Solution structure of prokaryotic ribosomal protein S17 by high-resolution NMR spectroscopy.

abstract：:Glutamate racemase, MurI, catalyzes the interconversion of glutamate enantiomers in a cofactor-independent fashion and provides bacteria with a source of D-Glu for use in peptidoglycan biosynthesis. The enzyme uses a "two-base" mechanism involving a deprotonation of the substrate at the alpha-position to form an anion...

journal_title：Biochemistry

authors： Glavas S,Tanner ME

更新日期：2001-05-29 00:00:00

abstract：:Gp is a major GTP-binding protein of human placenta and platelets [Evans, T., Brown, M. L., Fraser, E. D., & Northup, J. K. (1986) J. Biol. Chem. 261, 7052-7059]. High-affinity guanine nucleotide binding is associated with a polypeptide migrating identically with H-ras on SDS-PAGE. We have characterized the interactio...

journal_title：Biochemistry

authors： Tamir A,Fawzi AB,Northup JK

更新日期：1990-07-31 00:00:00

abstract：:Five gangliosides, accounting for over 95% of the total ganglioside fraction, were isolated from bovine adrenal medulla by preparative thin-layer chromatography and the carbohydrate structures determined by a combination of periodate oxidation and permethylation techniques. Partially methylated alditol acetates were g...

journal_title：Biochemistry

authors： Price H,Kundu S,Ledeen R

更新日期：1975-04-08 00:00:00

abstract：:Abortive transcription initiation can be rate-limiting for promoter escape and therefore represents a barrier to productive gene expression. The mechanism for abortive initiation is unknown, but the amount of abortive transcript is known to vary with the composition of the initial transcribed sequence (ITS). Here, we ...

journal_title：Biochemistry

authors： Skancke J,Bar N,Kuiper M,Hsu LM

更新日期：2015-07-21 00:00:00

abstract：:A conformational change in the DNA plasmid ColE1 appears to occur upon specific binding of the restriction endonuclease EcoRI. Enzyme association alters the chiral discrimination found in binding metallointercalators to DNA sites. The complexes tris(1,10-phenanthroline)ruthenium(II), Ru(phen)3(2+), tris(4,7-diphenyl-1...

journal_title：Biochemistry

authors： Barton JK,Paranawithana SR

更新日期：1986-04-22 00:00:00

abstract：:In order to quantitate the contributions of the polar, uncharged amino acids to the stability of the native state of staphylococcal nuclease, each of the 13 alanines, 9 glycines, 9 threonines, 6 prolines, 6 glutamines, 6 asparagines, and 3 serines was substituted, either with both alanine and glycine or with 1 of thes...

journal_title：Biochemistry

authors： Green SM,Meeker AK,Shortle D

更新日期：1992-06-30 00:00:00

abstract：:The chromatin-associated high-mobility group (HMG) proteins of the plant HMGB family are characterized by a central HMG-box domain that is flanked by a basic N-terminal and an acidic C-terminal domain. By functional interaction with certain transcription factors, HMGB proteins contribute to transcriptional regulation....

journal_title：Biochemistry

authors： Grasser M,Christensen JM,Peterhänsel C,Grasser KD

更新日期：2007-05-29 00:00:00

abstract：:One of the key pathological features of Alzheimer's disease is the aggregation of tau protein. We are therefore searching for compounds capable of inhibiting this reaction. On the basis of an initial screen of 200000 compounds [Pickhardt, M., Gazova, Z., von Bergen, M., Khlistunova, I., Wang, Y., Hascher, A., Mandelko...

journal_title：Biochemistry

authors： Pickhardt M,Larbig G,Khlistunova I,Coksezen A,Meyer B,Mandelkow EM,Schmidt B,Mandelkow E

更新日期：2007-09-04 00:00:00

abstract：:Fibrinogen is a ligand for leukocyte integrin alpha(M)beta2 (CD11b/CD18, Mac-1) and mediates adhesion and migration of leukocytes during the immune-inflammatory responses. The binding site for alpha(M)beta2 resides in gammaC, a constituent subdomain in the D-domain of fibrinogen. The sequence gamma383-395 (P2-C) in ga...

journal_title：Biochemistry

authors： Lishko VK,Kudryk B,Yakubenko VP,Yee VC,Ugarova TP

更新日期：2002-10-29 00:00:00

abstract：:Laminins are large multidomain proteins of the extracellular matrix (ECM) with important functions in the development and maintenance of cellular organization and supramolecular structure, in particular in basement membranes. Each molecule is composed of three polypeptide chains, A (300-400 kDa) and B1 and B2 (180-200...

journal_title：Biochemistry

authors： Engel J

更新日期：1992-11-10 00:00:00

abstract：:Endonuclease VIII (Nei) is one of three enzymes in Escherichia coli that are involved in base-excision repair of oxidative damage to DNA. We investigated the substrate specificity and excision kinetics of this DNA glycosylase for bases in DNA that have been damaged by free radicals. Two different DNA substrates were p...

journal_title：Biochemistry

authors： Dizdaroglu M,Burgess SM,Jaruga P,Hazra TK,Rodriguez H,Lloyd RS

更新日期：2001-10-09 00:00:00

abstract：:Conformational selection and induced fit are two well-known mechanisms of allosteric protein-ligand interaction. Some proteins, like ubiquitin, have recently been found to exist in multiple conformations at equilibrium, suggesting that the conformational selection may be a general mechanism of interaction, in particul...

journal_title：Biochemistry

authors： Chi CN,Bach A,Engström A,Wang H,Strømgaard K,Gianni S,Jemth P

更新日期：2009-08-04 00:00:00

abstract：:Perioperative bleeding is a cause of major blood loss and is associated with increased rates of postoperative morbidity and mortality. To combat this, antifibrinolytic inhibitors of the serine protease plasmin are commonly used to reduce bleeding during surgery. The most effective and previously widely used of these i...

journal_title：Biochemistry

authors： Swedberg JE,Harris JM

更新日期：2011-10-04 00:00:00

abstract：:The traditional approach to studying protein folding involves applying a perturbation, usually denaturant or mutation, and determining the effect upon the free energy of folding, DeltaG0, and the activation free energy, DeltaG(not equal). Data collected as a function of the perturbation can be used to construct rate e...

journal_title：Biochemistry

authors： Song B,Cho JH,Raleigh DP

更新日期：2007-12-11 00:00:00

abstract：:myo-Inositol oxygenase (MIOX) uses iron as its cofactor and dioxygen as its cosubstrate to effect the unique, ring-cleaving, four-electron oxidation of its cyclohexan-(1,2,3,4,5,6-hexa)-ol substrate to d-glucuronate. The nature of the iron cofactor and its interaction with the substrate, myo-inositol (MI), have been p...

journal_title：Biochemistry

authors： Xing G,Hoffart LM,Diao Y,Prabhu KS,Arner RJ,Reddy CC,Krebs C,Bollinger JM Jr

更新日期：2006-05-02 00:00:00

abstract：:Rhodospirillum rubrum ribulose bisphosphate carboxylase contains two high affinity binding sites for pyridoxal phosphate and two catalytic sites per dimer. However, pyridoxal phosphate binding at only one site is sufficient for inactivation of both catalytic sites. In the presence of 20 mM bicarbonate, 10 mM magnesium...

journal_title：Biochemistry

authors： Whitman WB,Tabita FR

更新日期：1978-04-04 00:00:00

abstract：:Efforts to elucidate the nature of the bimolecular interaction of parathyroid hormone (PTH) with its cognate receptor, the PTH receptor type 1 (PTHR1), have relied heavily on benzoylphenylalanine- (Bpa-) based photoaffinity cross-linking. However, given the flexibility, size, and shape of Bpa, the resolution at the PT...

journal_title：Biochemistry

authors： Monaghan P,Thomas BE,Woznica I,Wittelsberger A,Mierke DF,Rosenblatt M

更新日期：2008-06-03 00:00:00

abstract：:Whereas the salt-dependent conformational transition of acid-denatured horse ferricytochrome c at pH 2 is approximated by a two-state mechanism from the acid-unfolded state to the molten globule state [Kataoka, M., Hagihara, Y., Mihara, K., & Goto, Y. (1993) J. Mol. Biol. 229, 591-596], the corresponding transition in...

journal_title：Biochemistry

authors： Goto Y,Hagihara Y,Hamada D,Hoshino M,Nishii I

更新日期：1993-11-09 00:00:00

abstract：:Crystals of the Y195F mutant of cyclodextrin glycosyltransferase from Bacillus circulans strain 251 were subjected to a double soaking procedure, in which they were first soaked in a solution containing the inhibitor acarbose and subsequently in a solution containing maltohexaose. The refined structure of the resultin...

journal_title：Biochemistry

authors： Strokopytov B,Knegtel RM,Penninga D,Rozeboom HJ,Kalk KH,Dijkhuizen L,Dijkstra BW

更新日期：1996-04-02 00:00:00

abstract：:Picosecond transient absorption spectra of rhodopsin and isorhodopsin were measured at room temperature with a double-beam laser spectrophotometer after excitation at 355 nm. Photolysis studies were performed on rhodopsin solubilized in two different detergents (digitonin and 3-[(3-cholamidopropyl)dimethylammonio]-1-p...

journal_title：Biochemistry

authors： Rudzki JE,Peters KS

更新日期：1984-08-14 00:00:00

abstract：:5'-Nucleotidase has been extracted from rat heart and purified to apparent homogeneity. The enzyme is a glycoprotein. Gel electrophoresis in the presence of sodium dodecyl sulfate indicates that the apparent molecular weight of the subunit is 74 000 at several different gel concentrations. Cross-linking of the native ...

journal_title：Biochemistry

authors： Naito Y,Lowenstein JM

更新日期：1981-09-01 00:00:00

abstract：:Alpha2u-globulin is a male rat liver protein under multihormonal control which represents approximately 1% of hepatic protein synthesis. We have measured the rate of transcription of the alpha2u-globulin gene using a nuclear cell-free transcriptional system with mercurated CTP as substrate for the endogenous RNA polym...

journal_title：Biochemistry

authors： Chan KM,Kurtz DT,Feigelson P

更新日期：1978-07-25 00:00:00

abstract：:A Rhodobacter sphaeroides bchD (magnesium chelatase) mutant was studied to determine the properties of its photosystem in the absence of bacteriochlorophyll (BChl). Western blots of reaction center H, M, and L (RC H/M/L) proteins from mutant membranes showed levels of 12% RC H, 32% RC L, and 46% RC M relative to those...

journal_title：Biochemistry

authors： Jaschke PR,Beatty JT

更新日期：2007-10-30 00:00:00

abstract：:Diverse voltage-gated K+ channels are produced by tetramerization of channel alpha subunits that can be encoded by multiple genes. In GH3 pituitary cells, the Kv1.4 and Kv1.5 subunit genes are constitutively expressed, and dexamethasone selectively up-regulates expression of the latter gene. Here we use subunit-specif...

journal_title：Biochemistry

authors： Takimoto K,Levitan ES

更新日期：1996-11-12 00:00:00

abstract：:Aerobic and facultative bacteria and archaea harboring mer loci exhibit resistance to the toxic effects of Hg(II) and organomercurials [RHg(I)]. In broad spectrum resistance, RHg(I) is converted to less toxic Hg(0) in the cytosol by the sequential action of organomercurial lyase (MerB: RHg(I) → RH + Hg(II)) and mercur...

journal_title：Biochemistry

authors： Hong B,Nauss R,Harwood IM,Miller SM

更新日期：2010-09-21 00:00:00

abstract：:Fourier transform infrared difference spectroscopy has been used to obtain information about substrate-induced structural changes of the melibiose permease (MelB) from Escherichia coli reconstituted into liposomes. Binding of the cosubstrate Na(+) gives rise to several peaks in the amide I and II regions of the differ...

journal_title：Biochemistry

authors： León X,Lórenz-Fonfría VA,Lemonnier R,Leblanc G,Padrós E

更新日期：2005-03-08 00:00:00

abstract：:The contribution of ligand dynamics to CYP allosterism has not been considered in detail. On the basis of a previous study, we hypothesized that CYP2A6 and CYP2E1 accommodate multiple xylene ligands. As a result, the intramolecular ( k H/ k D) obs values observed for some xylene isomers are expected to be dependent on...

journal_title：Biochemistry

authors： Harrelson JP,Atkins WM,Nelson SD

更新日期：2008-03-04 00:00:00

abstract：:Decapping is the first committed step in 5'-to-3' RNA decay, and in the cytoplasm of human cells, multiple decapping enzymes regulate the stabilities of distinct subsets of cellular transcripts. However, the complete set of RNAs regulated by any individual decapping enzyme remains incompletely mapped, and no consensus...

journal_title：Biochemistry

authors： Luo Y,Schofield JA,Simon MD,Slavoff SA

更新日期：2020-11-03 00:00:00

abstract：:Previous studies of titratable (Na+ + K+)-ATPase sulfhydryl groups have indicated the presence of one disulfide bond per mole of holoenzyme. This single disulfide cross-link was assigned to the beta subunit on the basis of the difference between the number of titrated "free" sulfhydryl groups and the total number of t...

journal_title：Biochemistry

authors： Miller RP,Farley RA

更新日期：1990-02-13 00:00:00

abstract：:Snake venom contains a number of the hemostatically active C-type lectin-like proteins, which affect the interaction between von Willebrand factor (vWF) and the platelet glycoprotein (GP) Ib or platelet receptor to inhibit/induce platelet activation. Flavocetin-A (FL-A) is a high-molecular mass C-type lectin-like prot...

journal_title：Biochemistry

authors： Fukuda K,Mizuno H,Atoda H,Morita T

更新日期：2000-02-29 00:00:00