Characterization of a female-specific hepatic mitochondrial cytochrome P-450 whose steady-state level is modulated by testosterone.

abstract：:Analysis of [gamma-32P]ATP in vitro labeled nuclear RNA shows transfer of gamma-32P from ATP to form the 5'-terminal monophosphate for large RNA molecules. This finding is an actively transcribing nuclear system capable of guanylation and methylation reactions indicates that polynucleotide kinase activity in the eukar...

journal_title：Biochemistry

authors： Winicov I

更新日期：1977-09-20 00:00:00

abstract：:Structures of the DNA sequences d(CCGGCGCCGG) and d(CCAGTACbr(5)UGG) are presented here as four-way Holliday junctions in their compact stacked-X forms, with antiparallel alignment of the DNA strands. Thus, the ACC-trinucleotide motif, previously identified as important for stabilizing the junction, is now extended to...

journal_title：Biochemistry

authors： Hays FA,Vargason JM,Ho PS

更新日期：2003-08-19 00:00:00

abstract：:The interaction of the C-terminal octapeptide of cholecystokinin, CCK-8, with the third extracellular loop of human cholecystokinin-A receptor, CCK(A)-R(329-357), has been probed by high-resolution NMR and extensive computer simulations. The structure of CCK(A)-R(329-357) in the presence of dodecylphosphocholine micel...

journal_title：Biochemistry

authors： Giragossian C,Mierke DF

更新日期：2001-04-03 00:00:00

abstract：:Growth factor receptor-bound protein 14 (Grb14) is involved in growth factor receptor tyrosine kinase signaling. Here we report that light causes a major redistribution of Grb14 among the individual subcellular compartments of the retinal rod photoreceptor. Grb14 is localized predominantly to the inner segment, nuclea...

journal_title：Biochemistry

authors： Rajala A,Daly RJ,Tanito M,Allen DT,Holt LJ,Lobanova ES,Arshavsky VY,Rajala RV

更新日期：2009-06-23 00:00:00

abstract：:Replication protein A (RPA) is multisubunit single-stranded DNA-binding protein required for multiple processes in DNA metabolism including DNA replication, DNA repair, and recombination. Human RPA is a stable complex of three subunits of 70, 32, and 14 kDa (RPA70, RPA32, and RPA14, respectively). We examined the stru...

journal_title：Biochemistry

authors： Gomes XV,Henricksen LA,Wold MS

更新日期：1996-04-30 00:00:00

abstract：:G-protein-coupled receptors (GPCRs) have evolved as highly specialized cellular machinery that can dictate biological outcomes in response to diverse stimuli. Specifically, they induce multiple pathway responses upon structural perturbations induced at local protein sites. GPCRs utilize a concurrent strategy involving...

journal_title：Biochemistry

authors： Jatana N,Aswin SK,Rathore S,Thukral L

更新日期：2020-01-28 00:00:00

abstract：:Cell-signaling peptides have been extensively used to transport functional molecules across the plasma membrane into living cells. These peptides consist of a hydrophobic sequence and a cationic nuclear localization sequence (NLS). It has been assumed that the hydrophobic region penetrates the hydrophobic lipid bilaye...

journal_title：Biochemistry

authors： Ramamoorthy A,Kandasamy SK,Lee DK,Kidambi S,Larson RG

更新日期：2007-01-30 00:00:00

abstract：:Reaction of 1-oxiranylpyrene (1-OP) with DNA and the structures of the covalent and noncovalent complexes formed were studied in aqueous media (5 mM phosphate buffer with 0.1 M NaCl, pH 7) by utilizing the techniques of absorption, fluorescence and linear dichroism spectroscopy in order to gain an understanding of pos...

journal_title：Biochemistry

authors： Kim MH,Geacintov NE,Pope M,Harvey RG

更新日期：1984-11-06 00:00:00

abstract：:Glucocorticoid (GC) hormones induce apoptosis in T-cell and pre-B-cell acute lymphoblastic leukemia (ALL) cells. Steroid-mediated apoptosis requires a threshold level of the glucocorticoid receptor (GR) protein, and increasing the intracellular GR levels in ALL cells would augment their hormone sensitivity. A protein ...

journal_title：Biochemistry

authors： Geng CD,Vedeckis WV

更新日期：2010-10-19 00:00:00

abstract：:3-Deoxy-d-manno-octulosonate 8-phosphate (KDO8P) synthase catalyzes the condensation of arabinose 5-phosphate (A5P) and phosphoenolpyruvate (PEP) to form KDO8P, a key precursor in the biosynthesis of the endotoxin of Gram-negative bacteria. Earlier studies have established that the condensation occurs with a syn addit...

journal_title：Biochemistry

authors： Tao P,Gatti DL,Schlegel HB

更新日期：2009-12-15 00:00:00

abstract：:Integral membrane proteins, which include many cellular effector proteins and drug targets, can be difficult to produce, purify, and manipulate. Although the isolated ectodomains of many membrane proteins can be expressed as water soluble proteins, biological activity is frequently lost when these proteins are release...

journal_title：Biochemistry

authors： Waters EK,Morrissey JH

更新日期：2006-03-21 00:00:00

abstract：:Minor leucine tRNA species, tRNA(Leu)4 and tRNA(Leu)5, from Escherichia coli B have been reported to recognize leucine codons UUA and UUG [Goldman, E., Holmes, W. M., and Hatfield, G. W. (1979) J. Mol. Biol. 129, 567-585]. In the present study, these two tRNA(Leu) species were purified from E. coli A19, and the nucleo...

journal_title：Biochemistry

authors： Horie N,Yamaizumi Z,Kuchino Y,Takai K,Goldman E,Miyazawa T,Nishimura S,Yokoyama S

更新日期：1999-01-05 00:00:00

abstract：:C4'-oxidized (C4-AP) and C5'-oxidized abasic sites (DOB) that are produced following abstraction of a hydrogen atom from the DNA backbone reversibly form cross-links selectively with dA opposite a 3'-adjacent nucleotide, despite the comparable proximity of an opposing dA. A previous report on UvrABC incision of DNA su...

journal_title：Biochemistry

authors： Ghosh S,Greenberg MM

更新日期：2015-10-13 00:00:00

abstract：:Multisite mutagenesis of single-chain and monomeric forms of human interleukin 5 (IL-5) was performed to investigate mechanistic features of receptor activation and the possibility of differentiating sites of activation from those for receptor interaction. The normally dimeric human IL-5 contains two domains, each con...

journal_title：Biochemistry

authors： Plugariu CG,Wu SJ,Zhang W,Chaiken I

更新日期：2000-12-05 00:00:00

abstract：:The fluorescence quenching of 9-aminoacridine (9AA) in suspension of beef heart EDTA submitochondrial particles was studied and was used to calculate the pH gradient between these particles and the medium. This pH gradient, which is energy dependent, is also dependent strongly on the presence of anion species in the m...

journal_title：Biochemistry

authors： Rottenberg H,Lee CP

更新日期：1975-06-17 00:00:00

abstract：:The tertiary structure of apolipoprotein (apo) A-I and the contributions of structural domains to the properties of the protein molecule are not well defined. We used a series of engineered human and mouse apoA-I molecules in a range of physical-biochemical measurements to address this issue. Circular dichroism measur...

journal_title：Biochemistry

authors： Tanaka M,Koyama M,Dhanasekaran P,Nguyen D,Nickel M,Lund-Katz S,Saito H,Phillips MC

更新日期：2008-02-19 00:00:00

abstract：:The complex kinetic behavior commonly observed in protein folding studies suggests that a heterogeneous population of molecules exists in solution and that a number of discrete steps are involved in the conversion of unfolded molecules to the fully native form. A central issue in protein folding is whether any of thes...

journal_title：Biochemistry

authors： Walkenhorst WF,Green SM,Roder H

更新日期：1997-05-13 00:00:00

abstract：:Exogenous mannosylphosphoryldolichol (Man-P-Dol) has previously been shown to stimulate UDP-GlcNAc:dolichyl phosphate N-acetylglucosamine 1-phosphate transferase (GPT1), the enzyme catalyzing the biosynthesis of N-acetylglucosaminylpyrophosphoryldolichol (GlcNAc-P-P-Dol). To define the structural specificity of the ma...

journal_title：Biochemistry

authors： Kean EL,Rush JS,Waechter CJ

更新日期：1994-08-30 00:00:00

abstract：:Engineering proteins to withstand a broad range of conditions continues to be a coveted objective, holding the potential to advance biomedicine, industry, and our understanding of disease. One way of achieving this goal lies in elucidating the underlying interactions that define protein stability. It has been shown th...

journal_title：Biochemistry

authors： Strickler SS,Gribenko AV,Gribenko AV,Keiffer TR,Tomlinson J,Reihle T,Loladze VV,Makhatadze GI

更新日期：2006-03-07 00:00:00

abstract：:All CG dinucleotides along exons 5-8 of the p53 tumor suppressor gene contain endogenous 5-methylcytosine (MeC). These same sites (e.g., codons 157, 158, 245, 248, and 273) are mutational hot spots in smoking-induced lung cancer. Several groups used the UvrABC endonuclease incision assay to demonstrate that methylated...

journal_title：Biochemistry

authors： Ziegel R,Shallop A,Upadhyaya P,Jones R,Tretyakova N

更新日期：2004-01-20 00:00:00

abstract：:To characterize the roles played by Glu44 and Glu56 of cytochrome b5 in the formation of the electrostatic complex between cytochrome c and cytochrome b5, the Glu44, Glu56, or both sites were changed to alanine by site-directed mutagenesis. The influence of these two residues on the protein stability was probed by inv...

journal_title：Biochemistry

authors： Qian W,Sun YL,Wang YH,Zhuang JH,Xie Y,Huang ZX

更新日期：1998-10-06 00:00:00

abstract：:The amidotransferase family of enzymes utilizes the ammonia derived from the hydrolysis of glutamine for a subsequent chemical reaction catalyzed by the same enzyme. The ammonia intermediate does not dissociate into solution during the chemical transformations. A well-characterized example of the structure and mechani...

journal_title：Biochemistry

authors： Raushel FM,Thoden JB,Holden HM

更新日期：1999-06-22 00:00:00

abstract：:Prothrombin is the precursor of thrombin, a central enzyme in coagulation. Autoantibodies to prothrombin are associated with thromboembolism, but the mechanisms by which the antibodies modulate the coagulation processes are not understood. We screened a panel of 34 monoclonal antibody light chains isolated from patien...

journal_title：Biochemistry

authors： Thiagarajan P,Dannenbring R,Matsuura K,Tramontano A,Gololobov G,Paul S

更新日期：2000-05-30 00:00:00

abstract：:At any instant, the human erythrocyte sugar transporter presents at least one sugar export site but multiple sugar import sites. The present study asks whether the transporter also presents more than one sugar exit site. We approached this question by analysis of binding of [3H]cytochalasin B (an export conformer liga...

journal_title：Biochemistry

authors： Cloherty EK,Levine KB,Carruthers A

更新日期：2001-12-25 00:00:00

abstract：:The human ferritin L-chain cDNA was cloned into a vector for overproduction in Escherichia coli, under the regulation of a lambda promoter. The plasmid obtained contains the full L-chain coding region modified at the first two codons. It is able to direct the synthesis of the L-chain which can constitute up to 15% of ...

journal_title：Biochemistry

authors： Levi S,Salfeld J,Franceschinelli F,Cozzi A,Dorner MH,Arosio P

更新日期：1989-06-13 00:00:00

abstract：:Ketosteroid isomerase (KSI) is one of the most proficient enzymes catalyzing an allylic isomerization reaction at a diffusion-controlled rate. In this study of KSI, we have detailed the structures of its active site, the role of various catalytic residues, and have explained the origin of the its fast reactivity by ca...

journal_title：Biochemistry

authors： Oh KS,Cha SS,Kim DH,Cho HS,Ha NC,Choi G,Lee JY,Tarakeshwar P,Son HS,Choi KY,Oh BH,Kim KS

更新日期：2000-11-14 00:00:00

abstract：:Expansions of (CTG)·(CAG) repeated DNAs are the mutagenic cause of 14 neurological diseases, likely arising through the formation and processing of slipped-strand DNAs. These transient intermediates of repeat length mutations are formed by out-of-register mispairing of repeat units on complementary strands. The three-...

journal_title：Biochemistry

authors： Slean MM,Reddy K,Wu B,Nichol Edamura K,Kekis M,Nelissen FH,Aspers RL,Tessari M,Schärer OD,Wijmenga SS,Pearson CE

更新日期：2013-02-05 00:00:00

abstract：:The zinc-dependent metallo-beta-lactamases are a group of bacterial enzymes that pose a threat to the future efficacy of present-day antibiotics. Their mechanism is poorly understood, and there are no clinically useful inhibitors. While most members of the group contain two tightly bound zinc ions in their active site...

journal_title：Biochemistry

authors： Davies AM,Rasia RM,Vila AJ,Sutton BJ,Fabiane SM

更新日期：2005-03-29 00:00:00

abstract：:Specific binding of glutamate to its carrier was investigated by using cytoplasmic membrane vesicles prepared from Escherichia coli B. The binding activity was specifically affected by the Na+ and H+ concentrations of the medium. Cytoplasmic membrane vesicles from the mutant strain 36-39 that is defective in the Na+-d...

journal_title：Biochemistry

authors： Fujimura T,Yamato I,Anraku Y

更新日期：1983-04-12 00:00:00

abstract：:Activator of G protein signaling 3 (AGS3) activates the Gbetagamma mating pathway in yeast in a manner that is independent of heptahelical receptors. It competes with Gbetagamma subunits to bind GDP-bound Gi/o(alpha) subunits via four repeated G protein regulatory (GPR) domains in the carboxyl-terminal half of the mol...

journal_title：Biochemistry

authors： Ma H,Peterson YK,Bernard ML,Lanier SM,Graber SG

更新日期：2003-07-08 00:00:00