Properties and regulation of the catalytic domain of Ira2p, a Saccharomyces cerevisiae GTPase-activating protein of Ras2p.

abstract：:The noncovalent interaction of 2-aminodipyrido[1,2-a:3',2'-d]imidazole (Glu-P-2) and its derivatives, which are potent mutagens isolated from L-glutamic acid pyrolysate, with calf thymus DNA was studied by steady-state and nanosecond fluorescence spectroscopies. The fluorescence of these compounds exhibits static quen...

journal_title：Biochemistry

authors： Itoh M,Kurokawa H,Usui M,Ohno M,Shimoda E,Hashimoto Y,Shudo K

更新日期：1985-11-05 00:00:00

abstract：:The three-dimensional solution structure of toxin FS2, a 60-residue polypeptide isolated from the venom of black mamba snake (Dendroaspis polylepis polylepis), has been determined by nuclear magnetic resonance spectroscopy. Using 600 NOE constraints and 55 dihedral angle constraints, a set of 20 structures obtained fr...

journal_title：Biochemistry

authors： Albrand JP,Blackledge MJ,Pascaud F,Hollecker M,Marion D

更新日期：1995-05-02 00:00:00

abstract：:Surface plasmon resonance (SPR) detection technology was employed to investigate the kinetic mechanism of deoxyadenosine kinase from Mycoplasma mycoides ssp. mycoides SC. In our experimental approach, the enzyme was attached to the sensor surface, the reactants were injected in the mobile phase, and the product-enzyme...

journal_title：Biochemistry

authors： Pol E,Wang L

更新日期：2006-01-17 00:00:00

abstract：:Our previous studies revealed that interaction of fibrin with the very low density lipoprotein (VLDL) receptor plays a prominent role in transendothelial migration of leukocytes and thereby inflammation. The major goal of our subsequent studies is to establish the structural basis for this interaction. As the first st...

journal_title：Biochemistry

authors： Banerjee K,Yakovlev S,Gruschus JM,Medved L,Tjandra N

更新日期：2018-07-31 00:00:00

abstract：:X-ray studies of the proofreading 3',5'-exonuclease site of the large (Klenow) fragment of DNA polymerase I have detected a binuclear metal complex consisting of a pentacoordinate metal (site A) which shares a ligand, Asp-355, with an octahedral metal (site B) [Freemont, P. S., Friedman, J. M., Beese, L. S., Sanderson...

journal_title：Biochemistry

authors： Han H,Rifkind JM,Mildvan AS

更新日期：1991-11-19 00:00:00

abstract：:The nature of the putative general acid His187 in the reaction catalyzed by Escherichia coli uracil DNA glycosylase (UDG) was investigated using X-ray crystallography and NMR spectroscopy. The crystal structures of H187Q UDG, and its complex with uracil, have been solved at 1.40 and 1.60 A resolution, respectively. Th...

journal_title：Biochemistry

authors： Drohat AC,Xiao G,Tordova M,Jagadeesh J,Pankiewicz KW,Watanabe KA,Gilliland GL,Stivers JT

更新日期：1999-09-14 00:00:00

abstract：:Mouse photoreceptor function and survival critically depend on Ca(2+)-regulated retinal membrane guanylyl cyclase (RetGC), comprised of two isozymes, RetGC1 and RetGC2. We characterized the content, catalytic constants, and regulation of native RetGC1 and RetGC2 isozymes using mice lacking guanylyl cyclase activating ...

journal_title：Biochemistry

authors： Peshenko IV,Olshevskaya EV,Savchenko AB,Karan S,Palczewski K,Baehr W,Dizhoor AM

更新日期：2011-06-28 00:00:00

abstract：:Although the mechanism by which the steroidogenic acute regulatory protein (StAR) promotes steroidogenesis has been studied extensively, it remains incompletely characterized. Because structural analysis has revealed a hydrophobic sterol-binding pocket (SBP) within StAR, this study sought to examine the regulatory rol...

journal_title：Biochemistry

authors： Rajapaksha M,Kaur J,Bose M,Whittal RM,Bose HS

更新日期：2013-10-15 00:00:00

abstract：:The crystal structures of 7,8-dimethylisoalloxazine-10-acetic acid-tryptamine (1:1) tetrahydrate and 7,8-dimethylisoalloxazine-10-acetic acid-tyramine (1:1) tetrahydrate complexes were determined by the X-ray diffraction method, as models for flavin-tryptophan and flavin-tyrosine interactions in flavoproteins. The obs...

journal_title：Biochemistry

authors： Inoue M,Shibata M,Kondo Y,Ishida T

更新日期：1981-05-12 00:00:00

abstract：:HIV-1 RNA dimerization is a complex process that involves a series of RNA refolding events. The monomeric RNA can adopt two alternative conformations that largely determine the efficiency of dimerization. The dimeric RNA also exists in two different conformations, an initial kissing-loop complex and a stable dimer wit...

journal_title：Biochemistry

authors： Huthoff H,Berkhout B

更新日期：2002-08-20 00:00:00

abstract：:Scaffold/matrix-attached regions (S/MARs) are cis-acting elements with a function outside transcribed regions and in introns. Although they usually augment transcriptional rates, their action is highly context-dependent. We cloned an 800 bp S/MAR element from the upstream border of the human interferon-beta domain at ...

journal_title：Biochemistry

authors： Schübeler D,Mielke C,Maass K,Bode J

更新日期：1996-08-27 00:00:00

abstract：:The binding of agonists, antagonists, and the acetylcholinesterase inhibitor, eserine, to the nicotinic acetylcholine receptor from Torpedo californica has been monitored by the fluorescence changes of two extrinsic probes that have been covalently attached to the receptor protein. Although both probes, 5-(iodoacetami...

journal_title：Biochemistry

authors： Dunn SM,Raftery MA

更新日期：1993-08-24 00:00:00

abstract：:The bacterium Caulobacter crescentus is known to attach irreversibly to underwater surfaces by utilizing an adhesive structure called the holdfast, which exhibits the greatest known adhesive strength of any organism. The very small size of the holdfast (∼400 nm wide and ∼40 nm high) has made direct chemical analysis d...

journal_title：Biochemistry

authors： Nyarko A,Singla S,Barton HA,Dhinojwala A

更新日期：2020-09-22 00:00:00

abstract：:Two heat-shock proteins that show high identity with the Escherichia coli chaperonin 60 (groEL) and chaperonin 10 (groES) chaperonin proteins were purified and characterized from photolithoautotrophically grown Rhodobacter sphaeroides. The proteins were purified by using sucrose density gradient centrifugation and Mon...

journal_title：Biochemistry

authors： Terlesky KC,Tabita FR

更新日期：1991-08-20 00:00:00

abstract：:The kinetics of Zn(2+) binding by two point-mutated forms of the HIV-1 NCp7 C-terminal zinc finger, each containing tridentate binding motif HCC [Ser49(35-50)NCp7] or CCC [Ala44(35-50)NCp7], has been studied by stopped-flow spectrofluorimetry. Both the formation and dissociation rate constants of the complexes between...

journal_title：Biochemistry

authors： Bombarda E,Roques BP,Mély Y,Grell E

更新日期：2005-05-17 00:00:00

abstract：:Perioperative bleeding is a cause of major blood loss and is associated with increased rates of postoperative morbidity and mortality. To combat this, antifibrinolytic inhibitors of the serine protease plasmin are commonly used to reduce bleeding during surgery. The most effective and previously widely used of these i...

journal_title：Biochemistry

authors： Swedberg JE,Harris JM

更新日期：2011-10-04 00:00:00

abstract：:The skeletal muscle Ca2+ release channel (RYR1) is regulated by calmodulin in both its Ca2+-free (apocalmodulin) and Ca2+-bound (Ca2+ calmodulin) states. Apocalmodulin is an activator of the channel, and Ca2+ calmodulin is an inhibitor of the channel. Both apocalmodulin and Ca2+ calmodulin binding sites on RYR1 are de...

journal_title：Biochemistry

authors： Moore CP,Rodney G,Zhang JZ,Santacruz-Toloza L,Strasburg G,Hamilton SL

更新日期：1999-06-29 00:00:00

abstract：:alpha 1-Adrenergic receptor probes, which can be radioiodinated to yield high specific activity radioligands, have been synthesized and characterized. 2-[4-(4-Amino-benzoyl)piperazin-1-yl]-4-amino-6,7-dimethoxyquin azoline (CP63,155), an arylamine analogue of the selective alpha 1-adrenergic antagonist prazosin, and i...

journal_title：Biochemistry

authors： Seidman CE,Hess HJ,Homcy CJ,Graham RM

更新日期：1984-07-31 00:00:00

abstract：:Sumoylation, the process by which the ubiquitin-related SUMO protein is covalently attached to lysine side chains in other proteins, is involved in numerous processes in the eukaryotic cell, including transcriptional repression. In this study, we identify Gcn5, the histone-modifying subunit of the transcriptional regu...

journal_title：Biochemistry

authors： Sterner DE,Nathan D,Reindle A,Johnson ES,Berger SL

更新日期：2006-01-24 00:00:00

abstract：:Single-tryptophan-containing mutants of low adenylation state Escherichia coli glutamine synthetase (wild type has two tryptophans at positions 57 and 158) have been constructed and studied by multifrequency phase/modulation fluorescence spectroscopy. The W57L mutant (retains tryptophan at residue 158) and the W158S m...

journal_title：Biochemistry

authors： Atkins WM,Stayton PS,Villafranca JJ

更新日期：1991-04-09 00:00:00

abstract：:A full characterization of the thermodynamic forces underlying ligand-associated conformational changes in proteins is essential for understanding and manipulating diverse biological processes, including transport, signaling, and enzymatic activity. Recent experiments on the maltose binding protein (MBP) have provided...

journal_title：Biochemistry

authors： Bucher D,Grant BJ,McCammon JA

更新日期：2011-12-06 00:00:00

abstract：:The reaction of cytochrome c oxidase (COX) from Rhodobacter sphaeroides with hydrogen peroxide has been studied at alkaline (pH 8.5) and acidic (pH 6.5) conditions with the aid of a stopped-flow apparatus. Absorption changes in the entire 350-800 nm spectral range were monitored and analyzed by a global fitting proced...

journal_title：Biochemistry

authors： Pecoraro C,Gennis RB,Vygodina TV,Konstantinov AA

更新日期：2001-08-14 00:00:00

abstract：:Measurement of x-ray scattering at very small angles by solutions of myosin subfragment 1 (S1) yields a radius of gyration of 3.24 nm (mean) +/- 0.03 nm (standard error for N = 9). If S1 is assumed to be ellipsoid of revolution, of uniform electron density, with a molecular weight of 1.15 X 10(5) and a partial specifi...

journal_title：Biochemistry

authors： Kretzschmar KM,Mendelson RA,Morales MF

更新日期：1978-06-13 00:00:00

abstract：:Kinetic models are presented that allow the Na(+),K(+)-ATPase steady-state turnover number to be estimated at given intra- and extracellular concentrations of Na(+), K(+), and ATP. Based on experimental transient kinetic data, the models utilize either three or four steps of the Albers-Post scheme, that is, E(2) --> E...

journal_title：Biochemistry

authors： Kong BY,Clarke RJ

更新日期：2004-03-02 00:00:00

abstract：:We examined the interactions of nucleotides with the CMP-sialic acid transporter in order to better understand which features play a role in binding and to investigate the relationship between binding and subsequent transport. With respect to the sugar, the transporter requires a complete ribose ring for tight binding...

journal_title：Biochemistry

authors： Chiaramonte M,Koviach JL,Moore C,Iyer VV,Wagner CR,Halcomb RL,Miller W,Melançon P,Kuchta RD

更新日期：2001-11-27 00:00:00

abstract：:Protein serine/threonine phosphatase 2A (PP2A) is a critical regulator of numerous cellular signaling processes and a potential target for reactive electrophiles that dysregulate phosphorylation-dependent signal transduction cascades. The predominant cellular form of PP2A is a heterotrimeric holoenzyme consisting of a...

journal_title：Biochemistry

authors： Codreanu SG,Adams DG,Dawson ES,Wadzinski BE,Liebler DC

更新日期：2006-08-22 00:00:00

abstract：:We studied the binding characteristics of a synthetic 20-residue peptide to supported single planar bilayers of phosphatidylcholine, and the orientation of the peptide by Fourier-transform infrared spectroscopy with an attenuated total reflection method. This peptide, designed to resemble a putative fusion peptide of ...

journal_title：Biochemistry

authors： Ishiguro R,Matsumoto M,Takahashi S

更新日期：1996-04-16 00:00:00

abstract：:Artificial genetic systems have been developed by synthetic biologists over the past two decades to include additional nucleotides that form additional nucleobase pairs independent of the standard T:A and C:G pairs. Their use in various tools to detect and analyze DNA and RNA requires polymerases that synthesize duple...

journal_title：Biochemistry

authors： Laos R,Shaw R,Leal NA,Gaucher E,Benner S

更新日期：2013-08-06 00:00:00

abstract：:It is generally accepted that electron transfer in bacterial photosynthesis is driven by the first singlet excited state of a special pair of bacteriochlorophylls (P*). We have examined the first steps of electron transfer in a mutant of the Rhodobacter sphaeroides reaction center in which charge separation from P* is...

journal_title：Biochemistry

authors： Van Brederode ME,Jones MR,Van Mourik F,Van Stokkum IH,Van Grondelle R

更新日期：1997-06-10 00:00:00

abstract：:In aspartate aminotransferase, pyridoxal 5'-phosphate (PLP) forms a Schiff base with the epsilon-amino group of Lys258 (internal aldimine). The internal aldimine has a pKa value of 6.8. Binding of a substrate amino acid to the enzyme yields the Michaelis complex, in which PLP still forms the internal aldimine with Lys...

journal_title：Biochemistry

authors： Hayashi H,Mizuguchi H,Kagamiyama H

更新日期：1998-10-27 00:00:00