitude to hydrophobicity in stabilizing the native state. If packing is this energetically important, it should influence the evolution of protein core sequences. To test this...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi011776v
更新日期：2001-12-18 00:00:00
