Effects of phosphorylation of serine 40 of tyrosine hydroxylase on binding of catecholamines: evidence for a novel regulatory mechanism.

abstract：:Histidine-containing protein, HPr, is a phosphocarrier protein that is part of the bacterial phosphoenolpyruvate:sugar phosphotransferase system. HPr is phosphorylated by enzyme I, and P-HPr transfers the phosphoryl group to the IIA domain of a number of sugar-specific enzyme II complexes. Autocatalytic phosphoryl tra...

journal_title：Biochemistry

authors： Anderson JW,Waygood EB

更新日期：1993-06-08 00:00:00

abstract：:A comparison of the oxidations of diclofenac with microsomes of yeasts expressing various human liver cytochromes P450 showed that P450 2C9 regioselectively led to 4'-hydroxy diclofenac (4'-OHD) whereas P450 3A4 only led to 5-hydroxy diclofenac (5-OHD). P450 2C19, 2C18, and 2C8 led to the simultaneous formation of 4'-...

journal_title：Biochemistry

authors： Mancy A,Antignac M,Minoletti C,Dijols S,Mouries V,Duong NT,Battioni P,Dansette PM,Mansuy D

更新日期：1999-10-26 00:00:00

abstract：:The role of the Na,K-ATPase beta-subunit in stabilization of ion-binding sites has been investigated. Treatment of the purified renal Na,K-ATPase with 0.25 M DTT at 40 degrees C for 1 h resulted in 50% loss of Rb occlusion, which correlates with partial reduction of S-S bridges in the extracellular portion of the beta...

journal_title：Biochemistry

authors： Lutsenko S,Kaplan JH

更新日期：1993-07-06 00:00:00

abstract：:Regulation of Saccharomyces cerevisiae membrane-associated phosphatidate phosphatase (3-sn-phosphatidate phosphohydrolase, EC 3.1.3.4) activity by phospholipids was examined using purified enzyme and Triton X-100/phospholipid-mixed micelles. Anionic phospholipids activated phosphatidate phosphatase activity whereas zw...

journal_title：Biochemistry

authors： Wu WI,Carman GM

更新日期：1996-03-26 00:00:00

abstract：:The turnover of the collagen triple-helical structure (collagenolysis) is a tightly regulated process in normal physiology and has been ascribed to a small number of proteases. Several members of the matrix metalloproteinase (MMPs) family possess collagenolytic activity, and the mechanisms by which these enzymes proce...

journal_title：Biochemistry

authors： Minond D,Lauer-Fields JL,Cudic M,Overall CM,Pei D,Brew K,Moss ML,Fields GB

更新日期：2007-03-27 00:00:00

abstract：:Proteolytic degradation of glutamine synthetase (GS) in Escherichia coli is known to follow "marking" by oxidative modification. At an early stage of the degradative pathway, oxidation of His 269 and Arg 344 abolishes GS enzymatic activity. We propose a mechanism for the early stage of oxidative inactivation of GS on ...

journal_title：Biochemistry

authors： Liaw SH,Villafranca JJ,Eisenberg D

更新日期：1993-08-10 00:00:00

abstract：:The amide hydrogens that are exposed to solvent in the high-resolution X-ray structures of ubiquitin, FK506-binding protein, chymotrypsin inhibitor 2, and rubredoxin span a billion-fold range in hydroxide-catalyzed exchange rates which are predictable by continuum dielectric methods. To facilitate analysis of transien...

journal_title：Biochemistry

authors： LeMaster DM,Anderson JS,Hernández G

更新日期：2009-10-06 00:00:00

abstract：:Bacillus thuringiensis (Bt) insecticidal toxins bind to receptors on midgut epithelial cells of susceptible insects, and binding triggers biochemical events that lead to insect mortality. Recently, a 100-kDa aminopeptidase N (APN) was isolated from brush border membrane vesicles (BBMV) of Anopheles quadrimaculatus and...

journal_title：Biochemistry

authors： Zhang R,Hua G,Andacht TM,Adang MJ

更新日期：2008-10-28 00:00:00

abstract：:The three-dimensional structure of the bifunctional alpha-amylase/trypsin inhibitor (RBI) from seeds of ragi (Eleusine coracana Gaertneri) has been determined in solution using multidimensional 1H and 15N NMR spectroscopy. The inhibitor consists of 122 amino acids, with 5 disulfide bridges, and belongs to the plant al...

journal_title：Biochemistry

authors： Strobl S,Mühlhahn P,Bernstein R,Wiltscheck R,Maskos K,Wunderlich M,Huber R,Glockshuber R,Holak TA

更新日期：1995-07-04 00:00:00

abstract：:Electron donation from the soluble cytochrome (cyt) c2 to the photooxidized primary donor, P+, of reaction centers isolated from Rhodobacter sphaeroides was studied by using chemical zero-length cross-linking. This cross-linking stabilizes a 1:1 covalent complex between subunit M of the reaction center and cyt c2. In ...

journal_title：Biochemistry

authors： Drepper F,Dorlet P,Mathis P

更新日期：1997-02-11 00:00:00

abstract：:For the study of the protein--protein and protein--nucleic acid interactions in the assembly of virus particles, laser Raman spectra have been obtained in H2O and D2O solutions and as a function of temperature for the following Salmonella phage P22 components: mature phage particles, isolated mature phage DNA, mature ...

journal_title：Biochemistry

authors： Thomas GJ Jr,Li Y,Fuller MT,King J

更新日期：1982-08-03 00:00:00

abstract：:The arrangement of the mRNA on the Escherichia coli ribosome with respect to ribosomal RNA sites has been investigated by photochemical cross-linking experiments. mRNA analogues 51-54 nucleotides in length contained a Shine-Dalgarno sequence, a single codon for tRNA(Gly), and 4-thiouridine (s4U) in the 5' third of the...

journal_title：Biochemistry

authors： Bhangu R,Juzumiene D,Wollenzien P

更新日期：1994-03-15 00:00:00

abstract：:Saccharomyces cerevisiae myristoylCoA:protein N-myristoyltransferase (Nmt1p) is an essential enzyme that catalyzes the transfer of myristic acid (C14:0) from myristoylCoA to the N-terminus of cellular proteins with a variety of functions. Nmts from an assortment of species display remarkable in vivo specificity for th...

journal_title：Biochemistry

authors： Bhatnagar RS,Schall OF,Jackson-Machelski E,Sikorski JA,Devadas B,Gokel GW,Gordon JI

更新日期：1997-06-03 00:00:00

abstract：:Electrophilic halogenating agents, including hypohalous acids and haloamines, oxidize free methionine and the N-terminal methionines of peptides and proteins (e.g., Met-1 of anti-inflammatory peptide 1 and ubiquitin) to produce dehydromethionine (a five-membered isothiazolidinium heterocycle). Amide derivatives of met...

journal_title：Biochemistry

authors： Beal JL,Foster SB,Ashby MT

更新日期：2009-11-24 00:00:00

abstract：:Ionizing radiation, free radicals, and reactive oxygen species produce hundreds of different DNA lesions. Clustered lesions are typical for ionizing radiation. They compromise the efficiency of the base excision repair (BER) pathway, and as a consequence, they are much more toxic and mutagenic than isolated lesions. D...

journal_title：Biochemistry

authors： Zálešák J,Constant JF,Jourdan M

更新日期：2016-07-19 00:00:00

abstract：:The preferential interactions of proteins with solvent components were studied in concentrated aqueous solutions of the sulfate, acetate, and chloride salts of Mg2+, Ba2+, Ca2+, Mn2+ and Ni2+ [except for CaSO4, BaSO4, Mn-(OAc)2, and Ni(OAc)2], and results were compared with those of the Na+ salts. It was found that, f...

journal_title：Biochemistry

authors： Arakawa T,Timasheff SN

更新日期：1984-12-04 00:00:00

abstract：:The crystal structure of a ternary complex of the alcohol dehydrogenase from the archaeon Sulfolobus solfataricus (SsADH) has been determined at 2.3 A. The asymmetric unit contains a dimer with a NADH and a 2-ethoxyethanol molecule bound to each subunit. The comparison with the apo structure of the enzyme reveals that...

journal_title：Biochemistry

authors： Esposito L,Bruno I,Sica F,Raia CA,Giordano A,Rossi M,Mazzarella L,Zagari A

更新日期：2003-12-16 00:00:00

abstract：:We used CD and UV resonance Raman spectroscopy to study the impact of alcohols on the conformational equilibria and relative Gibbs free energy landscapes along the Ramachandran Psi-coordinate of a mainly poly-Ala peptide, AP with an AAAAA(AAARA)(3)A sequence. 2,2,2-Trifluoroethanol (TFE) most stabilizes the alpha-heli...

journal_title：Biochemistry

authors： Xiong K,Asher SA

更新日期：2010-04-20 00:00:00

abstract：:The effect of the general anesthetics alpha-chloralose and chloral hydrate as well as the nonanesthetic beta-chloralose on the order of phosphatidylcholine and phosphatidylcholine-cholesterol liposomes has been examined by 2H nuclear magnetic resonance. Chloral hydrate interacts with the hydrophilic head-group region,...

journal_title：Biochemistry

authors： Forrest BJ,Mattai J

更新日期：1985-12-03 00:00:00

abstract：:We have biochemically and kinetically characterized the polymerase and exonuclease activities of the third B-family polymerase (Dpo3) from the hyperthermophilic Crenarchaeon, Sulfolobus solfataricus (Sso). We have established through mutagenesis that despite incomplete sequence conservation, the polymerase and exonucl...

journal_title：Biochemistry

authors： Bauer RJ,Begley MT,Trakselis MA

更新日期：2012-03-06 00:00:00

abstract：:Plants employ a variety of light, oxygen, voltage (LOV) domain photoreceptors to regulate diverse aspects of growth and development. The Zeitlupe (ZTL), Flavin-Kelch-Fbox-1 (FKF1), and LOV-Kelch-Protein-2 (LKP2) proteins dictate measurement of the day length, flowering time, and regulation of the circadian clock by bl...

journal_title：Biochemistry

authors： Pudasaini A,Zoltowski BD

更新日期：2013-10-08 00:00:00

abstract：:The contribution of backbone hydrogen bonds in alpha-helices to the overall stability of a protein has been examined experimentally by replacing several backbone amide linkages in alpha-helix 39-50 of T4 lysozyme with ester linkages. T4 lysozyme variants wherein the backbone amide bonds between residues Ser38 and Leu3...

journal_title：Biochemistry

authors： Koh JT,Cornish VW,Schultz PG

更新日期：1997-09-23 00:00:00

abstract：:We describe a real-time assay for the interaction of the G-protein of retinal rods, transducin (Gt), with its effector, cGMP phosphodiesterase (PDE). The purified proteins were recombined with isolated rod disk membranes. Reactions were triggered by flash photolysis of the receptor rhodopsin, and monitored by changes ...

journal_title：Biochemistry

authors： Heck M,Hofmann KP

更新日期：1993-08-17 00:00:00

abstract：:Lactyl-CoA dehydratase consists of two enzymes, E1 and E2, and requires catalytic quantities of ATP for activity [Kuchta, R. D., & Abeles, R. H. (1985) J. Biol. Chem. 260, 13181-13189]. In contrast to E1, which contains no Fe, E2 contains 8.20 +/- 0.04 mol of Fe/mol of E2, one of which can be removed by 1,10-phenanthr...

journal_title：Biochemistry

authors： Kuchta RD,Hanson GR,Holmquist B,Abeles RH

更新日期：1986-11-18 00:00:00

abstract：:Merozoite surface protein 2 (MSP2), an abundant glycosylphosphatidylinositol-anchored protein on the surface of Plasmodium falciparum merozoites, is a promising malaria vaccine candidate. MSP2 is intrinsically disordered and forms amyloid-like fibrils in solution under physiological conditions. The 25 N-terminal resid...

journal_title：Biochemistry

authors： Zhang X,Adda CG,Low A,Zhang J,Zhang W,Sun H,Tu X,Anders RF,Norton RS

更新日期：2012-02-21 00:00:00

abstract：:Prodan [6-propionyl-2-(dimethylamine)naphthalene] is a hydrophobic fluorescent probe which is extremely sensitive to both the polarity and the hydrogen-bond donating capacity of the solvent. In binary mixtures of solvents, the hydrogen-bond donating effect on Prodan fluorescence saturates at relatively low concentrati...

journal_title：Biochemistry

authors： Rottenberg H

更新日期：1992-10-06 00:00:00

abstract：:Neisseria meningitidis α-1,4-galactosyltransferase C (LgtC) is responsible for the transfer of α-galactose from donor UDP-galactose to the lipooligosaccharide terminal acceptor lactose. Crystal structures of its substrate analogue complexes have provided key insights into the galactosyl transfer mechanism, including a...

journal_title：Biochemistry

authors： Chan PH,Cheung AH,Okon M,Chen HM,Withers SG,McIntosh LP

更新日期：2013-01-15 00:00:00

abstract：:The Iowa point mutation in apolipoprotein A-I (G26R) leads to a systemic amyloidosis condition, and the Milano mutation (R173C) is associated with hypoalphalipoproteinemia, a reduced plasma level of high-density lipoprotein. To probe the structural effects that lead to these outcomes, we used amide hydrogen-deuterium ...

journal_title：Biochemistry

authors： Chetty PS,Ohshiro M,Saito H,Dhanasekaran P,Lund-Katz S,Mayne L,Englander W,Phillips MC

更新日期：2012-11-06 00:00:00

abstract：:Acidic fibroblast growth factor (aFGF) is a signal peptide-less protein that is secreted into the extracellular compartment as part of a multiprotein release complex, consisting of aFGF, S100A13 (a calcium binding protein), and a 40 kDa (p40) form of synaptotagmin (Syt1), a protein that participates in the docking of ...

journal_title：Biochemistry

authors： Rajalingam D,Graziani I,Prudovsky I,Yu C,Kumar TK

更新日期：2007-08-14 00:00:00

abstract：:Through a ligand-directed tosyl (LDT) chemistry strategy using the synthetic acetogenin ligand AL1, we succeeded in the pinpoint alkynylation (-C≡CH) of Asp160 in the 49 kDa subunit of bovine complex I, which may be located in the inner part of the putative quinone binding cavity of the enzyme [Masuya, T., et al. (201...

journal_title：Biochemistry

authors： Masuya T,Murai M,Morisaka H,Miyoshi H

更新日期：2014-12-16 00:00:00