Multiple secondary structure rearrangements during HIV-1 RNA dimerization.

abstract：:There are highly conserved hydrogen bonds between the distal His and the adjacent Asn in many peroxidases. Although the crystal structure of horseradish peroxidase C (HRP) is not available, comparison of the amino acid sequence with cytochrome c peroxidase indicates that Asn70 is making the hydrogen bond with the dist...

journal_title：Biochemistry

authors： Nagano S,Tanaka M,Ishimori K,Watanabe Y,Morishima I

更新日期：1996-11-12 00:00:00

abstract：:Erwinia ligand-gated ion channel (ELIC) is a bacterial homologue of vertebrate pentameric ligand-gated ion channels (pLGICs) and has proven to be a valuable model for understanding the structure and function of this important protein family. There is nevertheless still a question about whether molecular details can be...

journal_title：Biochemistry

authors： Mosesso R,Dougherty DA,Lummis SCR

更新日期：2018-07-10 00:00:00

abstract：:Manganese peroxidase (MnP) from Phanerochaete chrysosporium undergoes a pH-dependent conformational change evidenced by changes in the electronic absorption spectrum. This high- to low-spin alkaline transition occurs at approximately 2 pH units lower in an F190I mutant MnP when compared to the wild-type enzyme. Herein...

journal_title：Biochemistry

authors： Youngs HL,Moënne-Loccoz P,Loehr TM,Gold MH

更新日期：2000-08-15 00:00:00

abstract：:Apolipoprotein E (apoE)-knockout mice develop severe atherosclerosis associated with high levels of very low-density lipoprotein (VLDL) and intermediate-density lipoprotein (IDL) in plasma. To investigate the atherogenic role of VLDL and IDL, the lipoprotein fraction containing both VLDL and IDL (apoEko-VLDL/IDL) was ...

journal_title：Biochemistry

authors： Hakamata H,Sakaguchi H,Zhang C,Sakashita N,Suzuki H,Miyazaki A,Takeya M,Takahashi K,Kitamura N,Horiuchi S

更新日期：1998-09-29 00:00:00

abstract：:Dynamic light scattering measurements were carried out on secretin in aqueous solution (2 mM; pH 5.0). The results indicated that the molecule exists as a fairly compact hexamer under these solution conditions. Secondary structural properties of the secretin hexameric complex were evaluated using CD and NMR spectrosco...

journal_title：Biochemistry

authors： Carpenter KA,Schiller PW

更新日期：1998-12-01 00:00:00

abstract：:Complement components C5b-6 and C7 assemble to form C5b-7, which then interacts with membranes and commits the membrane attack complex to a target site. This protein-membrane association event was investigated to determine possible structural features that could contribute to a selective membrane attack. This system m...

journal_title：Biochemistry

authors： Silversmith RE,Nelsestuen GL

更新日期：1986-11-18 00:00:00

abstract：:Membrane vesicles isolated from Escherichia coli ML 308--225 have been analyzed by crossed immunoelectrophoresis, and immunoprecipitates corresponding to the following cellular components have been identified: ATPase (EC 3.6.1,3), two or three NADH dehydrogenases (EC 1.6.99.3), D-lactate dehydrogenase (EC 1.1.1.27), g...

journal_title：Biochemistry

authors： Owen P,Kaback HR

更新日期：1979-04-17 00:00:00

abstract：:The hallmark of serpins is the ability to undergo the so-called "stressed-to-relaxed" switch during which the surface-exposed reactive center loop (RCL) becomes incorporated as strand 4 in central beta-sheet A. RCL insertion drives not only the inhibitory reaction of serpins with their target serine proteases but also...

journal_title：Biochemistry

authors： Madsen JB,Dupont DM,Andersen TB,Nielsen AF,Sang L,Brix DM,Jensen JK,Broos T,Hendrickx ML,Christensen A,Kjems J,Andreasen PA

更新日期：2010-05-18 00:00:00

abstract：:The virally encoded integrase protein carries out retroviral integration, which requires specific interactions with the two ends of the viral DNA, and also with host DNA that is the target of integration. We attached a photo-cross-linking agent to specific viral and target DNA sites to identify regions of the integras...

journal_title：Biochemistry

authors： Heuer TS,Brown PO

更新日期：1998-05-12 00:00:00

abstract：:We report two small molecule azobenzenesulfonamide probes, CAP1 and CAP2, capable of photomodulating the activity of carbonic anhydrase (CA) on demand. In the trans form, CAP azobenzene probes adopt a linear shape, making them suitable for occupying the CA active site and interacting with Zn2+, thereby inhibiting enzy...

journal_title：Biochemistry

authors： Aggarwal K,Banik M,Medellin B,Que EL

更新日期：2019-01-08 00:00:00

abstract：:Globotriaosylceramide [(Gal alpha 1-4Gal beta 1-4Glc-ceramide (Gb3)] was separated from human kidney, and the fatty acid composition was determined. Semisynthetic Gb3 molecular species of corresponding fatty acid chain length were prepared and compared for verotoxin (VT) binding affinity by TLC overlay, and a quantita...

journal_title：Biochemistry

authors： Pellizzari A,Pang H,Lingwood CA

更新日期：1992-02-11 00:00:00

abstract：:Hydrogenases are metalloenzymes that catalyze the reversible reduction of protons at unusual metal centers. This Current Topic discusses recent advances in elucidating the steps involved in the biosynthesis of the complex metal cluster at the [FeFe]-hydrogenase (HydA) active site, known as the H-cluster. The H-cluster...

journal_title：Biochemistry

authors： Shepard EM,Mus F,Betz JN,Byer AS,Duffus BR,Peters JW,Broderick JB

更新日期：2014-07-01 00:00:00

abstract：:The dehaloperoxidase-hemoglobin (DHP) from the terebellid polychaete Amphitrite ornata is a multifunctional hemoprotein that catalyzes the oxidation of a wide variety of substrates, including halo/nitrophenols, haloindoles, and pyrroles, via peroxidase and/or peroxygenase mechanisms. To probe whether substrate substit...

journal_title：Biochemistry

authors： McGuire AH,Carey LM,de Serrano V,Dali S,Ghiladi RA

更新日期：2018-07-31 00:00:00

abstract：:Porcine pancreatic phospholipase A2 interacts with micelles of the substrate analogue n-octadecylphosphocholine to form a specific complex over considerably wide concentration ranges of both lipid and protein. UV absorption difference spectroscopy measurements indicate that the ratio of lipid to protein molecules in t...

journal_title：Biochemistry

authors： Hille JD,Donné-Op den Kelder GM,Sauve P,de Haas GH,Egmond MR

更新日期：1981-07-07 00:00:00

abstract：:Life span regulation and inhibition of gene silencing in yeast have been linked to nicotinamide effects on Sir2 enzymes. The Sir2 enzymes are NAD(+)-dependent protein deacetylases that influence gene expression by forming deacetylated proteins, nicotinamide and 2'-O-acetyl-ADPR. Nicotinamide is a base-exchange substra...

journal_title：Biochemistry

authors： Sauve AA,Schramm VL

更新日期：2003-08-12 00:00:00

abstract：:The complete amino acid sequence of the 4Fe-4S ferredoxin from the thermophilic bacterium Clostridium thermoaceticum has been determined. The protein is extremely thermostable and is the only known clostridial ferredoxin to contain a single [4Fe-4S] cluster. The sequence totals 63 residues and includes the first trypt...

journal_title：Biochemistry

authors： Elliott JI,Yang SS,Ljungdahl LG,Travis J,Reilly CF

更新日期：1982-07-06 00:00:00

abstract：:The single reactive sulfhydryl group, located in the active site of each subunit of dimeric creatine kinase from rabbit muscle (isozyme MM), was selectively labeled with 3-(4-maleimidylphenyl)-7-(diethylamino)-4-methylcoumarin (CPM). Isozyme BB, purified to homogeneity from rabbit brain, was conjugated with the sulfhy...

journal_title：Biochemistry

authors： Grossman SH

更新日期：1983-11-08 00:00:00

abstract：:The method of transient electric birefringence has been applied to study the conformation of protein-sodium dodecyl sulfate complexes. A model of a deformable prolate ellipsoid has been proposed for the protein-dodecyl sulfate complex. This model is compared to the models proposed by J. A. Reynolds and C. Tanford (197...

journal_title：Biochemistry

authors： Wright AK,Thompson MR,Miller RL

更新日期：1975-07-15 00:00:00

abstract：:Deletion of Glu146, Thr147, and Trp148 from thrombin dramatically alters its interactions with substrates, ligands, and inhibitors, and the changes appear to result from nonlocal modification of thrombin's structure [Le Bonniec, B. F., Guinto, E. R., & Esmon, C. T. (1992) J. Biol. Chem. 267, 19341-19348]. In an attemp...

journal_title：Biochemistry

authors： Le Bonniec BF,Betz A,Guinto ER,Esmon CT,Stone SR

更新日期：1994-04-05 00:00:00

abstract：:To understand relationships between membrane-binding properties of cytolytic peptides and resulting cytotoxicity, we investigated interactions of dermaseptin analogues with model bilayers by means of surface plasmon resonance. First, we tested the system by comparing two native dermaseptins, S1 and S4, whose binding p...

journal_title：Biochemistry

authors： Gaidukov L,Fish A,Mor A

更新日期：2003-11-11 00:00:00

abstract：:Multielectron multiproton reactions play an important role in both biological systems and chemical reactions involved in energy storage and manipulation. A key strategy employed by nature in achieving such complex chemistry is the use of proton-coupled redox steps. Cytochrome c nitrite reductase (ccNiR) catalyzes the ...

journal_title：Biochemistry

authors： Judd ET,Stein N,Pacheco AA,Elliott SJ

更新日期：2014-09-09 00:00:00

abstract：:The chromatin structure encompassing the lysozyme gene domain in hen oviduct nuclei was studied by measuring the partitioning of coding and flanking sequences during chromatin fractionation and by analyzing the nucleosome repeat in response to micrococcal nuclease digestion. Following micrococcal nuclease digestion, n...

journal_title：Biochemistry

authors： Strätling WH,Dölle A,Sippel AE

更新日期：1986-01-28 00:00:00

abstract：:The chicken and goose egg white lysozymes (ChEWL and GoEWL) are homologues, but differ in substrate specificity. ChEWL catalyzes the hydrolysis of the glycosidic bonds of bacterial peptidoglycans and chitin-derived substrates, while GoEWL is specific for bacterial peptidoglycans. The active-site aspartate 52 residue o...

journal_title：Biochemistry

authors： Matsumura I,Kirsch JF

更新日期：1996-02-13 00:00:00

abstract：:The transformation of DMPC vesicles containing myristic acid to larger structures was examined by nuclear magnetic resonance techniques. The process was found to be characterized by a rate which is approximately first order in vesicle concentration. Rates, however, show a dramatic increase with increasing myristic aci...

journal_title：Biochemistry

authors： Kantor HL,Prestegard JH

更新日期：1978-08-22 00:00:00

abstract：:Whereas the salt-dependent conformational transition of acid-denatured horse ferricytochrome c at pH 2 is approximated by a two-state mechanism from the acid-unfolded state to the molten globule state [Kataoka, M., Hagihara, Y., Mihara, K., & Goto, Y. (1993) J. Mol. Biol. 229, 591-596], the corresponding transition in...

journal_title：Biochemistry

authors： Goto Y,Hagihara Y,Hamada D,Hoshino M,Nishii I

更新日期：1993-11-09 00:00:00

abstract：:A series of 24-homologated 1,25-dihydroxyvitamin D3 compounds have been chemically synthesized and studied with regard to their activity in inducing differentiation of human promyelocyte HL-60 cells to monocytes and in calcium mobilizing activity in vitamin D deficient rats. Homologation of 1,25-dihydroxyvitamin D3 or...

journal_title：Biochemistry

authors： Perlman K,Kutner A,Prahl J,Smith C,Inaba M,Schnoes HK,DeLuca HF

更新日期：1990-01-09 00:00:00

abstract：:In aspartate aminotransferase, pyridoxal 5'-phosphate (PLP) forms a Schiff base with the epsilon-amino group of Lys258 (internal aldimine). The internal aldimine has a pKa value of 6.8. Binding of a substrate amino acid to the enzyme yields the Michaelis complex, in which PLP still forms the internal aldimine with Lys...

journal_title：Biochemistry

authors： Hayashi H,Mizuguchi H,Kagamiyama H

更新日期：1998-10-27 00:00:00

abstract：:RXPA380 (Cbz-PhePsi[PO(2)CH]Pro-Trp-OH) was reported recently as the first highly selective inhibitor of the C-domain of somatic angiotensin-converting enzyme (ACE), able to differentiate the two active sites of somatic ACE by a selectivity factor of more than 3 orders of magnitude. The contribution of each RXPA380 re...

journal_title：Biochemistry

authors： Georgiadis D,Cuniasse P,Cotton J,Yiotakis A,Dive V

更新日期：2004-06-29 00:00:00

abstract：:The cystic fibrosis transmembrane conductance regulator forms an anion-selective channel. We previously showed that charge selectivity, the ability to discriminate between anions and cations, occurs near the cytoplasmic end of the channel. The molecular determinants of charge selectivity, however, are unknown. We inve...

journal_title：Biochemistry

authors： Guinamard R,Akabas MH

更新日期：1999-04-27 00:00:00

abstract：:The reverse transcriptase (RT) from the human immunodeficiency virus (HIV) is initially expressed as a 66-kDa protein and is subsequently proteolytically processed in vivo to form a 66-kDa/51-kDa heterodimer. Comparison of circular dichroism spectra of the 66-kDa, 51-kDa, and heterodimeric forms of RT indicates that t...

journal_title：Biochemistry

authors： Anderson SF,Coleman JE

更新日期：1992-09-08 00:00:00