当前位置： SCI文献检索 > BIOCHEMISTRY期刊下所有文献 > Importance of the β5-β6 Loop for the Structure, Catalytic Efficiency, and Stability of Carbapenem-Hydrolyzing Class D β-Lactamase Subfamily OXA-143.

Importance of the β5-β6 Loop for the Structure, Catalytic Efficiency, and Stability of Carbapenem-Hydrolyzing Class D β-Lactamase Subfamily OXA-143.

Abstract：

:The class D β-lactamase OXA-143 has been described as an efficient penicillinase, oxacillinase, and carbapenemase. The D224A variant, known as OXA-231, was described in 2012 as exhibiting less activity toward imipenem and increased oxacillinase activity. Additionally, the P227S mutation was reported as a case of convergent evolution for homologous enzymes. To investigate the impact of both mutations (D224A and P227S), we describe in this paper a deep investigation of the enzymatic activities of these three homologues. OXA-143(P227S) presented enhanced catalytic activity against ampicillin, oxacillins, aztreonam, and carbapenems. In addition, OXA-143(P227S) was the only member capable of hydrolyzing ceftazidime. These enhanced activities were due to a combination of a higher affinity (lower Km) and a higher turnover number (higher kcat). We also determined the crystal structure of apo OXA-231. As expected, the structure of this variant is very similar to the published OXA-143 structure, except for the two M223 conformations and the absence of electron density for three solvent-exposed loop segments. Molecular dynamics calculations showed that both mutants experience higher flexibility compared to that of the wild-type form. Therefore, our results illustrate that D224A and P227S act as deleterious and positive mutations, respectively, within the evolutionary path of the OXA-143 subfamily toward a more efficient carbapenemase.

journal_name

Biochemistry

journal_title

Biochemistry

authors

Antunes VU,Llontop EE,Vasconcelos FNDC,López de Los Santos Y,Oliveira RJ,Lincopan N,Farah CS,Doucet N,Mittermaier A,Favaro DC

doi

10.1021/acs.biochem.9b00365

subject

Has Abstract

pub_date

2019-08-27 00:00:00

pages

3604-3616

issue

34

eissn

0006-2960

issn

1520-4995

journal_volume

58

pub_type

杂志文章

相关文献

BIOCHEMISTRY文献大全
  • Gal repressor-operator-HU ternary complex: pathway of repressosome formation.

    abstract：:DNA transaction reactions require formation of nucleoprotein complexes that involve multifaceted DNA-protein and protein-protein interactions. Genetic and biochemical studies suggested that the higher order Gal repressosome structure, which governs the transcription of two tandem galpromoters in Escherichia coli, invo...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi047720t

    authors： Roy S,Dimitriadis EK,Kar S,Geanacopoulos M,Lewis MS,Adhya S

    更新日期：2005-04-12 00:00:00

  • Kinetics of polymerization of a fluoresceinated derivative of complement protein C9 by the membrane-bound complex of complement proteins C5b-8.

    abstract：:The fluorescence self-quenching by energy transfer of FITC-C9, a fluoresceinated derivative of human complement protein C9 [Sims, P.J. (1984) Biochemistry (preceding paper in this issue)], has been used to monitor the kinetics of C9 polymerization induced by the membrane-associated complex of complement proteins C5b-8...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00309a021

    authors： Sims PJ,Wiedmer T

    更新日期：1984-07-03 00:00:00

  • L-cysteine desulfidase: an [4Fe-4S] enzyme isolated from Methanocaldococcus jannaschii that catalyzes the breakdown of L-cysteine into pyruvate, ammonia, and sulfide.

    abstract：:A [4Fe-4S] enzyme that decomposes L-cysteine to hydrogen sulfide, ammonia, and pyruvate has been isolated and characterized from Methanocaldococcus jannaschii. The sequence of the isolated enzyme demonstrated that the protein was the product of the M. jannaschii MJ1025 gene. The protein product of this gene was recomb...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi0484769

    authors： Tchong SI,Xu H,White RH

    更新日期：2005-02-08 00:00:00

  • Mammalian folylpoly-gamma-glutamate synthetase. 1. Purification and general properties of the hog liver enzyme.

    abstract：:Folylpolyglutamate synthetase was purified 30,000-150,000-fold from hog liver. Purification required the use of protease inhibitors, and the protein was purified to homogeneity in two forms. Both forms of the enzyme were monomers of Mr 62,000 and had similar specific activities. The specific activity of the homogeneou...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00376a024

    authors： Cichowicz DJ,Shane B

    更新日期：1987-01-27 00:00:00

  • The polar nature of 7-ketocholesterol determines its location within membrane domains and the kinetics of membrane microsolubilization by apolipoprotein A-I.

    abstract：:7-Ketocholesterol is an oxidized derivative of cholesterol with numerous physiological effects. In model membranes, 7-ketocholesterol and cholesterol were compared by physical measures of bilayer order and polarity, formation of detergent resistant domains (DRM), phase separation, and membrane microsolubilization by a...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi0506425

    authors： Massey JB,Pownall HJ

    更新日期：2005-08-02 00:00:00

  • pH dependence of amylin fibrillization.

    abstract：:In type 2 diabetics, the hormone amylin misfolds into amyloid plaques implicated in the destruction of the pancreatic β-cells that make insulin and amylin. The aggregative misfolding of amylin is pH-dependent, and exposure of the hormone to acidic and basic environments could be physiologically important. Amylin has t...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi401164k

    authors： Jha S,Snell JM,Sheftic SR,Patil SM,Daniels SB,Kolling FW,Alexandrescu AT

    更新日期：2014-01-21 00:00:00

  • The betagamma-crystallin superfamily contains a universal motif for binding calcium.

    abstract：:The betagamma-crystallin superfamily consists of evolutionarily related proteins with domain topology similar to lens beta- and gamma-crystallins, formed from duplicated Greek key motifs. Ca(2+) binding was found in a few betagamma-crystallin members earlier, although its prevalence and diversity as inherent molecular...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi9017076

    authors： Aravind P,Mishra A,Suman SK,Jobby MK,Sankaranarayanan R,Sharma Y

    更新日期：2009-12-29 00:00:00

  • The axial tyrosinate Fe3+ ligand in protocatechuate 3,4-dioxygenase influences substrate binding and product release: evidence for new reaction cycle intermediates.

    abstract：:The essential active site Fe3+ of protocatechuate 3,4-dioxygenase [3, 4-PCD, subunit structure (alphabetaFe3+)12] is bound by axial ligands, Tyr447 (147beta) and His462 (162beta), and equatorial ligands, Tyr408 (108beta), His460 (160beta), and a solvent OH- (Wat827). Recent X-ray crystallographic studies have shown th...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi972047b

    authors： Frazee RW,Orville AM,Dolbeare KB,Yu H,Ohlendorf DH,Lipscomb JD

    更新日期：1998-02-24 00:00:00

  • hnRNP A2 selectively binds the cytoplasmic transport sequence of myelin basic protein mRNA.

    abstract：:Segregation of mRNAs in the cytoplasm of polar cells has been demonstrated for proteins involved in Xenopus and Drosophila oogenesis, and for some proteins in somatic cells. It is assumed that vectorial transport of the messages is generally responsible for this localization. The mRNA encoding the basic protein of cen...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi9800247

    authors： Hoek KS,Kidd GJ,Carson JH,Smith R

    更新日期：1998-05-12 00:00:00

  • Comparison of the crystal and solution structures of calmodulin and troponin C.

    abstract：:X-ray solution scattering data from skeletal muscle troponin C and from calmodulin have been measured. Modeling studies based on the crystal structure coordinates for these proteins show discrepancies between the solution data and the crystal structure that indicate that if the size and shape of the globular domains a...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00403a011

    authors： Heidorn DB,Trewhella J

    更新日期：1988-02-09 00:00:00

  • Probing the mechanism of hamster arylamine N-acetyltransferase 2 acetylation by active site modification, site-directed mutagenesis, and pre-steady state and steady state kinetic studies.

    abstract：:Arylamine N-acetyltransferases (NATs) catalyze an acetyl group transfer from acetyl coenzyme A (AcCoA) to arylamines, hydrazines, and their N-hydroxylated arylamine metabolites. The recently determined three-dimensional structures of prokaryotic NATs have revealed a cysteine protease-like Cys-His-Asp catalytic triad, ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi0497244

    authors： Wang H,Vath GM,Gleason KJ,Hanna PE,Wagner CR

    更新日期：2004-06-29 00:00:00

  • Dehydroquinate synthase: the use of substrate analogues to probe the early steps of the catalyzed reaction.

    abstract：:The early steps of the proposed mechanistic pathway for dehydroquinate synthase have been probed with a series of substrate analogues. These analogues, 3-9, are structurally prohibited from undergoing the beta-elimination of inorganic phosphate that represents the committed step in the conversion of the substrate 3-de...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00445a010

    authors： Bender SL,Widlanski T,Knowles JR

    更新日期：1989-09-19 00:00:00

  • Analysis of substrate access to active sites in bacterial multicomponent monooxygenase hydroxylases: X-ray crystal structure of xenon-pressurized phenol hydroxylase from Pseudomonas sp. OX1.

    abstract：:In all structurally characterized bacterial multicomponent monooxygenase (BMM) hydroxylase proteins, a series of hydrophobic cavities in the α-subunit trace a conserved path from the protein exterior to the carboxylate-bridged diiron active site. This study examines these cavities as a potential route for transport of...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi201248b

    authors： McCormick MS,Lippard SJ

    更新日期：2011-12-27 00:00:00

  • Mapping the bimolecular interface of the parathyroid hormone (PTH)-PTH1 receptor complex: spatial proximity between Lys(27) (of the hormone principal binding domain) and leu(261) (of the first extracellular loop) of the human PTH1 receptor.

    abstract：:In an effort to characterize the bimolecular interface between parathyroid hormone (PTH) and its human receptor PTH1-Rc (hPTH1-Rc), we previously identified two contact sites in the receptor: one for position 1 and another for position 13 (located at the ends of the principal activation domain) in PTH(1-34). The prese...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi000195n

    authors： Greenberg Z,Bisello A,Mierke DF,Rosenblatt M,Chorev M

    更新日期：2000-07-18 00:00:00

  • Characterization of the ligand binding activities of vitronectin: interaction of vitronectin with lipids and identification of the binding domains for various ligands using recombinant domains.

    abstract：:Vitronectin is a multifunctional plasma glycoprotein which may regulate the systems related to protease cascades such as the coagulation, fibrinolysis, and complement systems as well as cell adhesion. Solid-phase assays and affinity chromatography on immobilized glycolipids indicated that vitronectin purified under de...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi972247n

    authors： Yoneda A,Ogawa H,Kojima K,Matsumoto I

    更新日期：1998-05-05 00:00:00

  • Role of proline residues in the structure and function of a membrane transport protein.

    abstract：:By use of site-directed mutagenesis, each prolyl residue in the lac permease of Escherichia coli at positions 28 (putative helix I), 31 (helix I), 61 (helix II), 89 (helix III), 97 (helix III), 123 (helix IV), 192 (putative hydrophilic region 7), 220 (helix VII), 280 (helix VIII), and 327 [helix X; Lolkema, J. S., et ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00219a019

    authors： Consler TG,Tsolas O,Kaback HR

    更新日期：1991-02-05 00:00:00

  • Carbon monoxide binding to human hemoglobin A0.

    abstract：:The carbon monoxide binding curve to human hemoglobin A0 has been measured to high precision in experimental conditions of 600 microM heme, 0.1 M N-(2-hydroxyethyl)piperazine-N'-2-ethanesulfonic acid, 0.1 M NaCl, 10 mM inositol hexaphosphate, 1 mM disodium ethylenediaminetetraacetic acid, pH 6.94, and 25 degrees C. Co...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00394a031

    authors： Di Cera E,Doyle ML,Connelly PR,Gill SJ

    更新日期：1987-10-06 00:00:00

  • Reactivity of fibrinogen and fibrinopeptide A containing fibrinogen fragments with antisera to fibrinopeptide A.

    abstract：:Two antisera used in the radioimmunoassay for human fibrinopeptide A (FPA) which appear to have different immunochemical specificities have been tested for cross-reactivity with fibrinogen and with three fragments of fibrinogen which contain the FPA sequence. These fragments were the three-chain, NH2-terminal disulfid...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00651a004

    authors： Canfield RE,Dean J,Nossel HL,Butler VP Jr,Wilner GD

    更新日期：1976-03-23 00:00:00

  • Pharmacological and functional comparison of the polo-like kinase family: insight into inhibitor and substrate specificity.

    abstract：:PLK1 (polo-like kinase 1) is a key mitotic kinase and a therapeutic target in the treatment of proliferative diseases. Here we investigate the relative substrate specificity and pharmacological relatedness of PLK1, -2, -3, and -4 that together comprise a conserved family of Ser/Thr kinases (PLK family). We report cons...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi7008745

    authors： Johnson EF,Stewart KD,Woods KW,Giranda VL,Luo Y

    更新日期：2007-08-21 00:00:00

  • MAP4 is the in vivo substrate for CDC2 kinase in HeLa cells: identification of an M-phase specific and a cell cycle-independent phosphorylation site in MAP4.

    abstract：:We reported previously that cdc2 kinase decreased the microtubule-stabilizing ability of a major HeLa cell microtubule-associated protein, MAP4, by phosphorylation in vitro [Ookata, K., et al. (1995) J. Cell Biol. 128, 849-862]. An important question raised by this study is whether MAP4 is indeed phosphorylated by cdc...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi971251w

    authors： Ookata K,Hisanaga S,Sugita M,Okuyama A,Murofushi H,Kitazawa H,Chari S,Bulinski JC,Kishimoto T

    更新日期：1997-12-16 00:00:00

  • Mechanism of autophosphorylation of mycobacterial PknB explored by molecular dynamics simulations.

    abstract：:Mycobacterial Ser/Thr kinase, PknB, is essential for the growth of the pathogen. Unphosphorylated PknB is catalytically inactive, and its activation requires autophosphorylation of Thr residues on the activation loop. Autophosphorylation can in principle take place via two distinct mechanisms. Intermolecular trans aut...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi500245v

    authors： Damle NP,Mohanty D

    更新日期：2014-07-22 00:00:00

  • Spectroscopic studies of ascorbate oxidase. Electronic structure of the blue copper sites.

    abstract：:Low-temperature electronic absorption and room temperature circular dichroism (CD) and magnetic circular dichroism (MCD) spectra are reported for ascorbate oxidase. Bands attributable to d-d electronic transitions in the type 1 (blue) coppers (2B2 ground state) have been observed at 5800, 10000, and 12000 cm-1 (1725, ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00510a044

    authors： Dooley DM,Dawson JH,Stephens PJ,Gray HB

    更新日期：1981-03-31 00:00:00

  • Ca(2+)-dependent binding of endonexin (annexin IV) to membranes: analysis of the effects of membrane lipid composition and development of a predictive model for the binding interaction.

    abstract：:Endonexin (annexin IV) is a member of the annexin family of homologous proteins that bind membranes and aggregate vesicles in a calcium-dependent fashion. This study examines the lipid modulation and mechanism of the binding of endonexin to membranes using a fluorescence energy transfer assay to measure bovine endonex...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00196a010

    authors： Junker M,Creutz CE

    更新日期：1994-08-02 00:00:00

  • Crystal structure of an ecotin-collagenase complex suggests a model for recognition and cleavage of the collagen triple helix.

    abstract：:The crystal structure of fiddler crab collagenase complexed with the dimeric serine protease inhibitor ecotin at 2.5 A resolution reveals an extended cleft providing binding sites for at least 11 contiguous substrate residues. Comparison of the positions of nine intermolecular main chain hydrogen bonding interactions ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi9617522

    authors： Perona JJ,Tsu CA,Craik CS,Fletterick RJ

    更新日期：1997-05-06 00:00:00

  • Ligand-free open-closed transitions of periplasmic binding proteins: the case of glutamine-binding protein.

    abstract：:The ability to undergo large-scale domain rearrangements is essential for the substrate-binding function of periplasmic binding proteins (PBPs), which are indispensable for nutrient uptake in Gram-negative bacteria. Crystal structures indicate that PBPs typically adopt either an "open" unliganded configuration or a "c...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi902045p

    authors： Bermejo GA,Strub MP,Ho C,Tjandra N

    更新日期：2010-03-09 00:00:00

  • Reduction precedes cytidylyl transfer without substrate channeling in distinct active sites of the bifunctional CDP-ribitol synthase from Haemophilus influenzae.

    abstract：:CDP-ribitol synthase is a bifunctional reductase and cytidylyltransferase that catalyzes the transformation of D-ribulose 5-phosphate, NADPH, and CTP to CDP-ribitol, a repeating unit present in the virulence-associated polysaccharide capsules of Haemophilus influenzae types a and b [Follens, A., et al. (1999) J. Bacte...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi002745n

    authors： Zolli M,Kobric DJ,Brown ED

    更新日期：2001-04-24 00:00:00

  • Mechanism of the interaction of beta(2)-glycoprotein I with negatively charged phospholipid membranes.

    abstract：:In an attempt to understand the multifunctional involvement of beta(2)-glycoprotein I (beta(2)GPI) in autoimmune diseases, thrombosis, atherosclerosis, and inflammatory processes, substantial interest is focused on the interaction of beta(2)GPI with negatively charged ligands, in particular, with acidic phospholipids....

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi0114372

    authors： Hammel M,Schwarzenbacher R,Gries A,Kostner GM,Laggner P,Prassl R

    更新日期：2001-11-27 00:00:00

  • Effect of H+ on the K+ activation of adenosine-5'-monophosphate aminohydrolase.

    abstract：:The activation of adenosine-5'-monophosphate aminohydrolase from rabbit skeletal muscle by H+ has been demonstrated. Evidence is presented which indicates that the binding of H+ and K+ is linked, in that the dissociation constant (KA) for K+ activation is reduced as the pH is lowered. Concomitantly, the pK of several ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00641a013

    authors： Campbell JC,Suelter CH

    更新日期：1977-11-01 00:00:00

  • Critical micelle concentrations of gangliosides.

    abstract：:The micellar properties of mixed, bovine gangliosides and purified galactosyl-N-acetylacetylgalactosaminyl (N-acetylneuraminyl) galactosylglucosylceramide were studied by gel filtration, equilibrium dialysis, and band and boundary centrifugation in sucrose gradients. The dissociation of micelles is very slow (days) in...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00573a028

    authors： Formisano S,Johnson ML,Lee G,Aloj SM,Edelhoch H

    更新日期：1979-03-20 00:00:00

  • Two types of hydrocarbon chain interdigitation in sphingomyelin bilayers.

    abstract：:Vibrational Raman spectroscopic experiments have been performed as a function of temperature on aqueous dispersions of synthetic DL-erythro-N-lignoceroylsphingosylphosphocholine [C(24):SPM], a racemic mixture of two highly asymmetric hydrocarbon chain length sphingomyelins. Raman spectral peak-height intensity ratios ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00343a036

    authors： Levin IW,Thompson TE,Barenholz Y,Huang C

    更新日期：1985-10-22 00:00:00