Abstract：
:The bacterium Caulobacter crescentus is known to attach irreversibly to underwater surfaces by utilizing an adhesive structure called the holdfast, which exhibits the greatest known adhesive strength of any organism. The very small size of the holdfast (∼400 nm wide and ∼40 nm high) has made direct chemical analysis difficult, and its structure remains poorly understood. In this study, we employ spectroscopic techniques, including attenuated total reflection infrared spectroscopy (ATR-IR) and X-ray photoelectron spectroscopy, to probe holdfast chemistry. The data indicate the presence of a peptide signal within the holdfast polymer. By comparing the ATR-IR spectrum of the holdfast to peptidoglycan spectra from other bacterial species, we demonstrate the similarity of the holdfast chemistry to that of peptidoglycan, suggesting peptide cross-linking may play a role in holdfast architecture. To probe the molecular groups at the interface, surface-sensitive sum frequency generation spectroscopy was used to show that aromatic and hydroxyl groups related to this protein content at the adhesive interface could be playing a crucial role in adhesion. On the basis of these results, we propose a model of the holdfast architecture with similarities to the peptide cross-linking observed in the peptidoglycan polymer of the bacterial cell wall. These results not only provide information about the development of adhesives that could be based on holdfast chemical architecture but also reveal a potentially yet unexplored biosynthetic pathway in holdfast synthesis that has not yet been revealed by genetic approaches, thereby opening up a potentially new avenue of research in holdfast synthesis.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Nyarko A,Singla S,Barton HA,Dhinojwala Adoi
10.1021/acs.biochem.0c00625subject
Has Abstractpub_date
2020-09-22 00:00:00pages
3508-3516issue
37eissn
0006-2960issn
1520-4995journal_volume
59pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract：:The NuoD segment (homologue of mitochondrial 49 kDa subunit) of the proton-translocating NADH:quinone oxidoreductase (complex I/NDH-1) from Escherichia coli is in the hydrophilic domain and bears many highly conserved amino acid residues. The three-dimensional structural model of NDH-1 suggests that the NuoD segment, ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi501403t
更新日期：2015-01-27 00:00:00
abstract：:Molecular chaperones of the heat shock protein 70 (Hsp70) family counteract protein misfolding in a variety of neurodegenerative disease models. To determine whether human Hsp70 exerts similar effects on the aggregation of alpha-synuclein (alpha-Syn), the key component of insoluble fibrils present in Parkinson's disea...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi801475r
更新日期：2008-11-25 00:00:00
abstract：:Chicken nucleoside triphosphate diphosphohydrolase 8 (NTPDase8) is a cell surface ectonucleotidase with a large extracellular domain (ECD) containing the active site and is anchored to the membrane by two transmembrane domains (TMDs) at the N- and C-termini. Unlike other cell surface NTPDases that have been characteri...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi901820c
更新日期：2010-01-12 00:00:00
abstract：:The interaction of the TAR RNA element of human immunodeficiency virus type 1 (HIV-1) with a 2'-O-methyl analogue of an RNA hairpin aptamer previously identified by in vitro selection [Ducongé, F., and Toulmé, J. J. (1999) RNA 5, 1605-1614] was characterized by UV-monitored thermal denaturation and surface plasmon res...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi025974d
更新日期：2002-10-08 00:00:00
abstract：:Isolated chloroplasts are capable of synthesizing chloroplast DNA in the presence of Mg2+ and deoxynucleoside triphosphates. The in vitro reaction proceeds for at least 60 min and is inhibited by KC1 and N-ethylmaleimide. Stretches of several hundred nucleotides in length are synthesized within an hour. Little or no i...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00257a014
更新日期：1982-07-06 00:00:00
abstract：:The structural protein nonerythroid alpha spectrin (alphaIISp) plays a role in the repair of DNA interstrand cross-links and is deficient in cells from patients with Fanconi anemia (FA), in which there is a defect in ability to repair such cross-links. We have proposed a model in which alphaIISp, whose stability is de...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi801483u
更新日期：2009-01-20 00:00:00
abstract：:We have measured the rates and efficiencies of DNA unwinding (the number of ATP molecules hydrolyzed per DNA base pair unwound) catalyzed by the RecBC,RecBCD-K177Q (a site-directed mutant in the putative ATP-binding site in the RecD subunit), and RecBCD enzymes from Escherichia coli. The DNA unwinding rate was measure...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00198a022
更新日期：1994-08-16 00:00:00
abstract：:HIV-1 Vif is an accessory protein that induces the proteasomal degradation of the host restriction factor, apolipoprotein B mRNA-editing enzyme catalytic polypeptide-like 3G (APOBEC3G). The N-terminal half of Vif binds to APOBEC3G, and the C-terminal half binds to subunits of a cullin 5-based ubiquitin ligase. This Vi...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi201738a
更新日期：2012-03-13 00:00:00
abstract：:The light-driven enzyme NADPH:protochlorophyllide oxidoreductase (POR) catalyzes the reduction of protochlorophyllide (Pchlide) to chlorophyllide (Chlide), a key regulatory step in the chlorophyll biosynthesis pathway. As POR is light activated, it allows intermediates in the reaction pathway to be observed by initiat...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0268448
更新日期：2003-01-21 00:00:00
abstract：:Salmonella typhimurium L-histidinol dehydrogenase (EC 1.1.1.23), a four-electron dehydrogenase, was inactivated by an active-site-directed modification reagent, 7-chloro-4-nitro-2,1,3-benzoxadiazole (NBD-Cl). The inactivation followed pseudo-first-order kinetics and was prevented by low concentrations of the substrate...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00365a009
更新日期：1986-08-26 00:00:00
abstract：:A five-residue loop structure in basic fibroblast growth factor (FGF-2) which extends from amino acid residue 118 to residue 122 was replaced, by cassette mutagenesis, with the corresponding seven-residue loop structure from the structural homologue acidic fibroblast growth factor (FGF-1) or the corresponding five-res...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00003a004
更新日期：1995-01-24 00:00:00
abstract：:The second-order electron transfer reaction between cytochrome b5 and cytochrome c has been studied by cyclic voltammetry utilizing a gold electrode modified with beta-mercaptopropionate. When cyclic voltammetry is performed on a solution containing a mixture of cytochrome b5, cytochrome c and polylysine, cytochrome b...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi9529723
更新日期：1996-09-24 00:00:00
abstract：:The rates of inactivation of BF1 adenosinetriphosphatase (BF1-ATPase) from Escherichia coli by 7-chloro-4-nitro-2,1,3-benzoxadiazole, 1-fluoro-2,4-dinitrobenzene, 2,4,6-trinitrobenzenesulfonate, phenylglyoxal, and N,N'-dicyclohexylcarbodiimide have been measured in the presence and absence of various concentrations of...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00531a011
更新日期：1982-01-19 00:00:00
abstract：:In this work, we have studied the role of the arginine finger region in determining the specificity of the GTPase activating proteins (GAPs) Saccharomyces cerevisiae Ira2p and human p120-GAP toward yeast Ras2p and human Ha-Ras p21. It is known that p120-GAP can enhance both Ras2p and Ha-Ras GTPase activities, whereas ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi010027a
更新日期：2001-06-26 00:00:00
abstract：:The complete coordination scheme for Mn(II) in transition-state-analogue complexes with creatine kinase has been determined by electron paramagnetic resonance (EPR) spectroscopy. Perturbations in the EPR spectra for Mn(II) due to superhyperfine coupling to 17O of selectively labeled ligands have been used to identify ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00565a002
更新日期：1980-11-25 00:00:00
abstract：:The SCF ubiquitin ligase associates with substrates through its F-box protein adaptor. Substrates are typically recognized through a defined phosphodegron. Here, we characterize the interaction of the F-box protein Saf1 with Prb1, one of its vacuolar protease substrates. We show that Saf1 binds the mature protein but ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.5b00504
更新日期：2015-07-28 00:00:00
abstract：:Oligonucleotide-directed mutagenesis in vivo was used to create destabilizing site specific changes at position 6 in iso-1-cytochrome c in Saccharomyces cerevisiae. These changes were made in combination with the stabilizing N52I substitution. The resulting proteins showed that a variety of forces and interactions are...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00021a023
更新日期：1995-05-30 00:00:00
abstract：:Phospholipase A2 from Naja naja kaouthia venom was covalently coupled onto agarose beads using two different chemistries. The effect of micellar competitive inhibitors in the coupling media was evaluated. Enzyme bound to N-hydroxysuccinimide-activated agarose, which is reactive primarily toward epsilon-amino groups, h...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00083a007
更新日期：1993-08-17 00:00:00
abstract：:To probe attitudinal features of the Ca(2+)-deficient site (site I) in the Ca2+ switch of cardiac troponin C (cTnC), we have examined steady-state fluorescence emission and polarization of a Trp26 inserted in a recombinant cardiac TnC (cTnC3.W) and compared these with the properties of the Ca(2+)-competent site I in s...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00002a022
更新日期：1995-01-17 00:00:00
abstract：:FHA domains are phosphoThr recognition modules found in diverse signaling proteins, including kinase-associated protein phosphatase (KAPP) from Arabidopsis thaliana. The kinase-interacting FHA domain (KI-FHA) of KAPP targets it to function as a negative regulator of some receptor-like kinase (RLK) signaling pathways i...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi061763n
更新日期：2007-03-13 00:00:00
abstract：:The Z and E isomers of 6-(methoxymethylene)-penicillanic acid have been synthesized, and their interaction with the RTEM beta-lactamase has been studied. The Z isomer is an inhibitor and an inactivator of the enzyme, and there is some similarity between its behavior and that of other mechanism-based inactivators such ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00319a025
更新日期：1984-11-20 00:00:00
abstract：:hTom20 is an outer mitochondrial membrane receptor involved in protein translocation. The cytosolic domain (aa30-145) and selected truncated versions of this domain were overexpressed and purified to study the structure-function relationship of this protein. Our studies reveal that the secondary structure of the cytos...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi980746y
更新日期：1998-09-22 00:00:00
abstract：:The non-typeable Haemophilus influenzae strain DH1 was isolated from a 25 year old male patient with Fisher syndrome, a postinfectious autoimmune condition characterized by the presence of anti-GQ1b IgG antibodies that target and initiate damage to peripheral nerves. DH1 was found to display an alphaNeuAc(2-8)alphaNeu...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi700685s
更新日期：2007-07-10 00:00:00
abstract：:Dihydroorotates deuteriated at both C5 and C6 have been prepared and used to probe the mechanism of the bovine liver mitochondrial dihydroorotate dehydrogenase. Primary deuterium isotope effects on kcat are observed with both (6RS)-[5(S)-2H]- and (6RS)-[6-2H] dihydroorotates (3 and 6, respectively); these effects are ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00429a041
更新日期：1989-02-07 00:00:00
abstract：:The BARD1 N-terminal RING domain binds BRCA1 while the BARD1 C-terminal ankyrin and tandem BRCT repeat domains bind CstF-50 to modulate mRNA processing and RNAP II stability in response to DNA damage. Here we characterize the BARD1 structural biochemistry responsible for CstF-50 binding. The crystal structure of the B...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi801115g
更新日期：2008-11-04 00:00:00
abstract：:S-Adenosylmethionine decarboxylase is a pyruvate-dependent enzyme. The enzyme forms a Schiff base with substrate, S-adenosylmethionine, through the pyruvoyl moiety. This facilitates the release of CO2 from the substrate, which must then be protonated on the alpha carbon in order to permit hydrolysis of the Schiff base...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi9825201
更新日期：1999-02-23 00:00:00
abstract：:Disruption of the zinc-thiolate center at the active site of yeast alcohol dehydrogenase results in inactivation and zinc release. Measurements of activity, zinc release, and thiol/thiolate oxidation were used to assess the effects of biologically relevant oxidants on alcohol dehydrogenase. Alcohol dehydrogenase was i...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00011a008
更新日期：1995-03-21 00:00:00
abstract：:Interleukin-8 (IL-8), a member of the chemokine superfamily, exists as both monomers and dimers, and mediates its function by binding to neutrophil CXCR1 and CXCR2 receptors that belong to the G protein-coupled receptor class. It is now well established that the monomer functions as a high-affinity ligand, but the bin...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0605944
更新日期：2006-06-27 00:00:00
abstract：:Lactose repressor protein, regulator of lac enzyme expression in Escherichia coli, maintains its structure and function at extremely low protein concentrations (<10(-)12 M). To examine the unfolding and dissociation of this tetrameric protein, structural transitions in the presence of varying concentrations of urea we...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi9900727
更新日期：1999-05-18 00:00:00
abstract：:Various omega-halogenated carboxy acids and amides were evaluated as potential active-site-directed reagents for alcohol dehydrogenase. 2-Bromoacetamide and bromoacetic and 3-bromopropionic acids inactivated the enzyme; AMP, NAD+, and NADH markedly decreased the rate of inactivation. Some omega-halogenated carboxyamid...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00694a034
更新日期：1975-11-18 00:00:00
