Abstract：
:Upon cleavage of the reactive thioester bonds (Cys-949-Glx-952) of tetrameric human alpha 2-macroglobulin (alpha 2M) by methylamine, one sulfhydryl group per alpha 2M subunit is exposed. These identical sulfhydryl group sites were labeled with the thiol-specific nitroxide spin-labels (1-oxy-2,2,5,5-tetramethyl-3-pyrrolin-3-yl)methyl methanethiosulfonate and (1-oxy-2,2,6,6-tetramethyl-4-piperidinyl)methyl methanethiosulfonate, a homologous series of maleimide spin-labels, and the thiol-specific fluorescent probe 2-[(4-maleimidophenyl)amino]naphthalene-6-sulfonic acid sodium salt (MANS). The ESR and fluorescence results showed that these sulfhydryl group sites were at the base of a narrow crevice that is greater than or equal to 8 A deep. Although the bound MANS fluorophore was slightly blue shifted with an enhanced quantum yield vs the free label in water, the environment of the sulfhydryl site appeared to be of a polar nature when compared with the emission maxima in several solvents of varying polarity. The Glx residue participating in the thioester linkage in the intact protein was labeled with 4-amino-2,2,6,6-tetramethylpiperidine-1-oxyl. The distance between the Glx and Cys moieties was estimated at greater than or equal to 10-25 A from double spin-labeling experiments.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Zhao BL,Musci G,Sugawara Y,Berliner LJdoi
10.1021/bi00414a053subject
Has Abstractpub_date
1988-07-12 00:00:00pages
5304-8issue
14eissn
0006-2960issn
1520-4995journal_volume
27pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract：:The complex of ribonuclease A (RNase A) with uridine vanadate (U-V), a transition-state analogue, has been studied with 51V and proton NMR spectroscopy in solution and by neutron diffraction in the crystalline state. Upon the addition of aliquots of U-V at pH 6.6, the C epsilon-H resonances of the two active-site hist...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00329a038
更新日期：1985-04-09 00:00:00
abstract：:The ATF (activating transcription factor) binding site within the E2A promoter region of adenovirus is shown to exist in a novel conformation in vitro via nuclear magnetic resonance methods. This novel conformation may be important to the protein DNA recognition process. This conformation has characteristics of both A...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00077a003
更新日期：1993-07-06 00:00:00
abstract：:The understudied nonribosomal-peptide-synthetase-independent siderophore (NIS) synthetase family has been increasingly associated with virulence in bacterial species due to its key role in the synthesis of hydroxamate and carboxylate "stealth" siderophores. We have identified a model family member, DesD, from Streptom...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.9b00899
更新日期：2020-09-22 00:00:00
abstract：:Both the male and the female of Mastomys natalensis, an African rat, have high levels of nerve growth factor (NGF) in their submaxillary glands. Mastomys high molecular weight (HMW) NGF was purified by gel filtration and ion-exchange chromatography and was compared with HMW NGF from the male mouse submaxillary gland. ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00418a007
更新日期：1988-09-06 00:00:00
abstract：:The studies reported here were undertaken to determine whether the thrombin precursors prothrombin, prethrombin 1, prethrombin 2, and Meizo thrombin interact with the fluorescent, reversible thrombin inhibitor dansylarginine N,N-(3-ethyl-1,5-pentanediyl)amide (DAPA) [Nesheim, M. E., Prendergast, F. G., & Mann, K. G. (...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00539a003
更新日期：1982-05-11 00:00:00
abstract：:The guanidine hydrochloride- (GuHCl-) induced unfolding and refolding of a recombinant domain of bovine microsomal cytochrome b(5) containing the first 104 amino acid residues has been characterized by both transient and equilibrium spectrophotometric methods. The soluble domain is reversibly unfolded and the equilibr...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi991413j
更新日期：1999-10-26 00:00:00
abstract：:Serine palmitoyltransferase (EC 2.3.1.50) catalyzes the condensation of L-serine and palmitoyl-CoA to yield 3-ketosphinganine in the first unique reaction of long-chain (sphingoid) base biosynthesis. The kinetic effects of changing the extracellular concentrations of the precursors for this pathway were studied with L...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00401a051
更新日期：1988-01-12 00:00:00
abstract：:Uniform double labeling of proteins for NMR studies can be prohibitively expensive, even with an efficient expression and purification scheme, due largely to the high cost of [13C6, 99%]glucose. We demonstrate here that uniformly (greater than 95%) 13C and 15N double-labeled proteins can be prepared for NMR structure/...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00232a017
更新日期：1991-05-07 00:00:00
abstract：:The steroidogenic acute regulatory protein (StAR), the first family member of START (StAR-related lipid transport) proteins, plays an essential role by facilitating the movement of cholesterol from the outer to inner mitochondrial membrane. Wild-type and mutant StAR binds cholesterol with similar intensity, but only w...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi801514e
更新日期：2009-02-17 00:00:00
abstract：:Diethyl pyrocarbonate (DEP) is an electrophilic reagent that is used to modify reversibly the histidine residues of proteins. Unfortunately, the lability of the acylated histidine adduct usually does not permit the isolation and identification of the modified histidine. By use of 500-MHz proton NMR spectroscopy, it ha...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00445a004
更新日期：1989-09-19 00:00:00
abstract：:The micellar properties of mixed, bovine gangliosides and purified galactosyl-N-acetylacetylgalactosaminyl (N-acetylneuraminyl) galactosylglucosylceramide were studied by gel filtration, equilibrium dialysis, and band and boundary centrifugation in sucrose gradients. The dissociation of micelles is very slow (days) in...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00573a028
更新日期：1979-03-20 00:00:00
abstract：:The beta subunits of the 143-kDa alpha2beta2 tetrameric enzyme tryptophan synthase have been labeled by L-[ring-4-19F]phenylalanine and L-[phenol-4-13C]tyrosine in an effort to monitor the positions of these residues on ligand binding. Of the 13 phenylalanine and 11 tyrosine residues in the beta subunit, only three pa...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi9518297
更新日期：1996-03-12 00:00:00
abstract：:The relative activities of 313 mutants of the gene V protein of bacteriophage f1, assayed in vivo, have been used to evaluate two approaches to predicting the effects of single amino acid substitutions on the function of a protein. First, we tested methods that only depend on the properties of the wild-type and substi...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00239a022
更新日期：1991-06-25 00:00:00
abstract：:In vitro transcription assays have been used to study the rate of ribonucleic acid (RNA) synthesis from the Escherichia coli lactose promotor mutant lacL8UV5 contained on a 203-bp (base pair) restriction fragment. The half-life of long (63-base) RNA production from heparin-resistant RNA polymerase-promotor complexes w...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00511a003
更新日期：1981-04-14 00:00:00
abstract：:Cholesterol and its hemisuccinate and sulfate derivatives are widely used in studies of purified membrane proteins but are difficult to solubilize in aqueous solution, even in the presence of detergent micelles. Other cholesterol derivatives do not form conventional micelles and lead to viscous solutions. To address t...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi101334j
更新日期：2010-11-09 00:00:00
abstract：:4-Azido-2-nitrophenyl phosphate (ANPP) was synthesized and characterized. ANPP, unlabeled or labeled by 32P, was used as a photoreactive analogue of Pi to study the Pi binding site(s) in isolated F1-ATPase and inside-out particles from beef heart mitochondria. In the dark, the phosphate bond of ANPP was cleaved by alk...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00561a013
更新日期：1980-09-30 00:00:00
abstract：:Human insulin-like growth factors I and II (hIGF-I, hIGF-II) are potent stimulators of cell and growth processes. They display high sequence similarity to both the A and B chains of insulin but contain an additional connecting C-domain, which reflects their secretion without specific packaging or precursor conversion....
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi020084j
更新日期：2002-07-30 00:00:00
abstract：:The doublet at 850 and 830 cm-1 in the Raman spectra of proteins containing tyrosyl residues has been examined as to its origin and the relation of its components to the environment of the phenyl ring, the state of the phenolic hydroxyl group, and the conformation of the amino acid backbone. Raman spectral studies on ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00693a014
更新日期：1975-11-04 00:00:00
abstract：:It is known that the proapoptotic protein Bax facilitates the formation of pores in bilayers, resulting in the release of proteins from the intermitochondrial space. We demonstrate that another consequence of the interaction of Bax with membranes is an increase in the rate of lipid transbilayer diffusion. We use two i...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi035348w
更新日期：2003-12-16 00:00:00
abstract：:Several different monoclonal antibodies (mAbs) have been actively developed in the field of Alzheimer's disease (AD) for basic science and clinical applications; however, the binding kinetics of many of the mAbs with the beta-amyloid peptides (Abeta) are poorly understood. A panel of mAbs with different Abeta recognit...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi900523q
更新日期：2009-11-03 00:00:00
abstract：:Comparison between the structures and solvent-accessible surfaces of the antigen-binding fragments of two murine anti-p-azophenylarsonate monoclonal antibodies, one bearing a major cross-reactive idiotype of A/J strain mice (36-71) and one lacking the idiotype (R19.9; Lascombe et al., 1989), highlight the structural b...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00229a023
更新日期：1991-04-16 00:00:00
abstract：:Lecithin retinol acyltransferase (LRAT) catalyzes the esterification of all-trans-retinol into all-trans-retinyl ester, an essential reaction in the vertebrate visual cycle. Since all-trans-retinyl esters are the substrates for the isomerization reaction that generates 11-cis-retinoids, this esterification reaction is...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0342416
更新日期：2003-05-27 00:00:00
abstract：:The BARD1 N-terminal RING domain binds BRCA1 while the BARD1 C-terminal ankyrin and tandem BRCT repeat domains bind CstF-50 to modulate mRNA processing and RNAP II stability in response to DNA damage. Here we characterize the BARD1 structural biochemistry responsible for CstF-50 binding. The crystal structure of the B...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi801115g
更新日期：2008-11-04 00:00:00
abstract：:Among hormone-inducible transcription factors, estrogen receptors (ERs) play important roles in tissue growth and differentiation, via either direct or indirect binding, in the nucleus, to specific DNA targets called estrogen responsive elements (EREs), or through nongenomic pathways. In humans, two estrogen receptor ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.5b00026
更新日期：2015-06-30 00:00:00
abstract：:Structures of the DNA sequences d(CCGGCGCCGG) and d(CCAGTACbr(5)UGG) are presented here as four-way Holliday junctions in their compact stacked-X forms, with antiparallel alignment of the DNA strands. Thus, the ACC-trinucleotide motif, previously identified as important for stabilizing the junction, is now extended to...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0346603
更新日期：2003-08-19 00:00:00
abstract：:Ribonuclease P2 from the thermophilic archaebacterium Sulfolobus solfataricus is a small protein (7 kDa) with a known three-dimensional structure. Inspection of the structure and molecular dynamics simulation reveal that three aromatic residues (Phe5, Phe31, and Tyr33) from the hydrophobic core have a strong van der W...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi970467v
更新日期：1997-07-22 00:00:00
abstract：:The HIV-1 fusion peptide serves as a useful model system for understanding viral/target cell fusion, at least to the lipid-mixing stage. Previous solid-state NMR studies have shown that the membrane-bound HIV-1 fusion peptide adopts an extended conformation in a lipid mixture close to that of host cells of the virus. ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0348157
更新日期：2003-10-14 00:00:00
abstract：:We have recently described a procedure for the purification of microtubule associated protein 1B (MAP1B) from calf brain [Pedrotti, B., & Islam K. (1995) Cell Motil. Cytoskeleton 30, 301-309], and this study further characterizes the purified protein and its interaction with microtubules. We show that purified MAP1B (...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi951314f
更新日期：1996-03-05 00:00:00
abstract：:The complete amino acid sequence of human liver NADPH-cytochrome P-450 reductase has been determined by microsequence analysis and mass spectrometry. The total sequence consists of 676 amino acids initiated by an amino-terminal acetyl group. There is no evidence for posttranslational modifications, including Asn-linke...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00447a054
更新日期：1989-10-17 00:00:00
abstract：:Assembly of the type I procollagen molecule begins with interactions among the C-pro alpha1(I) and C-pro alpha2(I) domains. The C-propeptide domains themselves have subdomains of distinct structures. The important questions are where chain association begins and the basis of the chain selectivity which leads to the pr...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0508338
更新日期：2005-11-22 00:00:00
