Abstract：
:Complement components C5b-6 and C7 assemble to form C5b-7, which then interacts with membranes and commits the membrane attack complex to a target site. This protein-membrane association event was investigated to determine possible structural features that could contribute to a selective membrane attack. This system may also suggest general properties of protein-membrane insertion events. Initial binding of C5b-6 to membranes could potentially determine the site of assembly. However, binding of C5b-6 to membranes required phosphatidylglycerol or phosphatidic acid produced from egg phosphatidylcholine while binding of C5b-6 to phosphatidylcholine, phosphatidylserine, or phosphatidylinositol was undetectable. Binding to phosphatidic acid was irreversible, and the bound C5b-6 could no longer interact with C7. In contrast, C5b-7 interacted with all phospholipids tested. The rate-limiting process was the interaction of C5b-6 and C7, which displayed bimolecular properties and an activation energy of 37 kcal/mol. The C5b-7 complex showed 20-fold selectivity for small unilamellar phospholipid vesicles over large unilamellar vesicles. Vesicles carrying high negative charge densities were selected over neutral vesicles by a factor of about 5. Vesicles formed from phospholipids with short, saturated hydrocarbon side chains (dimyristoylphosphatidylcholine and dipalmitoylphosphatidylcholine) were about 5-fold less effective than those formed from phospholipids with natural fatty acid distributions. The gel vs. fluid state had little influence on C5b-7 insertion.(ABSTRACT TRUNCATED AT 250 WORDS)
journal_name
Biochemistryjournal_title
Biochemistryauthors
Silversmith RE,Nelsestuen GLdoi
10.1021/bi00371a065subject
Has Abstractpub_date
1986-11-18 00:00:00pages
7717-25issue
23eissn
0006-2960issn
1520-4995journal_volume
25pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract：:The role of the tryptophan residues in the substrate-binding and catalytic mechanism of an enzymatically active C-terminal fragment of Pseudomonas aeruginosa exotoxin A was studied by individually or jointly replacing these residues with phenylalanine. Substitution of W-466 decreased the ADP-ribosyltransferase and NAD...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi961985t
更新日期：1996-12-03 00:00:00
abstract：:DNA polymerase (pol) β is a small eukaryotic DNA polymerase composed of two domains. Each domain contributes an enzymatic activity (DNA synthesis and deoxyribose phosphate lyase) during the repair of simple base lesions. These domains are termed the polymerase and lyase domains, respectively. Pol β has been an excelle...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi500139h
更新日期：2014-05-06 00:00:00
abstract：:Protease inhibition by metastable serine protease inhibitors (serpins) is mediated by one of the largest functional intradomain conformational changes known in biology. In this extensive structural rearrangement, protease-serpin complex formation triggers cleavage of the serpin reactive center loop (RCL), its subseque...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.6b00839
更新日期：2017-01-31 00:00:00
abstract：:PHD2 is a 2-oxoglutarate, non-heme Fe(2+)-dependent oxygenase that senses O2 levels in human cells by hydroxylating two prolyl residues in the oxygen-dependent degradation domain (ODD) of HIF1α. Identifying the active site contacts that determine the rate of reaction at limiting O2 concentrations is crucial for unders...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi501540c
更新日期：2015-05-12 00:00:00
abstract：:The Type I homodimeric photosynthetic reaction center found in anaerobic gram-positive bacteria of the genus Heliobacteriaceae incorporates FA- and FB-like iron-sulfur clusters similar to those found in Photosystem I as terminal electron acceptors. We recently isolated the PshB protein that harbors the iron-sulfur clu...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0622165
更新日期：2007-03-06 00:00:00
abstract：:The beta-glucosidase from Agrobacterium faecalis (previously Alcaligenes faecalis) has been subjected to a detailed kinetic investigation with a range of substrates to probe its specificity and mechanism. It has a relatively broad specificity for the substrate sugar moiety and exhibits a classical pH dependence for it...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00156a015
更新日期：1992-10-20 00:00:00
abstract：:The turnover of the collagen triple-helical structure (collagenolysis) is a tightly regulated process in normal physiology and has been ascribed to a small number of proteases. Several members of the matrix metalloproteinase (MMPs) family possess collagenolytic activity, and the mechanisms by which these enzymes proce...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi062199j
更新日期：2007-03-27 00:00:00
abstract：:The temperature dependence of the reduction potential of the CuA site in carbon monoxide inhibited cytochrome c oxidase has been measured with a spectroelectrochemical method adapted to the relatively weak near-infrared absorption of this copper ion. These measurements, together with parallel measurements on the 604-n...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00349a024
更新日期：1986-01-14 00:00:00
abstract：:von Willebrand A domain-containing protein 8 (VWA8) is a poorly characterized, mitochondrial matrix-targeted protein with an AAA ATPase domain and ATPase activity that increases in livers of mice fed a high-fat diet. This study was undertaken to use CRISPR/Cas9 to delete VWA8 in cultured mouse hepatocytes and gain ins...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.9b00863
更新日期：2019-12-10 00:00:00
abstract：:The solution conformation of the DNA duplex d(C1G2C3A4C5L6C7A8C9G10C11).d(G12C13G14T15G16T17G18T19G20C21G22 ) containing the 2'-deoxyribonolactone lesion (L6) in the middle of the sequence has been investigated by NMR spectroscopy and restrained molecular dynamics calculations. Interproton distances have been obtained...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi982743r
更新日期：1999-03-30 00:00:00
abstract：:Intermolecular triplex DNA is stabilized by metal cations and polyamines which reduce repulsion between the negatively charged phosphates of the three nucleic acid strands. We use a quantitative chemical-probing assay involving protection of duplex guanines in a homopyrimidine.homopurine (Py.Pu) sequence from dimethyl...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00045a033
更新日期：1995-11-14 00:00:00
abstract：:In order to elucidate the molecular mechanisms responsible for the apparent nonlinear behavior of the temperature dependence of the redox potential of Hydrogenobacter thermophilus cytochrome c552 [Takahashi, Y., Sasaki, H., Takayama, S. J., Mikami, S., Kawano, S., Mita, H., Sambongi, Y., and Yamamoto, Y. (2006) Bioche...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi7000714
更新日期：2007-08-14 00:00:00
abstract：:The capsular polysaccharide (CPS) structure of Campylobacter jejuni contributes to its robust fitness. Many strains contain heptose moieties in their CPS units. The precursor heptose is GDP-d-glycero-α-d-manno-heptose; modifications to the stereochemistry at C3-C6 as well as additions of methyl and phosphoramidate gro...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.9b00548
更新日期：2019-09-17 00:00:00
abstract：:The effects of Ca2+, Mg2+, and troponin I (TnI) inhibitory peptide (Ip) binding on the spectral properties of a Phe-154 to Trp mutant (F154W) of chicken recombinant troponin C (rTnC) have been examined. Residue 154 is positioned in the final flanking helix H of metal binding site IV. Since there are no other Tyr or Tr...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00176a028
更新日期：1994-03-15 00:00:00
abstract：:Aminoacyl-tRNA synthetases ensure the fidelity of protein synthesis by accurately selecting and activating cognate amino acids for aminoacylation of the correct tRNA. Some tRNA synthetases have evolved an editing active site that is separate from the amino acid activation site providing two steps or "sieves" for amino...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi002915w
更新日期：2001-05-08 00:00:00
abstract：:The primary structure of a multifunctional protein, the large alpha-subunit of the Escherichia coli fatty acid oxidation complex, was determined by sequencing the fadB region of the fadBA operon. The amino-terminal sequence of this protein had been established by Edman degradation. The transcription start site of the ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00241a023
更新日期：1991-07-09 00:00:00
abstract：:Terreic acid is a metabolite with antibiotic properties produced by the fungus Aspergillus terreus. We found that terreic acid is a covalent inhibitor of the bacterial cell wall biosynthetic enzyme MurA from Enterobacter cloacae and Escherichia coli in vitro. The crystal structure of the MurA dead-end complex with ter...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi100365b
更新日期：2010-05-18 00:00:00
abstract：:Lactone-hydrolyzing enzymes derived from some Bacillus species are capable of disrupting quorum sensing in bacteria that use N-acyl-l-homoserine lactones (AHLs) as intercellular signaling molecules. Despite the promise of these quorum-quenching enzymes as therapeutic and anti-biofouling agents, the ring opening mechan...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi061238o
更新日期：2006-11-07 00:00:00
abstract：:The positions of single amino acids in the interhelical loop regions and the C-terminal tail of bacteriorhodopsin (bR) were investigated by X-ray diffraction using site-directed heavy-atom labeling. Since wild-type bR does not contain any cysteines, appropriate cysteine mutants were produced with a unique sulfhydryl g...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi971735s
更新日期：1998-07-21 00:00:00
abstract：:Holliday junctions are central intermediates in the process of genetic recombination. These DNA molecules contain four double helices that flank a central branch point, so that each of the four strands that constitute the junction is associated with two different helices. Previously, we have shown that such branched j...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00003a026
更新日期：1995-01-24 00:00:00
abstract：:We have synthesized 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho(N-oleoyl)serine (N-acyl-PS) and 1,2-dioleoyl-sn-glycero-3-phospho-Tris (phosphatidyl-Tris) and have characterized both phospholipids by their chemical and chromatographic properties, as well as by their IR, 13C NMR, and 1H NMR spectra. Comparison of these d...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00221a003
更新日期：1991-02-19 00:00:00
abstract：:Single-strand-specific nucleases are a diverse and important group of enzymes that are able to cleave a variety of DNA structures present in duplex molecules. Nuclease SP, an enzyme from spinach, has been purified to apparent homogeneity, allowing for the unambiguous characterization of a number of its physical proper...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00104a026
更新日期：1991-10-08 00:00:00
abstract：:Phthalate dioxygenase from Pseudomonas cepacia contains a mononuclear ferrous center that is strictly required for catalytic oxygen activation. The spectroscopic characterization of this iron site and its ligand interactions has been complicated in the past by interference from a Rieske-type binuclear (2Fe-2S) cluster...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00069a017
更新日期：1993-05-11 00:00:00
abstract：:The complete amino acid sequence of the major component myoglobin from the dwarf sperm whale, Kogia simus, was determined by specific cleavage of the protein to obtain large peptides which are readily degraded by the automatic sequenator. Three easily separable peptides were obtained by cleaving the protein at its two...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00624a010
更新日期：1977-03-08 00:00:00
abstract：:Glucocorticoid receptors of wild-type and nti ("increased nuclear transfer") mutant S49.1 mouse lymphoma cells exist in extracts under low-salt conditions predominantly as high molecular weight species (Mr greater than or equal to 300,000). These receptor-hormone complexes are unable to bind to DNA. High salt (300 mM ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00415a030
更新日期：1988-07-26 00:00:00
abstract：:Whole tRNA preparation obtained from a human cell line (HT-29) of colon carcinoma and purified specific Escherichia coli tRNA were reacted with pyridoxal 5'-phosphate, reduced by sodium borohydride and digested with RNase A and snake venom phosphodiesterase. Two-dimensional chromatography of the pyridoxal 5'-phosphate...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00635a033
更新日期：1977-08-09 00:00:00
abstract：:The calcitonin gene-related peptide (CGRP) receptor is a heterodimer of two membrane proteins: calcitonin receptor-like receptor (CLR) and receptor activity-modifying protein 1 (RAMP1). CLR is a class B G-protein-coupled receptor (GPCR), possessing a characteristic large amino-terminal extracellular domain (ECD) impor...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi901848m
更新日期：2010-03-09 00:00:00
abstract：:ABCG2 is a half-ATP binding cassette (ABC) drug transporter that consists of a nucleotide binding domain (NBD) followed by a transmembrane domain. This half-ABC transporter is thought to form a homodimer in the plasma membrane where it transports anticancer drugs across the biological membranes in an ATP-dependent man...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi900675v
更新日期：2009-09-29 00:00:00
abstract：:Endonuclease V from bacteriophage T4 is a well characterized enzyme that initiates the repair of ultraviolet light induced pyrimidine dimers. Scission of the phosphodiester backbone between the pyrimidines within a dimer, or 3' to an abasic (AP) site, occurs by a beta-elimination mechanism. In addition, high concentra...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00027a031
更新日期：1995-07-11 00:00:00
abstract：:The intercellular environment is known to be very different from the environment where most of the elementary biological processes are studied in the laboratory. As a result, there was a considerable effort on cell mimicking either by confinement or by introducing macromolecular crowding. In the present study, dextran...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.8b00599
更新日期：2018-10-23 00:00:00
